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Information on EC 1.1.1.303 - diacetyl reductase [(R)-acetoin forming] and Organism(s) Saccharomyces cerevisiae and UniProt Accession P39714

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IUBMB Comments
The reaction is catalysed in the reverse direction. This activity is usually associated with butanediol dehydrogenase activity (EC 1.1.1.4 or EC 1.1.1.76). While the butanediol dehydrogenase activity is reversible, diacetyl reductase activity is irreversible. This enzyme has been reported in the yeast Saccharomyces cerevisiae [1,2]. Different from EC 1.1.1.304, diacetyl reductase [(S)-acetoin forming].
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Saccharomyces cerevisiae
UNIPROT: P39714
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The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Reaction Schemes
Synonyms
(R,R)-BDH, (R,R)-butane-2,3-diol dehydrogenase, (R,R)-butanediol dehydrogenase/diacetyl reductase, acetoin/diacetyl reductase, BdhA, DAR, dudA, EC 1.1.1.5, gutB, More, more
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
(R)-acetoin:NAD+ oxidoreductase
The reaction is catalysed in the reverse direction. This activity is usually associated with butanediol dehydrogenase activity (EC 1.1.1.4 or EC 1.1.1.76). While the butanediol dehydrogenase activity is reversible, diacetyl reductase activity is irreversible. This enzyme has been reported in the yeast Saccharomyces cerevisiae [1,2]. Different from EC 1.1.1.304, diacetyl reductase [(S)-acetoin forming].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
diacetyl + NADH + H+
(R)-acetoin + NAD+
show the reaction diagram
51.4% of the (R)-2,3-butanediol dehydrogenase activity with substrate acetoin
-
-
ir
1,2-cyclohexanedione + NADH + H+
(R)-2-hydroxy-1-cyclohexanone + NAD+
show the reaction diagram
-
5% of the (R)-2,3-butanediol dehydrogenase activity with substrate acetoin
-
-
ir
2,3-pentanedione + NADH + H+
(3R)-3-hydroxy-2-pentanone + NAD+
show the reaction diagram
-
7% of the (R)-2,3-butanediol dehydrogenase activity with substrate acetoin
-
-
ir
diacetyl + NADH + H+
(R)-acetoin + NAD+
show the reaction diagram
-
21% of the (R)-2,3-butanediol dehydrogenase activity with substrate acetoin
-
-
ir
additional information
?
-
-
enzyme is specific for NADH
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
-
enzyme is specific for NADH
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dipicolinate
-
inhibition, (R)-2,3-butanediol dehydrogenase activity
EDTA
-
inhibition, (R)-2,3-butanediol dehydrogenase activity
o-phenanthroline
-
inhibition, (R)-2,3-butanediol dehydrogenase activity
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Mg2+
-
activation, (R)-2,3-butanediol dehydrogenase activity
Mn2+
-
activation, (R)-2,3-butanediol dehydrogenase activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
bifunctional diacetyl reductase and (R)-2,3-butanediol dehydrogenase
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
gene disruption is not lethal. Disrupted strains can grow on 2,3-butanediol, but to a lesser cell density than wild-type
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
140000
-
gel filtration
35000
-
4 * 35000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
-
4 * 35000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Heidlas, J.; Tressl, R.
Purification and characterization of a (R)-2,3-butanediol dehydrogenase from Saccharomyces cerevisiae
Arch. Microbiol.
154
267-273
1990
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Gonzalez, E.; Fernandez, M.R.; Larroy, C.; Sola, L.; Pericas, M.A.; Pares, X.; Biosca, J.A.
Characterization of a (2R,3R)-2,3-butaendiol dehydrogenase as the Saccharomyces cerevisiae YAL060W gene product
J. Biol. Chem.
275
35876-35885
2000
Saccharomyces cerevisiae (P39714)
Manually annotated by BRENDA team