Information on EC 1.1.3.16 - ecdysone oxidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.3.16
-
RECOMMENDED NAME
GeneOntology No.
ecdysone oxidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ecdysone + O2 = 3-dehydroecdysone + H2O2
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
-
-
-
-
reduction
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ecdysteroid metabolism (arthropods)
-
-
Insect hormone biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
ecdysone:oxygen 3-oxidoreductase
2,6-Dichloroindophenol can act as an acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
56803-12-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
lepidoptera
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
tobacco hornworm
-
-
Manually annotated by BRENDA team
dictyoptera
-
-
Manually annotated by BRENDA team
lepidoptera
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
ecdysone is inactivated to 3-dehydroecdysone under ecdysone oxidase, followed by NAD(P)H-dependent irreversible reduction to 3-epiecdysteroid under 3-dehydroecdysone 3alpha-reductase. On the other hand, 3-dehydroecdysone undergoes reversible reduction to ecdysone by 3-dehydroecdysone 3beta-reductase in the hemolymph
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-deoxy-20-hydroxyecdysone + O2
3-dehydro-2-deoxy-20-hydroxyecdysone + H2O2
show the reaction diagram
2-deoxyecdysone + O2
3-dehydro-2-deoxyecdysone + H2O2
show the reaction diagram
20-hydroxyecdysone + O2
3-dehydro-20-hydroxyecdysone + H2O2
show the reaction diagram
5,20-dihydroxyecdysone + O2
3-dehydro-5,20-dihydroxyecdysone + H2O2
show the reaction diagram
-
-
-
-
?
cyasterone + O2
3-dehydrocyasterone + H2O2
show the reaction diagram
ecdysone + O2
3-dehydroecdysone + H2O2
show the reaction diagram
ecdysteroids + O2
3-dehydroecdysteroids + H2O2
show the reaction diagram
inokosterone + O2
3-dehydroinokosterone + H2O2
show the reaction diagram
makisterone + O2
3-dehydromakisterone + H2O2
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
20-hydroxyecdysone + O2
3-dehydro-20-hydroxyecdysone + H2O2
show the reaction diagram
ecdysone + O2
3-dehydroecdysone + H2O2
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
HgCl2
-
above 0.001 mM
methanol
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.007
2,6-dichloroindophenol
-
-
0.031
20-hydroxyecdysone
-
-
0.0058 - 0.098
ecdysone
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0019
-
hemolymph
0.0028
-
fat body
0.0335
-
midgut
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.6
-
activity assay
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.3 - 8.6
-
pH 5.3: about 85% of activity maximum, pH 8.6: about 15% of activity maximum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
activity assay
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
64000
-
SDS-PAGE
72000
x * 72000, SDS-PAGE
190000
-
gel filtration
240000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 72000, SDS-PAGE
trimer
-
3 * 64000, gel filtration
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
-
denaturation begins above 35C
100
-
no activity detected after boiling
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
partial loss of activity by lyophilization
-
stable to precipitation with ammonium sulfate and redissolvation or dialysis
-
stable to repeated freezing and thawing
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-25C, more than 3 months
-
-85C, stable for several months
-
0C, several days
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
-
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3)
into the pSI expression vector for transfection of COS-7 cells, and into the pGEM-T vector, used as templates to construct standard curves
-