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Information on EC 1.1.1.270 - 3beta-hydroxysteroid 3-dehydrogenase and Organism(s) Mus musculus and UniProt Accession O88736

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IUBMB Comments
The enzyme acts on multiple 3beta-hydroxysteroids. Participates in the biosynthesis of zemosterol and cholesterol, where it catalyses the reaction in the opposite direction to that shown. The mammalian enzyme is bifunctional and also catalyses EC 1.1.1.62, 17beta-estradiol 17-dehydrogenase .
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This record set is specific for:
Mus musculus
UNIPROT: O88736
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
3beta-hydroxysteroid dehydrogenase, hsd3b1, akr1c2, hsd17b7, erg27, 3beta-hsd1, 3-ketosteroid reductase, 3beta-hsd2, 3-ketoreductase, erg27p, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17beta-hydroxysteroid dehydrogenase type 7
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3-keto reducing enzyme
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3-keto reductase
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-
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3-ketoreductase
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3-ketosteroid reductase
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-
-
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3-KSR
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-
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3-oxo steroid reductase
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3-oxosteroid reductase
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-
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HSD17B7
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reductase, 3-oxo steroid
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steroid-3-ketoreductase
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-
-
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sterone-reducing enzyme
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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-
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PATHWAY SOURCE
PATHWAYS
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-, -, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
3beta-hydroxysteroid:NADP+ 3-oxidoreductase
The enzyme acts on multiple 3beta-hydroxysteroids. Participates in the biosynthesis of zemosterol and cholesterol, where it catalyses the reaction in the opposite direction to that shown. The mammalian enzyme is bifunctional and also catalyses EC 1.1.1.62, 17beta-estradiol 17-dehydrogenase [4].
CAS REGISTRY NUMBER
COMMENTARY hide
42616-29-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
17beta-hydroxy-5alpha-androstan-3-one + NADPH
5alpha-androstane-3beta,17beta-diol + NADP+
show the reaction diagram
zymosterone + NADPH
zymosterol + NADP+
show the reaction diagram
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-
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-
?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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high expression levels in the ganglion of the trigeminal nerve and the dorsal root ganglia
Manually annotated by BRENDA team
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of the trigeminal nerve, dorsal root ganglia, cranial ganglia
Manually annotated by BRENDA team
additional information
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specifically expressed in tissues that are involved in the pathogenesis of congenital cholesterol-deficiency disorders
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DHB7_MOUSE
334
1
37317
Swiss-Prot
Secretory Pathway (Reliability: 5)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
2 h at 25°C: 84% of activity remained, 8 h at 25°C: 74-76% of activity remained
additional information
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of HSD17B7 as a glutathione-S-transferase fusion protein in Escherichia coli. Erg27p-deficient yeast strain complements the 3-ketosteroid reductase deficiency of the cells and restores growth on sterol-deficient medium
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wieland, S.J.; Fox, T.O.
A DNA-binding of mouse kidney 3-ketosteroid reductase: comparison with androgen receptors
Steroids
37
527-538
1981
Mus musculus
Manually annotated by BRENDA team
Marijanovic, Z.; Laubner, D.; Moeller, G.; Gege, C.; Husen, B.; Adamski, J.; Breitling, R.
Closing the gap: Identification of human 3-ketosteroid reductase, the last unknown enzyme of mammalian cholesterol biosynthesis
Mol. Endocrinol.
17
1715-1725
2003
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Taramino, S.; Teske, B.; Oliaro-Bosso, S.; Bard, M.; Balliano, G.
Divergent interactions involving the oxidosqualene cyclase and the steroid-3-ketoreductase in the sterol biosynthetic pathway of mammals and yeasts
Biochim. Biophys. Acta
1801
1232-1237
2010
Homo sapiens (P56937), Homo sapiens, Mus musculus (O88736), Mus musculus
Manually annotated by BRENDA team
Layer, J.; Barnes, B.; Yamasaki, Y.; Barbuch, R.; Li, L.; Taramino, S.; Balliano, G.; Bard, M.
Characterization of a mutation that results in independence of oxidosqualene cyclase (Erg7) activity from the downstream 3-ketoreductase (Erg27) in the yeast ergosterol biosynthetic pathway
Biochim. Biophys. Acta
1831
361-369
2013
Mus musculus (O88736), Mus musculus
Manually annotated by BRENDA team