Information on EC 1.1.1.392 - 3alpha-hydroxycholanate dehydrogenase (NADP+)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eggerthella lenta

EC NUMBER
COMMENTARY hide
1.1.1.392
-
RECOMMENDED NAME
GeneOntology No.
3alpha-hydroxycholanate dehydrogenase (NADP+)
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
lithocholate + NADP+ = 3-oxo-5beta-cholan-24-oate + NADPH + H+
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
iso-bile acids biosynthesis II
-
-
Secondary bile acid biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
lithocholate:NADP+ 3-oxidoreductase
This bacterial enzyme is involved in the modification of secondary bile acids to form 3beta-bile acids (also known as iso-bile acids) via a 3-oxo intermediate. The enzyme catalyses a reversible reaction in vitro. Also acts on related bile acids. cf. EC 1.1.1.52, 3alpha-hydroxycholanate dehydrogenase (NAD+).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene Elen_0690
UniProt
Manually annotated by BRENDA team
gene Elen_0690
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1280
12alpha-Hydroxy-3-oxo-5beta-cholan-24-oic acid
pH 7.0, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.85
12alpha-Hydroxy-3-oxo-5beta-cholan-24-oic acid
Eggerthella lenta
C8WMP0
pH 7.0, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene Elen_0690, DNA and amino acid sequence determination and analysis, recombinant overvexpression in Escherichia coli