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The taxonomic range for the selected organisms is: Nicotiana tabacum
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
ntred-1, allyl-adh, allyl-alcohol dehydrogenase,
more
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allyl-alcohol:NADP+ oxidoreductase
Also acts on saturated primary alcohols.
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(R)-pulegone + NADP+
(1R,4R)-isomenthone + (1R,4S)-menthone + NADPH + H+
recombinant enzyme
reaction is not stereospecific as with the native enzyme
-
?
(R)-pulegone + NADP+
(1R,4R)-isomenthone + NADPH + H+
native enzyme
-
-
?
(S)-pulegone + NADP+
(1S,4S)-isomenthone + (1S,4R)-menthone + NADPH + H+
recombinant enzyme
-
-
?
allyl alcohol + NADP+
acrolein + NADPH + H+
-
-
-
?
(2S,4S)-carveol + NADP+
(S)-carvone + NADPH
-
-
-
r
(S)-carvone + NADPH
(2S,4S)-carveol + NADP+
-
-
-
r
allyl alcohol + NADP+
acrolein + NADPH
additional information
?
-
allyl alcohol + NADP+
acrolein + NADPH
-
-
-
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r
allyl alcohol + NADP+
acrolein + NADPH
-
specific for the (S)-isomer of secondary allylic alcohols, several stereomers tested for activity in both reaction directions
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-
r
additional information
?
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(R)-carvone, (S)-carvone, (1S,5S)-verbenone, (1R,5R)-verbenone, oxalacetic acid, and (2S,4S)-carveol do not serve as substrate
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-
?
additional information
?
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-
(R)-carvone, (S)-carvone, (1S,5S)-verbenone, (1R,5R)-verbenone, oxalacetic acid, and (2S,4S)-carveol do not serve as substrate
-
-
?
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allyl alcohol + NADP+
acrolein + NADPH
-
-
-
-
r
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NADP+
-
activity 4times greater than with NAD+
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8.3
(S)-pulegone
recombinant enzyme, pH 7.0, 35°C
0.025
(R)-pulegone
native enzyme, pH 7.0, 35°C
1.4
(R)-pulegone
recombinant enzyme, pH 7.0, 35°C
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3.3
(R)-pulegone
recombinant enzyme, pH 7.0, 35°C
2.8
(S)-pulegone
recombinant enzyme, pH 7.0, 35°C
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7.4
-
reduction of carbonyl group of (S)-carvone
8
-
dehydrogenation of (S)-carveol
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UniProt
brenda
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DBR_TOBAC
343
0
38088
Swiss-Prot
-
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36300
2 * 36300, SDS-PAGE, native mass by gel filtration
38482
2 * 38482, recombinant protein, MALDI TOF-MS, native mass by gel filtration
38487
2 * 38487, recombinant protein, calculated from the deduced amino acid sequence, native mass by gel filtration
37000
-
2 * 37000, SDS-PAGE and amino acid sequence analysis
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dimer
-
2 * 37000, SDS-PAGE and amino acid sequence analysis
dimer
2 * 36300, SDS-PAGE, native mass by gel filtration
dimer
2 * 38482, recombinant protein, MALDI TOF-MS, native mass by gel filtration
dimer
2 * 38487, recombinant protein, calculated from the deduced amino acid sequence, native mass by gel filtration
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native enzyme, recombinant enzyme purified using GST-tag
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GST-fusion protein expressed in Escherichia coli BL21
sequence homology with other NADPH-dependent oxidoreductases from plants
-
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Hirata, T.; Tamura, Y.; Yokobatake, N.; Shimoda, K.; Ashida, Y.
A 38 kDa allylic alcohol dehydrogenase from the cultured cells of Nicotiana tabacum
Phytochemistry
55
297-303
2000
Nicotiana tabacum
brenda
Matsushima, A.; Sato, Y.; Otsuka, M.; Watanabe, T.; Yamamoto, H.; Hirata, T.
An enone reductase from Nicotiana tabacum: cDNA cloning, expression in Escherichia coli, and reduction of enones with the recombinant proteins
Bioorg. Chem.
36
23-28
2008
Nicotiana tabacum (Q9SLN8), Nicotiana tabacum
brenda