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Information on EC 1.1.1.42 - isocitrate dehydrogenase (NADP+) and Organism(s) Arabidopsis thaliana and UniProt Accession Q9SRZ6
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1.1.1.42 isocitrate dehydrogenase (NADP+)IUBMB Comments
Requires Mn2+ or Mg2+ for activity. Unlike EC 1.1.1.41, isocitrate dehydrogenase (NAD+), oxalosuccinate can be used as a substrate. In eukaryotes, isocitrate dehydrogenase exists in two forms: an NAD+-linked enzyme found only in mitochondria and displaying allosteric properties, and a non-allosteric, NADP+-linked enzyme that is found in both mitochondria and cytoplasm . The enzyme from some species can also use NAD+ but much more slowly [6,7].
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- 1.1.1.42
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progression-free
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neomorphic
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nadph-generating
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o6-methylguanine-dna
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gliomagenesis
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oligodendroglial
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pilocytic
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karnofsky
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proneural
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
isocitrate dehydrogenase 1, nadp-isocitrate dehydrogenase, nadp-dependent isocitrate dehydrogenase, isocitrate dehydrogenase-1, nadp-icdh, nadp-idh, nadp+-dependent isocitrate dehydrogenase, nadp-linked isocitrate dehydrogenase, nadp-specific isocitrate dehydrogenase, nadp+-specific isocitrate dehydrogenase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
CtIDP1
-
-
-
-
CtIDP2
-
-
-
-
IDH
-
-
-
-
IDP
-
-
-
-
isocitrate dehydrogenase (NADP)
-
-
-
-
isocitrate dehydrogenase (NADP-dependent)
-
-
-
-
isocitrate dehydrogenase (nicotinamide adenine dinucleotide phosphate)
-
-
-
-
NADP isocitric dehydrogenase
-
-
-
-
NADP+-linked isocitrate dehydrogenase
-
-
-
-
NADP+-specific ICDH
-
-
-
-
NADP-dependent isocitrate dehydrogenase
-
-
-
-
NADP-dependent isocitric dehydrogenase
-
-
-
-
NADP-linked isocitrate dehydrogenase
-
-
-
-
NADP-specific isocitrate dehydrogenase
-
-
-
-
oxalosuccinate decarboxylase
-
-
-
-
oxalsuccinic decarboxylase
-
-
-
-
PS-NADP-IDH
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
redox reaction
-
-
-
-
oxidative decarboxylation
-
-
-
-
reductive carboxylation
-
-
-
-
PATHWAY SOURCE
PATHWAYS
KEGG
-
-, -, -, -, -, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
isocitrate:NADP+ oxidoreductase (decarboxylating)
Requires Mn2+ or Mg2+ for activity. Unlike EC 1.1.1.41, isocitrate dehydrogenase (NAD+), oxalosuccinate can be used as a substrate. In eukaryotes, isocitrate dehydrogenase exists in two forms: an NAD+-linked enzyme found only in mitochondria and displaying allosteric properties, and a non-allosteric, NADP+-linked enzyme that is found in both mitochondria and cytoplasm [6]. The enzyme from some species can also use NAD+ but much more slowly [6,7].
CAS REGISTRY NUMBER
COMMENTARY
9028-48-2
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3-morpholinosydnonimine
44% inhibition at 5 mM of leaf and root enzymes
GSH
inhibits the leaf enzyme by 40% at 5 mM, but not the root enzyme
GSSG
increasing GSSG concentrations progressively inhibit the enzyme activity, almost complete inhibition at 1 mM
H2O2
affects the root enzyme slightly but not the enzyme from leaves
nitrosoglutathione
increasing nitrosoglutathione concentrations progressively inhibit the enzyme activity, complete inhibition at 2 mM
S-nitrosoglutathione
70.5% inhibition at 5 mM of the leaf enzyme, 37.3% of the root enzyme
additional information
increasing concentrations of H2O2 hardly affect the enzyme activity
-
additional information
-
increasing concentrations of H2O2 hardly affect the enzyme activity
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
Arabidopsis thaliana has three NADP-ICDH genes At1g65930, At5g14590, and At1g54340, coding for the proteins NP_176768, NP_196963, and BT025983, respectively, that are located in different subcellular compartments, i.e. cytosol, chloroplasts, mitochondria, and peroxisomes
physiological function
-
cICDH is involved in amino acid synthesis, and plays a role in redox signalling linked to pathogen responses, but cICDH is not required for plant development and primary metabolism in optimal growth conditions
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). ICDHC_ARATH
410
0
45746
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
homodimer
2 * 48300, His-tagged enzyme, calculated from amino acid sequence
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glutathionylation
S-glutathionylation of Cys363 is involved in the inhibition of the cytosolic enzyme activity upon nitrosoglutathione treatments
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
C363S
the mutation does not perturb the enzymatic characteristics of the enzyme. However, the mutant is less affected by nitrosoglutathione treatment than the wild type enzyme
additional information
additional information
-
introduction of an icdh mutation into the cat2 background, in which increased availability of H2O2 causes perturbed redox homeostasis and induction of stress-related genes. Accumulation of oxidized glutathione and pathogen-related responses are enhanced in double cat2 icdh mutants compared to cat2. Single icdh mutants present constitutive induction of PR genes, enhanced resistance to bacteria in icdh, cat2 and cat2 icdh is quantitatively correlated with PR gene expression. However, the effect of icdh in both Col0 and cat2 backgrounds is not associated with enhanced accumulation of salicylic acid, phenotypes, overview
additional information
identification of naturally occurring peroxisomal NADP-ICDH (picdh) Arabidopsis mutant lines, designated as picdh-1 (SALK_072422) and picdh-2 (SALK_039193C) carrying a T-DNA insertion in exons 9 and in the 5'UTR, respectively. The mutation results in a lack of the peroxisomal NADP-ICDH in both picdh-1 and picdh-2 plants. There are no significant differences in phenotype between the wild-type (Wt) and the two mutant (picdh-1 and picdh-2) lines. Intrinsic photosynthetic performance is not altered in the different plants with similar maximum C assimilation capacities, light use efficiency, and respiration rates in darkness due to similar growth rates. No differences are found in growth rates, stomatal densities, or leaf mass-area ratios
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme activity is affected by oxidative agents like oxidized glutathione and nitrosoglutathione through modifications of conserved Cys residues in the protein
760361
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene pICDH, DNA and amino acid sequence determination and analysis, phylogenetic analysis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mhamdi, A.; Mauve, C.; Gouia, H.; Saindrenan, P.; Hodges, M.; Noctor, G.
Cytosolic NADP-dependent isocitrate dehydrogenase contributes to redox homeostasis and the regulation of pathogen responses in Arabidopsis leaves
Plant Cell Environ.
33
1112-1123
2010
Arabidopsis thaliana, Arabidopsis thaliana Columbia ecotype
Leterrier, M.; Barroso, J.; Palma, J.; Corpas, F.
Cytosolic NADP-isocitrate dehydrogenase in Arabidopsis leaves and roots
Biol. Plant.
56
705-710
2012
Arabidopsis thaliana (Q9SRZ6)
-
Leterrier, M.; Barroso, J.B.; Valderrama, R.; Begara-Morales, J.C.; Sanchez-Calvo, B.; Chaki, M.; Luque, F.; Vinegla, B.; Palma, J.M.; Corpas, F.J.
Peroxisomal NADP-isocitrate dehydrogenase is required for Arabidopsis stomatal movement
Protoplasma
253
403-415
2016
Arabidopsis thaliana (Q9SLK0), Arabidopsis thaliana Col-0 (Q9SLK0)
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