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Information on EC 1.1.1.B40 - 11beta-hydroxysteroid dehydrogenase (NAD+) and Organism(s) Cavia porcellus and UniProt Accession Q6QLL4

for references in articles please use BRENDA:EC1.1.1.B40
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Cavia porcellus
UNIPROT: Q6QLL4 not found.
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The taxonomic range for the selected organisms is: Cavia porcellus
The enzyme appears in selected viruses and cellular organisms
Synonyms
11 beta-hydroxysteroid dehydrogenase, 11 beta-hydroxysteroid dehydrogenase type 2, 11beta-hsd type 2, 11beta-ohsd, 11beta-hsd 2, 11-beta hydroxysteroid dehydrogenase type 2, type 2 11beta-hydroxysteroid dehydrogenase, 11beta hydroxysteroid dehydrogenase type 2, 11beta-hydroxysteroid dehydrogenase type-2, nad(+)-dependent 11beta-dehydrogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11beta-HSD1
-
11beta-hydroxysteroid dehydrogenase type 1
-
corticosteroid 11-beta-dehydrogenase isozyme 1
-
11beta-OHSD
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an 11beta-hydroxysteroid + NAD+ = an 11-oxosteroid + NADH + H+
show the reaction diagram
the three-dimensional organization of the hydrophobic substrate binding pocket is responsible for species-specific variability of activity with different substrates, overview
SYSTEMATIC NAME
IUBMB Comments
11beta-hydroxysteroid:NAD+ 11-oxidoreductase
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
11-dehydrocorticosterone + NADPH
corticosterone + NADP+
show the reaction diagram
isozyme 11beta-HSD1
-
-
?
7-oxocholesterol + NADPH + H+
7beta-hydroxycholesterol + NADP+
show the reaction diagram
isozyme 11beta-HSD1, stereospecific reaction, very low activity
-
-
?
cortisone + NADPH + H+
cortisol + NADP+
show the reaction diagram
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NAD+
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADH
show the reaction diagram
-
i.e. cortisol
i.e. cortisone
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
11-dehydrocorticosterone + NADPH
corticosterone + NADP+
show the reaction diagram
isozyme 11beta-HSD1
-
-
?
cortisone + NADPH + H+
cortisol + NADP+
show the reaction diagram
isozyme 11beta-HSD1, activation
-
-
?
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NAD+
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADH
show the reaction diagram
-
i.e. cortisol
i.e. cortisone
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2'-hydroxyflavanone
-
abietic acid
-
carbenoxolone
-
chenodeoxycholic acid
-
glycyrrhetinic acid
-
3beta-hydroxy-11-oxo-18beta-olean-12-en-30-oic-acid
chenodeoxycholic acid
-
IC50: 0.513 mM
cholic acid
-
IC50: 3.529 mM
Ethacrynic acid
-
IC50: 0.452 mM
Furosemide
-
IC50: 0.059 mM, competitive
naringenin
-
IC50: 0.336 mM
naringin
-
IC50: 2.373 mM
Phenylbutazone
-
IC50: 1.358 mM
sistosterol
-
IC50: 1.395 mM
stigmasterol
-
IC50: 1.968 mM
additional information
inhibitory effects in intact cells and IC50 values, overview, structure-based pharmacophore models for isozyme 11beta-HSD1 inhibitors using crystal structure 1XU7, 1XU9, and 2 BEL
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00043
11-Dehydrocorticosterone
pH 7.4, 37°C, recombinant isozyme 11beta-HSD1
0.00064
cortisone
pH 7.4, 37°C, recombinant isozyme 11beta-HSD1
0.008
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione
-
i.e. cortisol
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0049
2'-hydroxyflavanone
pH 7.4, 37°C, isozyme 11beta-HSD1, inhibition of cortisol oxidation
0.007
abietic acid
pH 7.4, 37°C, isozyme 11beta-HSD1, inhibition of cortisol oxidation
0.00023
carbenoxolone
pH 7.4, 37°C, isozyme 11beta-HSD1, inhibition of cortisol oxidation
0.0021
chenodeoxycholic acid
pH 7.4, 37°C, isozyme 11beta-HSD1, inhibition of cortisol oxidation
0.031
flavanone
pH 7.4, 37°C, isozyme 11beta-HSD1, inhibition of cortisol oxidation
0.00033
glycyrrhetinic acid
pH 7.4, 37°C, isozyme 11beta-HSD1, inhibition of cortisol oxidation
0.000025
T0504
pH 7.4, 37°C, isozyme 11beta-HSD1, inhibition of cortisol oxidation
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.254
3beta-hydroxy-11-oxo-18beta-olean-12-en-30-oic-acid
Cavia porcellus
-
IC50: 0.254 mM
0.513
chenodeoxycholic acid
Cavia porcellus
-
IC50: 0.513 mM
3.529
cholic acid
Cavia porcellus
-
IC50: 3.529 mM
0.452
Ethacrynic acid
Cavia porcellus
-
IC50: 0.452 mM
0.059
Furosemide
Cavia porcellus
-
IC50: 0.059 mM, competitive
0.336
naringenin
Cavia porcellus
-
IC50: 0.336 mM
2.373
naringin
Cavia porcellus
-
IC50: 2.373 mM
1.358
Phenylbutazone
Cavia porcellus
-
IC50: 1.358 mM
1.395
sistosterol
Cavia porcellus
-
IC50: 1.395 mM
1.968
stigmasterol
Cavia porcellus
-
IC50: 1.968 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
isozyme 11beta-HSD1; gene HSD11B1, isozyme 11beta-HSD1
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
cortex
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DHI1_CAVPO
300
1
33226
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32000
x * 32000, recombinant isozyme 11beta-HSD1, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 32000, recombinant isozyme 11beta-HSD1, SDS-PAGE
additional information
structure molecular modeling
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of isozyme 11beta-HSD1 in HEK-293 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Zhang, Y.D.; Lorenzo, B.; Reidenberg, M.M.
Inhibition of 11beta-hydroxysteroid dehydrogenase obtained from guinea pig kidney by furosemide, naringenin and some other compounds
J. Steroid Biochem. Mol. Biol.
49
81-85
1994
Cavia porcellus
Manually annotated by BRENDA team
Arampatzis, S.; Kadereit, B.; Schuster, D.; Balazs, Z.; Schweizer, R.A.; Frey, F.J.; Langer, T.; Odermatt, A.
Comparative enzymology of 11beta-hydroxysteroid dehydrogenase type 1 from six species
J. Mol. Endocrinol.
35
89-101
2005
Canis lupus familiaris, Cavia porcellus (Q6QLL4), Homo sapiens (P28845), Homo sapiens (P80365), Mesocricetus auratus (Q6R0J2), Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team