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Information on EC 1.1.9.1 - alcohol dehydrogenase (azurin) and Organism(s) Pseudomonas putida and UniProt Accession Q8GR64
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1.1.9.1 alcohol dehydrogenase (azurin)IUBMB Comments
A soluble, periplasmic PQQ-containing quinohemoprotein. Also contains a single heme c. Occurs in Comamonas and Pseudomonas. Does not require an amine activator. Oxidizes a wide range of primary and secondary alcohols, and also aldehydes and large substrates such as sterols; methanol is not a substrate. Usually assayed with phenazine methosulfate or ferricyanide. Like all other quinoprotein alcohol dehydrogenases it has an 8-bladed 'propeller' structure, a calcium ion bound to the PQQ in the active site and an unusual disulfide ring structure in close proximity to the PQQ.
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The taxonomic range for the selected organisms is: Pseudomonas putida
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
ADH IIB, ADH IIG, EC 1.1.98.1, QH-ADH, quinohaemoprotein alcohol dehydrogenase, quinohemoprotein alcohol dehydrogenase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
alcohol:azurin oxidoreductase
A soluble, periplasmic PQQ-containing quinohemoprotein. Also contains a single heme c. Occurs in Comamonas and Pseudomonas. Does not require an amine activator. Oxidizes a wide range of primary and secondary alcohols, and also aldehydes and large substrates such as sterols; methanol is not a substrate. Usually assayed with phenazine methosulfate or ferricyanide. Like all other quinoprotein alcohol dehydrogenases it has an 8-bladed 'propeller' structure, a calcium ion bound to the PQQ in the active site and an unusual disulfide ring structure in close proximity to the PQQ.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1,2-butanediol + ferricyanide
2-hydroxybutanal + ferrocyanide
-
ADH IIB, 84% of the activity with 1-butanol. ADH IIG, 86% of the activity with 1,2-propanediol
-
-
?
1,2-ethanediol + ferricyanide
hydroxyacetaldehyde + ferrocyanide
-
ADH IIB, 0% of the activity with 1-butanol. ADH IIG, 71% of the activity with 1,2-propanediol
-
-
?
1,2-propanediol + ferricyanide
2-hydroxypropanal + ferrocyanide
-
ADH IIB, 43% of the activity with 1-butanol. ADH IIG, maximum activity
-
-
?
1,3-butanediol + ferricyanide
3-hydroxybutanal + ferrocyanide
-
ADH IIB, 105% of the activity with 1-butanol. ADH IIG, 75% of the activity with 1,2-propanediol
-
-
?
1,3-propandione + ferricyanide
3-hydroxypropanal + ferrocyanide
-
ADH IIB, 61% of the activity with 1-butanol. ADH IIG, 42% of the activity with 1,2-propanediol
-
-
?
1,4-butanediol + ferricyanide
4-hydroxybutanal + ferrocyanide
-
ADH IIB, 110% of the activity with 1-butanol. ADH IIG, 75% of the activity with 1,2-propanediol
-
-
?
1-butanol + ferricyanide
butanal + ferrocyanide
-
ADH IIG, 42% of the activity with 1,2-propanediol
-
-
?
1-heptanol + ferricyanide
heptanal + ferrocyanide
-
ADH IIB, 99% of the activity with 1-butanol. ADH IIG, 39% of the activity with 1,2-propanediol
-
-
?
1-hexanol + ferricyanide
hexanal + ferrocyanide
-
ADH IIB, 70% of the activity with 1-butanol. ADH IIG, 46% of the activity with 1,2-propanediol
-
-
?
1-octanol + ferricyanide
octanal + ferrocyanide
-
ADH IIB, 44% of the activity with 1-butanol. ADH IIG, 24% of the activity with 1,2-propanediol
-
-
?
1-pentanol + ferricyanide
pentanal + ferrocyanide
-
ADH IIB, 100% of the activity with 1-butanol. ADH IIG, 40% of the activity with 1,2-propanediol
-
-
?
1-propanol + ferricyanide
propanal + ferrocyanide
-
ADH IIB, 70% of the activity with 1-butanol. ADH IIG, 41% of the activity with 1,2-propanediol
-
-
?
2,3-butanediol + ferricyanide
3-hydroxybutan-2-one + ferrocyanide
-
ADH IIB, 12% of the activity with 1-butanol. ADH IIG, 71% of the activity with 1,2-propanediol
-
-
?
2,4-pentanediol + ferricyanide
4-hydroxypentan-2-one + ferrocyanide
-
ADH IIB, 45% of the activity with 1-butanol. ADH IIG, 0% of the activity with 1,2-propanediol
-
-
?
2-butanol + ferricyanide
butanone + ferrocyanide
-
ADH IIB, 90% of the activity with 1-butanol. ADH IIG, 15% of the activity with 1,2-propanediol
-
-
?
2-hexanol + ferricyanide
hexan-2-one + ferrocyanide
-
ADH IIB, 99% of the activity with 1-butanol. ADH IIG, 33% of the activity with 1,2-propanediol
-
-
?
2-pentanol + ferricyanide
pentan-2-one + ferrocyanide
-
ADH IIB, 120% of the activity with 1-butanol. ADH IIG, 19% of the activity with 1,2-propanediol
-
-
?
2-propanol + ferricyanide
acetone + ferrocyanide
-
ADH IIB, 52% of the activity with 1-butanol. ADH IIG, 0% of the activity with 1,2-propanediol
-
-
?
3-pentanol + ferricyanide
pentan-3-one + ferrocyanide
-
ADH IIB, 43% of the activity with 1-butanol. ADH IIG, 15% of the activity with 1,2-propanediol
-
-
?
allyl alcohol + ferricyanide
prop-2-enal + ferrocyanide
-
ADH IIB, 160% of the activity with 1-butanol. ADH IIG, 46% of the activity with 1,2-propanediol
-
-
?
butanal + ferrocyanide
1-butanol + ferricyanide
-
ADH IIB, 32% of the activity with 1-butanol. ADH IIG, 13% of the activity with 1,2-propanediol
-
-
?
ethanal + ferrocyanide
ethanol + ferricyanide
-
ADH IIB, 78% of the activity with 1-butanol. ADH IIG, 16% of the activity with 1,2-propanediol
-
-
?
ethanol + ferricyanide
ethanal + ferrocyanide
-
ADH IIB, 78% of the activity with 1-butanol. ADH IIG, 22% of the activity with 1,2-propanediol
-
-
?
glycerol + ferrocyanide
2,3-dihydroxypropanal + ferricyanide
-
ADH IIB, 0% of the activity with 1-butanol. ADH IIG, 63% of the activity with 1,2-propanediol
-
-
?
iso-amyl alcohol + ferricyanide
3-methylbutanol + ferrocyanide
-
ADH IIB, 100% of the activity with 1-butanol. ADH IIG, 93% of the activity with 1,2-propanediol
-
-
?
propanal + ferrocyanide
1-propanol + ferricyanide
-
ADH IIB, 125% of the activity with 1-butanol. ADH IIG, 58% of the activity with 1,2-propanediol
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
-
ADH IIB is formed in the cells grown on alcohols of medium chain length and also on 1,3-butanediol. Induction of ADH IIG is restricted to 1,2-propanediol or glycerol, of which the former alcohol is more effective
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Toyama, H.; Aoki, N.; Matsushita, K.; Adachi, O.
Azurin involved in alcohol oxidation system in Pseudomonas putida HK5: expression analysis and gene cloning.
Biosci. Biotechnol. Biochem.
65
1617-1626
2001
Pseudomonas putida (Q8GR64)
EXTERNAL LINKS (specific for EC-Number 1.1.9.1)
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Release 2023.1 (January 2023)
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