Information on EC 1.1.1.58 - tagaturonate reductase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY
1.1.1.58
-
RECOMMENDED NAME
GeneOntology No.
tagaturonate reductase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
D-altronate + NAD+ = D-tagaturonate + NADH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
D-galacturonate degradation I
-
Metabolic pathways
-
Pentose and glucuronate interconversions
-
SYSTEMATIC NAME
IUBMB Comments
D-altronate:NAD+ 3-oxidoreductase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
altronate dehydrogenase
-
-
-
-
altronate oxidoreductase
-
-
-
-
altronic oxidoreductase
-
-
-
-
D-tagaturonate reductase
-
-
-
-
Tagaturonate dehydrogenase
-
-
-
-
Tagaturonate reductase
-
-
-
-
tagUAR
-
-
-
-
uxaB
A9QSF6
-
uxaB
Lactococcus lactis KF147
A9QSF6
-
-
CAS REGISTRY NUMBER
COMMENTARY
9028-45-9
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
ATCC 9637
-
-
Manually annotated by BRENDA team
Escherichia coli K12
K12
-
-
Manually annotated by BRENDA team
Lactococcus lactis KF147
-
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
-
-
-
r
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
-
-
-
r
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
-
-
-
r
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
-
-
-
r
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
-
-
-
r
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
-
-
-
r
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
-
-
-
r
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
-
-
-
r
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
-
involved in hexuronate pathway
-
r
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
Escherichia coli K12
-
-, involved in hexuronate pathway
-
r
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
Escherichia coli K12
-
-
-
r
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
Escherichia coli K12
-
-
-
r
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
-
-
-
r
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
-
-
-
r
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
-
-
-
r
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
-
-
-
r
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
-
-
-
r
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
-
-
-
r
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
-
-
-
r
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
Escherichia coli, Escherichia coli K12
-
involved in hexuronate pathway
-
r
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
Escherichia coli K12
-
-
-
r
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
Escherichia coli K12
-
-
-
r
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
NADPH
-
10% of the activity with NADH
additional information
-
no activity with NADP+
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
heavy metal ions are inhibitory, can be prevented by adding chelating agents
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Fructuronate
-
-
p-chloromercuribenzoate
-
slight inhibition, no inhibition below 10 mM
p-chloromercuribenzoate
-
non-competitive, Ki: 1 mM
mannonate
-
inhibitory for forward and reverse reaction
additional information
-
products inhibition with all products in forward and reverse reaction
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.053
-
altronate
-
-
0.09
-
altronate
-
-
0.1
-
D-Tagaturonate
-
-
0.088
-
NAD+
-
-
0.11
-
NAD+
-
-
0.06
-
NADH
-
-
0.4
-
NADPH
-
-
0.35
-
tagaturonate
-
-
0.67
-
tagaturonate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.2
-
-
crude extract
68
-
-
crude extract
328
-
-
purified enzyme
2730
-
-
purified enzyme
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.3
-
-
NADH oxidation
6.9
7
-
forward and reverse reaction
8.9
-
-
NAD+ reduction
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.3
8
-
pH 5.3: about 75% of activity maximum, pH 8.0: about 50% of activity maximum
6
8
-
at pH 6 and 8: about 50% of activity maximum
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
30
-
more active at 30C than at 20C
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
58.5
-
-
half-life: 10.5 min
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
altronate protects against heat denaturation
-
fructuronate does not protect against heat denaturation
-
mannonate protects against heat denaturation
-
NAD+ does not protect against heat denaturation
-
NADH protects against heat denaturation
-
NADPH protects against heat denaturation
-
tagaturonate does not protect against heat denaturation
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
3C or frozen at -10C, 1 month
-
4C or -20C, several months
-
4C, 10 mM sodium phosphate buffer, pH 7.0, 2 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
construction of a hybrid plasmid containing the Escherichia coli uxa B gene encoding for altronate oxidoreductase
-
expression in Saccharomyces cerevisiae
A9QSF6
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
A9QSF6
expression of Lactococcus lactis uxaB and uxaC genes encoding D-tagaturonate reductase and D-galacturonate isomerase, in Saccharomyces cerevisiae to investigate in vivo activity of the first steps of the D-galacturonate pathway. Although D-tagaturonate reductase could, in principle, provide an alternative means for re-oxidizing cytosolic NADH, addition of D-galacturonate does not restore anaerobic growth, possibly due to absence of a functional D-altronate exporter in Saccharomyces cerevisiae
additional information
Lactococcus lactis KF147
-
expression of Lactococcus lactis uxaB and uxaC genes encoding D-tagaturonate reductase and D-galacturonate isomerase, in Saccharomyces cerevisiae to investigate in vivo activity of the first steps of the D-galacturonate pathway. Although D-tagaturonate reductase could, in principle, provide an alternative means for re-oxidizing cytosolic NADH, addition of D-galacturonate does not restore anaerobic growth, possibly due to absence of a functional D-altronate exporter in Saccharomyces cerevisiae
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
analysis
-
aldonate estimation
analysis
Escherichia coli K12
-
aldonate estimation
-
synthesis
A9QSF6
expression of Lactococcus lactis uxaB and uxaC genes encoding D-tagaturonate reductase and D-galacturonate isomerase, in Saccharomyces cerevisiae to investigate in vivo activity of the first steps of the D-galacturonate pathway. Although D-tagaturonate reductase could, in principle, provide an alternative means for re-oxidizing cytosolic NADH, addition of D-galacturonate does not restore anaerobic growth, possibly due to absence of a functional D-altronate exporter in Saccharomyces cerevisiae
synthesis
Lactococcus lactis KF147
-
expression of Lactococcus lactis uxaB and uxaC genes encoding D-tagaturonate reductase and D-galacturonate isomerase, in Saccharomyces cerevisiae to investigate in vivo activity of the first steps of the D-galacturonate pathway. Although D-tagaturonate reductase could, in principle, provide an alternative means for re-oxidizing cytosolic NADH, addition of D-galacturonate does not restore anaerobic growth, possibly due to absence of a functional D-altronate exporter in Saccharomyces cerevisiae
-