Information on EC 1.1.1.58 - tagaturonate reductase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.58
-
RECOMMENDED NAME
GeneOntology No.
tagaturonate reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-altronate + NAD+ = D-tagaturonate + NADH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-galacturonate degradation I
-
-
degradation of sugar acids
-
-
Pentose and glucuronate interconversions
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
D-altronate:NAD+ 3-oxidoreductase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9028-45-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
additional information
-
no activity with NADP+
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Fructuronate
-
-
mannonate
-
inhibitory for forward and reverse reaction
p-chloromercuribenzoate
additional information
-
products inhibition with all products in forward and reverse reaction
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.053 - 0.09
altronate
0.1
D-tagaturonate
-
-
0.075 - 0.11
NAD+
0.06 - 0.085
NADH
0.4
NADPH
-
-
0.35 - 0.67
tagaturonate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
68
-
crude extract
2730
-
purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.3
-
NADH oxidation
6.9 - 7
-
forward and reverse reaction
8.9
-
NAD+ reduction
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.3 - 8
-
pH 5.3: about 75% of activity maximum, pH 8.0: about 50% of activity maximum
6 - 8
-
at pH 6 and 8: about 50% of activity maximum
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 30
-
more active at 30C than at 20C
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
58.5
-
half-life: 10.5 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
altronate protects against heat denaturation
-
fructuronate does not protect against heat denaturation
-
mannonate protects against heat denaturation
-
NAD+ does not protect against heat denaturation
-
NADH protects against heat denaturation
-
NADPH protects against heat denaturation
-
tagaturonate does not protect against heat denaturation
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
3C or frozen at -10C, 1 month
-
4C or -20C, several months
-
4C, 10 mM sodium phosphate buffer, pH 7.0, 2 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
construction of a hybrid plasmid containing the Escherichia coli uxa B gene encoding for altronate oxidoreductase
-
expression in Saccharomyces cerevisiae
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
-
aldonate estimation
synthesis
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