Reference on EC 1.12.99.6 - hydrogenase (acceptor)
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REF. 
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Adams, M.W.W.
The mechanisms of hydrogen activation and carbon monoxide binding by hydrogenase I and hydrogenase II of Clostridium pasteurianum
J. Biol. Chem.
262
15054-15061
1987
Clostridium pasteurianum
Adams, M.W.W.; Johnson, M.K.; Zambrano, I.C.; Mortenson, L.E.
On the novel hydrogen-activating iron-sulfur center of the "iron-only" hydrogenases
Biochimie
68
35-41
1986
Clostridium pasteurianum, Desulfovibrio vulgaris, Megasphaera elsdenii, Desulfovibrio vulgaris Hildenborough
Aono, S.; Kamachi, T.; Okura, I.
Characterization and thermostability of a membrane-bound hydrogenase from a thermophilic hydrogen oxidizing bacterium, Bacillus schlegelii
Biosci. Biotechnol. Biochem.
57
1177-1179
1993
Hydrogenibacillus schlegelii
Ballantine, S.P.; Boxer, D.H.
Nickel-containing hydrogenase isoenzymes from anaerobically grown Escherichia coli K-12
J. Bacteriol.
163
454-459
1985
Escherichia coli
Bernhard, M.; Buhrke, T.; Bleijlevens, B.; De Lacey, A.L.; Fernandez, V.M.; Albracht, S.P.J.; Friedrich, B.
The H2 sensor of Ralstonia eutropha. Biochemical characteristics, spectroscopic properties, and its interaction with a histidine protein kinase
J. Biol. Chem.
276
15592-15597
2001
Cupriavidus necator
Bryant, F.O.; Adams, M.W.W.
Characterization of hydrogenase from the hyperthermophilic archaebacterium, Pyrococcus furiosus
J. Biol. Chem.
264
5070-5079
1989
Pyrococcus furiosus
Burgdorf, T.; De Lacey, A.L.; Friedrich, B.
Functional analysis by site-directed mutagenesis of the NAD+-reducing hydrogenase from Ralstonia eutropha
J. Bacteriol.
184
6280-6288
2002
Cupriavidus necator
Czechowski, M.H.; He, S.H.; Nacro, M.; DerVartanian, D.V.; Peck, H.D., Jr.; LeGall, J.
A cytoplasmic nickel-iron hydrogenase with high specific activity from Desulfovibrio multispirans sp. n., a new species of sulfate reducing bacterium
Biochem. Biophys. Res. Commun.
125
1025-1032
1984
Desulfovibrio multispirans
Fauque, G.; Czechowski, M.; Berlier, Y.M.; Lespinat, P.A.; LeGall, J.; Moura, J.J.G.
Partial purification and characterization of the first hydrogenase isolated from a thermophilic sulfate-reducing bacterium
Biochem. Biophys. Res. Commun.
184
1256-1260
1992
Thermodesulfobacterium thermophilum
Fernandez, V.M.; Rao, K.K.; Fernandez, M.A.; Cammack, R.
Activation and deactivation of the membrane-bound hydrogenase from Desulfovibrio desulfuricans, Norway strain
Biochimie
68
43-48
1986
Desulfovibrio desulfuricans, Desulfovibrio desulfuricans Norway
Foerster, S.; Stein, M.; Brecht, M.; Ogata, H.; Higuchi, Y.; Lubitz, W.
Single crystal EPR studies of the reduced active site of [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki F
J. Am. Chem. Soc.
125
83-93
2003
Desulfovibrio vulgaris, Desulfovibrio vulgaris Miyazaki F
Fontecilla-Camps, J.C.; Frey, M.; Garcin, E.; Hatchikian, C.; Montet, Y.; Piras, C.; Vernede, X.; Volbeda, A.
Hydrogenase: a hydrogen-metabolizing enzyme. What do the crystal structures tell us about its mode of action?
Biochimie
79
661-666
1997
Megalodesulfovibrio gigas
Hatchikian, E.C.; Forget, N.; Bernadac, A.; Alazard, D.; Ollivier, B.
Involvement of a single periplasmic hydrogenase for both hydrogen uptake and production in some Desulfovibrio species
Res. Microbiol.
146
129-141
1995
Desulfovibrio vulgaris, Desulfovibrio vulgaris Groningen
Ishii, M.; Takishita, S.; Iwasaki, T.; Peerapornpisal, Y.; Yoshino, J.I.; Kodama, T.; Igarashi, Y.
Purification and characterization of membrane-bound hydrogenase from Hydrogenobacter thermophilus strain TK-6, an obligately autotrophic, thermophilic, hydrogen-oxidizing bacterium
Biosci. Biotechnol. Biochem.
64
492-502
2000
Hydrogenobacter thermophilus, Hydrogenobacter thermophilus TK-6 / IAM 12695
Ma, K.; Weiss, R.; Adams, M.W.W.
Characterization of hydrogenase II from the hyperthermophilic archaeon Pyrococcus furiosus and assessment of its role in sulfur reduction
J. Bacteriol.
182
1864-1871
2000
Pyrococcus furiosus
Hatchikian, E.C.; Forget, N.; Fernandez, V.M.; Williams, R.; Cammack, R.
Further characterization of the [iron]-hydrogenase from Desulfovibrio desulfuricans ATCC 7757
Eur. J. Biochem.
209
357-365
1992
Desulfovibrio desulfuricans
Meuer, J.; Bartoschek, S.; Koch, J.; Kunkel, A.; Hedderich, R.
Purification and catalytic properties of Ech hydrogenase from Methanosarcina barkeri
Eur. J. Biochem.
265
325-335
1999
Methanosarcina barkeri
Nicolet, Y.; Lemon, B.J.; Fontecilla-Camps, J.C.; Peters, J.W.
A novel FeS cluster in Fe-only hydrogenases
Trends Biochem. Sci.
25
138-143
2000
Clostridium pasteurianum, Desulfovibrio desulfuricans
Ogata, H.; Mizoguchi, Y.; Mizuno, N.; Miki, K.; Adachi, S.I.; Yasuoka, N.; Yagi, T.; Yamauchi, O.; Hirota, S.; Higuchi, Y.
Structural studies of the carbon monoxide complex of [NiFe]hydrogenase from Desulfovibrio vulgaris Miyazaki F: Suggestion for the initial activation site for dihydrogen
J. Am. Chem. Soc.
124
11628-11635
2002
Desulfovibrio vulgaris, Desulfovibrio vulgaris Miyazaki F
Seefeldt, L.C.; Arp, D.J.
Oxygen effects on the nickel- and iron-containing hydrogenase from Azotobacter vinelandii
Biochemistry
28
1588-1596
1989
Azotobacter vinelandii
-
Serebryakova, L.T.; Medina, M.; Zorin, N.A.; Gogotov, I.N.; Cammack, R.
Reversible hydrogenase of Anabaena variabilis ATCC 29413. Catalytic properties and characterization of redox centers
FEBS Lett.
383
79-82
1996
Trichormus variabilis
Sawers, R.G.; Boxer, D.H.
Purification and properties of membrane-bound hydrogenase isoenzyme 1 from anaerobically grown Escherichia coli K12
Eur. J. Biochem.
156
265-275
1986
Escherichia coli
Santiago, B.; Meyer, O.
Purification and molecular characterization of the H2 uptake membrane-bound NiFe-hydrogenase from the carboxidotrophic bacterium Oligotropha carboxidovorans
J. Bacteriol.
179
6053-6060
1997
Afipia carboxidovorans
Nijenhuis, I.; Zinder, S.H.
Characterization of hydrogenase and reductive dehalogenase activities of Dehalococcoides ethenogenes strain 195
Appl. Environ. Microbiol.
71
1664-1667
2005
Dehalococcoides mccartyi
Chardin, B.; Giudici-Orticoni, M.T.; De Luca, G.; Guigliarelli, B.; Bruschi, M.
Hydrogenases in sulfate-reducing bacteria function as chromate reductase
Appl. Microbiol. Biotechnol.
63
315-321
2003
Desulfomicrobium norvegicum, Solidesulfovibrio fructosivorans, Desulfovibrio vulgaris
Rodrigues, R.; Valente, F.M.A.; Pereira, I.A.C.; Oliveira, S.; Rodrigues-Pousada, C.
A novel membrane-bound Ech [NiFe] hydrogenase in Desulfovibrio gigas
Biochem. Biophys. Res. Commun.
306
366-375
2003
Megalodesulfovibrio gigas (Q7WT77), Megalodesulfovibrio gigas (Q7WT78), Megalodesulfovibrio gigas (Q7WT79), Megalodesulfovibrio gigas (Q7WT80), Megalodesulfovibrio gigas (Q7WT81), Megalodesulfovibrio gigas (Q7WT82)
Mishra, J.; Khurana, S.; Kumar, N.; Ghosh, A.K.; Das, D.
Molecular cloning, characterization, and overexpression of a novel [Fe]-hydrogenase isolated from a high rate of hydrogen producing Enterobacter cloacae IIT-BT 08
Biochem. Biophys. Res. Commun.
324
679-685
2004
Enterobacter cloacae, Enterobacter cloacae IIT-BT 08
George, S.J.; Kurkin, S.; Thorneley, R.N.; Albracht, S.P.
Reactions of H2, CO, and O2 with active [NiFe]-hydrogenase from Allochromatium vinosum. A stopped-flow infrared study
Biochemistry
43
6808-6819
2004
Allochromatium vinosum
Zadvorny, O.A.; Zorin, N.A.; Gogotov, I.N.; Gorlenko, V.M.
Properties of stable hydrogenase from the purple sulfur bacterium Lamprobacter modestohalophilus
Biochemistry
69
164-169
2004
Lamprobacter modestohalophilus
Jones, A.K.; Lamle, S.E.; Pershad, H.R.; Vincent, K.A.; Albracht, S.P.; Armstrong, F.A.
Enzyme electrokinetics: electrochemical studies of the anaerobic interconversions between active and inactive states of Allochromatium vinosum [NiFe]-hydrogenase
J. Am. Chem. Soc.
125
8505-8514
2003
Allochromatium vinosum
Lamle, S.E.; Albracht, S.P.; Armstrong, F.A.
The mechanism of activation of a [NiFe]-hydrogenase by electrons, hydrogen, and carbon monoxide
J. Am. Chem. Soc.
127
6595-6604
2005
Allochromatium vinosum
Coppi, M.V.; O'Neil, R.A.; Lovley, D.R.
Identification of an uptake hydrogenase required for hydrogen-dependent reduction of Fe(III) and other electron acceptors by Geobacter sulfurreducens
J. Bacteriol.
186
3022-3028
2004
Geobacter sulfurreducens
Dementin, S.; Burlat, B.; De Lacey, A.L.; Pardo, A.; Adryanczyk-Perrier, G.; Guigliarelli, B.; Fernandez, V.M.; Rousset, M.
A Glutamate is the essential proton transfer gate during the catalytic cycle of the [NiFe] hydrogenase
J. Biol. Chem.
279
10508-10513
2004
Solidesulfovibrio fructosivorans
Gross, R.; Pisa, R.; Sanger, M.; Lancaster, C.R.; Simon, J.
Characterization of the menaquinone reduction site in the diheme cytochrome b membrane anchor of Wolinella succinogenes NiFe-hydrogenase
J. Biol. Chem.
279
274-281
2004
Wolinella succinogenes
DeLacey, A.L.; Fernandez, V.M.; Rousset, M.; Cavazza, C.; Hatchikian, E.C.
Spectroscopic and kinetic characterization of active site mutants of Desulfovibrio fructosovoransNi-Fe hydrogenase
J. Biol. Inorg. Chem.
8
129-134
2003
Solidesulfovibrio fructosivorans
Pan, G.; Menon, A.L.; Adams, M.W.
Characterization of a [2Fe-2S] protein encoded in the iron-hydrogenase operon of Thermotoga maritima
J. Biol. Inorg. Chem.
8
469-474
2003
Thermotoga maritima
Laska, S.; Lottspeich, F.; Kletzin, A.
Membrane-bound hydrogenase and sulfur reductase of the hyperthermophilic and acidophilic archaeon Acidianus ambivalens
Microbiology
149
2357-2371
2003
Acidianus ambivalens
Oliveira, P.; Leitao, E.; Tamagnini, P.; Moradas-Ferreira, P.; Oxelfelt, F.
Characterization and transcriptional analysis of hupSLW in Gloeothece sp. ATCC 27152: an uptake hydrogenase from a unicellular cyanobacterium
Microbiology
150
3647-3655
2004
Gloeothece sp. (Q841J7), Gloeothece sp. (Q841J8)
Baginsky, C.; Brito, B.; Imperial, J.; Ruiz-Argueeso, T.; Palacios, J.M.
Symbiotic hydrogenase activity in Bradyrhizobium sp. (Vigna) increases nitrogen content in Vigna unguiculata plants
Appl. Environ. Microbiol.
71
7536-7538
2005
Bradyrhizobium sp.
Ureta, A.C.; Imperial, J.; Ruiz-Argueeso, T.; Palacios, J.M.
Rhizobium leguminosarum biovar viciae symbiotic hydrogenase activity and processing are limited by the level of nickel in agricultural soils
Appl. Environ. Microbiol.
71
7603-7606
2005
Rhizobium leguminosarum
Posewitz, M.C.; King, P.W.; Smolinski, S.L.; Smith, R.D.; Ginley, A.R.; Ghirardi, M.L.; Seibert, M.
Identification of genes required for hydrogenase activity in Chlamydomonas reinhardtii
Biochem. Soc. Trans.
33
102-104
2005
Chlamydomonas reinhardtii
Leul, M.; Mattsson, U.; Sellstedt, A.
Molecular characterization of uptake hydrogenase in Frankia
Biochem. Soc. Trans.
33
64-66
2005
Frankia sp.
Osz, J.; Bagyinka, C.
An autocatalytic step in the reaction cycle of hydrogenase from Thiocapsa roseopersicina can explain the special characteristics of the enzyme reaction
Biophys. J.
89
1984-1989
2005
Thiocapsa roseopersicina
Ren, Y.; Xing, X.H.; Zhang, C.; Gou, Z.
A simplified method for assay of hydrogenase activities of H2 evolution and uptake in Enterobacter aerogenes
Biotechnol. Lett.
27
1029-1033
2005
Klebsiella aerogenes
Winter, G.; Buhrke, T.; Lenz, O.; Jones, A.K.; Forgber, M.; Friedrich, B.
A model system for [NiFe] hydrogenase maturation studies: Purification of an active site-containing hydrogenase large subunit without small subunit
FEBS Lett.
579
4292-4296
2005
Cupriavidus necator
Parkin, A.; Cavazza, C.; Fontecilla-Camps, J.C.; Armstrong, F.A.
Electrochemical investigations of the interconversions between catalytic and inhibited states of the [FeFe]-hydrogenase from Desulfovibrio desulfuricans
J. Am. Chem. Soc.
128
16808-16815
2006
Desulfovibrio desulfuricans
Buhrke, T.; Lenz, O.; Krauss, N.; Friedrich, B.
Oxygen tolerance of the H2-sensing [NiFe] hydrogenase from Ralstonia eutropha H16 is based on limited access of oxygen to the active site
J. Biol. Chem.
280
23791-23796
2005
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Roseboom, W.; de Lacey, A.L.; Fernandez, V.M.; Hatchikian, E.C.; Albracht, S.P.
The active site of the [FeFe]-hydrogenase from Desulfovibrio desulfuricans. II. Redox properties, light sensitivity and CO-ligand exchange as observed by infrared spectroscopy
J. Biol. Inorg. Chem.
11
102-118
2006
Desulfovibrio desulfuricans
Schroeder, O.; Bleijlevens, B.; de Jongh, T.E.; Chen, Z.; Li, T.; Fischer, J.; Foerster, J.; Friedrich, C.G.; Bagley, K.A.; Albracht, S.P.; Lubitz, W.
Characterization of a cyanobacterial-like uptake [NiFe] hydrogenase: EPR and FTIR spectroscopic studies of the enzyme from Acidithiobacillus ferrooxidans
J. Biol. Inorg. Chem.
12
212-233
2007
Acidithiobacillus ferrooxidans
Long, M.; Liu, J.; Chen, Z.; Bleijlevens, B.; Roseboom, W.; Albracht, S.P.
Characterization of a HoxEFUYH type of [NiFe] hydrogenase from Allochromatium vinosum and some EPR and IR properties of the hydrogenase module
J. Biol. Inorg. Chem.
12
62-78
2007
Allochromatium vinosum
Kutty, R.; Bennett, G.N.
Studies on inhibition of transformation of 2,4,6-trinitrotoluene catalyzed by Fe-only hydrogenase from Clostridium acetobutylicum
J. Ind. Microbiol. Biotechnol.
33
368-376
2006
Clostridium acetobutylicum
Agrawal, A.G.; van Gastel, M.; Gaertner, W.; Lubitz, W.
Hydrogen bonding affects the [NiFe] active site of Desulfovibrio vulgaris Miyazaki F hydrogenase: a hyperfine sublevel correlation spectroscopy and density functional theory study
J. Phys. Chem. B
110
8142-8150
2006
Desulfovibrio vulgaris, Desulfovibrio vulgaris Miyazaki F
Ogata, H.; Hirota, S.; Nakahara, A.; Komori, H.; Shibata, N.; Kato, T.; Kano, K.; Higuchi, Y.
Activation process of [NiFe] hydrogenase elucidated by high-resolution X-Ray analyses: conversion of the ready to the unready state
Structure
13
1635-1642
2005
Desulfovibrio vulgaris, Desulfovibrio vulgaris Miyazaki F
Ghirardi, M.L.; Posewitz, M.C.; Maness, P.; Dubini, A.; Yu, J.; Seibert, M.
Hydrogenases and hydrogen photoproduction in oxygenic photosynthetic organisms
Annu. Rev. Plant Biol.
58
71-91
2007
Anabaena sp., Trichodesmium erythraeum, Crocosphaera watsonii, Lyngbya aestuarii (Q2EJS7), Nostoc sp. (Q3C1T8 and Q3C1T9), Gloeothece sp. (Q841J7 and Q84IJ8), Cronbergia siamensis SAG 11.82 (Q84GM3 and Q4G6A7), Crocosphaera watsonii WH8501, Cronbergia siamensis SAG 11.82 TISTR 8012 (Q84GM3 and Q4G6A7)
Jen, C.J.; Chou, C.H.; Hsu, P.C.; Yu, S.J.; Chen, W.E.; Lay, J.J.; Huang, C.C.; Wen, F.S.
Flow-FISH analysis and isolation of clostridial strains in an anaerobic semi-solid bio-hydrogen producing system by hydrogenase gene target
Appl. Microbiol. Biotechnol.
74
1126-1134
2007
Clostridium butyricum (Q1KPU9), Clostridium butyricum M1 (Q1KPU9)
Maeda, T.; Sanchez-Torres, V.; Wood, T.K.
Escherichia coli hydrogenase 3 is a reversible enzyme possessing hydrogen uptake and synthesis activities
Appl. Microbiol. Biotechnol.
76
1035-1042
2007
Escherichia coli
Maeda, T.; Sanchez-Torres, V.; Wood, T.K.
Protein engineering of hydrogenase 3 to enhance hydrogen production
Appl. Microbiol. Biotechnol.
79
77-86
2008
Escherichia coli, Escherichia coli K-12 BW25113
Hong, Y.G.; Guo, J.; Sun, G.P.
Identification of an uptake hydrogenase for hydrogen-dependent dissimilatory azoreduction by Shewanella decolorationis S12
Appl. Microbiol. Biotechnol.
80
517-524
2008
Shewanella decolorationis, Shewanella decolorationis S12
Tsygankov, A.A.; Minakov, E.A.; Zorin, N.A.; Gosteva, K.S.; Voronin, O.G.; Karyakin, A.A.
Measuring the pH dependence of hydrogenase activities
Biochemistry
72
968-973
2007
Desulfomicrobium baculatum, Thiocapsa roseopersicina, Thiocapsa roseopersicina Bbs, Desulfomicrobium baculatum 9974
Forzi, L.; Hellwig, P.; Thauer, R.K.; Sawers, R.G.
The CO and CN(-) ligands to the active site Fe in [NiFe]-hydrogenase of Escherichia coli have different metabolic origins
FEBS Lett.
581
3317-3321
2007
Escherichia coli
Lenz, O.; Zebger, I.; Hamann, J.; Hildebrandt, P.; Friedrich, B.
Carbamoylphosphate serves as the source of CN(-), but not of the intrinsic CO in the active site of the regulatory [NiFe]-hydrogenase from Ralstonia eutropha
FEBS Lett.
581
3322-3326
2007
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Hambourger, M.; Gervaldo, M.; Svedruzic, D.; King, P.W.; Gust, D.; Ghirardi, M.; Moore, A.L.; Moore, T.A.
[FeFe]-hydrogenase-catalyzed H2 production in a photoelectrochemical biofuel cell
J. Am. Chem. Soc.
130
2015-2022
2008
Clostridium acetobutylicum
Vogt, S.; Lyon, E.J.; Shima, S.; Thauer, R.K.
The exchange activities of [Fe] hydrogenase (iron-sulfur-cluster-free hydrogenase) from methanogenic archaea in comparison with the exchange activities of [FeFe] and [NiFe] hydrogenases
J. Biol. Inorg. Chem.
13
97-106
2008
Methanocaldococcus jannaschii
Bhosale, S.H.; Pant, A.; Khan, M.I.
Purification and characterization of putative alkaline [Ni-Fe] hydrogenase from unicellular marine green alga, Tetraselmis kochinensis NCIM 1605
Microbiol. Res.
164
131-137
2007
Tetraselmis sp. KSN-2002, Tetraselmis sp. KSN-2002 NCIM 1605
Shima, S.; Pilak, O.; Vogt, S.; Schick, M.; Stagni, M.S.; Meyer-Klaucke, W.; Warkentin, E.; Thauer, R.K.; Ermler, U.
The crystal structure of [Fe]-hydrogenase reveals the geometry of the active site
Science
321
572-575
2008
Methanocaldococcus jannaschii
Xing, D.; Ren, N.; Rittmann, B.
Genetic diversity of hydrogen-producing bacteria in an acidophilic ethanol-H2-coproducing system, analyzed using the [Fe]-hydrogenase gene
Appl. Environ. Microbiol.
74
1232-1239
2008
uncultured bacterium (Q2PWI0), uncultured bacterium (Q2PWI3), uncultured bacterium (Q2PWI4), uncultured bacterium (Q2PWI5), uncultured bacterium (Q2PWI6), uncultured bacterium (Q2PWI7), uncultured bacterium (Q2PWI9), uncultured bacterium (Q2PWJ0), uncultured bacterium (Q2PWJ8), uncultured bacterium (Q38IH5), uncultured bacterium (Q38IH6), uncultured bacterium (Q38IH8), uncultured bacterium (Q38IH9), uncultured bacterium (Q38II3), uncultured bacterium (Q38II4), uncultured bacterium (Q38IJ1)
Dias, A.V.; Mulvihill, C.M.; Leach, M.R.; Pickering, I.J.; George, G.N.; Zamble, D.B.
Structural and biological analysis of the metal sites of Escherichia coli hydrogenase accessory protein HypB
Biochemistry
47
11981-11991
2008
Escherichia coli
Lill, S.O.; Siegbahn, P.E.
An autocatalytic mechanism for NiFe-hydrogenase: reduction to Ni(I) followed by oxidative addition
Biochemistry
48
1056-1066
2009
Solidesulfovibrio fructosivorans
Stripp, S.; Sanganas, O.; Happe, T.; Haumann, M.
The structure of the active site H-cluster of [FeFe] hydrogenase from the green alga Chlamydomonas reinhardtii studied by X-ray absorption spectroscopy
Biochemistry
48
5042-5049
2009
Chlamydomonas reinhardtii
Mulder, D.W.; Ortillo, D.O.; Gardenghi, D.J.; Naumov, A.V.; Ruebush, S.S.; Szilagyi, R.K.; Huynh, B.; Broderick, J.B.; Peters, J.W.
Activation of HydA(DeltaEFG) requires a preformed [4Fe-4S] cluster
Biochemistry
48
6240-6248
2009
Chlamydomonas reinhardtii
Bodo, G.; Branca, R.M.; Toth, A.; Horvath, D.; Bagyinka, C.
Concentration-dependent front velocity of the autocatalytic hydrogenase reaction
Biophys. J.
96
4976-4983
2009
Thiocapsa roseopersicina
Mansure, J.J.; Hallenbeck, P.C.
Desulfovibrio vulgaris Hildenborough HydE and HydG interact with the HydA subunit of the [FeFe] hydrogenase
Biotechnol. Lett.
30
1765-1769
2008
Desulfovibrio vulgaris
Windhager, J.; Seidel, R.A.; Apfel, U.P.; Goerls, H.; Linti, G.; Weigand, W.
Oxidation of diiron and triiron sulfurdithiolato complexes: mimics for the active site of [FeFe]-hydrogenase
Chem. Biodivers.
5
2023-2041
2008
synthetic construct
Wright, J.A.; Pickett, C.J.
Protonation of a subsite analogue of [FeFe]-hydrogenase: mechanism of a deceptively simple reaction revealed by time-resolved IR spectroscopy
Chem. Commun. (Camb. )
38
5719-5721
2009
synthetic construct
Wang, N.; Wang, M.; Zhang, T.; Li, P.; Liu, J.; Sun, L.
A proton-hydride diiron complex with a base-containing diphosphine ligand relevant to the [FeFe]-hydrogenase active site
Chem. Commun. (Camb. )
44
5800-5802
2008
synthetic construct
Ichikawa, K.; Matsumoto, T.; Ogo, S.
Critical aspects of [NiFe]hydrogenase ligand composition
Dalton Trans.
22
4304-4309
2009
Megalodesulfovibrio gigas
Green, K.N.; Hess, J.L.; Thomas, C.M.; Darensbourg, M.Y.
Resin-bound models of the [FeFe]-hydrogenase enzyme active site and studies of their reactivity
Dalton Trans.
22
4344-4350
2009
synthetic construct
Pilet, E.; Nicolet, Y.; Mathevon, C.; Douki, T.; Fontecilla-Camps, J.C.; Fontecave, M.
The role of the maturase HydG in [FeFe]-hydrogenase active site synthesis and assembly
FEBS Lett.
583
506-511
2009
Chlamydomonas reinhardtii, Clostridium acetobutylicum, Desulfovibrio vulgaris, Thermotoga maritima
Hiromoto, T.; Ataka, K.; Pilak, O.; Vogt, S.; Stagni, M.S.; Meyer-Klaucke, W.; Warkentin, E.; Thauer, R.K.; Shima, S.; Ermler, U.
The crystal structure of C176A mutated [Fe]-hydrogenase suggests an acyl-iron ligation in the active site iron complex
FEBS Lett.
583
585-590
2009
Methanocaldococcus jannaschii
Yoon, K.S.; Sakai, Y.; Tsukada, N.; Fujisawa, K.; Nishihara, H.
Purification and biochemical characterization of a membrane-bound [NiFe]-hydrogenase from a hydrogen-oxidizing, lithotrophic bacterium, Hydrogenophaga sp. AH-24
FEMS Microbiol. Lett.
290
114-120
2009
Hydrogenophaga sp., Hydrogenophaga sp. AH-24
Barton, B.E.; Rauchfuss, T.B.
Terminal hydride in [FeFe]-hydrogenase model has lower potential for H2 production than the isomeric bridging hydride
Inorg. Chem.
47
2261-2263
2008
synthetic construct
Tye, J.W.; Darensbourg, M.Y.; Hall, M.B.
Refining the active site structure of iron-iron hydrogenase using computational infrared spectroscopy
Inorg. Chem.
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Desulfovibrio vulgaris, Desulfovibrio vulgaris str. Hildenborough
Justice, A.K.; De Gioia, L.; Nilges, M.J.; Rauchfuss, T.B.; Wilson, S.R.; Zampella, G.
Redox and structural properties of mixed-valence models for the active site of the [FeFe]-hydrogenase: progress and challenges
Inorg. Chem.
47
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synthetic construct
Stiebritz, M.T.; Reiher, M.
Theoretical study of dioxygen induced inhibition of [FeFe]-hydrogenase
Inorg. Chem.
48
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2009
Clostridium pasteurianum
Chiang, M.H.; Liu, Y.C.; Yang, S.T.; Lee, G.H.
Biomimetic model featuring the NH proton and bridging hydride related to a proposed intermediate in enzymatic H(2) production by Fe-only hydrogenase
Inorg. Chem.
48
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2009
synthetic construct
Olsen, M.T.; Bruschi, M.; De Gioia, L.; Rauchfuss, T.B.; Wilson, S.R.
Nitrosyl derivatives of diiron(I) dithiolates mimic the structure and Lewis acidity of the [FeFe]-hydrogenase active site
J. Am. Chem. Soc.
130
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2008
synthetic construct
Parkin, A.; Goldet, G.; Cavazza, C.; Fontecilla-Camps, J.C.; Armstrong, F.A.
The difference a Se makes? Oxygen-tolerant hydrogen production by the [NiFeSe]-hydrogenase from Desulfomicrobium baculatum
J. Am. Chem. Soc.
130
13410-13416
2008
Desulfomicrobium baculatum
Shaw, A.J.; Hogsett, D.A.; Lynd, L.R.
Identification of the [FeFe]-hydrogenase responsible for hydrogen generation in Thermoanaerobacterium saccharolyticum and demonstration of increased ethanol yield via hydrogenase knockout
J. Bacteriol.
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2009
Thermoanaerobacterium saccharolyticum
Saggu, M.; Zebger, I.; Ludwig, M.; Lenz, O.; Friedrich, B.; Hildebrandt, P.; Lendzian, F.
Spectroscopic insights into the oxygen-tolerant membrane-associated [NiFe] hydrogenase of Ralstonia eutropha H16
J. Biol. Chem.
284
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Cupriavidus necator H16
Ludwig, M.; Schubert, T.; Zebger, I.; Wisitruangsakul, N.; Saggu, M.; Strack, A.; Lenz, O.; Hildebrandt, P.; Friedrich, B.
Concerted action of two novel auxiliary proteins in assembly of the active site in a membrane-bound [NiFe] hydrogenase
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Cupriavidus necator H16
Nishimura, H.; Sako, Y.
Purification and characterization of the oxygen-thermostable hydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum camini
J. Biosci. Bioeng.
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Aeropyrum camini, Persephonella hydrogeniphila, Aeropyrum camini SY1 / ATCC BAA-758 / DSM 16960 / JCM 2091, Persephonella hydrogeniphila 29W / DSM 15103 / JCM11663
Kuchenreuther, J.M.; Stapleton, J.A.; Swartz, J.R.
Tyrosine, cysteine, and S-adenosyl methionine stimulate in vitro [FeFe] hydrogenase activation
PLoS ONE
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2009
Chlamydomonas reinhardtii
Zbell, A.; Maier, R.
Role of the Hya hydrogenase in recycling of anaerobically produced H2 in Salmonella enterica serovar Typhimurium
Appl. Environ. Microbiol.
75
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2009
Salmonella enterica, Salmonella enterica JSG210, serovar Typhimurium
Vargas, W.A.; Weyman, P.D.; Tong, Y.; Smith, H.O.; Xu, Q.
A [NiFe]-hydrogenase from Alteromonas macleodii with unusual stability in the presence of oxygen and high temperature
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2011
Alteromonas macleodii
Nishimura, H.; Kitano, Y.; Inoue, T.; Nomura, K.; Sako, Y.
Purification and characterization of membrane-associated hydrogenase from the deep-sea epsilonproteobacterium Hydrogenimonas thermophila
Biosci. Biotechnol. Biochem.
74
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2010
Hydrogenimonas thermophila, Hydrogenimonas thermophila EPI-55-1%T
Constant, P.; Chowdhury, S.P.; Pratscher, J.; Conrad, R.
Streptomycetes contributing to atmospheric molecular hydrogen soil uptake are widespread and encode a putative high-affinity [NiFe]-hydrogenase
Environ. Microbiol.
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2010
Streptomyces sp., Streptomyces sp. PCB7
Seabra, R.; Santos, A.; Pereira, S.; Moradas-Ferreira, P.; Tamagnini, P.
Immunolocalization of the uptake hydrogenase in the marine cyanobacterium Lyngbya majuscula CCAP 1446/4 and two Nostoc strains
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Nostoc punctiforme, Nostoc sp., Lyngbya majuscula, Lyngbya majuscula CCAP 1446/4, Nostoc sp. PCC 7120
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Symbiotic legume nodules employ both rhizobial exo- and endo-hydrogenases to recycle hydrogen produced by nitrogen fixation
PLoS ONE
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Azorhizobium caulinodans, Azorhizobium caulinodans ORS571, 57100
Barz, M.; Beimgraben, C.; Staller, T.; Germer, F.; Opitz, F.; Marquardt, C.; Schwarz, C.; Gutekunst, K.; Vanselow, K.H.; Schmitz, R.; LaRoche, J.; Schulz, R.; Appel, J.
Distribution analysis of hydrogenases in surface waters of marine and freshwater environments
PLoS ONE
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Synechocystis sp., Lyngbya sp., Microcystis aeruginosa, Nostoc punctiforme, Prochlorococcus marinus, Prochlorothrix hollandica, Limnospira maxima, Arthrospira platensis, Synechococcus sp., Gloeobacter violaceus, Thermosynechococcus vestitus, Synechococcus elongatus, Trichodesmium erythraeum, Crocosphaera subtropica ATCC 51142, Crocosphaera subtropica ATCC 51142 (B1WTU9), Gloeocapsa alpicola, Crocosphaera watsonii, Cronbergia siamensis SAG 11.82, Acaryochloris marina, Cylindrospermopsis raciborskii, Raphidiopsis brookii, Coleofasciculus chthonoplastes, Cyanobium sp., Nodularia spumigena, Spirulina subsalsa, Prochlorococcus marinus subsp. marinus, Prochlorococcus marinus subsp. pastoris, 'Nostoc azollae' 0708, Candidatus Atelocyanobacterium thalassa (D3EMX6), Lyngbya aestuarii (Q2EJS7), Nostoc sp. (Q3C1T8), Nostoc sp. (Q8YZ11), Lyngbya majuscula (Q846P6), Trichormus variabilis (Q9ZAK2), Cylindrospermopsis raciborskii CS-505, Crocosphaera subtropica ATCC 51142 CCY 0110, Arthrospira platensis FACHB341, Arthrospira platensis Paraca, Prochlorococcus marinus MIT 9313, Lyngbya majuscula CCAP 1446/4 (Q846P6), Crocosphaera subtropica ATCC 51142 PCC 8802, Synechococcus sp. PCC 7335, Prochlorococcus marinus subsp. marinus CCMP1375, Spirulina subsalsa FACHB351, Prochlorococcus marinus subsp. pastoris CCMP1986, Prochlorococcus marinus MIT 9301, Prochlorococcus marinus MIT 9303, Gloeocapsa alpicola CALU 743, Arthrospira platensis FACHB439, Prochlorococcus marinus MIT 9211, Prochlorococcus marinus NATL1A, Lyngbya aestuarii CCY 9616 (Q2EJS7), Prochlorococcus marinus MIT 9312, Synechococcus sp. BL107, Crocosphaera subtropica ATCC 51142 PCC 7424, Synechococcus sp. CC9311, Cyanobium sp. PCC 7001, Raphidiopsis brookii D9, Crocosphaera watsonii WH 8501, Synechococcus sp. WH 7803, Synechococcus sp. JA-3-3Ab, Crocosphaera subtropica ATCC 51142 PCC 8801, Limnospira maxima CS-328, Synechococcus sp. RCC307, Nostoc sp. PCC 7120 (Q8YZ11), Nostoc sp. PCC 7422 (Q3C1T8), Synechococcus sp. CC9902, Arthrospira platensis FACHB791, Prochlorococcus marinus MIT 9215, Crocosphaera subtropica ATCC 51142 PCC 7822, Prochlorococcus marinus NATL2A, Cronbergia siamensis SAG 11.82 TISTR 8012, Synechococcus elongatus PCC 7942, Crocosphaera subtropica ATCC 51142 PCC 7425b, Arthrospira platensis FACHB440, Prochlorococcus marinus AS9601, Prochlorococcus marinus MIT 9515, Limnospira maxima FACHBSM, Arthrospira platensis FACHBOUQDS6, Coleofasciculus chthonoplastes PCC 7420, Nodularia spumigena CCY 9414, Microcystis aeruginosa NIES-843, Synechococcus sp. WH 8102
Cracknell, J.A.; Wait, A.F.; Lenz, O.; Friedrich, B.; Armstrong, F.A.
A kinetic and thermodynamic understanding of O2 tolerance in [NiFe]-hydrogenases
Proc. Natl. Acad. Sci. USA
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Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Sasaki, D.; Watanabe, S.; Kanai, T.; Atomi, H.; Imanaka, T.; Miki, K.
Characterization and in vitro interaction study of a [NiFe] hydrogenase large subunit from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1
Biochem. Biophys. Res. Commun.
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Thermococcus kodakarensis (Q8NKS2)
Camsund, D.; Devine, E.; Holmqvist, M.; Yohanoun, P.; Lindblad, P.; Stensjoe, K.
A HupS-GFP fusion protein demonstrates a heterocyst-specific localization of the uptake hydrogenase in Nostoc punctiforme
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Nostoc punctiforme, Nostoc punctiforme ATCC 29133
Abou Hamdan, A.; Dementin, S.; Liebgott, P.P.; Gutierrez-Sanz, O.; Richaud, P.; De Lacey, A.L.; Rousset, M.; Bertrand, P.; Cournac, L.; Leger, C.
Understanding and tuning the catalytic bias of hydrogenase
J. Am. Chem. Soc.
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Solidesulfovibrio fructosivorans
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Role of the NiFe hydrogenase Hya in oxidative stress defense in Geobacter sulfurreducens
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Geobacter sulfurreducens
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Kinetic modeling of hydrogen conversion at [Fe] hydrogenase active-site models
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synthetic construct
Pinske, C.; McDowall, J.S.; Sargent, F.; Sawers, R.G.
Analysis of hydrogenase 1 levels reveals an intimate link between carbon and hydrogen metabolism in Escherichia coli K-12
Microbiology
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Escherichia coli (P0ACD8), Escherichia coli (P69739), Escherichia coli
Fritsch, J.; Scheerer, P.; Frielingsdorf, S.; Kroschinsky, S.; Friedrich, B.; Lenz, O.; Spahn, C.
The crystal structure of an oxygen-tolerant hydrogenase uncovers a novel iron-sulphur centre
Nature
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Cupriavidus necator (P31891), Cupriavidus necator DSM 428 (P31891)
Rippers, Y.; Utesch, T.; Hildebrandt, P.; Zebger, I.; Mroginski, M.A.
Insights into the structure of the active site of the O2-tolerant membrane bound [NiFe] hydrogenase of R. eutropha H16 by molecular modelling
Phys. Chem. Chem. Phys.
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Cupriavidus necator (P31891), Cupriavidus necator DSM 428 (P31891)
Volbeda, A.; Darnault, C.; Parkin, A.; Sargent, F.; Armstrong, F.A.; Fontecilla-Camps, J.C.
Crystal structure of the O(2)-tolerant membrane-bound hydrogenase 1 from Escherichia coli in complex with its cognate cytochrome b
Structure
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Escherichia coli (P69739), Escherichia coli
Hei, D.J.; Clark, D.S.
Pressure stabilization of proteins from extreme thermophiles
Appl. Environ. Microbiol.
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Methanocaldococcus jannaschii, Methanococcus maripaludis, Methanothermococcus thermolithotrophicus, Methanotorris igneus
Flanagan, L.A.; Wright, J.J.; Roessler, M.M.; Moir, J.W.; Parkin, A.
Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance
Chem. Commun. (Camb.)
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Escherichia coli
Filippi, G.; Arrigoni, F.; Bertini, L.; De Gioia, L.; Zampella, G.
DFT dissection of the reduction step in H2 catalytic production by [FeFe]-hydrogenase-inspired models can the bridging hydride become more reactive than the terminal isomer?
Inorg. Chem.
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Clostridium pasteurianum
Pinske, C.; Jaroschinsky, M.; Linek, S.; Kelly, C.L.; Sargent, F.; Sawers, R.G.
Physiology and bioenergetics of [NiFe]-hydrogenase 2-catalyzed H2-consuming and H2-producing reactions in Escherichia coli
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Escherichia coli
Chandrayan, S.K.; Wu, C.H.; McTernan, P.M.; Adams, M.W.
High yield purification of a tagged cytoplasmic [NiFe]-hydrogenase and a catalytically-active nickel-free intermediate form
Protein Expr. Purif.
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90-94
2015
Pyrococcus furiosus, Pyrococcus furiosus MW0430
Yang, C.; Li, Z.; Zhao, D.; Chen, J.; Zhu, X.; Zhang, X.; Bi, C.
Engineering an efficient H2 utilizing Escherichia coli platform by modulation of endogenous hydrogenases
Biochem. Eng. J.
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107851
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Escherichia coli, Escherichia coli ATCC 8739
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Beaton, S.E.; Evans, R.M.; Finney, A.J.; Lamont, C.M.; Armstrong, F.A.; Sargent, F.; Carr, S.B.
The structure of hydrogenase-2 from Escherichia coli implications for H2-driven proton pumping
Biochem. J.
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Escherichia coli (P69741), Escherichia coli K12 (P69741)
Hartmann, S.; Frielingsdorf, S.; Ciaccafava, A.; Lorent, C.; Fritsch, J.; Siebert, E.; Priebe, J.; Haumann, M.; Zebger, I.; Lenz, O.
O2-tolerant H2 activation by an isolated large subunit of a [NiFe] hydrogenase
Biochemistry
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5339-5349
2018
Cupriavidus necator, Cupriavidus necator HF795
Loeffler, M.; Kuemmel, S.; Vogt, C.; Richnow, H.H.
H2 kinetic isotope fractionation superimposed by equilibrium isotope fractionation during hydrogenase activity of D. vulgaris strain Miyazaki
Front. Microbiol.
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1545
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Desulfovibrio vulgaris, Desulfovibrio vulgaris DSM 19637, Desulfovibrio vulgaris Miyazaki F
Mirzoyan, S.; Trchounian, A.; Trchounian, K.
Role of hydrogenases 3 and 4 in Escherichia coli growth and H2 producing hydrogenase activity during anaerobic utilization of lactose
Int. J. Hydrogen Energy
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2018
Escherichia coli, Escherichia coli BW25113, Escherichia coli MC 4100
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Zhang, L.; Morello, G.; Carr, S.B.; Armstrong, F.A.
Aerobic photocatalytic H2 production by a [NiFe] hydrogenase engineered to place a silver nanocluster in the electron relay
J. Am. Chem. Soc.
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2020
Escherichia coli (P0ACD8), Escherichia coli, Escherichia coli K12 (P0ACD8)
Eckert, C.A.; Freed, E.; Wawrousek, K.; Smolinski, S.; Yu, J.; Maness, P.C.
Inactivation of the uptake hydrogenase in the purple non-sulfur photosynthetic bacterium Rubrivivax gelatinosus CBS enables a biological water-gas shift platform for H2 production
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46
993-1002
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Rubrivivax gelatinosus, Rubrivivax gelatinosus CBS
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