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Information on EC 1.1.3.13 - alcohol oxidase and Organism(s) Komagataella phaffii and UniProt Accession F2R038

for references in articles please use BRENDA:EC1.1.3.13
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EC Tree
     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.13 alcohol oxidase
IUBMB Comments
The enzymes from the fungi Candida methanosorbosa and several Basidiomycetes species contain an FAD cofactor [1,3]. The enzyme from the phytopathogenic fungi Colletotrichum graminicola and Colletotrichum gloeosporioides utilize a mononuclear copper-radical mechanism . The enzyme acts on primary alcohols and unsaturated alcohols, and has much lower activity with branched-chain and secondary alcohols.
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Komagataella phaffii
UNIPROT: F2R038
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Word Map
The taxonomic range for the selected organisms is: Komagataella phaffii
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
alcohol oxidase, alcohol oxidase 1, methanol oxidase, alcohol oxidase i, ethanol oxidase, peroxisomal alcohol oxidase, mod1p, mod2p, alcohol oxidase a, alcox, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ethanol oxidase
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oxidase, alcohol
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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oxidation
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reduction
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SYSTEMATIC NAME
IUBMB Comments
alcohol:oxygen oxidoreductase
The enzymes from the fungi Candida methanosorbosa and several Basidiomycetes species contain an FAD cofactor [1,3]. The enzyme from the phytopathogenic fungi Colletotrichum graminicola and Colletotrichum gloeosporioides utilize a mononuclear copper-radical mechanism [4]. The enzyme acts on primary alcohols and unsaturated alcohols, and has much lower activity with branched-chain and secondary alcohols.
CAS REGISTRY NUMBER
COMMENTARY hide
9073-63-6
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
additional information
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enzyme is able to oxidize 5-(hydroxymethyl)furfural, yielding furan-2,5-dicarbaldehyde, reaction of EC 1.1.3.47
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SOURCE TISSUE
ORGANISM
UNIPROT
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LITERATURE
SOURCE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
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LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
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fabrication of a self-powered ethanol biosensor comprising a beta-NAD+-dependent alcohol dehydrogenase bioanode and a bienzymatic alcohol oxidase and horseradish peroxidase biocathode. beta-NAD+ is regenerated by means of a toluidine blue modified redox polymer. The biofuel cell exhibits an open-circuit voltage of approximately 660 mV and can be used as self-powered device for the determination of the ethanol content in liquor
biotechnology
in the core promoter and 5' untranslated region of the gene, mutations in the TATA box motif, regions downstream of the transcription start site or next to the start codon in the 5' UTR have a significant effect on expression. Mutations in most other regions are tolerated. These neutral core promoter positions, not affecting expression, can be exploited to introduce extrinsic sequence elements such as cloning sites and bacterial promoters
synthesis
the combination of alcohol oxidase and catalase is most effective in converting over 97% 5-hydroxymethylfurfural to 2,5-diformylfuran in 72 h
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cajnko, M.; Novak, U.; Grilc, M.; Likozar, B.
Enzymatic conversion reactions of 5-hydroxymethylfurfural (HMF) to bio-based 2,5-diformylfuran (DFF) and 2,5-furandicarboxylic acid (FDCA) with air Mechanisms, pathways and synthesis selectivity
Biotechnol. Biofuels
13
66
2020
Komagataella phaffii (P04842), Komagataella phaffii GS115 (P04842)
Manually annotated by BRENDA team
Portela, R.M.C.; Vogl, T.; Ebner, K.; Oliveira, R.; Glieder, A.
Pichia pastoris alcohol oxidase 1 (AOX1) core promoter engineering by high resolution systematic mutagenesis
Biotechnol. J.
13
e1700340
2018
Komagataella phaffii (F2QY27), Komagataella phaffii, Komagataella phaffii ATCC 76273 (F2QY27)
Manually annotated by BRENDA team
Ruff, A.; Pinyou, P.; Nolten, M.; Conzuelo, F.; Schuhmann, W.
A self-powered ethanol biosensor
ChemElectroChem
4
890-897
2017
Komagataella phaffii
-
Manually annotated by BRENDA team
Yang, D.; Reyes-De-Corcuera, J.
Increased activity of alcohol oxidase at high hydrostatic pressure
Enzyme Microb. Technol.
145
109751
2021
Komagataella phaffii
Manually annotated by BRENDA team
Buchholz-Afari, M.; Halalipour, A.; Yang, D.; Reyes-De-Corcuera, J.
Increased stability of alcohol oxidase under high hydrostatic pressure
J. Food Eng.
246
95-101
2019
Komagataella phaffii
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Manually annotated by BRENDA team