Information on EC 1.13.11.29 - stizolobate synthase

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The enzyme appears in viruses and cellular organisms

SplaateEC_Number,Commentary
EC NUMBER
COMMENTARY
1.13.11.29
-
SplaateRecommended_Name,GO_Number
RECOMMENDED NAME
GeneOntology No.
stizolobate synthase
-
SplaateReaction,Reaction_id,Commentary,IF(Commentary != '',Organism,'') ,IF(Commentary != '',Literature,'')
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
L-dopa + O2 = 4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
show the reaction diagram
-
-
-
-
SplaateReaction_Type,Organism,Commentary,Literature
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SplaatePathway,BRENDA_Link,KEGG_Link,MetaCyc_Link,Source_Database
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
betalamic acid biosynthesis
-
-
Tyrosine metabolism
-
-
SplaateSystematic_Name,Commentary_IUBMB
SYSTEMATIC NAME
IUBMB Comments
3,4-dihydroxy-L-phenylalanine:oxygen 4,5-oxidoreductase (recyclizing)
The intermediate product undergoes ring closure and oxidation, with NAD(P)+ as acceptor, to stizolobic acid. The enzyme requires Zn2+.
SplaateSynonyms,Organism,Commentary,Literature
SplaateCAS_Registry_Number,Commentary
CAS REGISTRY NUMBER
COMMENTARY
65979-39-7
-
SplaateOrganism, Commentary,Literature, Sequence_Code,Sequence_db,Textmining
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Stizolobium hassjoo
-
-
-
Manually annotated by BRENDA team
SplaateGeneral_Information, Organism, Commentary, Literature
SplaateSubstrates,Products,id,Organism_Substrates,Commentary_Substrates, Literature_Substrates, Commentary_Products, Literature_Products,Reversibility
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,4-dihydroxy-L-phenylalanine + O2
4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
show the reaction diagram
Stizolobium hassjoo
-
-
-
?
3,4-dihydroxy-L-phenylalanine + O2
4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
show the reaction diagram
Stizolobium hassjoo
-
-
-
?
3,4-dihydroxy-L-phenylalanine + O2
4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
show the reaction diagram
Stizolobium hassjoo
-
-
undergoes ring closure and oxidation to stizolobate with NAD(P)+ as acceptor
?
3,4-dihydroxy-L-phenylalanine + O2
4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
show the reaction diagram
Stizolobium hassjoo
-
-
undergoes ring closure and oxidation to stizolobate with NAD(P)+ as acceptor
?
SplaateNatural_Substrates,Natural_Products,id,Organism_Substrates,Commentary_Substrates,Literature_Substrates,Commentary_Products,Literature_Products,Reversibility
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3,4-dihydroxy-L-phenylalanine + O2
4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
show the reaction diagram
Stizolobium hassjoo
-
-
-
?
3,4-dihydroxy-L-phenylalanine + O2
4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
show the reaction diagram
Stizolobium hassjoo
-
-
-
?
SplaateCofactor,Organism,Commentary,Literature,Filename
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NAD+
Stizolobium hassjoo
-
required for reaction, less efficient than NADP+
NADP+
Stizolobium hassjoo
-
required for reaction
SplaateMetals_Ions,Organism,Commentary, Literature
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Zn2+
Stizolobium hassjoo
-
activation
SplaateInhibitors, Organism, Commentary, Literature,Filename
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
8-Hydroxyquinoline-5-sulfonate
Stizolobium hassjoo
-
90% inhibition at 0.1 mM
Al3+
Stizolobium hassjoo
-
95% inhibition at 0.1 mM
alpha,alpha'-dipyridyl
Stizolobium hassjoo
-
-
Co2+
Stizolobium hassjoo
-
65% inhibition at 0.1 mM
Cu2+
Stizolobium hassjoo
-
85% inhibition at 0.1 mM
Fe2+
Stizolobium hassjoo
-
85% inhibition at 0.1 mM
Fe3+
Stizolobium hassjoo
-
90% inhibition at 0.1 mM
glutathione
Stizolobium hassjoo
-
55% inhibition at 0.1 mM
iodoacetate
Stizolobium hassjoo
-
55% inhibition at 0.1 mM
KCN
Stizolobium hassjoo
-
-
Mg2+
Stizolobium hassjoo
-
65% inhibition at 0.1 mM
Mn2+
Stizolobium hassjoo
-
95% inhibition at 0.1 mM
Mo6+
Stizolobium hassjoo
-
65% inhibition at 0.1 mM
N-ethylmaleimide
Stizolobium hassjoo
-
45% inhibition at 0.1 mM
Neocuproine
Stizolobium hassjoo
-
55% inhibition at 0.1 mM
o-phenanthroline
Stizolobium hassjoo
-
85% inhibition at 0.1 mM
p-chloromercuribenzoate
Stizolobium hassjoo
-
80% inhibition at 0.1 mM
Salicylaldoxime
Stizolobium hassjoo
-
60% inhibition at 0.1 mM
SplaateActivating_Compound, Organism, Commentary, Literature,Filename
SplaateKM_Value,KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.39
3,4-dihydroxyphenylalanine
Stizolobium hassjoo
-
-
0.00022
NADP+
Stizolobium hassjoo
-
-
SplaateTurnover_Number, Turnover_Number_Maximum, Substrate,Organism,Commentary, Literature, Filename
SplaateKCat_KM_Value,KCat_KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
SplaateKI_Value,KI_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
SplaateIC50_Value,IC50_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
SplaateSpecific_Activity, Specific_Activity_Maximum, Organism ,Commentary, Literature
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.06
Stizolobium hassjoo
-
-
SplaatepH_Optimum, pH_Optimum_Maximum, Organism, Commentary, Literature
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.4
Stizolobium hassjoo
-
-
SplaatepH_Range,pH_Range_Maximum, Organism,Commentary, Literature
SplaateTemperature_Optimum, Temperature_Optimum_Maximum, Organism, Commentary, Literature
SplaateTemperature_Range, Temperature_Range_Maximum, Organism, Commentary, Literature
SplaatepI_Value,pI_Value_Maximum, Organism,Commentary, Literature
SplaateSource_Tissue, Organism, Commentary, Literature, Textmining
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Stizolobium hassjoo
-
etiolated
Manually annotated by BRENDA team
SplaateLocalization, Organism, Commentary, id_go, Literature, Textmining
SplaatePDB,PDB,PDB,Organism,Uniprot_ID
SplaateMolecular_Weight, Molecular_Weight_Maximum, Organism, Commentary, Literature
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
45000
Stizolobium hassjoo
-
gel filtration
396405
SplaateSubunits, Organism, Commentary, Literature
SplaatePosttranslational_Modification, Organism, Commentary, Literature
SplaateCommentary, Organism, Literature
SplaatepH_Stability,pH_Stability_Maximum, Organism, Commentary, Literature
SplaateTemperature_Stability,Temperature_Stability_Maximum, Organism, Commentary, Literature
SplaateGeneral_Stability, Organism, Literature
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
DTT stabilizes
Stizolobium hassjoo
-
SplaateOrganic_Solvent, Organism, Commentary, Literature
SplaateOxidation_Stability,Organism,Literature
SplaateStorage_Stability, Organism, Literature
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 0.1 mM DTT, stable for 3 months
Stizolobium hassjoo
-
30°C, half-life of 3-5 h
Stizolobium hassjoo
-
storage under nitrogen stabilizes
Stizolobium hassjoo
-
SplaateCommentary, Organism, Literature
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
-
Stizolobium hassjoo
-
SplaateCommentary, Organism, Literature
SplaateCommentary, Organism, Literature
SplaateEngineering, Organism, Commentary, Literature
SplaateCommentary, Organism, Literature
SplaateApplication,Organism,Commentary,Literature