Information on EC 1.1.1.276 - serine 3-dehydrogenase (NADP+)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Search Reference ID:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.1.1.276
-
RECOMMENDED NAME
GeneOntology No.
serine 3-dehydrogenase (NADP+)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-aminomalonate semialdehyde = 2-aminoacetaldehyde + CO2
show the reaction diagram
(1b), spontaneous
-
-
-
L-serine + NADP+ = 2-aminoacetaldehyde + CO2 + NADPH + H+
show the reaction diagram
L-serine + NADP+ = 2-aminomalonate semialdehyde + NADPH + H+
show the reaction diagram
(1a)
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
L-serine:NADP+ 3-oxidoreductase
NAD+ cannot replace NADP+ [cf. EC 1.1.1.387, serine 3-dehydrogenase (NAD+)].
CAS REGISTRY NUMBER
COMMENTARY hide
9038-55-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain ICR 1600
-
-
Manually annotated by BRENDA team
strain JM109
-
-
Manually annotated by BRENDA team
YdfG
-
-
Manually annotated by BRENDA team
YMR226C
-
-
Manually annotated by BRENDA team
YMR226C
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-methyl-DL-serine + NADP+
2-amino-2-methyl-3-oxopropanoic acid + NADPH
show the reaction diagram
3-hydroxypropanoate + NADP+
3-oxopropanoate + NADPH
show the reaction diagram
D-3-hydroxybutyrate + NADP+
3-oxobutyrate + NADPH
show the reaction diagram
D-3-hydroxyisobutyrate + NADP+
2-methyl-3-oxopropanoic acid + NADPH
show the reaction diagram
D-glycerate + NADP+
?
show the reaction diagram
D-serine + NADP+
2-aminomalonate semialdehyde + NADPH
show the reaction diagram
D-threonine + NADP+
D-2-amino-3-oxobutanoic acid + NADPH
show the reaction diagram
L-3-hydroxybutyrate + NADP+
3-oxobutyrate + NADPH
show the reaction diagram
L-3-hydroxyisobutyrate + NADP+
2-methyl-3-oxopropanoic acid + NADPH
show the reaction diagram
L-allo-threonine + NADP+
L-2-amino-3-ketobutyrate + NADPH
show the reaction diagram
L-glycerate + NADP+
?
show the reaction diagram
L-serine + NADP+
2-aminomalonate semialdehyde + NADPH
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-serine + NADP+
2-aminomalonate semialdehyde + NADPH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no effect with Cu2+, Mg2+, Mn2+, Zn2+, Fe2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-methylmalonate
-
competitive against L-serine, noncompetitive against NADP+
D-Cysteine
-
competitive against L-serine, noncompetitive against NADP+
L-cysteine
-
competitive against L-serine, noncompetitive against NADP+
malonate
-
competitive against L-serine, noncompetitive against NADP+
N-ethylmaleimide
-
-
p-chloromercuribenzoate
-
strong
Tartronate
-
competitive against L-serine, noncompetitive against NADP+
additional information
-
no inhibition by EDTA, sodium arsenate, o-phenethroline, alpha,alpha'-dipyridyl and very slight inhibition by sodium azide
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
56
D-glycerate
-
pH 9.0, 30 C
44
D-serine
-
pH 9.0, 30 C
3 - 29
L-allo-threonine
54
L-Glycerate
-
pH 9.0, 30 C
40 - 95
L-serine
0.029 - 0.54
NADP+
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.76 - 43.8
L-allo-threonine
4.2 - 11
L-serine
additional information
additional information
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10 - 12
2-methylmalonate
0.59 - 9.9
D-Cysteine
0.067 - 0.21
L-cysteine
11 - 17
malonate
13 - 18
Tartronate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.043
-
recombinant enzyme in Escherichia coli
2.4
-
purified recombinant enzyme
6
-
purified recombinant enzyme
45.3
-
purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Pyrobaculum calidifontis (strain JCM 11548 / VA1)
Pyrobaculum calidifontis (strain JCM 11548 / VA1)
Pyrobaculum calidifontis (strain JCM 11548 / VA1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
-
gel filtration
105000
-
recombinant enzyme, gel filtration
110000
-
recombinant enzyme, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 10
-
10 min, 40C, stable
639084
7 - 8
-
stable at
639082
7.5 - 10.5
-
10 min, 40C, stable
639084
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
10 min, 0.1 M potassium phosphate, pH 7.4, 0.01% 2-mercaptoethanol, 10% glycerol, stable
55
-
10 min, 0.1 M potassium phosphate, pH 7.4, 0.01% 2-mercaptoethanol, 10% glycerol, stable
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant from Escherichia coli, to homogeneity
-
recombinant from overproducing Escherichia coli, to homogeneity
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis, expression in Escherichia coli JM109
-
overexpression in Escherichia coli JM109
Show AA Sequence (446 entries)
Please use the Sequence Search for a certain query.