Information on EC 1.1.1.29 - glycerate dehydrogenase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
1.1.1.29
-
RECOMMENDED NAME
GeneOntology No.
glycerate dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
D-glycerate + NAD+ = hydroxypyruvate + NADH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
redox reaction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
-
-
Biosynthesis of secondary metabolites
-
-
Glycine, serine and threonine metabolism
-
-
Glyoxylate and dicarboxylate metabolism
-
-
Metabolic pathways
-
-
Methane metabolism
-
-
Microbial metabolism in diverse environments
-
-
photorespiration
-
-
serine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
D-glycerate:NAD+ oxidoreductase
-
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D-glycerate dehydrogenase
-
-
-
-
dehydrogenase, glycerate
-
-
-
-
GDH
-
-
-
-
glyoxylate reductase
-
-
-
-
HPR
-
-
-
-
hydroxypyruvate dehydrogenase
-
-
-
-
hydroxypyruvate reductase
-
-
-
-
hydroxypyruvate reductase
Q9C9W5
-
hydroxypyruvate reductase 1
-
-
NADH-dependent hydroxypyruvate reductase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9028-37-9
-
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
Q9C9W5
rates of photosynthesis under photorespiratory conditions are slightly reduced in mutant plants lacking isoform hpr1 expression or additionally lacking both of the peroxisomal malate dehydrogenase genes, mutant pmdh1pmdh2hpr1. The CO2 compensation points increase under photorespiratory conditions in both mutants. The increase in CO2 compensation points in the hpr1 and pmdh1pmdh2hpr1 plants is not associated with changes in mitochondrial respiration but with an increase in the non-respiratory CO2 released per Rubisco oxygenation reaction
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glyoxylate + NADH
glycolate + NAD+
show the reaction diagram
-
-
-
-
?
glyoxylate + NADH
glycolate + NAD+
show the reaction diagram
-
-
-
-
ir
hydroxypyruvate + NADH
D-glycerate + NAD+
show the reaction diagram
-
-
-
-
r
hydroxypyruvate + NADH
D-glycerate + NAD+
show the reaction diagram
-
-
-
-
r
hydroxypyruvate + NADH
D-glycerate + NAD+
show the reaction diagram
-
-
-
-
r
hydroxypyruvate + NADH
D-glycerate + NAD+
show the reaction diagram
-
-
-
-
-
hydroxypyruvate + NADH
D-glycerate + NAD+
show the reaction diagram
-
-
-
-
ir
hydroxypyruvate + NADH
D-glycerate + NAD+
show the reaction diagram
-
-
-
-
?
hydroxypyruvate + NADH
D-glycerate + NAD+
show the reaction diagram
-
-
-
-
?
hydroxypyruvate + NADH
D-glycerate + NAD+
show the reaction diagram
-
-
-
-
ir
hydroxypyruvate + NADH
D-glycerate + NAD+
show the reaction diagram
-
-
-
-
r
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
hydroxypyruvate + NADH
D-glycerate + NAD+
show the reaction diagram
-
-
-
-
r
hydroxypyruvate + NADH
D-glycerate + NAD+
show the reaction diagram
-
-
-
-
-
hydroxypyruvate + NADH
D-glycerate + NAD+
show the reaction diagram
-
-
-
-
ir
hydroxypyruvate + NADH
D-glycerate + NAD+
show the reaction diagram
-
-
-
-
?
hydroxypyruvate + NADH
D-glycerate + NAD+
show the reaction diagram
-
-
-
-
?
hydroxypyruvate + NADH
D-glycerate + NAD+
show the reaction diagram
-
-
-
-
ir
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Dihydroxyfumarate
-
-
NADH
-
concentration above 0.5 mM
NADPH
-
concentration above 0.5 mM
p-chloromercuribenzoate
-
-
phenylhydrazine
-
-
Hydroxypyruvate
-
product inhibition at a concentration above 4 mM, 99% inhibition of the reverse reaction at 0.5 mM
additional information
-
not inhibitory: citrate, NADP+, EDTA, 1,10-phenanthroline, iodoacetate, iodoacetamide, N-ethylmaleimide, hydroxylamine, semicarbazide, NaN3, NaF, KCN, L-ascorbate, dithiothreitol, pyridoxal 5'-phosphate
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.5
glyoxylate
-
-
10.8
glyoxylate
-
-
0.062
Hydroxypyruvate
-
-
0.09
Hydroxypyruvate
-
-
0.1
Hydroxypyruvate
-
-
0.12
Hydroxypyruvate
-
-
0.175
Hydroxypyruvate
-
-
0.0058
NADH
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
activities of several Hypomicrobium and Methylobacterium strains listed
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4 - 6.5
-
reduction of hydroxypyruvate
9 - 11
-
glycerate oxidation
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
69000 - 70000
-
gel filtration
286506
71000
-
SDS-PAGE
286508
90000
-
gel filtration
286509
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
-
2 * 38000, SDS-PAGE
dimer
-
2 * 37000, SDS-PAGE
dimer
-
2 * 45200, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 9
-
-
286506
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
-
up to
286506
30
-
30 min, 13.3% loss of activity
286506
35
-
30 min, 30.8% loss of activity
286506
40
-
30 min, 100% loss of activity
286506
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 10 mM potassium phosphate buffer, pH 7.0, 45% v/v glycerol, 1.0 M DTT, 18 months
-
4°C, 50 mM Tris-HCl buffer, pH 7.5, containing 10% glycerol and 1 mM dithiothreitol, 2 months, at least 90% of inital activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE