Information on EC 1.1.1.327 - 5-exo-hydroxycamphor dehydrogenase

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The expected taxonomic range for this enzyme is: Pseudomonas putida

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EC NUMBER
COMMENTARY hide
1.1.1.327
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RECOMMENDED NAME
GeneOntology No.
5-exo-hydroxycamphor dehydrogenase
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REACTION
REACTION DIAGRAM
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ORGANISM
UNIPROT
LITERATURE
5-exo-hydroxycamphor + NAD+ = bornane-2,5-dione + NADH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
(+)-camphor degradation
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SYSTEMATIC NAME
IUBMB Comments
5-exo-hydroxycamphor:NAD+ oxidoreductase
Contains Zn2+. Isolated from Pseudomonas putida, and involved in degradation of (+)-camphor.
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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Pseudomonas putida PpGl, which carries the CAM plasmid encoding enzymes involved in the degradation pathway of D-camphor, can utilize D-camphor as a sole carbon source. The enzymes responsible for early steps in D-camphor degradation are 5-exo-hydroxycamphor dehydrogenase (camD gene), cytochrome P-450cam (camC), NADH-putidaredoxin reductase (camA), and putidaredoxin (camB)
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
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two firmly bound zinc atoms per subunit. All three ligands, C40, H62 and C158 to the catalytic zinc atom in the active-site, and all four ligands, C98, C101, C104 and Cll2 to the second zinc atom, are conserved
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
gene camD encoding 5-exo-hydroxycamphor dehydrogenase is plasmid born, cotransferable by conjugation, mitomycin curable, and shows a higher segregation rate from cells that are multiplasmid rather than carrying a single plasmid
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the genes of the CAM plasmid encoding enzymes involved in the degradation pathway of D-camphor form an operon, camDCAB, and are under negative control by the gene camR located immediately upstream from the camD gene
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LINKS TO OTHER DATABASES (specific for EC-Number 1.1.1.327)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)