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Information on EC 1.1.1.6 - glycerol dehydrogenase and Organism(s) Schizosaccharomyces pombe and UniProt Accession O13702

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EC Tree
     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.6 glycerol dehydrogenase
IUBMB Comments
Also acts on propane-1,2-diol.
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This record set is specific for:
Schizosaccharomyces pombe
UNIPROT: O13702
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Word Map
The taxonomic range for the selected organisms is: Schizosaccharomyces pombe
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Synonyms
gldh, glycerol dehydrogenase, tmglydh, nad-linked glycerol dehydrogenase, glycerol:nad+ 2-oxidoreductase, glycerol nad 2-oxidoreductase, nad+-dependent glycerol dehydrogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycerol dehydrogenase
-
dehydrogenase, glycerol
-
-
-
-
GDH2
-
-
-
-
GLDH
-
-
-
-
glycerin dehydrogenase
-
-
-
-
glycerol NAD 2-oxidoreductase
-
-
-
-
glycerol:NAD+ 2-oxidoreductase
-
-
-
-
NAD-linked glycerol dehydrogenase
-
-
-
-
NAD-specific glycerol dehydrogenase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
glycerol:NAD+ 2-oxidoreductase
Also acts on propane-1,2-diol.
CAS REGISTRY NUMBER
COMMENTARY hide
249285-11-8
-
9028-14-2
this number also refers to EC 1.1.99.22
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glycerol + NAD+
glycerone + NADH + H+
show the reaction diagram
no activity detected with NADP+
-
-
?
1,2,3-butanetriol + NAD+
1,3-dihydroxybutane-2-one + NADH
show the reaction diagram
-
-
-
?
1,2-butanediol + NAD+
1-hydroxybutane-2-one + NADH
show the reaction diagram
-
-
-
r
1,2-propanediol + NAD+
hydroxyacetone + NADH
show the reaction diagram
-
-
-
r
2,3-butanediol + NAD+
3-hydroxybutane-2-one + NADH
show the reaction diagram
-
-
-
r
DL-glyceraldehyde + NAD+
3-hydroxypyruvaldehyde + NADH
show the reaction diagram
-
-
-
?
ethanediol + NAD+
glycolaldehyde + NADH
show the reaction diagram
-
-
-
?
glycerol + NAD+
dihydroxyacetone + NADH + H+
show the reaction diagram
methylglyoxal + NADH
lactaldehyde + NAD+
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glycerol + NAD+
dihydroxyacetone + NADH + H+
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
-
increases activity significantly
Zn2+
-
increases activity
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
-
dithiothreitol
-
-
Li+
-
competitive, forward reaction
Na+
-
competitive, forward reaction
NADP+
-
inhibits oxidation of glycerol
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
iodoacetamide
-
at 1 mM 140% of the activity without activator, at 10 mM 220% of the activity without activator
N-ethylmaleimide
-
at 0.1 mM 145% of the activity without activator
p-chloromercuribenzoic acid
-
at 0.1 mM 133% of the activity without activator
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.06
dihydroxyacetone
-
pH 6.0
0.5
glycerol
-
pH 10.0
0.13
NAD+
-
pH 10.0
0.12
NADH
-
pH 6.0
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.01
wild type, crude cell extract, pH not specified in the publication, temperature not specified in the publication
0.049
overexpressing cells, crude cell extract, pH not specified in the publication, temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11 - 12
-
oxidation of glycerol
5 - 6
-
reduction of dihydroxyacetone
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9
-
pH 5.5: about 70% of maximal activity, pH 9.0: about 45% of maximal activity, reduction of dihydroxyacetone
9 - 12
-
pH 9.0: about 45% of maximal activity, pH 11-12: maximal activity, oxidation of glycerol
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
soluble, isolation from crude cell extract
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
400000
-
gel filtration
47000
-
8 * 47000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
octamer
-
8 * 47000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
10 min stable
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, long term storage
-
4°C, in 2 M (NH4)2SO4, 1 M glycerol, several months
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpressed in Schizosaccharomyces pombe
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
induced by glucose starvation, essential for glycerol assimilation
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kong, Y.C.; May, J.W.; Marshall, J.H.
Glycerol oxidation and triose reduction by pyridine nucleotide-linked enzyme in the fission yeast Schizosaccharomyces pombe
J. Gen. Microbiol.
131
1571-1579
1985
Schizosaccharomyces pombe, Schizosaccharomyces pombe 972h-
-
Manually annotated by BRENDA team
Marshall, J.H.; May, J.W.; Sloan, J.
Purification and properties of glycerol:NAD+ 2-oxidoreductase (glycerol dehydrogenase) from Schizosaccharomyces pombe
J. Gen. Microbiol.
131
1581-1588
1985
Schizosaccharomyces pombe
-
Manually annotated by BRENDA team
Matsuzawa, T.; Ohashi, T.; Hosomi, A.; Tanaka, N.; Tohda, H.; Takegawa, K.
The gld1+ gene encoding glycerol dehydrogenase is required for glycerol metabolism in Schizosaccharomyces pombe
Appl. Microbiol. Biotechnol.
87
715-727
2010
Schizosaccharomyces pombe (O13702), Schizosaccharomyces pombe, Schizosaccharomyces pombe ARC039 (O13702)
Manually annotated by BRENDA team