Information on EC 1.1.2.2 - mannitol dehydrogenase (cytochrome)

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The expected taxonomic range for this enzyme is: Acetobacteraceae

EC NUMBER
COMMENTARY
1.1.2.2
-
RECOMMENDED NAME
GeneOntology No.
mannitol dehydrogenase (cytochrome)
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
D-mannitol + a ferricytochrome c = D-fructose + a ferrocytochrome c + 2 H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Fructose and mannose metabolism
-
Pentose and glucuronate interconversions
-
SYSTEMATIC NAME
IUBMB Comments
D-mannitol:cytochrome-c 2-oxidoreductase
The enzyme from the bacterium Gluconobacter oxydans acts on polyols with a D-lyxo configuration, such as D-mannitol and D-sorbitol, with preference towards the former.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
polyol dehydrogenase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37250-78-5
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Gluconacetobacter xylinus
strain KU-1
-
-
Manually annotated by BRENDA team
Gluconacetobacter xylinus KU-1
strain KU-1
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acyclic D-erythro polyols + ferricytochrome c
acyclic D-erythro ketoses + ferrocytochrome c
show the reaction diagram
Gluconacetobacter xylinus
-
no other substances than D-mannitol and D-sorbitol are accepted as substrates
-
-
-
acyclic D-erythro polyols + ferricytochrome c
acyclic D-erythro ketoses + ferrocytochrome c
show the reaction diagram
-
activity found with erythritiol, ribitol, D-arabinitol, D-sorbitol and others
-
?
acyclic D-erythro polyols + ferricytochrome c
acyclic D-erythro ketoses + ferrocytochrome c
show the reaction diagram
-
activity found with erythritiol, ribitol, D-arabinitol, D-sorbitol and others
-
?
acyclic D-erythro polyols + ferricytochrome c
acyclic D-erythro ketoses + ferrocytochrome c
show the reaction diagram
Gluconacetobacter xylinus KU-1
-
no other substances than D-mannitol and D-sorbitol are accepted as substrates
-
-
-
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
Gluconacetobacter xylinus
-
-
-
?
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
-
-
-
?
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
-
-
-
?
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
-
other hydrogen acceptors: phenol blue, ferricyanide, phenolindo-2,6-dichlorophenol, o-chlorophenolindo-2,6-dichlorophenol
-
?
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
-
other hydrogen acceptors: phenol blue, ferricyanide, phenolindo-2,6-dichlorophenol, o-chlorophenolindo-2,6-dichlorophenol
-
?
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
Gluconacetobacter xylinus KU-1
-
-
-
?
D-sorbitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
Gluconacetobacter xylinus
-
-
-
?
D-sorbitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
-
-
-
-
-
D-sorbitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
-
-
-
?
D-sorbitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
Gluconacetobacter xylinus KU-1
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
Gluconacetobacter xylinus
-
-
-
?
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
-
-
-
?
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
-
-
-
?
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
Gluconacetobacter xylinus KU-1
-
-
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
cytochrome c
Gluconacetobacter xylinus
-
-
phenazine methosulfate
Gluconacetobacter xylinus
-
suitable as electron acceptor
methylene blue
-
suitable as electron acceptor
additional information
-
other electron acceptors: phenol blue, ferricyanide, phenolindo-2,6-dichlorophenol, o-chlorophenolindo-2,6-dichlorophenol
-
additional information
Gluconacetobacter xylinus
-
no activity with NAD+ and NADP+
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
restores activity after inactivation with EDTA and storage
Mg2+
-
restores activity after inactivation with EDTA and storage
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Hg2+
Gluconacetobacter xylinus
-
strong inhibitor
p-quinone
Gluconacetobacter xylinus
-
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
17
-
D-Arabitol
-
-
0.098
-
D-Mannitol
Gluconacetobacter xylinus
-
with phenanzine methosulfate as electron acceptor
31
-
D-Mannitol
-
-
34
-
D-Mannitol
-
-
20
-
D-Sorbitol
-
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.18
-
p-quinone
Gluconacetobacter xylinus
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
213
-
Gluconacetobacter xylinus
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
-
Gluconacetobacter xylinus
-
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3
8
Gluconacetobacter xylinus
-
-
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
40
-
Gluconacetobacter xylinus
-
-
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
60
Gluconacetobacter xylinus
-
-
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
140000
-
Gluconacetobacter xylinus
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
trimer
Gluconacetobacter xylinus
-
alpha,beta,gamma 1 * 69000 + 1 * 51000 + 1 * 20000, SDS-PAGE
trimer
Gluconacetobacter xylinus KU-1
-
alpha,beta,gamma 1 * 69000 + 1 * 51000 + 1 * 20000, SDS-PAGE
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
7
Gluconacetobacter xylinus
-
stable for 30 min at 30°C
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50
-
Gluconacetobacter xylinus
-
stable up to 50°C at pH 6
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
0°C, stable for several weeks, lost activity can be restored by adding Ca2+ or Mg2+
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-
Gluconacetobacter xylinus
-