Information on EC 1.1.1.340 - 1-deoxy-11beta-hydroxypentalenate dehydrogenase

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The expected taxonomic range for this enzyme is: Streptomyces avermitilis

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EC NUMBER
COMMENTARY hide
1.1.1.340
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RECOMMENDED NAME
GeneOntology No.
1-deoxy-11beta-hydroxypentalenate dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1-deoxy-11beta-hydroxypentalenate + NAD+ = 1-deoxy-11-oxopentalenate + NADH + H+
show the reaction diagram
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
neopentalenoketolactone and pentalenate biosynthesis
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pentalenolactone biosynthesis
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
1-deoxy-11beta-hydroxypentalenate:NAD+ oxidoreductase
Isolated from the bacterium Streptomyces avermitilis and present in many other Streptomyces species. Part of the pathway for pentalenolactone biosynthesis.
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-deoxy-11beta-hydroxypentalenic acid + NAD+
1-deoxy-11-oxopentalenic acid + NADH + H+
show the reaction diagram
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-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
150fold preference for NAD+ over NADP+
NADP+
150fold preference for NAD+ over NADP+
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0065
1-deoxy-11beta-hydroxypentalenic acid
pH 8.0, 22°C
0.025
NAD+
pH 8.0, 22°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.65
1-deoxy-11beta-hydroxypentalenic acid
Streptomyces avermitilis
Q82IY9
pH 8.0, 22°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
and second maximum at pH 11.3
11.3
activity increases from pH 9.0 to pH 11.3, and is 12fold higher at pH 11.3 than at pH 8.0
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 30183, MALDI-TOF, recombinant protein including His-tag
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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LINKS TO OTHER DATABASES (specific for EC-Number 1.1.1.340)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)