Information on EC 1.1.1.310 - (S)-sulfolactate dehydrogenase

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The expected taxonomic range for this enzyme is: Chromohalobacter salexigens

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EC NUMBER
COMMENTARY hide
1.1.1.310
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RECOMMENDED NAME
GeneOntology No.
(S)-sulfolactate dehydrogenase
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REACTION
REACTION DIAGRAM
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ORGANISM
UNIPROT
LITERATURE
(2S)-3-sulfolactate + NAD+ = 3-sulfopyruvate + NADH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
sulfolactate degradation I
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Cysteine and methionine metabolism
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SYSTEMATIC NAME
IUBMB Comments
(2S)-sulfolactate:NAD+ oxidoreductase
This enzyme, isolated from the bacterium Chromohalobacter salexigens DSM 3043, acts only on the (S)-enantiomer of 3-sulfolactate. Combined with EC 1.1.1.338, (2R)-3-sulfolactate dehydrogenase (NADP+), it provides a racemase system that converts (2S)-3-sulfolactate to (2R)-3-sulfolactate, which is degraded further by EC 4.4.1.24, (2R)-sulfolactate sulfo-lyase. The enzyme is specific for NAD+.
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
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LITERATURE
(Substrate)
COMMENTARY
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LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2S)-3-sulfolactate + NAD+
3-sulfopyruvate + NADH + H+
show the reaction diagram
3-sulfopyruvate + NADH + H+
(2S)-3-sulfolactate + NAD+
show the reaction diagram
additional information
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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SlcC is not inhibited by pyruvate or oxaloacetate (5 mM), (S)-3-phosphoglycerate, (R)-malate, (R)-lactate, (S)-malate or (S)-lactate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7
(2S)-3-sulfolactate
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apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C
0.14
3-sulfopyruvate
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apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C
2
NAD+
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apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C
0.05
NADH
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apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.2
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crude extracts of acetate-grown cells, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C
7.4
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crude extracts of sulfolactate-grown cells, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C
675
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enzyme from sulfolactate-grown cells after 91fold purification, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
61000
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native enzyme, gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
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at 4°C, SlcC remains stable for several weeks and can be frozen and thawed without loss of activity
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
at 4°C, SlcC remains stable for several weeks and can be frozen and thawed without loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Mono Q column chromatography, phenyl Superose gel filtration, Superose 12 gel filtration
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LINKS TO OTHER DATABASES (specific for EC-Number 1.1.1.310)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)