Information on EC 1.1.1.368 - 6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase

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The expected taxonomic range for this enzyme is: Thauera aromatica

EC NUMBER
COMMENTARY hide
1.1.1.368
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RECOMMENDED NAME
GeneOntology No.
6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
6-hydroxycyclohex-1-ene-1-carbonyl-CoA + NAD+ = 6-oxocyclohex-1-ene-1-carbonyl-CoA + NADH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Benzoate degradation
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benzoyl-CoA degradation II (anaerobic)
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crotonate fermentation (to acetate and cyclohexane carboxylate)
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Metabolic pathways
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Microbial metabolism in diverse environments
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benzoyl-CoA degradation
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SYSTEMATIC NAME
IUBMB Comments
6-hydroxycyclohex-1-ene-1-carbonyl-CoA:NAD+ 6-oxidoreductase
The enzyme participates in the central benzoyl-CoA degradation pathway of some anaerobic bacteria such as Thauera aromatica.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
6-hydroxycyclohex-1-ene-1-carbonyl-CoA + NAD+
6-oxocyclohex-1-ene-1-carbonyl-CoA + NADH + H+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
6-hydroxycyclohex-1-ene-1-carbonyl-CoA + NAD+
6-oxocyclohex-1-ene-1-carbonyl-CoA + NADH + H+
show the reaction diagram
O87871
the enzyme participates in the central benzoyl-CoA degradation pathway of some anaerobic bacteria
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ir
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
the enzyme is specific for NAD+, no activity with NADP+
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.06
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
pH 7.4, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
Thauera aromatica
O87871
pH 7.4, 37°C, calculated as kcat number per subunit
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11.8
pH 7.4, 37°C, substrate: 6-hydroxycyclohex-1-ene-1-carbonyl-CoA
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
when cells are grown aerobically with benzoate the activity is 20-25fold lower than in cells grown anaerobically with benzoate
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38000
x * 38000, SDS-PAGE
78000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 38000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant protein
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
overexpressed in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression of the enzyme activity is under negative control by oxygen
gene expression is positively controlled by benzoate, an intermediate of the central benzoyl-CoA pathway