Information on EC 1.1.3.23 - thiamine oxidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: unidentified

EC NUMBER
COMMENTARY
1.1.3.23
-
RECOMMENDED NAME
GeneOntology No.
thiamine oxidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
thiamine + 2 O2 + H2O = thiamine acetic acid + 2 H2O2
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Thiamine metabolism
-
SYSTEMATIC NAME
IUBMB Comments
thiamine:oxygen 5-oxidoreductase
A flavoprotein (FAD). The product differs from thiamine in replacement of -CH2.CH2.OH by -CH2.COOH; the two-step oxidation proceeds without the release of the intermediate aldehyde from the enzyme.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
thiamin dehydrogenase
-
-
-
-
thiamin oxidase
-
-
-
-
thiamin:oxygen 5-oxidoreductase
-
-
-
-
thiamine dehydrogenase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
96779-44-1
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
unidentified soil bacterium
ATCC 25589
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
oxythiamine + O2
? + H2O2
show the reaction diagram
unidentified soil bacterium
-
-
-
?
oxythiamine + O2
? + H2O2
show the reaction diagram
unidentified soil bacterium
-
-
-
?
oxythiamine + O2
? + H2O2
show the reaction diagram
unidentified soil bacterium
-
-
-
?
pyrithiamine + O2
? + H2O2
show the reaction diagram
unidentified soil bacterium
-
-
-
?
pyrithiamine + O2
? + H2O2
show the reaction diagram
unidentified soil bacterium
-
-
-
?
pyrithiamine + O2
? + H2O2
show the reaction diagram
unidentified soil bacterium
-
-
-
?
thiamine + O2
thiamine acetate + H2O2
show the reaction diagram
unidentified soil bacterium
-
-
-
?
thiamine + O2
thiamine acetate + H2O2
show the reaction diagram
unidentified soil bacterium
-
-
-
?
thiamine + O2
thiamine acetate + H2O2
show the reaction diagram
unidentified soil bacterium
-
reaction via an aldehyde intermediate which is not released
-
ir
thiamine + O2
?
show the reaction diagram
unidentified soil bacterium
-
-
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
thiamine + O2
?
show the reaction diagram
unidentified soil bacterium
-
-
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
FAD
unidentified soil bacterium
-
-
FAD
unidentified soil bacterium
-
8-alpha-N(1)-histidyl-FAD
FAD
unidentified soil bacterium
-
1 mol/mol enzyme; 8-alpha-N(1)-histidyl-FAD
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Cu2+
unidentified soil bacterium
-
-
Hg2+
unidentified soil bacterium
-
-
p-Chloromeruribenzoate
unidentified soil bacterium
-
-
Pyrithiamine
unidentified soil bacterium
-
-
Quinacrine
unidentified soil bacterium
-
-
Semicarbazide
unidentified soil bacterium
-
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.72
-
thiamine
unidentified soil bacterium
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
5
-
H+
unidentified soil bacterium
-
release of H+
1.67
-
thiamine
unidentified soil bacterium
-
thiamine acetate formed
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2.18
-
unidentified soil bacterium
-
-
additional information
-
unidentified soil bacterium
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
8
9
unidentified soil bacterium
-
-
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
41500
-
unidentified soil bacterium
-
gel filtration
52000
-
unidentified soil bacterium
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
monomer
unidentified soil bacterium
-
1 * 49000-50000, SDS-PAGE
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50
-
unidentified soil bacterium
-
stable for 30 min
65
-
unidentified soil bacterium
-
not stable above
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-70°C, 3 months
unidentified soil bacterium
-
4°C, 1 week
unidentified soil bacterium
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-
unidentified soil bacterium
-