We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments A hemoprotein. A manganese protein containing MnIII in the resting state, which also belongs here, is often called pseudocatalase. The enzymes from some organisms, such as Penicillium simplicissimum, can also act as a peroxidase (EC 1.11.1.7) for which several organic substances, especially ethanol, can act as a hydrogen donor. Enzymes that exhibit both catalase and peroxidase activity belong under EC 1.11.1.21, catalase-peroxidase.
The taxonomic range for the selected organisms is: Neurospora crassa The enzyme appears in selected viruses and cellular organisms
Synonyms
catalase, cat-1, catalase a, manganese catalase, polyethylene glycol-catalase, catalase-1, cat-a, catpo, catalase p, haem catalase,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
catalase-peroxidase
-
-
-
-
polyethylene glycol-catalase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
hydrogen-peroxide:hydrogen-peroxide oxidoreductase
A hemoprotein. A manganese protein containing MnIII in the resting state, which also belongs here, is often called pseudocatalase. The enzymes from some organisms, such as Penicillium simplicissimum, can also act as a peroxidase (EC 1.11.1.7) for which several organic substances, especially ethanol, can act as a hydrogen donor. Enzymes that exhibit both catalase and peroxidase activity belong under EC 1.11.1.21, catalase-peroxidase.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3,3'-diaminobenzidine + H2O2
? + H2O
isoform Cat-2
-
?
ascorbate + H2O2
? + H2O
isoform Cat-2
-
?
o-dianisidine + H2O2
? + H2O
isoform Cat-2
-
?
o-methoxyphenol + H2O2
? + H2O
H2O2
O2 + H2O
-
-
-
?
H2O2
O2 + H2O
-
no peroxidase activity
-
?
H2O2
O2 + H2O
two monofunctional catalases, Cat-1 and Cat-3, one catalase-peroxidase enzyme, Cat-2
-
?
o-methoxyphenol + H2O2
? + H2O
isoform Cat-2
-
?
o-methoxyphenol + H2O2
? + H2O
i.e. guaiacol
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
H2O2
O2 + H2O
-
-
-
?
H2O2
O2 + H2O
-
no peroxidase activity
-
?
H2O2
O2 + H2O
two monofunctional catalases, Cat-1 and Cat-3, one catalase-peroxidase enzyme, Cat-2
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
heme
CAT-1 tetramer includes 3450 water molecules and four heme groups
heme
-
ferric heme
heme
all isoforms have chlorine instead of ferro-protoheme IX at the active site
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Cu2+
-
less than 0.2 g-atom cupper per mol enzyme
Iron
-
3.4 g-atom iron per mol enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3-amino-1H-1,2,4-triazole
50% inactivation in 1 h
hydroxyl radicals
inactivation of isoform Cat-1
KNO2
-
37% inhibition at 1 mM
KNO3
-
14% inhibition at 1 mM
NaN3
-
98% inhibition at 1 mM
KCN
-
97% inhibition at 1 mM
KCN
isoform Cat-2, IC50: 0.146 mM, catalase activity, IC50: 0.168 mM, peroxidase activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.025
H2O2
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
125000
H2O2
isoform Cat-2
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.168
KCN
Neurospora crassa
isoform Cat-2, IC50: 0.146 mM, catalase activity, IC50: 0.168 mM, peroxidase activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
130
isoform Cat-2, peroxidase activity with 3,3-diaminobenzidine
1300
isoform Cat-2, peroxidase activity with guaiacol
1600
isoform Cat-2, peroxidase activity with o-dianisidine
470
isoform Cat-2, peroxidase activity with ascorbate
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4.75
isoform Cat-2, peroxidase activity
6.25
isoform Cat-2, catalase activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
brenda
isoform Cat-1 mainly present
brenda
isoform Cat-3 mainly present
brenda
-
inducible by nitrate containing growth medium
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
isoform Cat-3 secreted
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
320000
-
sedimentation equilibrium analysis, gel filtration
85000
-
4 * 85000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
homotetramer
x-ray crystallography
monomer
1 * 80000, isoforms Cat-1, Cat-3
tetramer
-
4 * 85000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
glycoprotein
-
2-4% carbohydrate content
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4
-
inactivation below
439803
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
100
isoform Cat-1, half-life: 70 min
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
isoform Cat-1, very stable for months, resistant to denaturizing agents such as urea or guanidine-HCl
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
oxidation by singlet oxygen
439772
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
isoforms Cat-1, Cat-2, Cat-3
to homogeneity, chromatography techniques
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Peraza, L.; Hansberg, W.
Neurospora crassa catalases, singlet oxygen and cell differentiation
Biol. Chem.
383
569-575
2002
Aspergillus nidulans, Neurospora crassa, Neurospora crassa (Q8X182)
brenda
Jacob, G.S.; Orme-Johnson, W.H.
Catalase of Neurospora crassa. 1. Induction, purification, and physical properties
Biochemistry
18
2967-2974
1979
Neurospora crassa
brenda
Jacob, G.S.; Orme-Johnson, W.H.
Catalase of Neurospora crassa. 2. Electron paramagnetic resonance and chemical properties of the prosthetic group
Biochemistry
18
2975-2980
1979
Neurospora crassa
brenda
Dominguez, L.; Sosa-Peinado, A.; Hansberg, W.
Catalase evolved to concentrate H2O2 at its active site
Arch. Biochem. Biophys.
500
82-91
2010
Neurospora crassa (Q9C168), Neurospora crassa
brenda