Information on EC 1.1.1.57 - fructuronate reductase

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The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY
1.1.1.57
-
RECOMMENDED NAME
GeneOntology No.
fructuronate reductase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
D-mannonate + NAD+ = D-fructuronate + NADH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
D-fructuronate degradation
-
Metabolic pathways
-
Pentose and glucuronate interconversions
-
SYSTEMATIC NAME
IUBMB Comments
D-mannonate:NAD+ 5-oxidoreductase
Also reduces D-tagaturonate.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D-mannonate dehydrogenase
-
-
-
-
D-mannonate:NAD+ oxidoreductase
-
-
-
-
fructuronate reductase
-
-
-
-
mannonate oxidoreductase
-
-
-
-
mannonic dehydrogenase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9028-44-8
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
-
-
-
-
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
-
approximately equal activity towards both fructuronate and tagaturonate in the forward reaction, and towards D-mannonate and D-altronate in the reverse reaction
-
r
D-altronate + NAD+
D-tagaturonate + NADH
show the reaction diagram
-
D-tagaturonate and D-altronate are 20% as active as D-fructuronate and D-mannonate
-
r
D-glucuronate + NADH
L-gulonate + NAD+
show the reaction diagram
-
-
-
?
D-mannonate + NAD+
D-fructuronate + NADH
show the reaction diagram
-
-
-
r
D-mannonate + NAD+
D-fructuronate + NADH
show the reaction diagram
-
-
-
r
D-mannonate + NAD+
D-fructuronate + NADH
show the reaction diagram
-
-
-
r
D-mannonate + NAD+
D-fructuronate + NADH
show the reaction diagram
-
-
-
r
D-mannonate + NAD+
D-fructuronate + NADH
show the reaction diagram
-
-
-
r
D-mannonate + NAD+
D-fructuronate + NADH
show the reaction diagram
-
-
-
r
D-mannonate + NAD+
D-fructuronate + NADH
show the reaction diagram
-
involved in hexuronate pathway
-
r
D-mannonate + NAD+
D-fructuronate + NADH
show the reaction diagram
Escherichia coli K12
-
-, involved in hexuronate pathway
-
r
D-mannonate + NADP+
D-fructuronate + NADPH
show the reaction diagram
-
5% of activity compared with NAD+ as cofactor
-
r
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-mannonate + NAD+
D-fructuronate + NADH
show the reaction diagram
-
-
-
r
D-mannonate + NAD+
D-fructuronate + NADH
show the reaction diagram
-
-
-
r
D-mannonate + NAD+
D-fructuronate + NADH
show the reaction diagram
-
-
-
r
D-mannonate + NAD+
D-fructuronate + NADH
show the reaction diagram
-
-
-
r
D-mannonate + NAD+
D-fructuronate + NADH
show the reaction diagram
-
-
-
r
D-mannonate + NAD+
D-fructuronate + NADH
show the reaction diagram
Escherichia coli, Escherichia coli K12
-
involved in hexuronate pathway
-
r
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
NADP+
-
5% of activity wit NAD+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
ATP
-
competitive
ATP
-
non-competitive
D-Altronate
-
non-competitive
D-Altronate
-
-
D-Altronate
-
competitive at low concentrations; non-competitive
D-mannonate
-
non-competitive
NAD+
-
competitive
p-chloromercuribenzoate
-
complete inhibition at 0.03 mM
p-chloromercuribenzoate
-
complete inhibition at 0.005 mM, non-competive, Ki: 0.0001 mM
tagaturonate
-
competitive, inhibitory on forward reaction
L-gulonate
-
mixed non-competitive
additional information
-
product inhibition with all products in forward and reverse reaction
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.5
-
D-Fructuronate
-
-
1
-
D-Fructuronate
-
-
0.78
-
D-mannonate
-
-
1.7
-
D-mannonate
-
-
1.8
-
D-mannonate
-
-
0.9
-
D-Tagaturonate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.008
-
-
crude extract
0.5
-
-
purified enzyme
6
-
-
crude extract
26
-
-
crude extract
320
-
-
purified enzyme
770
-
-
purified enzyme
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.3
-
-
for NADH oxidation
8.4
-
-
for NAD reduction
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
-
-
unstable at lower pH
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
53
-
-
50% loss of activity within 10 min
53
-
-
loss of activity, NADH, NADPH, fructuronate, tagaturonate, NAD+, mannonate and altronate protect against inactivation
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
rapid loss of activity upon dialysis and mild heating
-
stabilized by 1 mM cysteine at pH 7
-
unstable on freezing and thawing
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
3°C, pH 7, 1 mM cysteine, stable for 1-2 weeks
-
4°C, 10 mM sodium phosphate buffer, pH 7, no loss of activity of the purified enzyme within 3 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE