Information on EC 1.1.1.398 - 2-glutathionyl-2-methylbut-3-en-1-ol dehydrogenase

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The expected taxonomic range for this enzyme is: Rhodococcus sp. AD45

EC NUMBER
COMMENTARY hide
1.1.1.398
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RECOMMENDED NAME
GeneOntology No.
2-glutathionyl-2-methylbut-3-en-1-ol dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-(glutathione-S-yl)-2-methylbut-3-en-1-ol + 2 NAD+ + H2O = 2-(glutathione-S-yl)-2-methylbut-3-enoate + 2 NADH + 2 H+
show the reaction diagram
overall reaction
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2-(glutathione-S-yl)-2-methylbut-3-en-1-ol + NAD+ = 2-(glutathione-S-yl)-2-methylbut-3-enal + NADH + H+
show the reaction diagram
(1a)
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2-(glutathione-S-yl)-2-methylbut-3-enal + NAD+ + H2O = 2-(glutathione-S-yl)-2-methylbut-3-enoate + NADH + H+
show the reaction diagram
(1b)
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SYSTEMATIC NAME
IUBMB Comments
2-(glutathione-S-yl)-2-methylbut-3-en-1-ol:NAD+ oxidoreductase
The enzyme, characterized from the bacterium Rhodococcus sp. AD45, is involved in isoprene degradation.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-hydroxy-2-glutathionyl-2-methyl-3-butene + NAD+ + H2O
1-oxo-2-glutathionyl-2-methyl-3-butene + NADH + H+
show the reaction diagram
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?
2-glutathionyl-2-methyl-3-butenal + NAD+ + H2O
2-glutathionyl-2-methyl-3-butenoic acid + NADH + H+
show the reaction diagram
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?
additional information
?
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no NADH formation is observed when GSH, methanol, ethanol, glycerol, 2-methylbutanol, 3-methyl-butanol, 1-hydroxy-3-methyl-3-butene, or 1,2-dihydroxy-2-methyl-3-butene are used as a substrate. The conjugates of GSH with 1,2-epoxybutane and 1,2-epoxyhexane are also not substrates
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.4
1-hydroxy-2-glutathionyl-2-methyl-3-butene
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at pH 10.0 and 30C
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0.07
NAD+
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at pH 10.0 and 30C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.9
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crude extract, at pH 10.0 and 30C
18
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after 6.2fold purification, at pH 10.0 and 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
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2 * 25000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Blue Sepharose column chromatography, DE52 column chromatography, and Sephacryl S-300 gel filtration
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