Information on EC 1.1.3.23 - thiamine oxidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.3.23
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RECOMMENDED NAME
GeneOntology No.
thiamine oxidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
thiamine + 2 O2 + H2O = thiamine acetic acid + 2 H2O2
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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-
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
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Thiamine metabolism
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SYSTEMATIC NAME
IUBMB Comments
thiamine:oxygen 5-oxidoreductase
A flavoprotein (FAD). The product differs from thiamine in replacement of -CH2.CH2.OH by -CH2.COOH; the two-step oxidation proceeds without the release of the intermediate aldehyde from the enzyme.
CAS REGISTRY NUMBER
COMMENTARY hide
96779-44-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
unidentified soil bacterium
ATCC 25589
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
oxythiamine + O2
? + H2O2
show the reaction diagram
pyrithiamine + O2
? + H2O2
show the reaction diagram
thiamine + O2
?
show the reaction diagram
unidentified soil bacterium
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-
-
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thiamine + O2
thiamine acetate + H2O2
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
thiamine + O2
?
show the reaction diagram
unidentified soil bacterium
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-
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
unidentified soil bacterium
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Hg2+
unidentified soil bacterium
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p-Chloromeruribenzoate
unidentified soil bacterium
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pyrithiamine
unidentified soil bacterium
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Quinacrine
unidentified soil bacterium
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Semicarbazide
unidentified soil bacterium
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.72
thiamine
unidentified soil bacterium
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5
H+
unidentified soil bacterium
-
release of H+
1.67
thiamine
unidentified soil bacterium
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thiamine acetate formed
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.18
unidentified soil bacterium
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additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 9
unidentified soil bacterium
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41500
unidentified soil bacterium
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gel filtration
52000
unidentified soil bacterium
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
unidentified soil bacterium
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1 * 49000-50000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
unidentified soil bacterium
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stable for 30 min
65
unidentified soil bacterium
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not stable above
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, 3 months
unidentified soil bacterium
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4°C, 1 week
unidentified soil bacterium
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
-
unidentified soil bacterium
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