Information on EC 1.1.1.91 - aryl-alcohol dehydrogenase (NADP+)

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.1.91
-
RECOMMENDED NAME
GeneOntology No.
aryl-alcohol dehydrogenase (NADP+)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an aromatic alcohol + NADP+ = an aromatic aldehyde + NADPH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
aryl-alcohol:NADP+ oxidoreductase
Also acts on some aliphatic aldehydes, but cinnamaldehyde was the best substrate found.
CAS REGISTRY NUMBER
COMMENTARY hide
37250-27-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
wheatgrass
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
alfalfa
-
-
Manually annotated by BRENDA team
American Type Culture Collection No.607
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
JCM3016
-
-
Manually annotated by BRENDA team
JCM3016
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
rye
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
tomato
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Triticale turgidocereale
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(E)-oct-2-enal + NADPH + H+
trans-2-octen-1-ol + NADP+
show the reaction diagram
-
-
-
?
(R)-1-phenylethanol + NAD+
1-phenylacetaldehyde + NADH + H+
show the reaction diagram
(R)-2-octanol + NADPH + H+
?
show the reaction diagram
(S)-1-phenylethanol + NADP+
1-phenylacetaldehyde + NADPH + H+
show the reaction diagram
(S)-2-octanol + NADPH + H+
?
show the reaction diagram
1-butanol + NADP+
butyraldehyde + NADPH + H+
show the reaction diagram
56% activity compared to crotyl alcohol
-
-
?
1-propanol + NADP+
propionaldehyde + NADPH + H+
show the reaction diagram
17.4% activity compared to crotyl alcohol
-
-
?
2,4-dimethoxybenzyl alcohol + NADP+
2,4-dimethoxybenzyl aldehyde + NADPH + H+
show the reaction diagram
-
-
-
?
2-fluorobenzaldehyde + NADPH
2-fluorobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 27% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
2-hydroxyacetophenone + NADPH + H+
(S)-1,2-phenylethanediol + NADP+
show the reaction diagram
-
-
-
?
2-methoxybenzaldehyde + NADPH
2-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 58% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
2-methyl-propanol + NADPH + H+
?
show the reaction diagram
2-nitrobenzaldehyde + NADPH
2-nitrobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 57% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
3,4,5-trimethoxybenzaldehyde + NADPH
3,4,5-trimethoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
3,4-dihydroxybenzaldehyde + NADPH
3,4-dihydroxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
3,4-dimethoxybenzaldehyde + NADH
3,4-dimethoxybenzyl alcohol + NAD+
show the reaction diagram
-
-
-
-
r
3,4-dimethoxybenzaldehyde + NADPH
3,4-dimethoxybenzyl alcohol + NADP+
show the reaction diagram
3,4-dimethoxybenzyl alcohol + NADP+
?
show the reaction diagram
-
-
-
?
3,4-dimethoxybenzyl alcohol + NADP+
veratraldehyde + NADPH + H+
show the reaction diagram
-
-
-
r
3,5-dimethoxybenzaldehyde + NADPH
3,5-dimethoxybenzyl alcohol + NADP+
show the reaction diagram
3-chlorobenzaldehyde + NADPH
3-chlorobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 73% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
3-fluorobenzaldehyde + NADPH
3-fluorobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 69% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
3-hydroxy-4-methoxy-benzaldehyde + NADPH
3-hydroxy-4-methoxy-benzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 17% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
3-hydroxy-4-methoxybenzaldehyde + NADPH + H+
3-hydroxy-4-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
3-methoxybenzaldehyde + NADPH
3-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 117% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
3-methyl-butanol + NADPH + H+
?
show the reaction diagram
-
-
-
-
?
3-nitrobenzaldehyde + NADPH
3-nitrobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 25% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
4-(hydroxymethyl)benzoic acid + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
4-benzyloxy-3-methoxybenzaldehyde + NADPH + H+
4-benzyloxy-3-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
4-chlorobenzaldehyde + NADPH
4-chlorobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 61% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
4-fluorobenzaldehyde + NADPH
4-fluorobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 29% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
4-hydroxy-3-methoxy-benzaldehyde + NADPH
4-hydroxy-3-methoxy-benzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 19% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
4-hydroxy-3-methoxybenzaldehyde + NADPH + H+
4-hydroxy-3-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
4-hydroxybenzaldehyde + NADPH
4-hydroxybenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 34% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
4-hydroxybenzaldehyde + NADPH + H+
4-hydroxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
4-methoxybenzaldehyde + NADPH
4-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 110% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
4-methoxybenzaldehyde + NADPH + H+
4-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
4-methoxybenzyl alcohol + NADP+
4-methoxybenzaldehyde + NADPH
show the reaction diagram
4-nitrobenzaldehyde + NADPH
4-nitrobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 31% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
5-(hydroxymethyl)-2-furaldehyde + NADPH + H+
(furan-2,5-diyl)dimethanol + NADP+
show the reaction diagram
-
relative activity: 36% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
show the reaction diagram
-
-
-
-
r
acetophenone + NADPH + H+
?
show the reaction diagram
-
-
-
r
alpha-ionone + NADPH + H+
?
show the reaction diagram
-
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
show the reaction diagram
benzaldehyde + NADPH + H+
benzyl alcohol + NAD+
show the reaction diagram
reduction of benzaldehyde at pH 6.0 is NADPH-dependent while NADH does not yield detectable activity under the same conditions
-
-
r
benzaldehyde + NADPH + H+
benzyl alcohol + NADP+
show the reaction diagram
highest reductive activity with benzaldehyde
-
-
r
benzil + NADPH + H+
benzoin + NADP+
show the reaction diagram
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADPH + H+
show the reaction diagram
oxidation of benzyl alcohol at pH 9.6 is NAD+-dependent while NADP+ did not yield detectable activity under the same conditions
-
-
r
benzyl alcohol + NADP+
benzaldehyde + NADPH
show the reaction diagram
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
show the reaction diagram
butanol + NADPH + H+
?
show the reaction diagram
-
-
-
-
?
cinnamic alcohol + NADP+
cinnamic aldehyde + NADPH
show the reaction diagram
coniferyl alcohol + NADP+
coniferylaldehyde + NADPH + H+
show the reaction diagram
crotonaldehyde + NADPH + H+
crotonyl alcohol + NADP+
show the reaction diagram
-
-
-
r
crotonyl alcohol + NADP+
crotonaldehyde + NADPH + H+
show the reaction diagram
highest oxidative activity with crotyl alcohol
-
-
r
decanal + NADPH
decanol + NADP+
show the reaction diagram
-
relative activity: 2% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
heptaldehyde + NADPH
?
show the reaction diagram
-
relative activity: 26% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
hexanal + NADPH
hexanol + NADP+
show the reaction diagram
-
relative activity: 47% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
m-bromobenzaldehyde + NADPH + H+
m-bromobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
m-chlorobenzaldehyde + NADPH + H+
m-chlorobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
m-fluorobenzaldehyde + NADPH + H+
m-fluorobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
m-hydroxybenzaldehyde + NADPH + H+
m-hydroxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
m-nitrobenzaldehyde + NADPH
m-nitrobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
n-butanol + NADP+
n-butanal + NADPH
show the reaction diagram
-
-
-
-
r
n-pentanol + NADP+
n-pentanal + NADPH
show the reaction diagram
-
-
-
-
r
n-propanol + NADP+
n-propanal + NADPH
show the reaction diagram
-
-
-
-
r
nonanal + NADPH
nonanol + NADP+
show the reaction diagram
-
relative activity: 3% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
o-bromobenzaldehyde + NADPH + H+
o-bromobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
o-chlorobenzaldehyde + NADPH + H+
o-chlorobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
o-fluorobenzaldehyde + NADPH + H+
o-fluorobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
octanal + NADPH
octanol + NADP+
show the reaction diagram
-
relative activity: 37% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
p-aminobenzyl alcohol + NADP+
p-aminobenzaldehyd + NADPH
show the reaction diagram
-
-
-
-
r
p-bromobenzaldehyde + NADPH + H+
p-bromobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
p-chlorobenzaldehyde + NADPH + H+
p-chlorobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
p-fluorobenzaldehyde + NADPH + H+
p-fluorobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
p-hydroxybenzaldehyde + NADPH + H+
p-hydroxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
p-methylbenzaldehyde + NADPH
p-methylbenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
pentanal + NADPH
pentanol + NADP+
show the reaction diagram
-
relative activity: 8% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
phenylacetaldehyde + NADPH
phenylethanol + NADP+
show the reaction diagram
-
relative activity: 109 (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
phenylacetaldehyde + NADPH + H+
phenylethanol + NADP+
show the reaction diagram
-
-
-
-
?
rac-4-methyl-2-pentanol + NADPH + H+
?
show the reaction diagram
-
-
-
-
?
rac-butan-2,3-diol + NADPH + H+
?
show the reaction diagram
-
-
-
-
?
salicyl alcohol + NADP+
p-hydroxybenzaldehyde + NADPH
show the reaction diagram
-
i.e. p-hydroxybenzyl alcohol
-
-
r
trans-4-phenyl-3-buten-2-one + NADPH + H+
?
show the reaction diagram
-
-
-
r
trans-cinnamaldehyde + NADPH
?
show the reaction diagram
-
relative activity: 39% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
vanillin + NADPH + H+
vanillic alcohol + NADP+
show the reaction diagram
-
-
-
r
veratraldehyde + NADPH + H+
3,4-dimethoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
r
veratryl alcohol + NADP+
veratraldehyde + NADPH
show the reaction diagram
-
i.e. 3,4-dimethoxybenzyl alcohol
-
-
r
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
benzyl alcohol + NADP+
benzaldehyde + NADPH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
NADPH
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
113% activity at 1 mM
Zn2+
dependent on
additional information
Ca2+, Na+, Mg2+, Mn2+, and K+ have no significant effect on the enzyme activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
3,4-dimethoxybenzaldehyde
-
-
3,4-dimethoxybenzyl alcohol
-
-
3,5-dimethoxybenzaldehyde
-
-
3-hydroxy-4-methoxy-benzaldehyde
-
-
4-hydroxy-3-methoxybenzaldehyde
-
-
5,5'-dithio-bis(2-nitrobenzoic acid)
-
-
5-(hydroxymethyl)-2-furaldehyde
-
-
Ag+
complete inhibition at 1 mM
AgNO3
Al3+
13.5% inhibition at 1 mM
Co2+
80.5% inhibition at 1 mM
Cu2+
complete inhibition at 1 mM
diphenyl hydantoin
-
-
Fe2+
39.7% inhibition at 1 mM
iodoacetate
94.5% residual activity at 10 mM
Iodosobenzoate
-
-
p-chloromercuribenzoate
trans-cinnamaldehyde
-
-
Zn2+
99.5% inhibition at 1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetone
125% activity at 20% (v/v) acetone
dithiothreitol
123% activity at 1 mM
EDTA
110% activity at 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.5
(R)-1-phenylethanol
wild-type, pH 8.3, 30C
1.2 - 17.6
(S)-1-phenylethanol
7.1 - 7.7
(S)-2-octanol
0.012
3,4-dimethoxybenzaldehyde
-
pH 6.1, 30C
0.37
3,4-dimethoxybenzyl alcohol
-
pH 6.1, 30C
0.022
3,5-dimethoxybenzaldehyde
-
pH 6.1, 30C
1.4
3-hydroxy-4-methoxy-benzaldehyde
-
pH 6.1, 30C
0.55
3-phenylpropionaldehyde
-
-
1.4
4-Hydroxy-3-methoxy-benzaldehyde
-
pH 6.1, 30C
25
4-hydroxy-3-methoxy-benzyl alcohol
-
pH 6.1, 30C
0.09 - 0.5
4-methoxybenzaldehyde
1.7
4-methoxybenzyl alcohol
-
-
0.27
5-(hydroxymethyl)-2-furaldehyde
-
pH 6.1, 30C
1.7
acetaldehyde
-
-
0.34 - 1.7
benzaldehyde
0.057 - 14
benzyl alcohol
0.22
cinnamic aldehyde
-
-
0.55
coniferyl alcohol
-
-
0.14
coniferyl aldehyde
-
-
3.3
crotonaldehyde
at pH 8.0 and 55C
9.1
crotonyl alcohol
at pH 8.0 and 55C
0.16
m-bromobenzaldehyde
-
-
0.1
m-chlorobenzaldehyde
-
-
0.42
m-fluorobenzaldehyde
-
-
1.1
m-hydroxybenzaldehyde
-
-
0.21
m-nitrobenzaldehyde
-
-
0.8 - 3.1
NAD+
0.0026 - 0.22
NADH
0.024 - 2.3
NADP+
0.017 - 0.1
NADPH
0.25
o-bromobenzaldehyde
-
-
0.2
o-chlorobenzaldehyde
-
-
0.27
o-fluorobenzaldehyde
-
-
0.05
o-nitrobenzaldehyde
-
-
0.04
p-aminobenzyl alcohol
-
-
0.22
p-bromobenzaldehyde
-
-
0.34
p-chlorobenzaldehyde
-
-
0.26
p-fluorobenzaldehyde
-
-
0.09
p-Hydroxybenzaldehyde
-
-
0.3
p-methylbenzaldehyde
-
-
0.04
p-nitrobenzaldehyde
-
-
0.53
phenylacetaldehyde
-
pH 6.1, 30C
3.4
trans-cinnamaldehyde
-
pH 6.1, 30C
0.25
veratraldehyde
at pH 6.0 temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0047
(R)-1-phenylethanol
Ralstonia sp.
C0IR58
wild-type, pH 8.3, 30C
0.0115 - 0.783
(S)-1-phenylethanol
4.3 - 13.7
(S)-2-octanol
8.833
3,4-dimethoxybenzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
2.55
3,4-dimethoxybenzyl alcohol
Phanerochaete chrysosporium
-
pH 6.1, 30C
9.83
3,5-dimethoxybenzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
3.83
3-hydroxy-4-methoxy-benzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
3.33
4-Hydroxy-3-methoxy-benzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
4.33
4-hydroxy-3-methoxy-benzyl alcohol
Phanerochaete chrysosporium
-
pH 6.1, 30C
8.16
4-methoxybenzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
2.93
5-(hydroxymethyl)-2-furaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
7.16 - 360
benzaldehyde
93.3
benzyl alcohol
Yokenella sp. WZY002
W6CX26
at pH 6.5 and 65C
405
crotonaldehyde
Yokenella sp. WZY002
W6CX26
at pH 8.0 and 55C
101
crotonyl alcohol
Yokenella sp. WZY002
W6CX26
at pH 8.0 and 55C
0.022 - 0.08
NAD+
2
NADH
Phanerochaete chrysosporium
-
pH 6.1, 30C
0.00003 - 433
NADP+
11.33
NADPH
Phanerochaete chrysosporium
-
pH 6.1, 30C
11.16
phenylacetaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
11.16
trans-cinnamaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0047
(R)-1-phenylethanol
Ralstonia sp.
C0IR58
wild-type, pH 8.3, 30C
6838
0.00115 - 0.65
(S)-1-phenylethanol
4333
0.6 - 1.8
(S)-2-octanol
9492
733
3,4-dimethoxybenzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
2637
6.8
3,4-dimethoxybenzyl alcohol
Phanerochaete chrysosporium
-
pH 6.1, 30C
2814
450
3,5-dimethoxybenzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
7440
2.66
3-hydroxy-4-methoxy-benzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
19772
2.33
4-Hydroxy-3-methoxy-benzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
12368
0.166
4-hydroxy-3-methoxy-benzyl alcohol
Phanerochaete chrysosporium
-
pH 6.1, 30C
42614
90
4-methoxybenzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
1016
10
5-(hydroxymethyl)-2-furaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
19771
5 - 899
benzaldehyde
146
6.6
benzyl alcohol
Yokenella sp. WZY002
W6CX26
at pH 6.5 and 65C
260
122
crotonaldehyde
Yokenella sp. WZY002
W6CX26
at pH 8.0 and 55C
1529
11
crotonyl alcohol
Yokenella sp. WZY002
W6CX26
at pH 8.0 and 55C
105743
0.007 - 0.073
NAD+
7
10
NADH
Phanerochaete chrysosporium
-
pH 6.1, 30C
8
0.0001 - 720
NADP+
10
283
NADPH
Phanerochaete chrysosporium
-
pH 6.1, 30C
5
11.16
phenylacetaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
769
3.33
trans-cinnamaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
2225
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.4
3,4-dimethoxybenzaldehyde
-
pH 6.1, 30C
165
3,4-dimethoxybenzyl alcohol
-
pH 6.1, 30C
2.1
3,5-dimethoxybenzaldehyde
-
pH 6.1, 30C
2.3
3-hydroxy-4-methoxy-benzaldehyde
-
pH 6.1, 30C
5.1
4-hydroxy-3-methoxybenzaldehyde
-
pH 6.1, 30C
136
5-(hydroxymethyl)-2-furaldehyde
-
pH 6.1, 30C
81
benzaldehyde
-
pH 6.1, 30C
3.5
trans-cinnamaldehyde
-
pH 6.1, 30C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
with veratraldehyde as substrate
6.4
-
reduction activity
8
for crotonyl alcohol oxidation
8.8
-
alcohol oxidation
10.4
-
oxidation activity
11
-
for the oxidation reaction
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 10
at pH 8.0 and 10.0 the enzyme retains 62% activity, while the enzyme retains 13% activity at pH 4.0
6.2 - 7.2
-
in imidazole buffer
7 - 8
-
in phosphate buffer, activity of the enzyme is only one-half that shown in imidazole buffer
7.9 - 8.5
-
in Tris buffer, activity is about 40% as in imidazole buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
isoform SDR A5
50
-
isoform SDR B3
55
for crotonyl alcohol oxidation
65
for crotonaldehyde reduction
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 50
-
by raising the temperature of the incubation mixture a linear increase of the reaction up to 55C is noted, but the reaction over 45C stops after 60-75 s
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47000
-
4 * 47000, SDS-PAGE
280000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
-
4 * 47000, SDS-PAGE
homodimer
2 * 36411, calculated from amino acid sequence; 2 * 39000, SDS-PAGE
monomer
1 * 39000, SDS-PAGE; 1 * 40580, MALDI-TOF mass spectrometry
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystal structure of the apoenzyme as well as its NADP+-bound state with resolutions down to 2.8 A. ADH displays a homotetrameric quaternary structure that can be described as a dimer of homodimers while in each subunit a seven-stranded parallel beta-sheet, flanked by three alpha-helices on each side, forms a Rossmann fold-type dinucleotide binding domain. NADP+ specificity is largely governed by the residues Asn15, Gly37, Arg38, and Arg39
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55 - 65
the enzyme shows moderate thermostability with a half-life of 6.2 h at 55C and 1.5 h at 60C. The activity loss at 65C is relatively rapid, and the enzyme retains 30.8% of the initial activity after 30 min of heat treatment
58
the enzyme's half-life of deactivation at 58C is 4.2 min. There is no detectable enzyme activity when it is heated above 60C
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
DMSO
the enzyme retains 87.5% of the initial activity after 24 h of incubation with 20% (v/v) dimethyl sulfoxide and also remains active in 40% (v/v)
Ethanol
the enzyme retains more than 49% of the initial activity in 20% (v/v) ethanol and also remains active in 40% (v/v)
isopropanol
the enzyme retains more than 49% of the initial activity in 20% (v/v) isopropanol and also remains active in 40% (v/v)
Methanol
the enzyme retains more than 49% of the initial activity in 20% (v/v) methanol and also remains active in 40% (v/v)
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0C, potassium phosphate buffer pH 7.5, 1 mM mercaptoethanol, 2 weeks, no loss of activity
-
4C, 10 mM Tris/HCl pH 7.5, 6 days, 95% loss of activity
-
4C, 10 mM Tris/HCl pH 7.5, one month, 15% loss of activity
-
all buffers, standard buffer Tris-HCl , 5 mM mercaptoethanol and sucrose, 2.5 g/10 ml, in the final preparation, 2 weeks, little loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DEAE Sepharose column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration
Ni-NTA column chromatography
Ni-Sepharose column chromatography
-
using Ni-NTA chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a His-tagged fusion protein
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Top10 cells
-
expressed in Saccharomyces cerevisiae
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G37D
mutation in NADP+-binding site, strong decrease in activity with NADP+
N15G/G37D/R38V/R39S
mutation in NADP+-binding site, strong decrease in activity with NADP+
N15G/G37D/R38V/R39S/A86N/S88A
mutation in NADP+-binding site, strong decrease in activity with NADP+
G37D
-
mutation in NADP+-binding site, strong decrease in activity with NADP+
-
N15G/G37D/R38V/R39S
-
mutation in NADP+-binding site, strong decrease in activity with NADP+
-
N15G/G37D/R38V/R39S/A86N/S88A
-
mutation in NADP+-binding site, strong decrease in activity with NADP+
-
Show AA Sequence (304 entries)
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