Information on EC 1.1.1.291 - 2-hydroxymethylglutarate dehydrogenase

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The expected taxonomic range for this enzyme is: Eubacterium barkeri

EC NUMBER
COMMENTARY
1.1.1.291
-
RECOMMENDED NAME
GeneOntology No.
2-hydroxymethylglutarate dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(S)-2-hydroxymethylglutarate + NAD+ = 2-formylglutarate + NADH + H+
show the reaction diagram
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-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Nicotinate and nicotinamide metabolism
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nicotinate degradation III
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SYSTEMATIC NAME
IUBMB Comments
(S)-2-hydroxymethylglutarate:NAD+ oxidoreductase
NADP+ cannot replace NAD+. Forms part of the nicotinate-fermentation catabolism pathway in Eubacterium barkeri. Other enzymes involved in this pathway are EC 1.17.1.5 (nicotinate dehydrogenase), EC 1.3.7.1 (6-hydroxynicotinate reductase), EC 3.5.2.18 (enamidase), EC 5.4.99.4 (2-methyleneglutarate mutase), EC 5.3.3.6 (methylitaconate Delta-isomerase), EC 4.2.1.85 (dimethylmaleate hydratase) and EC 4.1.3.32 (2,3-dimethylmalate lyase).
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-formylglutarate + NADH + H+
2-hydroxymethylglutarate + NAD+
show the reaction diagram
-
the enzyme forms part of the nicotinate-fermentation catabolism pathway in Eubacterium barkeri
-
-
?
2-hydroxymethylglutarate + NAD+
2-formylglutarate + NADH + H+
show the reaction diagram
-
-
-
-
r
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-formylglutarate + NADH + H+
2-hydroxymethylglutarate + NAD+
show the reaction diagram
-
the enzyme forms part of the nicotinate-fermentation catabolism pathway in Eubacterium barkeri
-
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
NAD+
-
no activity with NADP+
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.1
-
2-hydroxymethylglutarate
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-
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
tetramer
-
4 * 32000, SDS-PAGE
OXIDATION STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
instability under anaerobic conditions
-
670692
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli
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