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Information on EC 1.13.11.12 - linoleate 13S-lipoxygenase and Organism(s) Oryza sativa and UniProt Accession P38419

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IUBMB Comments
Contains nonheme iron. A common plant lipoxygenase that oxidizes linoleate and alpha-linolenate, the two most common polyunsaturated fatty acids in plants, by inserting molecular oxygen at the C-13 position with (S)-configuration. This enzyme produces precursors for several important compounds, including the plant hormone jasmonic acid. EC 1.13.11.58, linoleate 9S-lipoxygenase, catalyses a similar reaction at the second available position of these fatty acids.
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Oryza sativa
UNIPROT: P38419
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The taxonomic range for the selected organisms is: Oryza sativa
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
lipoxygenase, 15-lipoxygenase-1, soybean lipoxygenase-1, lipoxygenase-1, 13-lox, 13-lipoxygenase, lox-2, lipoxygenase-2, lipoxygenase-3, lox10, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
linoleate:oxygen oxidoreductase
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SYSTEMATIC NAME
IUBMB Comments
linoleate:oxygen 13-oxidoreductase
Contains nonheme iron. A common plant lipoxygenase that oxidizes linoleate and alpha-linolenate, the two most common polyunsaturated fatty acids in plants, by inserting molecular oxygen at the C-13 position with (S)-configuration. This enzyme produces precursors for several important compounds, including the plant hormone jasmonic acid. EC 1.13.11.58, linoleate 9S-lipoxygenase, catalyses a similar reaction at the second available position of these fatty acids.
CAS REGISTRY NUMBER
COMMENTARY hide
9029-60-1
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
linoleate + O2
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate
show the reaction diagram
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the ratio of the 13-hydroperoxy to 9-hydroperoxy fatty acid reaction is 98:2. The R/S stereoconfiguration of the product is not determined
-
?
linoleate + O2
(9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoate
show the reaction diagram
-
-
-
?
additional information
?
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cv. Aichiasahi
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
low abundance in immature seeds
Manually annotated by BRENDA team
low abundance in newly germinated seedlings
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
LOX1 is a non-conventional LOX with 13- or dual position-specific LOX activity. LOX1 is involved in the synthesis of jasmonic acid, colneleic acid, and (Z)-3-hexenal. The LOX1 product is involved in tolerance of the rice plant to wounding and brown planthopper Nilaparvata lugens attack
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
105000
1 * 105000, SDS-PAGE, His-tagged enzyme
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
1 * 105000, SDS-PAGE, His-tagged enzyme
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
soluble LOX purified with His bind kits
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene rci-1, DNA and amino acid sequence determination and analysis, realtime PCR expression analysis, the gene shows a cultivar/tissue specific expression
LOX1 ORF cloned into vector pET30a(+) for overexpression in Escherichia coli. LOX1-green GFP fusion construct, under the control of the cauliflower mosaic virus 35S promoter, expressed in Arabidopsis plants. Transgenic rice lines carrying either sense or antisense constructs under the control of a cauliflower mosaic virus 35S promoter
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
in all antisense transgenic rice lines and more than half of the sense lines the expression levels of LOX1, the levels of enzyme activity, and the levels of the endogenous LOX1 products (jasmonic acid, (Z)-3-hexenal and colneleic acid) at 6, 48, and 48 h after brown planthopper feeding respectively, are below the levels found in non-transgenic control plants
LOX1 transcripts accumulate rapidly and transiently in response to wounding or brown planthopper attack, reaching a peak 3 h after wounding and 6 h after insect feeding. Transgenic rice lines show altered LOX1 activity
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Peng, Y.-L.; Shirono, Y.; Ohta, H.; Hibino, T.; Tanaka, K.; Shibata, D.
A novel lipoxygenase from rice. Primary structure and specific expression upon incompatible infection with rice blast fungus
J. Biol. Chem.
269
3755-3761
1994
Oryza sativa (P38419), Oryza sativa
Manually annotated by BRENDA team
Wang, R.; Shen, W.; Liu, L.; Jiang, L.; Liu, Y.; Su, N.; Wan, J.
A novel lipoxygenase gene from developing rice seeds confers dual position specificity and responds to wounding and insect attack
Plant Mol. Biol.
66
401-414
2008
Oryza sativa (Q27PX2), Oryza sativa
Manually annotated by BRENDA team
Roychowdhury, M.; Li, X.; Qi, H.; Li, W.; Sun, J.; Huang, C.; Wu, D.
Functional characterization of 9-/13-LOXs in rice and silencing their expressions to improve grain qualities
BioMed Res. Int.
2016
4275904
2016
Oryza sativa (Q9FSE5), Oryza sativa
Manually annotated by BRENDA team