We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments The enzyme catalyses a step of the pentose phosphate pathway. The enzyme is specific for NADP+. cf. EC 1.1.1.363, glucose-6-phosphate dehydrogenase [NAD(P)+] and EC 1.1.1.388, glucose-6-phosphate dehydrogenase (NAD+).
The taxonomic range for the selected organisms is: Arabidopsis thaliana The enzyme appears in selected viruses and cellular organisms
Synonyms
g6pd, glucose-6-phosphate dehydrogenase, g6pdh, g-6-pd, glucose 6-phosphate dehydrogenase, g-6-pdh, g-6pd, zwf-1, glucose-6-phosphate 1-dehydrogenase, glc6pdh,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6-phosphoglucose dehydrogenase
-
-
-
-
D-glucose 6-phosphate dehydrogenase
-
-
-
-
Entner-Doudoroff enzyme
-
-
-
-
glucose 6-phosphate dehydrogenase (NADP)
-
-
-
-
glucose-6-phosphate 1-dehydrogenase
-
-
-
-
glucose-6-phosphate dehydrogenase
-
-
-
-
NADP-dependent glucose 6-phophate dehydrogenase
-
-
-
-
NADP-glucose-6-phosphate dehydrogenase
-
-
-
-
Vegetative protein 11
-
-
-
-
G6PD1
-
-
-
-
G6PD2
-
-
-
-
G6PD4
-
-
-
-
G6PD5
-
-
-
-
G6PD6
-
-
-
-
G6PDH
-
-
G6PDH1
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D-glucose-6-phosphate:NADP+ 1-oxidoreductase
The enzyme catalyses a step of the pentose phosphate pathway. The enzyme is specific for NADP+. cf. EC 1.1.1.363, glucose-6-phosphate dehydrogenase [NAD(P)+] and EC 1.1.1.388, glucose-6-phosphate dehydrogenase (NAD+).
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
additional information
?
-
-
G6PDH plays a role in supplying NADPH for oil accumulation in developing seeds in which photosynthesis may be light limited
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
-
during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
during 7 days of phosphate starvation, G6PD5 is continuously expressed throughout phosphate-starvation
-
additional information
during 7 days of phosphate starvation, G6PD5 is continuously expressed throughout phosphate-starvation
-
additional information
during 7 days of phosphate starvation, G6PD5 is continuously expressed throughout phosphate-starvation
-
additional information
during 7 days of phosphate starvation, G6PD5 is continuously expressed throughout phosphate-starvation
-
additional information
during 7 days of phosphate starvation, G6PD5 is continuously expressed throughout phosphate-starvation
-
additional information
during 7 days of phosphate starvation, G6PD5 is continuously expressed throughout phosphate-starvation
-
additional information
-
during 7 days of phosphate starvation, G6PD5 is continuously expressed throughout phosphate-starvation
-
additional information
during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
-
during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
Trx f1 regulates G6PDH1 activity as efficiently as Trx m1 or m4. Trx x is a very poor regulator of G6PDH activity. Trx y1 is inefficient as inhibitor but it shows high efficiency in activation. Upon illumination, a strong and fast reductive inhibition of G6PDH1 activity dependent on the presence of all the components of the Fd/Trx system
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
anhydrotetracyclin
induces expression of mature G6PDH1
sucrose
-
in wild-type seedlings G6PD5 and G6PD6 show small (1.7- and 2fold, respectively) but significant induction by sucrose
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
-
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
-
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
oxidative activation is strictly dependent on plastidial thioredoxins that show differential efficiencies. Trx f1 regulates G6PDH1 activity as efficiently as Trx m1 or m4. Trx x is a very poor regulator of G6PDH activity. Trx y1 is inefficient as inhibitor but it shows high efficiency in activation. G6PDH activity can be recovered by transferring from light to dark, reversibility of inactivation certifying redox modulation of the enzyme. In the dark, reactivation can be enhanced by the addition of oxidants, such as oxidized dithiothreitol and H2O2
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.57
D-glucose 6-phosphate
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
11.23
purified G6PDH1 upon reduction by reduced DTT
139.5
purified G6PDH1 upon oxidation by diamide
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
-
brenda
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
brenda
-
brenda
-
brenda
-
brenda
-
G6PD5 and G6PD6
brenda
-
-
brenda
-
brenda
additional information
the levels of transcripts are low at day 0 and increase markedly at day 1, then gradually decrease
-
brenda
additional information
the levels of transcripts are low at day 0 and increase markedly at day 1, then gradually decrease
-
brenda
additional information
the levels of transcripts are low at day 0 and increase markedly at day 1, then gradually decrease
-
brenda
additional information
the levels of transcripts are low at day 0 and increase markedly at day 1, then gradually decrease
-
brenda
additional information
the levels of transcripts are low at day 0 and increase markedly at day 1, then gradually decrease
-
brenda
additional information
the levels of transcripts are low at day 0 and increase markedly at day 1, then gradually decrease
-
brenda
additional information
-
the levels of transcripts are low at day 0 and increase markedly at day 1, then gradually decrease
-
brenda
additional information
the levels of transcripts are low at day 0 and increase markedly at day 1, then gradually decrease
-
brenda
additional information
the levels of transcripts are low at day 0 and increase markedly at day 1, then gradually decrease
-
brenda
additional information
the levels of transcripts are low at day 0 and increase markedly at day 1, then gradually decrease
-
brenda
additional information
the levels of transcripts are low at day 0 and increase markedly at day 1, then gradually decrease
-
brenda
additional information
the levels of transcripts are low at day 0 and increase markedly at day 1, then gradually decrease
-
brenda
additional information
the levels of transcripts are low at day 0 and increase markedly at day 1, then gradually decrease
-
brenda
additional information
-
the levels of transcripts are low at day 0 and increase markedly at day 1, then gradually decrease
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
G6PD3_ARATH
599
0
67358
Swiss-Prot
Chloroplast (Reliability: 2 )
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
activities of the cytosolic isoforms G6PD5 and G6PD6 are reciprocally increased in single mutants with no increase of their respective transcript levels. Seeds of the double mutant but not of the single mutants have higher oil content and increased weight compared to those of the wild-type, with no alteration in the carbon to nitrogen ratio or fatty acid composition. Total G6PDH activity is reduced only in the double mutant
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-20°C, 100 mM Tris-HCl buffer, pH 7.9, several months
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
by affinity chromatography, to homogeneity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
into vector pET16b and expressed in a G6PDH-deficient Escherichia coli strain. Overexpression in the cytosol of the susceptible tobacco cultivar Xanthi
expressed in Escherichia coli DH5alpha strain from the pASK-G6PDH1 plasmid
transient expression of the G6PD5 and G6PD6 fused to a GFP gene, cloned into the SpeI site of pCAMBIA1302. T-DNA insertion lines transformed with cosmid clones containing the regions of G6PD5 and G6PD6 isolated from a genomic cosmid (pBIC20) library
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
because isoenzyme replacement of G6PDH in the cytosol of tobacco is beneficial under various kinds of cues, this strategy may be a tool to enhance stress tolerance in general
additional information
-
loss of cytosolic G6PDH activity affects the metabolism of developing seeds by increasing carbon substrates for synthesis of storage compounds rather than by decreasing the NADPH supply specifically for fatty acid synthesis
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Nee, G.; Zaffagnini, M.; Trost, P.; Issakidis-Bourguet, E.
Redox regulation of chloroplastic glucose-6-phosphate dehydrogenase: a new role for f-type thioredoxin
FEBS Lett.
583
2827-2832
2009
Arabidopsis thaliana (Q43727)
brenda
Wakao, S.; Andre, C.; Benning, C.
Functional analyses of cytosolic glucose-6-phosphate dehydrogenases and their contribution to seed oil accumulation in arabidopsis
Plant Physiol.
146
277-288
2008
Arabidopsis thaliana
brenda
Scharte, J.; Schoen, H.; Tjaden, Z.; Weis, E.; von Schaewen, A.
Isoenzyme replacement of glucose-6-phosphate dehydrogenase in the cytosol improves stress tolerance in plants
Proc. Natl. Acad. Sci. USA
106
8061-8066
2009
Nicotiana tabacum, Arabidopsis thaliana (Q8L743)
brenda
Yin, Y.; Ashihara, H.
Expression of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase isoform genes in suspension-cultured Arabidopsis thaliana cells
Z. Naturforsch. C
63
713-720
2008
Arabidopsis thaliana (Q43727), Arabidopsis thaliana (Q8L743), Arabidopsis thaliana (Q93ZW0), Arabidopsis thaliana (Q9FJI5), Arabidopsis thaliana (Q9FY99), Arabidopsis thaliana (Q9LK23), Arabidopsis thaliana, Arabidopsis thaliana T87 (Q43727), Arabidopsis thaliana T87 (Q8L743), Arabidopsis thaliana T87 (Q93ZW0), Arabidopsis thaliana T87 (Q9FJI5), Arabidopsis thaliana T87 (Q9FY99), Arabidopsis thaliana T87 (Q9LK23)
brenda