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Information on EC 1.1.1.37 - malate dehydrogenase and Organism(s) Mesembryanthemum crystallinum and UniProt Accession O24047

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EC Tree
     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.37 malate dehydrogenase
IUBMB Comments
There are several forms of malate dehydrogenases that differ by their use of substrate and cofactors. This NAD+-dependent enzyme forms oxaloacetate and unlike EC 1.1.1.38, malate dehydrogenase (oxaloacetate-decarboxylating), is unable to convert it to pyruvate. Also oxidizes some other 2-hydroxydicarboxylic acids. cf. EC 1.1.1.82, malate dehydrogenase (NADP+); EC 1.1.1.299, malate dehydrogenase [NAD(P)+]; and EC 1.1.5.4, malate dehydrogenase (quinone).
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Mesembryanthemum crystallinum
UNIPROT: O24047
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The taxonomic range for the selected organisms is: Mesembryanthemum crystallinum
The enzyme appears in selected viruses and cellular organisms
Synonyms
malate dehydrogenase, mdh, mitochondrial malate dehydrogenase, malic dehydrogenase, cytosolic malate dehydrogenase, nad-dependent malate dehydrogenase, maldh, mitochondrial mdh, s-mdh, l-malate dehydrogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(R)-2-hydroxyacid dehydrogenase
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-
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L-malate dehydrogenase
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malate (NAD) dehydrogenase
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malate dehydrogenase (NAD)
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malic acid dehydrogenase
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malic dehydrogenase
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mbNAD-MDH
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-
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mNAD-MDH
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-
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NAD-dependent malate dehydrogenase
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-
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NAD-dependent malic dehydrogenase
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-
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NAD-L-malate dehydrogenase
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NAD-linked malate dehydrogenase
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NAD-malate dehydrogenase
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NAD-malic dehydrogenase
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NAD-specific malate dehydrogenase
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VEG69
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Vegetative protein 69
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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reduction
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-
SYSTEMATIC NAME
IUBMB Comments
(S)-malate:NAD+ oxidoreductase
There are several forms of malate dehydrogenases that differ by their use of substrate and cofactors. This NAD+-dependent enzyme forms oxaloacetate and unlike EC 1.1.1.38, malate dehydrogenase (oxaloacetate-decarboxylating), is unable to convert it to pyruvate. Also oxidizes some other 2-hydroxydicarboxylic acids. cf. EC 1.1.1.82, malate dehydrogenase (NADP+); EC 1.1.1.299, malate dehydrogenase [NAD(P)+]; and EC 1.1.5.4, malate dehydrogenase (quinone).
CAS REGISTRY NUMBER
COMMENTARY hide
9001-64-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-oxoglutarate + NADH + H+
2-hydroxyglutarate + NAD+
show the reaction diagram
about 10% of the activity with oxaloacetate at pH 6.8 and at pH 8.2
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-
?
3-bromopyruvate + NADH
3-bromo-2-hydroxypropanoate + NAD+
show the reaction diagram
about 10% of the activity with oxaloacetate at pH 6.8
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-
?
alpha-ketoisovalerate + NADH
2-hydroxyisovalerate + NAD+
show the reaction diagram
about 10% of the activity with oxaloacetate at pH 8.2
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-
?
oxaloacetate + NADH
L-malate + NAD+
show the reaction diagram
the ratio of oxaloacetate reduction to malate oxidation is 28.4
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-
r
phenylpyruvate + NADH
2-hydroxy-3-phenylpropanoate + NAD+
show the reaction diagram
about 30% of the activity with oxaloacetate at pH 8.2
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-
?
pyruvate + NADH
2-hydroxypropanoate + NAD+
show the reaction diagram
about 25% of the activity with oxaloacetate at pH 8.2
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-
?
(S)-malate + NAD+
oxaloacetate + NADH + H+
show the reaction diagram
-
-
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r
malate + NAD+
oxaloacetate + NADH + H+
show the reaction diagram
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-
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r
oxaloacetate + NADH
L-malate + NAD+
show the reaction diagram
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-
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r
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
malate + NAD+
oxaloacetate + NADH + H+
show the reaction diagram
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r
additional information
?
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the enzyme plays a central role in Crassulacean acid metabolism
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0341
L-malate
pH 8.2, 30°C
0.319
NAD+
pH 8.2, 30°C
0.0136 - 0.0366
NADH
0.0088 - 0.0449
oxaloacetate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.225 - 0.775
oxaloacetate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 9.5
reduction of oxaloacetate
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
reduction of oxaloacetate
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 58
30°C: about 50% of maximal activity, 58°C: about 55% of maximal activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
MDHC_MESCR
332
0
35498
Swiss-Prot
other Location (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
39500
x * 39500, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
5 min, about 80% loss of activity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cuevas, I.C.; Podesta, F.E.
Purification and physical and kinetic characterization of an NAD+-dependent malate dehydrogenase from leaves of pineapple (Ananas comosus)
Physiol. Plant.
108
240-248
2000
Ananas comosus, Arabidopsis thaliana, Bryophyllum calycinum, Citrullus lanatus, Echinococcus granulosus, Mesembryanthemum crystallinum, Opuntia ficus-indica, Zea mays
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Manually annotated by BRENDA team
Tripodi, K.E.J.; Gomez Casati, M.E.; Uttaro, A.D.; Podesta, F.E.
Expression and characterization of recombinant cytosolic NAD+-dependent malate dehydrogenase from Mesembryanthemum crystallinum
Physiol. Plant.
117
222-228
2003
Mesembryanthemum crystallinum (O24047)
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Manually annotated by BRENDA team