Information on EC 1.1.1.B35 - D-arabinose 1-dehydrogenase (NADP+)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Sulfolobus solfataricus

EC NUMBER
COMMENTARY hide
1.1.1.B35
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
D-arabinose 1-dehydrogenase (NADP+)
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-arabinose + NADP+ = D-arabinono-1,4-lactone + NADPH + H+
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
degradation of pentoses
-
-
SYSTEMATIC NAME
IUBMB Comments
D-arabinose:NADP+ 1-oxidoreductase
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-arabinose + NAD+
D-arabinono-1,4-lactone + NADH + H+
show the reaction diagram
D-arabinose + NADP+
D-arabinono-1,4-lactone + NADPH + H+
show the reaction diagram
D-ribose + NADP+
D-ribonolactone + NADPH
show the reaction diagram
L-fucose + NADP+
L-fuconolactone + NADPH
show the reaction diagram
L-galactose + NADP+
L-galactonolactone + NADPH
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-arabinose + NADP+
D-arabinono-1,4-lactone + NADPH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
-
the catalytic efficiency of NADP+ in combination with D-arabinose is 36-fold higher than that of NAD+
NADP+
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.4
D-arabinose
-
pH 7.5, 70C, determined with 0.4 mM NADP+
0.22
L-fucose
-
pH 7.5, 70C, determined with 0.4 mM NADP+
1.25
NAD+
-
pH 7.5, 70C, determined with 10 mM D-arabinose
0.038 - 0.04
NADP+
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
23.8
D-arabinose
Sulfolobus solfataricus
-
pH 7.5, 80C
17.7
D-ribose
Sulfolobus solfataricus
-
pH 7.5, 80C
26.8
L-fucose
Sulfolobus solfataricus
-
pH 7.5, 80C
17.7
L-galactose
Sulfolobus solfataricus
-
pH 7.5, 80C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10.6
D-arabinose
Sulfolobus solfataricus
-
pH 7.5, 70C, determined with 0.4 mM NADP+
565
67
L-fucose
Sulfolobus solfataricus
-
pH 7.5, 70C, determined with 0.4 mM NADP+
717
14.5
NAD+
Sulfolobus solfataricus
-
pH 7.5, 70C, determined with 10 mM D-arabinose
7
525
NADP+
Sulfolobus solfataricus
-
pH 7.5, 70C, determined with 10 mM D-arabinose
10
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.1 - 9.1
-
pH 7.1: about 40% of maximal activity, pH 9.1: about 60% of maximal activity, pH 9.5: about 30% of maximal activity
7.3 - 9.3
-
more than 50% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70 - 100
-
70C: about 40% of maximal activity, 100C: about 90% of maximal activity
74 - 100
-
more than 50% of maximal activity
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37291
-
4 * 37291, calculated from sequence
149700
-
mass spectrometry
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
tetramer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
the structure is solved to a resolution of 1.80 A by single-wavelength anomalous diffraction and phased using the two endogenous zinc ions per subunit. The structure reveals a catalytic and cofactor binding domain
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
85
-
half-life: 42 min
90
-
half-life: 26 min
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-