Information on EC 1.1.3.7 - aryl-alcohol oxidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.3.7
-
RECOMMENDED NAME
GeneOntology No.
aryl-alcohol oxidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an aromatic primary alcohol + O2 = an aromatic aldehyde + H2O2
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
aryl-alcohol:oxygen oxidoreductase
Oxidizes many primary alcohols containing an aromatic ring; best substrates are (2-naphthyl)methanol and 3-methoxybenzyl alcohol.
CAS REGISTRY NUMBER
COMMENTARY hide
9028-77-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
strain MTCC 6324
-
-
Manually annotated by BRENDA team
strain MTCC 6324
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
; encoded by sao gene
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
no activity in Fusarium oxysporum
strain BAFC 738
-
-
Manually annotated by BRENDA team
no activity in Fusarium oxysporum BAFC 738
strain BAFC 738
-
-
Manually annotated by BRENDA team
no activity in Inocutis jamaicensis
-
-
-
Manually annotated by BRENDA team
no activity in Phanerochaete chrysosporium
no activity in Phanerochaete chrysosporium NRRL 6361
strain NRRL 6361
-
-
Manually annotated by BRENDA team
Pleurotus laciniatocrenatus
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
metabolism
-
aryl-alcohol oxidase, AAO, participates in fungal degradation of lignin, a process of high ecological and biotechnological relevance, by providing the hydrogen peroxide required by ligninolytic peroxidases, mechanism, overview
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R,S)-4-methoxybenzyl alcohol + O2
1-(4-methoxyphenyl)ethanol + H2O2
show the reaction diagram
-
over 98% excess of the R enantiomer after treatment of racemic 1-(4-methoxyphenyl)ethanol, the hydride transfer is highly stereoselective
-
-
?
(S)-1-(4-fluorophenyl)ethanol + O2
1-(4-fluorophenyl)acetaldehyde + H2O2
show the reaction diagram
-
mutant F501A
-
-
?
1,1'-binaphthalene + O2
?
show the reaction diagram
1,2,3,4,5-pentachlorobenzene + O2
?
show the reaction diagram
1,2,3,4-tetrachlorobenzene + O2
?
show the reaction diagram
1,2,4,5-tetrachlorobenzene + O2
?
show the reaction diagram
1,2-binaphthalene + O2
?
show the reaction diagram
1-(2-naphthalenylmethyl)-naphthalene + O2
?
show the reaction diagram
1-amino-9,10-anthracenedione + O2
?
show the reaction diagram
1-chloro-9,10-anthracenedione + O2
?
show the reaction diagram
1-naphthalene methanol + O2
alpha-naphthaldehyde + H2O2
show the reaction diagram
-
27% of the activity with cinnamyl alcohol
-
-
?
2,4-dichloroaniline + O2
?
show the reaction diagram
2,4-dimethoxybenzyl alcohol + O2
2,4-dimethoxybenzaldehyde + H2O2
show the reaction diagram
2,4-dimethoxybenzyl alcohol + O2
2,4-dimethoxybenzyl aldehyde + H2O2
show the reaction diagram
-
50% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 75% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II
-
-
?
2,4-hexadien-1-ol + O2
2,4-hexadienal + H2O2
show the reaction diagram
2,4-hexadien-1-ol + O2
?
show the reaction diagram
-
-
-
?
2,4-hexadien-1-ol + O2
? + H2O2
show the reaction diagram
-
531% of the activity with benzyl alcohol
-
?
2,4-hexadienal + O2
2,4-hexadienoate + H2O2
show the reaction diagram
-
-
-
-
?
2,6-dichloroaniline + O2
?
show the reaction diagram
2-hydroxybenzyl alcohol + O2
2-hydroxybenzaldehyde + H2O2
show the reaction diagram
2-methoxybenzyl alcohol + O2
2-methoxybenzaldehyde + H2O
show the reaction diagram
-
23% of the activity with 2-hydroxybenzyl alcohol
-
?
2-methoxybenzyl alcohol + O2
2-methoxybenzylaldehyde + H2O
show the reaction diagram
2-methylbenzyl alcohol + O2
2-methylbenzylaldehyde + H2O
show the reaction diagram
2-naphthalenemethanol + O2
2-naphthaleneformaldehyde + H2O
show the reaction diagram
-
745.7% of the activity with benzyl alcohol
-
?
2-naphthalenemethanol + O2
?
show the reaction diagram
-
-
-
-
?
2-naphthylmethanol + O2
2-naphthaldehyde + H2O2
show the reaction diagram
2-phenylethyl alcohol + O2
2-phenylacetaldehyde + H2O
show the reaction diagram
3,4-difluorobenzaldehyde + O2
3,4-difluorobenzoic acid
show the reaction diagram
-
-
-
-
?
3,4-difluorobenzaldehyde + O2
3,4-difluorobenzoic acid + H2O2
show the reaction diagram
-
-
-
-
r
3,4-dimethoxybenzyl alcohol + O2
3,4-dimethoxybenzaldehyde + H2O2
show the reaction diagram
3,4-dimethoxybenzyl alcohol + O2
veratryl aldehyde + H2O2
show the reaction diagram
3,5-dimethoxybenzyl alcohol + O2
3,5-dimethoxy benzaldehyde + H2O2
show the reaction diagram
-
7% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 8% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II
-
-
?
3-anisyl alcohol + O2
3-anisyl aldehyde + H2O2
show the reaction diagram
3-chloro-4-anisaldehyde + O2
3-chloro-4-anisic acid + H2O2
show the reaction diagram
-
-
-
-
r
3-chloro-4-anisyl alcohol + O2
3-chloro-4-anisaldehyde + H2O2
show the reaction diagram
-
-
-
-
?
3-chloro-4-anisyl alcohol + O2
3-chloro-4-anisyl aldehyde + H2O2
show the reaction diagram
-
high activity
-
-
?
3-chlorobenzaldehyde + O2
3-chlorobenzoic acid + H2O2
show the reaction diagram
-
-
-
-
r
3-chlorobenzyl alcohol + O2
3-chlorobenzaldehyde + H2O2
show the reaction diagram
-
-
-
-
?
3-chlorobenzyl alcohol + O2
3-chlorobenzyl aldehyde + H2O2
show the reaction diagram
-
-
-
-
?
3-fluorobenzaldehyde + O2
3-fluorobenzoic acid + H2O2
show the reaction diagram
-
-
-
-
r
3-fluorobenzyl alcohol + O2
3-fluorobenzaldehyde + H2O2
show the reaction diagram
-
-
-
-
?
3-fluorobenzyl alcohol + O2
3-fluorobenzyl aldehyde + H2O2
show the reaction diagram
-
low activity
-
-
?
3-fluorobenzyl alcohol + O2
?
show the reaction diagram
-
-
-
?
3-hydroxy-4-methoxybenzyl alcohol + O2
3-hydroxy-4-methoxybenzaldehyde + H2O2
show the reaction diagram
-
62% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 71% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II
-
-
?
3-hydroxybenzyl alcohol + O2
3-hydroxybenzaldehyde + H2O2
show the reaction diagram
3-methoxybenzyl alcohol + O2
3-methoxybenzaldehyde + H2O2
show the reaction diagram
3-methoxybenzyl alcohol + O2
3-methoxybenzylaldehyde + H2O
show the reaction diagram
recombinant enzyme shows 1% of the activity with 2-hydroxybenzyl alcohol
-
-
?
3-phenoxybenzyl alcohol + O2
3-phenoxybenzaldehyde + H2O2
show the reaction diagram
-
35% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 18% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II
-
-
?
4-anisaldehyde + O2
4-anisic acid + H2O2
show the reaction diagram
-
-
-
-
r
4-anisyl alcohol + O2
4-anisaldehyde + H2O2
show the reaction diagram
4-anisyl alcohol + O2
4-anisyl aldehyde + H2O2
show the reaction diagram
4-chlorobenzaldehyde + O2
4-chlorobenzoic acid + H2O2
show the reaction diagram
-
-
-
-
r
4-chlorobenzyl alcohol + O2
4-chlorobenzaldehyde + H2O2
show the reaction diagram
-
-
-
-
?
4-chlorobenzyl alcohol + O2
4-chlorobenzyl aldehyde + H2O2
show the reaction diagram
-
-
-
-
?
4-fluorobenzaldehyde + O2
4-fluorobenzoic acid + H2O2
show the reaction diagram
-
-
-
-
r
4-fluorobenzyl alcohol + O2
4-fluorobenzaldehyde + H2O2
show the reaction diagram
-
-
-
-
?
4-fluorobenzyl alcohol + O2
4-fluorobenzyl aldehyde + H2O2
show the reaction diagram
-
-
-
-
?
4-hydroxy-3-methoxybenzyl alcohol + O2
4-hydroxy-3-methoxybenzaldehyde + H2O2
show the reaction diagram
4-hydroxybenzyl alcohol + O2
4-hydroxybenzaldehyde + H2O2
show the reaction diagram
4-hydroxybenzyl alcohol + O2
4-hydroxybenzyl aldehyde + H2O2
show the reaction diagram
-
7.6% of the activity with anisyl alcohol
-
-
?
4-methoxybenzyl alcohol + O2
4-methoxybenzaldehyde + H2O2
show the reaction diagram
4-methoxycinnamyl alcohol + O2
4-methoxycinnamaldehyde + H2O2
show the reaction diagram
-
-
-
-
r
4-nitrobenzaldehyde + O2
4-nitrobenzoic acid
show the reaction diagram
-
-
-
-
?
4-nitrobenzaldehyde + O2
4-nitrobenzoic acid + H2O2
show the reaction diagram
-
-
-
-
r
4-nitrobenzyl alcohol + O2
4-nitrobenzaldehyde + H2O2
show the reaction diagram
-
-
-
-
r
7H-benz[DE]anthracen-7-one + O2
?
show the reaction diagram
9,10-anthracenedione + O2
?
show the reaction diagram
anisyl alcohol + O2
anisaldehyde + H2O2
show the reaction diagram
anisyl alcohol + O2
anisyl aldehyde + H2O2
show the reaction diagram
-
-
-
-
?
benzaldehyde + O2
benzoic acid + H2O2
show the reaction diagram
-
-
-
-
r
benzyl alcohol + O2
benzaldehyde + H2O2
show the reaction diagram
beta-naphthylcarbinol + O2
beta-naphthaldehyde + H2O2
show the reaction diagram
cinnamyl alcohol + O2
cinnamaldehyde + H2O2
show the reaction diagram
cinnamyl alcohol + O2
cinnamic aldehyde + H2O2
show the reaction diagram
cinnamyl alcohol + O2
cinnamyl aldehyde + H2O2
show the reaction diagram
-
-
-
-
?
coniferyl alcohol + O2
coniferyl aldehyde + H2O2
show the reaction diagram
-
13% of the activity with cinnamyl alcohol
-
-
?
cyclohexyl alcohol + O2
cyclohexyl aldehyde + H2O2
show the reaction diagram
-
12% of the activity with cinnamyl alcohol
-
-
?
diphenylether + O2
?
show the reaction diagram
diphenylsulfone + O2
?
show the reaction diagram
Direct Red 5B + O2
3-diazenyl-7-[(phenylcarbonyl)amino]naphthalene-2-sulfonic acid + H2O2
show the reaction diagram
-
degradation, dye decolorizing
product identification by GC-MS analysis
-
?
ethanol + O2
acetaldehyde + H2O2
show the reaction diagram
isovanillyl alcohol + O2
isovanillyl aldehyde + H2O2
show the reaction diagram
-
-
-
-
?
m-anisyl alcohol + O2
m-anisaldehyde + H2O2
show the reaction diagram
N-phenyl-1-naphthalenamine + O2
?
show the reaction diagram
n-propanol + O2
propionaldehyde + H2O2
show the reaction diagram
naphthalene + O2
?
show the reaction diagram
veratraldehyde + O2
veratric acid + H2O2
show the reaction diagram
-
-
-
-
r
veratryl alcohol + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
veratryl alcohol + O2
veratraldehyde + H2O2
show the reaction diagram
veratryl alcohol + O2
veratryl aldehyde + H2O2
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(R,S)-4-methoxybenzyl alcohol + O2
1-(4-methoxyphenyl)ethanol + H2O2
show the reaction diagram
-
over 98% excess of the R enantiomer after treatment of racemic 1-(4-methoxyphenyl)ethanol, the hydride transfer is highly stereoselective
-
-
?
1,1'-binaphthalene + O2
?
show the reaction diagram
-
degradation, partial removal from soil
-
-
?
1,2,3,4,5-pentachlorobenzene + O2
?
show the reaction diagram
-
degradation, partial removal from soil
-
-
?
1,2,3,4-tetrachlorobenzene + O2
?
show the reaction diagram
-
degradation, complete removal from soil
-
-
?
1,2,4,5-tetrachlorobenzene + O2
?
show the reaction diagram
-
degradation, complete removal from soil
-
-
?
1,2-binaphthalene + O2
?
show the reaction diagram
-
degradation, partial removal from soil
-
-
?
1-(2-naphthalenylmethyl)-naphthalene + O2
?
show the reaction diagram
-
degradation, partial removal from soil
-
-
?
1-amino-9,10-anthracenedione + O2
?
show the reaction diagram
-
degradation, partial removal from soil
-
-
?
1-chloro-9,10-anthracenedione + O2
?
show the reaction diagram
-
degradation, partial removal from soil
-
-
?
2,4-dichloroaniline + O2
?
show the reaction diagram
-
degradation, complete removal from soil
-
-
?
2,4-dimethoxybenzyl alcohol + O2
2,4-dimethoxybenzaldehyde + H2O2
show the reaction diagram
-
-
-
-
r
2,4-hexadien-1-ol + O2
?
show the reaction diagram
O94219
-
-
-
?
2,6-dichloroaniline + O2
?
show the reaction diagram
-
degradation, complete removal from soil
-
-
?
2-hydroxybenzyl alcohol + O2
2-hydroxybenzaldehyde + H2O2
show the reaction diagram
2-naphthylmethanol + O2
2-naphthaldehyde + H2O2
show the reaction diagram
-
best substrate
-
-
r
3-methoxybenzyl alcohol + O2
3-methoxybenzaldehyde + H2O2
show the reaction diagram
-
-
-
-
r
4-anisyl alcohol + O2
4-anisaldehyde + H2O2
show the reaction diagram
O94219
the substrate is an extracellular fungal metabolite
-
-
?
4-anisyl alcohol + O2
4-anisyl aldehyde + H2O2
show the reaction diagram
-
preferred substrate
-
-
?
4-hydroxy-3-methoxybenzyl alcohol + O2
4-hydroxy-3-methoxybenzaldehyde + H2O2
show the reaction diagram
-
-
-
-
r
4-hydroxybenzyl alcohol + O2
4-hydroxybenzaldehyde + H2O2
show the reaction diagram
-
-
-
-
r
4-methoxybenzyl alcohol + O2
4-methoxybenzaldehyde + H2O2
show the reaction diagram
4-methoxycinnamyl alcohol + O2
4-methoxycinnamaldehyde + H2O2
show the reaction diagram
-
-
-
-
r
4-nitrobenzyl alcohol + O2
4-nitrobenzaldehyde + H2O2
show the reaction diagram
-
-
-
-
r
7H-benz[DE]anthracen-7-one + O2
?
show the reaction diagram
-
degradation, partial removal from soil
-
-
?
9,10-anthracenedione + O2
?
show the reaction diagram
-
degradation, partial removal from soil
-
-
?
benzyl alcohol + O2
benzaldehyde + H2O2
show the reaction diagram
cinnamyl alcohol + O2
cinnamaldehyde + H2O2
show the reaction diagram
-
high activity
-
-
r
diphenylether + O2
?
show the reaction diagram
-
degradation, complete removal from soil
-
-
?
diphenylsulfone + O2
?
show the reaction diagram
-
degradation, partial removal from soil
-
-
?
N-phenyl-1-naphthalenamine + O2
?
show the reaction diagram
-
degradation, complete removal from soil
-
-
?
n-propanol + O2
propionaldehyde + H2O2
show the reaction diagram
naphthalene + O2
?
show the reaction diagram
-
degradation, complete removal from soil
-
-
?
veratryl alcohol + O2
veratraldehyde + H2O2
show the reaction diagram
veratryl alcohol + O2
veratryl aldehyde + H2O2
show the reaction diagram
Pleurotus laciniatocrenatus
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
flavin
-
prosthetic group
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cu2+
-
required for catalysis
additional information
Pleurotus laciniatocrenatus
-
no activation or induction by supplementation of CuSO4
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-hydroxybenzyl alcohol
3-Phenyl-1-propanol
4-anisic acid
-
competitive
4-methoxybenzylamine
-
uncompetitive, pH-dependent inhibition, best at pH 8.0
Ag+
-
0.5 mM, 38% inhibition
benzylmethyl ether
-
competitive
CaCl2
-
15% inhibition at 0.5 mM
chavicol
-
competitive
dithiothreitol
-
7% inhibition at 0.5 mM
EDTA
-
24% inhibition at 0.5 mM
HgCl2
-
1 mM, 92% inhibition
L-cysteine
-
20% inhibition at 0.5 mM
MgCl2
-
16% inhibition at 0.5 mM
MnCl2
-
14% inhibition at 0.5 mM
NaN3
-
10 mM, 19% inhibition
p-methoxybenzyl alcohol
-
substrate inhibition at high concentrations
Pb2+
-
0.5 mM, 63% inhibition
PCMB
-
1 mM, 95% inhibition
phenol
-
competitive
Sodium azide
-
mixed competitive inhibition, 90% inhibition at 0.5 mM
Toluene
-
competitive
ZnSO4
-
8% inhibition at 0.5 mM
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
Pleurotus laciniatocrenatus
-
enzyme is induced by glucose depletion, no activation or induction by supplementation of vanillic acid
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.79
2,4-Dimethoxybenzyl alcohol
-
pH 6.0, 25°C
0.081 - 0.263
2,4-hexadien-1-ol
13
2,4-hexadienal
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
28 - 132
2-hydroxybenzyl alcohol
3
3,4-difluorobenzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
0.39 - 1.5
3,4-dimethoxybenzyl alcohol
0.211 - 0.734
3-anisyl alcohol
0.7
3-chloro-4-anisaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
0.014
3-chloro-4-anisyl alcohol
-
pH 6.0, 24°C
1.5
3-Chlorobenzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
0.107
3-Chlorobenzyl alcohol
-
pH 6.0, 24°C
2.2
3-Fluorobenzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
0.554
3-fluorobenzyl alcohol
-
pH 6.0, 24°C
0.22
3-Methoxybenzyl alcohol
-
pH 6.0, 25°C
0.7 - 0.8
4-anisaldehyde
0.015 - 0.053
4-anisyl alcohol
4.7
4-Chlorobenzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
0.132
4-Chlorobenzyl alcohol
-
pH 6.0, 24°C
4.9
4-Fluorobenzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
0.553
4-fluorobenzyl alcohol
-
pH 6.0, 24°C
0.017 - 3.82
4-methoxybenzyl alcohol
2 - 5
4-Nitrobenzaldehyde
0.108 - 0.836
anisyl alcohol
7
benzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
0.03 - 5
benzyl alcohol
0.708
cinnamyl alcohol
-
pH 6.0, 24°C
0.831
isovanillyl alcohol
-
pH 6.0, 24°C
0.22 - 0.3
m-anisyl alcohol
0.017 - 0.348
O2
0.03 - 0.04
p-anisyl alcohol
0.025
Vanillyl alcohol
-
-
8
veratraldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
0.027 - 2
veratryl alcohol
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1 - 206
2,4-hexadien-1-ol
0.33
2,4-hexadienal
Pleurotus eryngii
-
wild type enzyme, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
0.867
3,4-difluorobenzaldehyde
Pleurotus eryngii
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
30 - 82.2
3,4-dimethoxybenzyl alcohol
1 - 26
3-anisyl alcohol
0.057
3-chloro-4-anisaldehyde
Pleurotus eryngii
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
46
3-chloro-4-anisyl alcohol
Pleurotus eryngii
-
pH 6.0, 24°C
0.85
3-Chlorobenzaldehyde
Pleurotus eryngii
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
22
3-Chlorobenzyl alcohol
Pleurotus eryngii
-
pH 6.0, 24°C
0.883
3-Fluorobenzaldehyde
Pleurotus eryngii
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
6
3-fluorobenzyl alcohol
Pleurotus eryngii
-
pH 6.0, 24°C
0.012 - 0.05
4-anisaldehyde
3 - 142
4-anisyl alcohol
1.05
4-Chlorobenzaldehyde
Pleurotus eryngii
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
51
4-Chlorobenzyl alcohol
Pleurotus eryngii
-
pH 6.0, 24°C
0.367
4-Fluorobenzaldehyde
Pleurotus eryngii
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
32
4-fluorobenzyl alcohol
Pleurotus eryngii
-
pH 6.0, 24°C
0.069 - 208
4-methoxybenzyl alcohol
1.21 - 1.633
4-Nitrobenzaldehyde
0.5
benzaldehyde
Pleurotus eryngii
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
1 - 33
benzyl alcohol
65
cinnamyl alcohol
Pleurotus eryngii
-
pH 6.0, 24°C
127
isovanillyl alcohol
Pleurotus eryngii
-
pH 6.0, 24°C
8.27
m-anisyl alcohol
Pleurotus pulmonarius
-
-
48.5
p-anisyl alcohol
Pleurotus pulmonarius
-
-
0.13
veratraldehyde
Pleurotus eryngii
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
3 - 116
veratryl alcohol
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.025
2,4-hexadienal
Pleurotus eryngii
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
11092
0.282
3,4-difluorobenzaldehyde
Pleurotus eryngii
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
17581
0.085
3-chloro-4-anisaldehyde
Pleurotus eryngii
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
28105
0.643
3-Chlorobenzaldehyde
Pleurotus eryngii
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
2459
0.407
3-Fluorobenzaldehyde
Pleurotus eryngii
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
3968
0.013 - 0.087
4-anisaldehyde
14732
0.223
4-Chlorobenzaldehyde
Pleurotus eryngii
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
2129
0.075
4-Fluorobenzaldehyde
Pleurotus eryngii
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
3057
0.019 - 5120
4-methoxybenzyl alcohol
1158
0.315 - 0.597
4-Nitrobenzaldehyde
759
0.073
benzaldehyde
Pleurotus eryngii
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
146
0.0167
veratraldehyde
Pleurotus eryngii
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
3852
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.48
3-Phenyl-1-propanol
0.08
4-anisic acid
0.25
4-methoxybenzylamine
-
pH 8.0, 24°C
1.35
benzylmethyl ether
-
pH 6.0, 24°C
0.11
chavicol
-
pH 6.0, 24°C
1.92
phenol
0.75
Toluene
-
pH 6.0, 24°C
additional information
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.155
-
crude enzyme, using veratryl alcohol as substrate, at pH 6.0, 40°C
0.66
-
soil-extracted extracellular enzyme
1.58
-
after 10.19fold purification, using veratryl alcohol as substrate, at pH 6.0, 40°C
21.75
-
purified native enzyme, pH 3.0, 40°C
41.5
-
pH 6.0, 30°C, isozyme III
60.2
-
pH 6.0, 30°C, isozyme I
63.6
-
pH 6.0, 30°C, isozyme II
96
purified recombinant enzyme from Escherichia coli
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 8
-
pH 3.5: about 70% of maximal activity, pH 8.0: about 65% of maximal activity
4 - 9
-
AAO shows no pH dependence of kcat or catalytic efficiency for the substrates analyzed, AAO is unstable above pH 9.0
4 - 7
-
the enzyme displays over 63% of its activity in the pH range of 4.0-7.0. A further increase of these pH values decreases the relative activity to 17%
5.3 - 7.3
-
pH 5.3: about 90% of maximal activity, pH 7.3: 35% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 60
-
20°C: about 55% of maximal activity, 60°C: about 50% of maximal activity
30 - 60
-
the enzyme completely loses its activity at 60°C
47
-
90% of maximal activity within this range
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5
-
isoelectric focussing, slight differences for isozymes I, II, III
3.9
-
isoelectric focusing, pH-gradient: 2.5-5.0
5.2
-
isoelectric focusing, pH-gradient: 3-6
5.4
-
isoelectric focusing, pH-gradient: 3-6
6.1
calculated from sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
61847
x * 61847, recombinant enzyme from Escherichia coli, mass spectrometry, x * 69114, wild-type enzyme, mass spectrometry, x * 69792, recombinant enzyme from Emericella nidulans, mass spectrometry
67000
-
gel filtration
67100
x * 67100, CtSAO without signal peptide, calculated from sequence
69114
x * 61847, recombinant enzyme from Escherichia coli, mass spectrometry, x * 69114, wild-type enzyme, mass spectrometry, x * 69792, recombinant enzyme from Emericella nidulans, mass spectrometry
69792
x * 61847, recombinant enzyme from Escherichia coli, mass spectrometry, x * 69114, wild-type enzyme, mass spectrometry, x * 69792, recombinant enzyme from Emericella nidulans, mass spectrometry
70500
-
1 * 70500, SDS-PAGE
71200
-
x * 71200, SDS-PAGE
72000
-
1 * 72000, SDS-PAGE, isozymes i, II, III
72500
-
1 * 72500, SDS-PAGE
72600
-
1 * 72600, SDS-PAGE
78000
-
gel filtration
79000
-
4 * 79000, SDS-PAGE
272000
-
native PAGE
334000
-
gel filtration
additional information
apparent mass of about 80 kDa determined by SDS-PAGE, molecular mass of 67.7 kDa deduced from sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
tetramer
-
4 * 79000, SDS-PAGE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method, using 1 M Li2SO4, 0.1 M bis-Tris propane pH 7.4
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 9
-
24 h, stable
389839
5 - 8
-
30°C, 15 min, stable
389849
9
-
stable up to, unstable above
698931
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
highly decreased stability beyond 30°C
45
-
5 min, stable
55
-
5 min, complete inactivation
65
-
5 min, total inactivation
70
-
10 min, complete inactivation
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C or -18°C, pH 5.7, stable for several weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 enzyme forms: VAO I and VAO II
-
; gel filtration
native enzyme 24.4fold to homogeneity by anion exchange chromatography and gel filtration
-
partially by microsome preparation
-
recombinant enzyme
-
recombinant enzyme from Emericella nidulans by gel filtration and anion exchange chromatography
-
recombinant FLAG1-tagged enzyme in Escherichia coli strain W3110 solubilized from inclusion bodies 9.6fold by anion exchange chromatography
recombinant mature enzyme from Escherichia coli by anion exchange chromatography
-
recombinant wild-type and mutant emzymes from Escherichia coli to homogeneity
-
three-phase partitioning with 30% (w/v) of ammonium sulfate and t-butanol (1:1 ratio)
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic tree
DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic tree, recombinant expression in Escherichia coli
-
expressed in Escherichia coli
-
expression in Aspergillus nidulans, activity of the recombinant enzyme in Aspergillus nidulans culture is much higher than in extracellular fluid of Pleurotus eryngii
-
expression in Emericella nidulans under control of the fungal alc promoter
-
expression in Escherichia coli; expression of the constructs encoding CpSAO in Escherichia coli BL21(DE3) results in the formation of insoluble inclusion bodies. The expression construct is coexpressed with the plasmid pREP4-groESL, encoding chaperonine proteins of the Hsp60 family (GroEL and GroES). Coexpression at 15°C for 16 h results in the formation of soluble recombinant SAO
expression in Escherichia coli; expression of the constructs encoding CtSAO in Escherichia coli BL21(DE3) results in the formation of insoluble inclusion bodies. The expression construct is coexpressed with the plasmid pREP4-groESL, encoding chaperonine proteins of the Hsp60 family (GroEL and GroES). Coexpression at 15°C for 16 h results in the formation of soluble recombinant SAO
expression of the mature enzyme in Escherichia coli
-
expression of wild-type and mutant enzymes in Emericella nidulans under control of the inducible fungal alc promoter
-
expression of wild-type enzyme in Escherichia coli, expression of wild-type and mutant enzymes in Emericella nidulans
-
gene aao, expression of FLAG1-tagged enzyme in Escherichia coli strain W3110, the non-glycosylated recombinant enzyme is successfully activated in vitro after Escherichia coli expression in form of inclusion bodies, expression iand glycosylation of the enzyme in Emericella nidulans strain IJFM A729
gene aao, expression of wild-type and mutant emzymes in Escherichia coli
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
compared with the control, inducers increased the AAO production in Pleurotus ostreatus in the following order: L-tyrosine (5 mM) > veratryl alcohol (7 mM) > benzyl alcohol (10 mM) > L-phenylalanine (5 mM) > 4-anisyl alcohol (9 mM)
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F501W
-
site-directed mutagenesis, the mutant shows a twofold increase in O2 reactivity compared to the wild-type enzyme
H502L
-
site-directed mutagenesis, inactive mutant
H502R
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
H546A
-
site-directed mutagenesis, the mutant shows over 35fold decreased both catalytic and transient-state reduction constants for 4-methoxybenzyl alcohol, as well as a strong decrease in the alcohol affinity compared to the wild-type enzyme
H546L
-
site-directed mutagenesis, inactive mutant
H546R
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
L315A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Y78A
-
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme
Y92A
-
site-directed mutagenesis, inactive mutant
additional information
-
wild-type and mutant enzymes are adsorbed on graphite electrodes or with the enzymes in solution using glassy carbon electrode as working electrode, activity analysis, overview