Information on EC 1.13.11.18 - persulfide dioxygenase

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The expected taxonomic range for this enzyme is: Archaea, Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.13.11.18
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RECOMMENDED NAME
GeneOntology No.
persulfide dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-sulfanylglutathione + O2 + H2O = glutathione + sulfite + 2 H+
show the reaction diagram
overall reaction
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S-sulfanylglutathione + O2 = S-sulfinatoglutathione + H+
show the reaction diagram
(1a)
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S-sulfinatoglutathione + H2O = glutathione + sulfite + H+
show the reaction diagram
(1b), spontaneous
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Microbial metabolism in diverse environments
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sulfate reduction
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Sulfur metabolism
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SYSTEMATIC NAME
IUBMB Comments
S-sulfanylglutathione:oxygen oxidoreductase
An iron protein. Perthiols, formed spontaneously by interactions between thiols and elemental sulfur or sulfide, are the only acceptable substrate to the enzyme. The sulfite that is formed by the enzyme can be further converted into sulfate, thiosulfate or S-sulfoglutathione (GSSO3-) non-enzymically [2].
CAS REGISTRY NUMBER
COMMENTARY hide
37256-58-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Acidianus sp.
strain S5
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-
Manually annotated by BRENDA team
Acidianus sp. S5
strain S5
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-
Manually annotated by BRENDA team
strain DSM 700
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-
Manually annotated by BRENDA team
strain R1
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-
Manually annotated by BRENDA team
strain R1
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-
Manually annotated by BRENDA team
strain NB1-3
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-
Manually annotated by BRENDA team
gene ethe1
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strains N1 and SSO, in strain N1 increase of activity under microaerobic conditions, in strain SSO decrease of activity under micoaerobic conditions
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
ETHE1 appears to play an essential role in regulating sulfide levels in seeds, it is essential for early seed development
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5 sulfur + 2 O2 + 2 H2O + 4 H+
sulfite + thiosulfate + 2 sulfide
show the reaction diagram
sulfur + O2 + H2O
sulfite
show the reaction diagram
sulfur + O2 + H2O
thiosulfate
show the reaction diagram
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in the presence of reduced glutathione and 2,2'-dipyridyl
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?
additional information
?
-
Arabidopsis ETHE1 catalyzes the glutathione persulfide-dependent consumption of oxygen
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5 sulfur + 2 O2 + 2 H2O + 4 H+
sulfite + thiosulfate + 2 sulfide
show the reaction diagram
sulfur + O2 + H2O
sulfite
show the reaction diagram
additional information
?
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Q9C8L4
Arabidopsis ETHE1 catalyzes the glutathione persulfide-dependent consumption of oxygen
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glutathione
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Non-heme iron
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
diethyldithiocarbamate
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H2O2
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strong inhibition, reversible by subsequent addition of catalase and glutathione
Ni2+
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3 mM, 91% inhibition, 10 mM, complete inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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similar stimulating effect as 2,2' dipyridyl
2,2'-dipyridyl
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stimulation at low concentrations e.g. 0.1 mM, may protect glutathione from destruction
glutathione
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reduced from
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6.3
glutathione
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50
sulfur
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000352
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activity in extracts of aerobic grown cells
0.000952
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activity in extracts of anaerobic grown cells
7.95
recombinant enzyme, pH and temperature not specified in the publication
186.7
Acidianus sp.
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purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
Acidianus sp.
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 6
Acidianus sp.
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65
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no activity above
70
Acidianus sp.
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
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not active below
50 - 80
Acidianus sp.
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
strong ETHE1 expression occurs in the peripheral and chalazal endosperm of wild-type seeds prior to cellularization
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
Acidianus sp.
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SDS-PAGE
560000
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non denaturating PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 35000, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2,2'-dipyridyl stabilizes cofactor glutathione, activity is lost upon freezing and thawing
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, DEAE-cellulose fraction, 7 d, almost complete loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
30% ethanol, DEAE-cellulose
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recombinant enzyme from Escherichia coli
recombinant protein
Acidianus sp.
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli HB101
Acidianus sp.
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gene ethe1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
L55P
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the mutation is associated with ethylmalonic encephalopathy
additional information
generation of a ETHE1 loss-of-function mutation by DNA insertion, phenotpe overview
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine