Information on EC 1.1.1.276 - serine 3-dehydrogenase (NADP+)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
1.1.1.276
-
RECOMMENDED NAME
GeneOntology No.
serine 3-dehydrogenase (NADP+)
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2-aminomalonate semialdehyde = 2-aminoacetaldehyde + CO2
show the reaction diagram
(1b), spontaneous
-
-
-
L-serine + NADP+ = 2-aminoacetaldehyde + CO2 + NADPH + H+
show the reaction diagram
overall reaction; The product 2-aminomalonate semialdehyde is spontaneously converted into 2-aminoacetaldehyde and CO2. NAD+ cannot replace NADP+
-
-
-
L-serine + NADP+ = 2-aminoacetaldehyde + CO2 + NADPH + H+
show the reaction diagram
sequential mechanism
-
L-serine + NADP+ = 2-aminomalonate semialdehyde + NADPH + H+
show the reaction diagram
(1a)
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
L-serine:NADP+ 3-oxidoreductase
The product 2-ammoniomalonate semialdehyde is spontaneously converted into 2-aminoacetaldehyde and CO2. NAD+ cannot replace NADP+.
CAS REGISTRY NUMBER
COMMENTARY
9038-55-5
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain ICR 1600
-
-
Manually annotated by BRENDA team
Agrobacterium tumefaciens ICR
strain ICR 1600
-
-
Manually annotated by BRENDA team
strain JM109
-
-
Manually annotated by BRENDA team
Escherichia coli JM109
strain JM109
-
-
Manually annotated by BRENDA team
Escherichia coli YdfG
YdfG
-
-
Manually annotated by BRENDA team
Saccharomyces cerevisiae YMR226C
YMR226C
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-methyl-DL-serine + NADP+
2-amino-2-methyl-3-oxopropanoic acid + NADPH
show the reaction diagram
-
59% activity compared to L-serine
-
?
2-methyl-DL-serine + NADP+
2-amino-2-methyl-3-oxopropanoic acid + NADPH
show the reaction diagram
-
8.7% activity compared to L-serine
-
?
2-methyl-DL-serine + NADP+
2-amino-2-methyl-3-oxopropanoic acid + NADPH
show the reaction diagram
-
21% activity compared to L-serine
-
?
3-hydroxypropanoate + NADP+
3-oxopropanoate + NADPH
show the reaction diagram
-
1.6% activity compared to L-serine
-
?
3-hydroxypropanoate + NADP+
3-oxopropanoate + NADPH
show the reaction diagram
-
23% activity compared to L-serine
-
?
3-hydroxypropanoate + NADP+
3-oxopropanoate + NADPH
show the reaction diagram
-
13% activity compared to L-serine
-
?
D-3-hydroxybutyrate + NADP+
3-oxobutyrate + NADPH
show the reaction diagram
-
1% activity compared to L-serine
-
?
D-3-hydroxybutyrate + NADP+
3-oxobutyrate + NADPH
show the reaction diagram
-
12% activity compared to L-serine
-
?
D-3-hydroxyisobutyrate + NADP+
2-methyl-3-oxopropanoic acid + NADPH
show the reaction diagram
-
90% activity compared to L-serine
-
?
D-3-hydroxyisobutyrate + NADP+
2-methyl-3-oxopropanoic acid + NADPH
show the reaction diagram
-
78% activity compared to L-serine
-
?
D-3-hydroxyisobutyrate + NADP+
2-methyl-3-oxopropanoic acid + NADPH
show the reaction diagram
-
3.5% activity compared to L-serine
-
?
D-glycerate + NADP+
?
show the reaction diagram
-
48% activity compared to L-serine
-
-
?
D-glycerate + NADP+
?
show the reaction diagram
-
6% activity compared to L-serine
-
-
?
D-glycerate + NADP+
?
show the reaction diagram
-
12.9% activity compared to L-serine
-
-
?
D-serine + NADP+
2-aminomalonate semialdehyde + NADPH
show the reaction diagram
-
83% activity compared to L-serine
-
?
D-serine + NADP+
2-aminomalonate semialdehyde + NADPH
show the reaction diagram
-
55.2% activity compared to L-serine
-
?
D-serine + NADP+
2-aminomalonate semialdehyde + NADPH
show the reaction diagram
Saccharomyces cerevisiae, Saccharomyces cerevisiae YMR226C
-
71% activity compared to L-serine
-
?
D-serine + NADP+
2-aminomalonate semialdehyde + NADPH
show the reaction diagram
Escherichia coli YdfG
-
83% activity compared to L-serine
-
?
D-threonine + NADP+
D-2-amino-3-oxobutanoic acid + NADPH
show the reaction diagram
-
156% activity compared to L-serine
-
?
D-threonine + NADP+
D-2-amino-3-oxobutanoic acid + NADPH
show the reaction diagram
-
478% activity compared to L-serine
-
?
L-3-hydroxybutyrate + NADP+
3-oxobutyrate + NADPH
show the reaction diagram
-
85% activity compared to L-serine
-
?
L-3-hydroxybutyrate + NADP+
3-oxobutyrate + NADPH
show the reaction diagram
-
9% activity compared to L-serine
-
?
L-3-hydroxyisobutyrate + NADP+
2-methyl-3-oxopropanoic acid + NADPH
show the reaction diagram
-
96% activity compared to L-serine
-
?
L-3-hydroxyisobutyrate + NADP+
2-methyl-3-oxopropanoic acid + NADPH
show the reaction diagram
-
8.5% activity compared to L-serine
-
?
L-3-hydroxyisobutyrate + NADP+
2-methyl-3-oxopropanoic acid + NADPH
show the reaction diagram
-
182% activity compared to L-serine
-
?
L-allo-threonine + NADP+
L-2-amino-3-ketobutyrate + NADPH
show the reaction diagram
-
best substrate
L-2-amino-3-ketobutyrate spontaneously decarboxylates into aminoacetone
?
L-allo-threonine + NADP+
L-2-amino-3-ketobutyrate + NADPH
show the reaction diagram
-
137% activity compared to L-serine
L-2-amino-3-ketobutyrate spontaneously decarboxylates into aminoacetone
?
L-allo-threonine + NADP+
L-2-amino-3-ketobutyrate + NADPH
show the reaction diagram
-
397% activity compared to L-serine
L-2-amino-3-ketobutyrate spontaneously decarboxylates into aminoacetone
?
L-glycerate + NADP+
?
show the reaction diagram
-
28% activity compared to L-serine
-
-
?
L-glycerate + NADP+
?
show the reaction diagram
-
11.3% activity compared to L-serine
-
-
?
L-glycerate + NADP+
?
show the reaction diagram
Saccharomyces cerevisiae, Saccharomyces cerevisiae YMR226C
-
5% activity compared to L-serine
-
-
?
L-glycerate + NADP+
?
show the reaction diagram
Escherichia coli YdfG
-
28% activity compared to L-serine
-
-
?
L-serine + NADP+
2-aminomalonate semialdehyde + NADPH
show the reaction diagram
-
-
-
?
L-serine + NADP+
2-aminomalonate semialdehyde + NADPH
show the reaction diagram
-
-
-
?
L-serine + NADP+
2-aminomalonate semialdehyde + NADPH
show the reaction diagram
-
-
-
?
L-serine + NADP+
2-aminomalonate semialdehyde + NADPH
show the reaction diagram
-
-
-
?
L-serine + NADP+
2-aminomalonate semialdehyde + NADPH
show the reaction diagram
-
best substrate
-
?
L-serine + NADP+
2-aminomalonate semialdehyde + NADPH
show the reaction diagram
-
enzyme belongs to the short-chain alcohol dehydrogenase family, similar reaction mechanism
2-aminomalonate semialdehyde spontaneously decarboxylates into 2-aminoacetaldehyde
?
L-serine + NADP+
2-aminomalonate semialdehyde + NADPH
show the reaction diagram
Saccharomyces cerevisiae YMR226C, Escherichia coli YdfG
-
-
-
?
L-serine + NADP+
2-aminomalonate semialdehyde + NADPH
show the reaction diagram
Escherichia coli JM109, Agrobacterium tumefaciens ICR
-
-
-
?
L-serine + NADP+
2-aminomalonate semialdehyde + NADPH
show the reaction diagram
Agrobacterium tumefaciens ICR
-
enzyme belongs to the short-chain alcohol dehydrogenase family, similar reaction mechanism
2-aminomalonate semialdehyde spontaneously decarboxylates into 2-aminoacetaldehyde
?
additional information
?
-
-
no activity with DL-homoserine, O-methyl-L-serine, N-acetyl-DL-serine, DL-isoserine, DL-threo-3-phenylserine, malonate, DL-malate, DL-lactate, DL-tartrate, D-gluconate, citrate, isocitrate, glycolate, glycerol
-
-
-
additional information
?
-
-
no activity with 4-hydroxybutyrate, DL-2-hydroxybutyrate, DL-2-hydroxyisobutyrate, DL-2-hydroxycaproate, DL-2-hydroxyisocaproate, 3-phosphoglycerate, DL-lactate, L-malate, glycollate, glycerol, D-threonine, DL-threo-phenylserine, DL-homoserine, DL-isoserine, L-alanine, beta-alanine, L-aspartate, L-glutamate, L-valine, L-leucine, L-methionine, L-lysine, L-phenylalanine, and glycine
-
-
-
additional information
?
-
-
L-threonine, D-allo-threonine are poor substrates
-
-
-
additional information
?
-
Saccharomyces cerevisiae YMR226C
-
no activity with DL-homoserine, O-methyl-L-serine, N-acetyl-DL-serine, DL-isoserine, DL-threo-3-phenylserine, malonate, DL-malate, DL-lactate, DL-tartrate, D-gluconate, citrate, isocitrate, glycolate, glycerol, L-threonine, D-allo-threonine are poor substrates
-
-
-
additional information
?
-
Escherichia coli YdfG
-
no activity with DL-homoserine, O-methyl-L-serine, N-acetyl-DL-serine, DL-isoserine, DL-threo-3-phenylserine, malonate, DL-malate, DL-lactate, DL-tartrate, D-gluconate, citrate, isocitrate, glycolate, glycerol, L-threonine, D-allo-threonine are poor substrates
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-serine + NADP+
2-aminomalonate semialdehyde + NADPH
show the reaction diagram
-
-
-
?
L-serine + NADP+
2-aminomalonate semialdehyde + NADPH
show the reaction diagram
-
-
-
?
L-serine + NADP+
2-aminomalonate semialdehyde + NADPH
show the reaction diagram
-
-
-
?
L-serine + NADP+
2-aminomalonate semialdehyde + NADPH
show the reaction diagram
-
-
-
?
L-serine + NADP+
2-aminomalonate semialdehyde + NADPH
show the reaction diagram
Saccharomyces cerevisiae YMR226C, Escherichia coli YdfG
-
-
-
?
L-serine + NADP+
2-aminomalonate semialdehyde + NADPH
show the reaction diagram
Escherichia coli JM109, Agrobacterium tumefaciens ICR
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NADP+
-
dependent on; NAD+ cannot replace NADP+
NADP+
-
dependent on; NAD+ cannot replace NADP+
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
no effect with Cu2+, Mg2+, Mn2+, Zn2+, Fe2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-methylmalonate
-
competitive against L-serine, noncompetitive against NADP+
D-Cysteine
-
competitive against L-serine, noncompetitive against NADP+
L-cysteine
-
competitive against L-serine, noncompetitive against NADP+
N-ethylmaleimide
-
-
p-chloromercuribenzoate
-
strong
Tartronate
-
competitive against L-serine, noncompetitive against NADP+
malonate
-
competitive against L-serine, noncompetitive against NADP+
additional information
-
no inhibition by EDTA, sodium arsenate, o-phenethroline, alpha,alpha'-dipyridyl and very slight inhibition by sodium azide
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
56
D-glycerate
-
pH 9.0, 30 C
44
D-serine
-
pH 9.0, 30 C
3
L-allo-threonine
-
pH 9.0, 30C
29
L-allo-threonine
-
pH 9.0, 30C
54
L-Glycerate
-
pH 9.0, 30 C
40
L-serine
-
pH 9.0, 30C
42
L-serine
-
pH 9.0, 30 C
0.029
NADP+
-
pH 9.0, 30C
0.5
NADP+
-
pH 9.0, 30C
0.54
NADP+
-
pH 9.0, 30C
95
L-serine
-
pH 9.0, 30C
additional information
additional information
-
substrate specificity
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
5.76
L-allo-threonine
Escherichia coli
-
pH 9.0, 30C
43.8
L-allo-threonine
Saccharomyces cerevisiae
-
pH 9.0, 30C
4.2
L-serine
Escherichia coli
-
pH 9.0, 30C
11
L-serine
Saccharomyces cerevisiae
-
pH 9.0, 30C
additional information
additional information
Escherichia coli, Saccharomyces cerevisiae
-
substrate specificity
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
10
2-methylmalonate
-
versus NADP+, pH 9.0, 30C
12
2-methylmalonate
-
versus L-serine, pH 9.0, 30C
0.59
D-Cysteine
-
versus L-serine, pH 9.0, 30C
9.9
D-Cysteine
-
versus NADP+, pH 9.0, 30C
0.067
L-cysteine
-
versus L-serine, pH 9.0, 30C
0.21
L-cysteine
-
versus NADP+, pH 9.0, 30C
11
malonate
-
versus L-serine, pH 9.0, 30C
17
malonate
-
versus NADP+, pH 9.0, 30C
13
Tartronate
-
versus L-serine, pH 9.0, 30C
18
Tartronate
-
versus NADP+, pH 9.0, 30C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.043
-
recombinant enzyme in Escherichia coli
2.4
-
purified recombinant enzyme
6
-
purified recombinant enzyme
45.3
-
purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
8.5
-
oxidation of L-serine
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
-
assay at
PDB
SCOP
CATH
ORGANISM
UNIPROT
Pyrobaculum calidifontis (strain JCM 11548 / VA1)
Pyrobaculum calidifontis (strain JCM 11548 / VA1)
Pyrobaculum calidifontis (strain JCM 11548 / VA1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
100000
-
gel filtration
639082
105000
-
recombinant enzyme, gel filtration
639084
110000
-
recombinant enzyme, gel filtration
639084
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
tetramer
-
x * 25000-26600, recombinant enzyme, SDS-PAGE and amino acid sequence determination
tetramer
-
-
tetramer
-
4 * 27000, recombinant enzyme, SDS-PAGE
tetramer
-
4 * 29000, recombinant enzyme, SDS-PAGE
tetramer
Agrobacterium tumefaciens ICR
-
x * 25000-26600, recombinant enzyme, SDS-PAGE and amino acid sequence determination
-
tetramer
Escherichia coli JM109
-
-
-
tetramer
Escherichia coli YdfG
-
4 * 27000, recombinant enzyme, SDS-PAGE
-
tetramer
Saccharomyces cerevisiae YMR226C
-
4 * 29000, recombinant enzyme, SDS-PAGE
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5 - 10
-
10 min, 40C, stable
639084
7 - 8
-
stable at
639082
7.5 - 10.5
-
10 min, 40C, stable
639084
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
35
-
stable at
639082
40
-
10 min, 0.1 M potassium phosphate, pH 7.4, 0.01% 2-mercaptoethanol, 10% glycerol, stable
639084
55
-
10 min, 0.1 M potassium phosphate, pH 7.4, 0.01% 2-mercaptoethanol, 10% glycerol, stable
639084
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant from Escherichia coli, to homogeneity
-
recombinant from overproducing Escherichia coli, to homogeneity
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis, expression in Escherichia coli JM109
-
overexpression in Escherichia coli JM109
-