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Information on EC 1.11.1.12 - phospholipid-hydroperoxide glutathione peroxidase and Organism(s) Bos taurus and UniProt Accession Q9N2J2

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IUBMB Comments
A protein containing a selenocysteine residue. The products of action of EC 1.13.11.12 lipoxygenase on phospholipids can act as acceptors; H2O2 can also act, but much more slowly (cf. EC 1.11.1.9 glutathione peroxidase).
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This record set is specific for:
Bos taurus
UNIPROT: Q9N2J2
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The taxonomic range for the selected organisms is: Bos taurus
The enzyme appears in selected viruses and cellular organisms
Synonyms
selenoprotein p, phgpx, glutathione peroxidase 4, phospholipid hydroperoxide glutathione peroxidase, gpx-4, glutathione peroxidase-4, npgpx, selenoperoxidase, gpx4b, gpx4a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospholipid hydroperoxide glutathione peroxidase
-
GPx4
-
-
hydroperoxide glutathione peroxidase
-
-
-
-
peroxidation-inhibiting protein
-
-
-
-
peroxidation-inhibiting protein: peroxidase, glutathione (phospholipid hydroperoxide-reducing)
-
-
-
-
PHGPX
phospholipid hydroperoxide glutathione peroxidase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
reduction
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
glutathione:lipid-hydroperoxide oxidoreductase
A protein containing a selenocysteine residue. The products of action of EC 1.13.11.12 lipoxygenase on phospholipids can act as acceptors; H2O2 can also act, but much more slowly (cf. EC 1.11.1.9 glutathione peroxidase).
CAS REGISTRY NUMBER
COMMENTARY hide
97089-70-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glutathione + L-alpha-phosphatidylcholine hydroperoxide
?
show the reaction diagram
-
-
-
?
glutathione + a lipid hydroperoxide
glutathione disulfide + lipid + H2O
show the reaction diagram
-
-
-
-
?
glutathione + cholesterol hydroperoxides
?
show the reaction diagram
-
-
-
-
?
glutathione + cumene hydroperoxide
?
show the reaction diagram
-
no activity
-
-
?
glutathione + H2O2
?
show the reaction diagram
glutathione + lipid hydroperoxide
glutathione disulfide + lipid + H2O
show the reaction diagram
glutathione + phosphatidylcholine hydroperoxide
?
show the reaction diagram
-
activity assay
-
-
?
glutathione + phospholipid hydroperoxide
oxidized glutathione + phospholipid + H2O
show the reaction diagram
-
-
-
-
r
glutathione + phospholipid hydroperoxides
?
show the reaction diagram
-
-
-
-
?
glutathione + tert-butyl hydroperoxide
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glutathione + a lipid hydroperoxide
glutathione disulfide + lipid + H2O
show the reaction diagram
-
-
-
-
?
glutathione + lipid hydroperoxide
glutathione disulfide + lipid + H2O
show the reaction diagram
-
protection of biomembranes against oxidative damage
-
-
?
additional information
?
-
-
enzyme inhibits lipid peroxidation, which is induced by Fe3+-triethylenetetramine complex, NADPH and ascorbate in presence of glutathione, therefore a physiological amount of alpha-tocopherol, vitamin E, is necessary in the membranes
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glutathione
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
selenium
-
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha-tocopherol
-
complete inhibition of glutathione oxidation
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
17-beta-estradiol
causes increase in GPx-4 activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.37
glutathione
-
-
0.011
phospholipid hydroperoxide
-
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.019
alpha-tocopherol
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.04
-
low-PHGPx group
0.0539
-
low-PHGPx, Chianina
0.056
-
low-PHGPx, Marchigiana
0.0746
-
low-PHGPx, Romagnola
0.13
-
high-PHGPx group
0.2242
-
high-PHGPx, Chianina
0.2447
-
high-PHGPx, Romagnola
0.2453
-
high-PHGPx, Marchigiana
7
-
purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
assay at
7.5
-
activity assay
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23
-
activity assay at room temperature
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
-
-
Manually annotated by BRENDA team
-
high-salt extract from retina
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GPX4_BOVIN
197
0
22240
Swiss-Prot
Secretory Pathway (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20000
-
-
6000
-
below, retina enzyme, gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
PHGPx expression is up-regulated in spermatozoa by unknown mechanisms
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
an assay for sperm PHGPx activity emerges as a unique tool to evaluate semen quality for sire selection
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Maiorino, M.; Gregolin, C.; Ursini, F.
Phospholipid hydroperoxide glutathione peroxidase
Methods Enzymol.
186
448-457
1990
Bos taurus, Canis lupus familiaris, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Lam, K.W.; Wang, L.; Hong, B.S.; Treble, D.
Purification of phospholipid hydroperoxide glutathione peroxidase from bovine retina
Curr. Eye Res.
12
9-15
1993
Bos taurus
Manually annotated by BRENDA team
Lapointe, J.; Kimmins, S.; Maclaren, L.A.; Bilodeau, J.F.
Estrogen selectively up-regulates the phospholipid hydroperoxide glutathione peroxidase in the oviducts
Endocrinology
146
2583-2592
2005
Bos taurus (Q9N2J2), Bos taurus
Manually annotated by BRENDA team
Stradaioli, G.; Sylla, L.; Monaci, M.; Maiorino, M.
Phospholipid hydroperoxide glutathione peroxidase in bull spermatozoa provides a unique marker in the quest for semen quality analysis
Theriogenology
72
91-98
2009
Bos taurus
Manually annotated by BRENDA team