Information on EC 1.1.2.2 - mannitol dehydrogenase (cytochrome)

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The expected taxonomic range for this enzyme is: Acetobacteraceae

EC NUMBER
COMMENTARY
1.1.2.2
-
RECOMMENDED NAME
GeneOntology No.
mannitol dehydrogenase (cytochrome)
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
D-mannitol + a ferricytochrome c = D-fructose + a ferrocytochrome c + 2 H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Fructose and mannose metabolism
-
Pentose and glucuronate interconversions
-
SYSTEMATIC NAME
IUBMB Comments
D-mannitol:cytochrome-c 2-oxidoreductase
The enzyme from the bacterium Gluconobacter oxydans acts on polyols with a D-lyxo configuration, such as D-mannitol and D-sorbitol, with preference towards the former.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
polyol dehydrogenase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37250-78-5
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Komagataeibacter xylinus KU-1
strain KU-1
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acyclic D-erythro polyols + ferricytochrome c
acyclic D-erythro ketoses + ferrocytochrome c
show the reaction diagram
-
no other substances than D-mannitol and D-sorbitol are accepted as substrates
-
-
-
acyclic D-erythro polyols + ferricytochrome c
acyclic D-erythro ketoses + ferrocytochrome c
show the reaction diagram
-
activity found with erythritiol, ribitol, D-arabinitol, D-sorbitol and others
-
?
acyclic D-erythro polyols + ferricytochrome c
acyclic D-erythro ketoses + ferrocytochrome c
show the reaction diagram
-
activity found with erythritiol, ribitol, D-arabinitol, D-sorbitol and others
-
?
acyclic D-erythro polyols + ferricytochrome c
acyclic D-erythro ketoses + ferrocytochrome c
show the reaction diagram
Komagataeibacter xylinus KU-1
-
no other substances than D-mannitol and D-sorbitol are accepted as substrates
-
-
-
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
-
-
-
?
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
-
-
-
?
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
-
-
-
?
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
-
other hydrogen acceptors: phenol blue, ferricyanide, phenolindo-2,6-dichlorophenol, o-chlorophenolindo-2,6-dichlorophenol
-
?
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
-
other hydrogen acceptors: phenol blue, ferricyanide, phenolindo-2,6-dichlorophenol, o-chlorophenolindo-2,6-dichlorophenol
-
?
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
Komagataeibacter xylinus KU-1
-
-
-
?
D-sorbitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
-
-
-
?
D-sorbitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
-
-
-
-
-
D-sorbitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
-
-
-
?
D-sorbitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
Komagataeibacter xylinus KU-1
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
-
-
-
?
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
-
-
-
?
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
-
-
-
?
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
Komagataeibacter xylinus KU-1
-
-
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
phenazine methosulfate
-
suitable as electron acceptor
methylene blue
-
suitable as electron acceptor
additional information
-
other electron acceptors: phenol blue, ferricyanide, phenolindo-2,6-dichlorophenol, o-chlorophenolindo-2,6-dichlorophenol
-
additional information
-
no activity with NAD+ and NADP+
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
restores activity after inactivation with EDTA and storage
Mg2+
-
restores activity after inactivation with EDTA and storage
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Hg2+
-
strong inhibitor
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
17
-
D-Arabitol
-
-
0.098
-
D-mannitol
-
with phenanzine methosulfate as electron acceptor
31
-
D-mannitol
-
-
34
-
D-mannitol
-
-
20
-
D-sorbitol
-
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
140000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
trimer
-
alpha,beta,gamma 1 * 69000 + 1 * 51000 + 1 * 20000, SDS-PAGE
trimer
Komagataeibacter xylinus KU-1
-
alpha,beta,gamma 1 * 69000 + 1 * 51000 + 1 * 20000, SDS-PAGE
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
7
-
stable for 30 min at 30°C
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50
-
-
stable up to 50°C at pH 6
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
0°C, stable for several weeks, lost activity can be restored by adding Ca2+ or Mg2+
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE