the presence of 10 mM EDTA (10 mM), 1 mM pyrazole or 1 mM 1,10-phenanthroline in enzyme assays does not affect the activity, indicating that the enzyme does not contain easily accessible bivalent metal ions. (S)-1-phenylethanol oxidation is not inhibited by 0.1 mM 5,5'-dithiobis-(2-nitrobenzoic acid), 1 mM 4-hydroxymercuribenzoic acid or 1 mM N-ethylmaleimide, indicating that no accessible cysteine residues are required for catalysis
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sitting drop method in a buffer containing 12-18% PEG 8000, 0.05 M potassium phosphate, pH 7.5 and 3% 2-methyl-2,4-pentanediol. The structure of the apo-form of the enzyme is solved with a resolution at 2.1 A. The enzyme soaked with NAD+ and the inhibitor (R)-1-phenylethanol forms a different crystal form containing two subunits in the asymmetric unit