Information on EC 1.10.3.1 - catechol oxidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.10.3.1
-
RECOMMENDED NAME
GeneOntology No.
catechol oxidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 catechol + O2 = 2 1,2-benzoquinone + 2 H2O
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
-
Isoquinoline alkaloid biosynthesis
-
-
Metabolic pathways
-
-
o-diquinones biosynthesis
-
-
Tyrosine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
1,2-benzenediol:oxygen oxidoreductase
A type 3 copper protein that catalyses exclusively the oxidation of catechol (i.e., o-diphenol) to the corresponding o-quinone. The enzyme also acts on a variety of substituted catechols. It is different from tyrosinase, EC 1.14.18.1, which can catalyse both the monooxygenation of monophenols and the oxidation of catechols.
CAS REGISTRY NUMBER
COMMENTARY hide
9002-10-2
not distinguished from EC 1.14.18.1
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain A-2
-
-
Manually annotated by BRENDA team
pawpaw fruit
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
cv. Conilon
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Coffea guarini
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
two different varieties Cucumis melo L. cantalupensis cv. Charentais and Cucumis melo L. inodorus cv. Amarillo. Polyphenol oxidase (PPO) and peroxidase (POD) are extracted from two different varieties of melon
-
-
Manually annotated by BRENDA team
var. Gigante de Vranja, quince
-
-
Manually annotated by BRENDA team
enzyme may be involved in photosynthesis, flower coloration and plant disease resistance
-
-
Manually annotated by BRENDA team
hair crab
-
-
Manually annotated by BRENDA team
cv. Algerie
-
-
Manually annotated by BRENDA team
Ferula sp.
-
-
-
Manually annotated by BRENDA team
cv. Elsanta
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
variety Yate
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Fuji apple
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Mycelia sterilia
IBR 35219/2
-
-
Manually annotated by BRENDA team
Mycelia sterilia IBR 35219/2
IBR 35219/2
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
from different places in Turkey
-
-
Manually annotated by BRENDA team
spiny lobster
-
-
Manually annotated by BRENDA team
isoenzymes Ia, Ib and II
-
-
Manually annotated by BRENDA team
mamey
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
peach
-
-
Manually annotated by BRENDA team
gene RSc0337
-
-
Manually annotated by BRENDA team
mediterranean slipper lobster
-
-
Manually annotated by BRENDA team
var. Naomi, Pizzutello, Rosa Maletto, and PO228, physicochemical properties of the different tomato varieties, overview
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-
Manually annotated by BRENDA team
brinjal
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-
Manually annotated by BRENDA team
var. Jasim, purple-flesh potato
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-
Manually annotated by BRENDA team
laccase-like secreted enzyme
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-
Manually annotated by BRENDA team
PPO-6; gene ppo-6
UniProt
Manually annotated by BRENDA team
gene tyr2
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-
Manually annotated by BRENDA team
two isozymes
-
-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
AoCO4 belongs to the short-tyrosinase family. The catalytic differences to the phenolases, EC 1.14.18.1, are not due to structural features
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-catechin + O2
?
show the reaction diagram
(+)-catechin hydrate + O2
?
show the reaction diagram
-
-
-
-
?
(-)-catechin + O2
?
show the reaction diagram
-
enzyme activity relative to 4-methylcatechol: 0.77%
-
-
?
(-)-epicatechin + O2
?
show the reaction diagram
(-)-epigallocatechin + O2
?
show the reaction diagram
(-)-epigallocatechin gallate + O2
?
show the reaction diagram
(R)-dopaxanthin + dehydroascorbic acid + O2
(R)-dopaxanthin quinone + L-ascorbic acid + H2O
show the reaction diagram
-
(R)-dopaxanthin is a pigment, the reaction rate on the (R)-isomer of dopaxanthin is 1.9fold lower than that for the (S)-isomer
quantitative product analysis
-
?
(R)-tyrosine-betaxanthin + L-DOPA + O2
(R)-dopaxanthin + dopaquinone + H2O
show the reaction diagram
-
i.e. (R)-portulacaxanthin II, the activity of the enzyme is not restricted to betaxanthins derived from (S)-amino acids
( R)-dopaxanthin is a pigment, quantitative product analysis
-
?
1-butylcatechol + O2
?
show the reaction diagram
-
-
-
-
?
1-tert-butyl-catechol + O2
?
show the reaction diagram
-
-
-
-
?
2 4-methylcatechol + O2
2 4-methyl-1,2-benzoquinone + 2 H2O
show the reaction diagram
2 catechol + O2
2 1,2-benzoquinone + 2 H2O
show the reaction diagram
2 dopamine + O2
2 4-(2-aminoethyl)-1,2-benzoquinone + 2 H2O
show the reaction diagram
2 o-diphenol + O2
2 o-quinone + 2 H2O
show the reaction diagram
2,3,4-trihydroxybenzoic acid + O2
?
show the reaction diagram
2,3-dihydroxybenzoic acid + O2
?
show the reaction diagram
-
-
-
-
?
2-methylhydroquinone + O2
?
show the reaction diagram
-
low activity
-
-
?
3,4,5-trihydroxy-L-phenylalanine + O2
?
show the reaction diagram
3,4,5-trihydroxybenzoic acid + O2
?
show the reaction diagram
3,4-dihydroxyhydrocinnamic acid + O2
?
show the reaction diagram
-
-
-
-
?
3,4-dihydroxyphenyl acetic acid + O2
?
show the reaction diagram
3,4-dihydroxyphenyl propionic acid + O2
?
show the reaction diagram
-
-
-
-
?
3,4-dihydroxyphenylacetic acid + O2
(3,4-dioxocyclohexa-1,5-dien-1-yl)acetic acid + H2O
show the reaction diagram
3,4-dihydroxyphenylacetic acid + O2
?
show the reaction diagram
-
-
-
-
?
3,5-di-tert-butylcatechol + O2
3,5-di-tert-butyl-o-benzoquinone + H2O
show the reaction diagram
-
mechanism, the rate-determining step is found to change with the substrate to complex ratio, I2+ reacts with DTBCH2, while undergoing a one-electron reduction, leading to the formation of mixed-valence CuIICuI–semiquinone species DTSQ
-
-
?
3-(3,4-dihydroxyphenyl)-L-alanine + O2
?
show the reaction diagram
-
enzyme activity relative to 4-methylcatechol: 1.15%
-
-
?
4-methylcatechol + O2
4-methyl-1,2-benzoquinone
show the reaction diagram
4-methylcatechol + O2
4-methyl-1,2-benzoquinone + H2O
show the reaction diagram
4-methylcatechol + O2
4-methyl-o-benzoquinone
show the reaction diagram
4-methylcatechol + O2
?
show the reaction diagram
4-O-caffeoylquinic acid + O2
?
show the reaction diagram
-
-
-
-
?
4-tert-butylcatechol + O2
4-tert-butyl-1,2-benzoquinone + H2O
show the reaction diagram
4-tert-butylcatechol + O2
?
show the reaction diagram
4-[(4-methylbenzol)azo]-1,2-benzenediol + O2
?
show the reaction diagram
-
-
-
-
?
5-caffeoyl quinic acid + O2
?
show the reaction diagram
aminophenol + O2
?
show the reaction diagram
-
-
-
-
?
aniline + O2
?
show the reaction diagram
-
enzyme shows also activity after 24 h incubation
-
-
?
betanidin + O2
betanidin-quinone + H2O
show the reaction diagram
-
the structural unit of the violet betacyanins from Lampranthus productus flowers, the reacion is reversible by ascorbic acid addition at pH 5.0 and 4°C
-
-
?
caffeic acid + 1/2 O2
caffeoyl quinone + H2O
show the reaction diagram
caffeic acid + O2
?
show the reaction diagram
caffeic acid + O2
caffeoyl quinone + H2O
show the reaction diagram
catechin + O2
?
show the reaction diagram
catechol + 1/2 O2
1,2-benzoquinone + H2O
show the reaction diagram
catechol + O2
1,2-benzoquinone + H2O
show the reaction diagram
catechol + O2
?
show the reaction diagram
catechol + O2
o-benzoquinone + H2O
show the reaction diagram
-
-
-
-
?
chlorogenic acid + O2
?
show the reaction diagram
coumaric acid + O2
?
show the reaction diagram
cumaric acid + O2
?
show the reaction diagram
-
-
-
-
?
D-catechin + O2
?
show the reaction diagram
D-dopa + 1/2 O2
D-dopaquinone + H2O
show the reaction diagram
dicatechol + O2
?
show the reaction diagram
digallol + O2
?
show the reaction diagram
dihydrocaffeic acid + O2
?
show the reaction diagram
-
-
-
-
?
dihydroxyphenylalanine + O2
dihydroxyphenylalanine quinone + H2O
show the reaction diagram
-
-
-
-
?
DL-2-methyl-3,4-dihydroxyphenylalanine + O2
2-methyldopaquinone + H2O
show the reaction diagram
-
the L-isomer is preferred
-
-
?
DL-DOPA + O2
dopaquinone + H2O
show the reaction diagram
DL-isoproterenol + O2
?
show the reaction diagram
dopamine + 1/2 O2
dopamine quinone + H2O
show the reaction diagram
-
-
-
-
?
dopamine + O2
4-(2-aminoethyl)-1,2-benzoquinone + 2 H2O
show the reaction diagram
dopamine + O2
?
show the reaction diagram
dopamine + O2
dopaminequinone + H2O
show the reaction diagram
-
-
-
-
?
epicatechin + O2
?
show the reaction diagram
epinephrine + O2
?
show the reaction diagram
ferulic acid + O2
?
show the reaction diagram
-
-
-
-
?
gallic acid + O2
?
show the reaction diagram
guaiacol + O2
?
show the reaction diagram
-
-
-
-
?
hydroquinone + O2
?
show the reaction diagram
-
low activity
-
-
?
hydroxyquinone + O2
?
show the reaction diagram
L-3,4-dihydroxyphenylalanine methyl ester + 1/2 O2
L-dopaquinone methyl ester + H2O
show the reaction diagram
-
-
-
-
?
L-3-hydroxytyrosine + L-dopa + O2
?
show the reaction diagram
L-adrenaline + O2
?
show the reaction diagram
-
-
-
-
?
L-catechin + O2
?
show the reaction diagram
-
-
-
-
?
L-dihydroxyphenylalanine + O2
L-dopaquinone + H2O
show the reaction diagram
-
L-dopa
-
-
?
L-dopa + 1/2 O2
L-dopaquinone + H2O
show the reaction diagram
L-DOPA + O2
?
show the reaction diagram
L-dopa + O2
dopachrome + H2O
show the reaction diagram
L-DOPA + O2
dopaquinone + H2O
show the reaction diagram
L-Dopa + O2
L-dopaquinone + H2O
show the reaction diagram
L-noradrenaline + O2
?
show the reaction diagram
-
-
-
-
?
L-tyrosine + L-dopa + O2
L-dopa + dopaquinone + H2O
show the reaction diagram
L-tyrosine + O2
dihydroxyphenylalanine + H2O
show the reaction diagram
-
-
-
-
?
Luteolin-7-glycoside + O2
?
show the reaction diagram
-
-
-
-
?
myricetin + O2
?
show the reaction diagram
-
-
-
-
?
N-acetyldopamine + O2
?
show the reaction diagram
-
NADA
-
-
?
Norepinephrine + O2
?
show the reaction diagram
-
-
-
-
?
p-cresol + O2
?
show the reaction diagram
-
enzyme shows also activity after 24 h incubation
-
-
?
p-hydroquinone + O2
?
show the reaction diagram
phenol + O2
?
show the reaction diagram
-
enzyme shows also activity after 24 h incubation
-
-
?
phloroglucinol + O2
?
show the reaction diagram
-
-
-
-
?
protocatechuic acid + O2
?
show the reaction diagram
pyrogallic acid + O2
?
show the reaction diagram
-
-
-
-
?
pyrogallol + O2
?
show the reaction diagram
quercetin + O2
?
show the reaction diagram
rosmarinic acid + O2
?
show the reaction diagram
-
-
-
-
?
rutin + O2
?
show the reaction diagram
-
-
-
-
?
shikimic acid + O2
?
show the reaction diagram
-
-
-
-
?
tannic acid + O2
?
show the reaction diagram
-
-
-
-
?
tert-butyl-catechol + O2
?
show the reaction diagram
-
2fold faster reaction rate with the particulate, latent enzyme form compared to the soluble active enzyme
-
-
?
tert-butylcatechol + O2
?
show the reaction diagram
tetramethylbenzidine + O2
?
show the reaction diagram
-
enzyme shows low affinity to this substrate
-
-
?
tyrosol + O2
?
show the reaction diagram
-
enzyme shows also activity after 24 h incubation
-
-
?
verbascosid + O2
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(+)-catechin + O2
?
show the reaction diagram
Ferula sp.
-
-
-
-
?
(-)-epicatechin + O2
?
show the reaction diagram
Ferula sp.
-
-
-
-
?
2 4-methylcatechol + O2
2 4-methyl-1,2-benzoquinone + 2 H2O
show the reaction diagram
-
-
-
-
?
2 catechol + O2
2 1,2-benzoquinone + 2 H2O
show the reaction diagram
-
-
-
-
?
2 o-diphenol + O2
2 o-quinone + 2 H2O
show the reaction diagram
I7HUF2
-
-
-
?
3,4,5-trihydroxy-L-phenylalanine + O2
?
show the reaction diagram
-
cytotoxicity of TOPA
-
-
?
4-methylcatechol + O2
4-methyl-1,2-benzoquinone + H2O
show the reaction diagram
Ferula sp.
-
-
-
-
?
catechol + 1/2 O2
1,2-benzoquinone + H2O
show the reaction diagram
chlorogenic acid + O2
?
show the reaction diagram
D-catechin + O2
?
show the reaction diagram
-
-
-
-
?
dihydroxyphenylalanine + O2
dihydroxyphenylalanine quinone + H2O
show the reaction diagram
-
-
-
-
?
dopamine + O2
?
show the reaction diagram
-
-
-
-
?
L-dopa + 1/2 O2
L-dopaquinone + H2O
show the reaction diagram
L-tyrosine + L-dopa + O2
L-dopa + dopaquinone + H2O
show the reaction diagram
L-tyrosine + O2
dihydroxyphenylalanine + H2O
show the reaction diagram
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
1 mM, slight stimulation
copper
Cu+
-
1 mM, 234% activity
Hg2+
-
activates
Ni2+
-
activates
Pb2+
-
1 mM, slight stimulation
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(-)-epigallocatechin
(-)-epigallocatechin-3-O-gallate
(R)-HTCCA
(S)-HTCCA
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide
-
irreversible inactivation, second-order rate constants
1-Phenyl-2-thiourea
2,3-Dihydroxybenzoic acid
2,4-dihydroxy-N-(3,4,5-trihydroxybenzyl)benzamide
-
IC50: 0.550 mM
2,4-dihydroxy-N-(4-hydroxybenzyl)benzamide
-
IC50: 1.820 mM
2-hydroxy-2,4,6-cycloheptatrien-1-one
2-mercaptobenzothiazole
-
1 mM, 94% inhibition
2-mercaptoethanol
2-methyl-4-[(E)-(4-nitrophenyl)methylidene]-1,3-oxazol-5(4H)-one
-
IC50: 0.00351 mM
2-methyl-4-[(E)-2-thienylmethylidene]-1,3-oxazol-5-one
-
IC50: 0.00311 mM
2-methyl-4-[(E,2Z)-3-phenyl-2-propenyliden]-1,3-oxazol-5(4H)-one
-
IC50: 0.00123 mM
3,4,5-trihydroxy-N-(3,4,5-trihydroxybenzyl)benzamide
-
IC50: 0.555 mM
3,4,5-trihydroxy-N-(4-hydroxybenzyl)benzamide
-
IC50: 1.180 mM
3,4,5-Trihydroxybenzoic acid
3,4-dihydroxy-N-(3,4,5-trihydroxybenzyl)benzamide
-
IC50: 0.280 mM
3,4-dihydroxy-N-(4-hydroxybenzyl)benzamide
-
IC50: 2.0 mM
3,4-dihydroxybenzoic acid
3,5-dihydroxy-N-(3,4,5-trihydroxybenzyl)benzamide
-
IC50: 0.705 mM
3,5-dihydroxy-N-(4-hydroxybenzyl)benzamide
-
IC50: 0.710 mM
3-(acetoyloxy)-2-hydroxy-4-[[5-oxo-2-phenyl-1,3-oxazol-4(5H)-ylidene]methyl]phenylacetate
-
IC50: 0.00215 mM
3-aminophenyl-2,2'-methylenebis-(5,5-dimethylcyclohexane-1,3-dione)
-
IC50: 0.0021 mM
3-aminophenyl-2,2'-methylenebis-(cyclohexane-1,3-dione)
-
IC50: 0.00219 mM
3-chlorophenyl-2,2'-methylenebis-(5,5-dimethylcyclohexane-1,3-dione)
-
IC50: 0.0032 mM
4-chloromercuribenzoate
-
1 mM, 96, 94 and 95% inhibition of isoenzymes Ia, Ib and II respectively
4-hexylresorcinol
4-hydroxybenzoic acid
-
-
4-Methylcatechol
-
field bean PPO obeys Michaelis-Menten kinetics and exhibits the phenomenon of inhibition by excess substrate for catechol, 4-methylcatechol and 4-tert-butylcatechol
4-tert-butylcatechol
-
field bean PPO obeys Michaelis-Menten kinetics and exhibits the phenomenon of inhibition by excess substrate for catechol, 4-methylcatechol and 4-tert-butylcatechol
4-[(E)-(4-nitrophenyl)methylidene]-2-phenyl-1,3-oxazol-5(4H)-one
-
IC50: 0.00323 mM
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
8-hydroxyquinoline
-
-
Ag+
-
1 mM, 60% inhibition
Al3+
-
1 mM, 89% inhibition
aloesin
Anisaldehyde
ascorbate
-
-
ascorbic acid
askendoside B
-
; IC50 : 0.014 mM
askendoside D
-
-
azelaic acid
azide
-
typical inhibitors of catecholoxidase, also inhibit the phenoloxidase activity of activated hemocyanin
Ba2+
-
moderately inhibits PPO
benzaldehyde
-
-
Benzoic acid
beta-mercaptoethanol
-
-
Boric acid
-
-
bryoamaride
-
-
captopril
catechol
cinnamaldehyde
Cinnamic acid
-
-
citral
-
noncompetitive inhibitor
Citric acid
cucurbitane glycosides
-
isolated from Bryonia, structure–activity relationships, overview
-
cuminaldehyde
Cupferron
Cuprizone
-
-
cycloartane glycosides
-
isolated from Astragalus sp., structure–activity relationships, overview
-
cyclocarposide
-
-
cycloorbicoside G
-
-
cyclosieversioside F
-
-
cysteine
D-fructose
-
D-fructose at different concentrations, PPO activities are measured at 25°C and pH 7.0 to determine inhibitor effects of sugars on enzymatic activities. PPO activities from both cultivars show a decreasing pattern as sugar concentration in the assay medium increases
D-glucose
-
D-glucose at different concentrations, PPO activities are measured at 25°C and pH 7.0 to determine inhibitor effects of sugars on enzymatic activities. PPO activities from both cultivars show a decreasing pattern as sugar concentration in the assay medium increases
decahydro-2-naphthyl gallate
diethyldithiocarbamate
diethyldithiocarbamic acid
-
-
dithiothreitol
DL-dithiothreitol
-
competitive with 4-methylcatechol, catechol or pyrogallol. IC50: 0.147 mM in reaction with 4-methylcatechol, IC50: 0.0329 mM in reaction with pyrogallol, IC50: 0.135 mM in reaction with catechol
dopamine
dopastin
epigallocatechin-3-O-gallate
-
-
Fe2+
-
88%, 68% and 80% inhibition of isoenzymes Ia, Ib, and II, respectively
FeCl3
-
markedly inhibits PPO
gallocatechin gallate
-
-
geranyl acetate
-
slight inhibition
geranyl gallate
glabrene
glabridin
glutathione
glycine methyl ester hydrochloride
-
irreversible inactivation, second-order rate constants
GSH
-
increasing the concentration from 0 to 300 mM results in a high inhibitory effect on enzyme activity, mostly due to a drop of pH of the reaction solution to acidic values. Upon heating GSH at 90°C, thermal degradation product formation is responsible for a partial inhibition. GSH-derived Maillard reaction products highly inhibit enzyme activity, inhibition efficiency increasing with heating time, 2-39 h and temperature, 80-100 °C
hexadecyltrimethyl-ammonium bromide
-
-
Hg2+
-
1 mM, 84% inhibition
iodoacetate
-
1 mM; 1 mM: 46%, 39% and 31% inhibition of isoenzymes Ia, Ib, and II, respectively
isoascorbic acid
isoliquiritigenin
kaempferol
kojic acid
L-ascorbic acid
L-Cys
-
competitive with 4-methylcatechol, catechol or pyrogallol. IC50: 0.125 mM in reaction with 4-methylcatechol, IC50: 0.637 mM in reaction with pyrogallol, IC50: 0.15 mM in reaction with catechol
L-cysteine
L-cysteine chloride
L-mimosine
luteolin
Luteolin 7-O-glucoside
m-hydroxybenzoic acid
Maillard reaction products
-
potential natural inhibitors for use with minimally processed fruits
-
Metabisulfite
Methimazole
mimosine
-
1 mM, 88%, 79% and 82% inhibition of isoenzymes Ia, Ib, and II, respectively
Mn2+
-
inhibits activity at 0.01 mM
myrcene
-
competitive inhibitor
N,N-diethyldithiocarbamate
-
94.4% inhibition at 0.0293 mM
N-(2,4-dihydroxybenzyl)-2,4-dihydroxybenzamide
-
IC50: 0.029 mM
N-(2,4-dihydroxybenzyl)-3,4,5-trihydroxybenzamide
-
IC50: 0.017 mM
N-(2,4-dihydroxybenzyl)-3,4-dihydroxybenzamide
-
IC50: 0.011 mM
N-(2,4-dihydroxybenzyl)-3,5-dihydroxybenzamide
-
IC50: 0.0022 mM
N-benzyl-2,4-dihydroxybenzamide
-
IC50: 1.660 mM
N-benzyl-3,4,5-trihydroxybenzamide
-
IC50: 0.780 mM
N-benzyl-3,4-dihydroxybenzamide
-
IC50: 2.0 mM
N-benzyl-3,5-dihydroxybenzamide
-
IC50: 0.700 mM
N-benzylamide
-
IC50: 1.990 mM
N-benzylbenzamide derivatives
-
inhibitory potency, structure-activity relationships, overview
-
N-bromosuccinimide
-
-
NaHSO3
Naphthol
-
strong inhibition of the reaction with catechol
neryl acetate
-
slight inhibition
o-hydroxybenzoic acid
o-phenanthroline
-
-
orcinol
-
strong inhibition of the reaction with catechol
oxalic acid
-
-
p-aminobenzenesulfonamide
-
-
p-hydroxybenzoic acid
p-nitrophenol
-
strong inhibition of the reaction with catechol
papain
-
proteolytic inactivation
-
phenyl-2,2'-methylenebis-(5,5-dimethylcyclohexane-1,3-dione)
-
IC50: 0.0026 mM
Phenylthiourea
polyvinylpyrrolidone 40
-
-
-
procyanidin
-
inhibition intensity increases with NAD+. The inhibitory effect of oxidized procyanidins is twice that of native procyanidins
quercetin
resorcinol
-
10 mM, 40% inhibition
Sabinene
-
slight inhibition
Salicylhydroxamic acid
-
-
salicylic acid
-
uncompetitive
Sn2+
-
1 mM, 99% inhibition
SnCl2
-
markedly inhibits PPO
Sodium azide
Sodium bisulfite
-
1 mM, 97% inhibition
Sodium cyanide
-
noncompetitive
Sodium diethyl dithiocarbamate
sodium disulfite
-
-
Sodium metabisulfite
sodium sulfite
-
complete inhibition at 10 mM
succinic acid
-
complete inhibition at 1 mM
sulfonamide compounds
-
-
-
syringic acid
-
-
tartaric acid
-
2% residual activity at 400 mM in cultivar Violetto di Sicilia, 18% residual activity at 400 mM in cultivar Violetto di Provenza, 29% residual activity at 400 mM in cultivar Tema 2000
Thiourea
tropolone
tyramine
-
typical inhibitors of catecholoxidase, also inhibit the phenoloxidase activity of activated hemocyanin
Xanthogenate
-
1 mM, 94% inhibition
ZnSO4
-
-