Information on EC 1.1.1.328 - nicotine blue oxidoreductase

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The expected taxonomic range for this enzyme is: Paenarthrobacter nicotinovorans

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EC NUMBER
COMMENTARY hide
1.1.1.328
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RECOMMENDED NAME
GeneOntology No.
nicotine blue oxidoreductase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3,3'-bipyridine-2,2',5,5',6,6'-hexol + NAD(P)+ = (E)-2,2',5,5'-tetrahydroxy-6H,6'H-[3,3'-bipyridinylidene]-6,6'-dione + NAD(P)H + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
nicotine degradation I (pyridine pathway)
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Nicotinate and nicotinamide metabolism
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
3,3'-bipyridine-2,2',5,5',6,6'-hexol:NADP+ 11-oxidoreductase
The enzyme, characterized from the nicotine degrading bacterium Arthrobacter nicotinovorans, catalyses the reduction of "nicotine blue" to its hydroquinone form (the opposite direction from that shown). Nicotine blue is the name given to the compound formed by the autocatalytic condensation of two molecules of 2,3,6-trihydroxypyridine, an intermediate in the nicotine degradation pathway. The main role of the enzyme may be to prevent the intracellular formation of nicotine blue semiquinone radicals, which by redox cycling would lead to the formation of toxic reactive oxygen species. The enzyme possesses a slight preference for NADH over NADPH.
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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enzyme is part of the nicotine catabolic pathway
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,4-benzoquinone + NADPH + H+
1,4-benzoquinol + NADP+
show the reaction diagram
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?
1,4-naphthoquinone + NADPH + H+
1,4-naphthoquinol + NADP+
show the reaction diagram
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?
4,4',5,5'-tetrahydroxy-3,3'-diazadiphenoquinone-(2,2') + NADPH + H+
? + NADP+
show the reaction diagram
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?
duroquinone + NADPH + H+
duroquinol + NADP+
show the reaction diagram
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?
menadione + NADPH + H+
menadiol + NADP+
show the reaction diagram
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?
nicotine blue + NADH + H+
nicotine blue leuco form + NAD+
show the reaction diagram
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two-electron transfer reaction
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?
nicotine blue + NADPH + H+
nicotine blue leuco form + NADP+
show the reaction diagram
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two-electron transfer reaction
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?
oxidized 2,6-dichlorophenol-indophenol + NADPH + H+
reduced 2,6-dichlorophenol-indophenol + NADP+
show the reaction diagram
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?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
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NADH is prefered over NADPH
NADPH
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70% of the rate with NADH
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0088
1,4-benzoquinone
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pH 7.1, temperature not specified in the publication
0.0062
1,4-Naphthoquinone
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pH 7.1, temperature not specified in the publication
0.023
2,6-dichlorophenol-indophenol
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pH 7.1, temperature not specified in the publication
0.035
NADH
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pH 7.1, temperature not specified in the publication
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.004
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substrate duraquinone, pH 7.1, temperature not specified in the publication
0.102
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substrate menadione, pH 7.1, temperature not specified in the publication
0.368
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substrate 2,6-dichlorophenol-indophenol, pH 7.1, temperature not specified in the publication
0.536
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substrate 1,4-benzoquinone, pH 7.1, temperature not specified in the publication
0.733
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substrate 1,4-naphthoquinone, pH 7.1, temperature not specified in the publication
0.893
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substrate nicotine blue, pH 7.1, temperature not specified in the publication
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme gene is expressed in a nicotine-dependent manner under the control of the transcriptional activator PmfR
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LINKS TO OTHER DATABASES (specific for EC-Number 1.1.1.328)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)