Information on EC 1.1.1.270 - 3beta-hydroxysteroid 3-dehydrogenase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY hide
1.1.1.270
-
RECOMMENDED NAME
GeneOntology No.
3beta-hydroxysteroid 3-dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a 3beta-hydroxysteroid + NADP+ = a 3-oxosteroid + NADPH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
oxidation of 3beta-hydroxy-steroids poorly catalyzed for a few substrates
-
redox reaction
-
-
-
-
reduction
-
-
ketone reduction
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
-
-
cholesterol biosynthesis
-
-
cholesterol biosynthesis I
-
-
cholesterol biosynthesis II (via 24,25-dihydrolanosterol)
-
-
cholesterol biosynthesis III (via desmosterol)
-
-
Metabolic pathways
-
-
Steroid biosynthesis
-
-
zymosterol biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
3beta-hydroxysteroid:NADP+ 3-oxidoreductase
The enzyme acts on multiple 3beta-hydroxysteroids. Participates in the biosynthesis of zemosterol and cholesterol, where it catalyses the reaction in the opposite direction to that shown. The mammalian enzyme is bifunctional and also catalyses EC 1.1.1.62, 17beta-estradiol 17-dehydrogenase [4].
CAS REGISTRY NUMBER
COMMENTARY hide
42616-29-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene erg27
-
-
Manually annotated by BRENDA team
var. neoformans, B42419, ATCC 32265, pathogenic fungus
-
-
Manually annotated by BRENDA team
zebra finch
-
-
Manually annotated by BRENDA team
maize, variety LG11
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(3)H-dehydroepiandrosterone + NAD(P)+
?
show the reaction diagram
-
-
-
-
?
16alpha-hydroxy-dehydroepiandrosterone + NADH + H+
?
show the reaction diagram
-
-
-
-
?
16beta-hydroxy-dehydroepiandrosterone + NADH + H+
?
show the reaction diagram
-
-
-
-
?
17alpha-hydroxypregnenolone + NADH + H+
17alpha-hydroxyprogesterone + NAD+
show the reaction diagram
-
-
-
-
?
17beta-hydroxy-5alpha-androstan-3-one + NADPH
5alpha-androstane-3beta,17beta-diol + NADP+
show the reaction diagram
2 17beta-hydroxy-5alpha-androstan-3-one + 2 NADPH + 2 H+
5alpha-androstane-3beta,17beta-diol + 5alpha-androstane-3alpha,17beta-diol + 2 NADP+
show the reaction diagram
24-ethylidenelophenone + NADPH
24-ethylidenelophenol + NADP+
show the reaction diagram
-
oxidation: poor
-
r
24-methylenecycloartanone + NADPH
24-methylenecycloartanol + NADP+
show the reaction diagram
-
7% compared with cholest-7-en-3-one, DELTA7-cholestenone
-
ir
24-methylenelophenone + NADPH
24-methylenelophenol + NADP+
show the reaction diagram
-
35% compared with cholest-7-en-3-one, DELTA7-cholestenone
-
?
24-methylenepollinastanone + NADPH + acetate
24-methylenepollinastanol + NADP+
show the reaction diagram
-
70% compared with cholest-7-en-3-one, DELTA7-cholestenone
-
?
24RS-dihydrocycloeucalenone + NADPH
24RS-dihydrocycloeucalenol + NADP+
show the reaction diagram
-
53% compared with cholest-7-en-3-one, DELTA7-cholestenone
-
?
3-keto-5alpha-androstane steroids + NADPH
3beta-hydroxy-5alpha-androstane steroids + NADP+
show the reaction diagram
-
-
-
ir
3alpha-androstanediol + NAD+
5alpha-dihydrotestosterone + NADH
show the reaction diagram
-
-
-
-
?
3alpha-androstanediol + NAD+
? + NADH
show the reaction diagram
AKR1C2 and AKR1C4 act as 3alpha-hydroxysteroid oxidase, AKR1C3 predominantly acts as 17beta-hydroxysteroid oxidase catalyzing the conversion of 3alpha-diol to androsterone, negligible activity with the 3beta-androstanediol
-
-
?
4,4-gem-dimethyl-5alpha-cholest-7-en-3-one + NADPH
4,4-gem-dimethyl-5alpha-cholest-7-en-3beta-ol + NADP+
show the reaction diagram
4alpha-methyl-5alpha-cholest-7-en-3-one + NADPH
4alpha-methyl-5alpha-cholest-7-en-3beta-ol + NADP+
show the reaction diagram
-
best substrate, reduction consistently about two times greater for monosubstituted steroid
-
?
4alpha-methylfecosterone + NADPH + H+
4alpha-methylfecosterol + NADP+
show the reaction diagram
-
-
4alpha-methylfecosterol is further converted to ergosterol
-
?
4alpha-methylzymosterol + NADP+
3-dehydro-4-methylzymosterol + NADPH + H+
show the reaction diagram
-
-
-
-
?
4beta-methyl-28-nor-24RS-dihydrocycloeucalenone + NADPH
4beta-methyl-28-nor-24RS-dihydrocycloeucalenol + NADP+
show the reaction diagram
-
27% compared with cholest-7-en-3-one, DELTA7-cholestenone
-
?
5-androstene-3,17-dione + NAD+
dehydroepiandrosterone + NADH + H+
show the reaction diagram
-
-
-
-
?
5alpha-androstan-3,17-dione + NADPH + H+
5alpha-androstan-3beta-ol-17-one + NADP+
show the reaction diagram
-
-
-
-
r
5alpha-androstane-3,17-dione + NADPH
epi-androsterone + NADP+
show the reaction diagram
5alpha-dihydrotestosterone + NADPH
3alpha-androstanediol + NADP+
show the reaction diagram
5alpha-dihydrotestosterone + NADPH
5alpha-androstan-3alpha,17beta-diol + NADP+
show the reaction diagram
-
i.e. DHT, reductive 3alpha-HSD activity of AKR1C1 yielding the 3alpha,17-diol, low activity
-
-
?
5alpha-dihydrotestosterone + NADPH
5alpha-androstan-3beta,17beta-diol + NADP+
show the reaction diagram
-
i.e. DHT, reductive 3beta-HSD activity of AKR1C1 yielding the 3beta,17-diol, preferred reaction
-
-
?
5alpha-dihydrotestosterone + NADPH + H+
3beta,17beta-dihydroxy-5alpha-androstane + NADP+
show the reaction diagram
-
-
-
-
r
5alpha-pregnan-3,20-dione + NADPH + H+
5alpha-pregnane-3beta-ol-20-one + NADP+
show the reaction diagram
-
-
-
-
r
5alpha-pregnane-21-ol-3,20-dione + NADPH + H+
5alpha-pregnane-3beta,21-diol-20-one + NADP+
show the reaction diagram
-
-
-
-
r
5beta-androstan-3,17-dione + NADPH + H+
5beta-androstan-3beta-ol-17-one + NADP+
show the reaction diagram
-
-
-
-
r
5beta-androstan-3beta-ol-17-one + NADP+
5beta-androstan-3,17-dione + NADPH + H+
show the reaction diagram
-
-
-
-
r
5beta-androstane-3beta,17beta-diol + NADP+
5beta-androstane-17beta-ol-3-one + NADPH + H+
show the reaction diagram
-
-
-
-
r
5beta-cholanic acid-3,7-dione + NADPH + H+
5beta-cholanic acid-3beta-ol-7-one + NADP+
show the reaction diagram
-
-
-
-
r
5beta-dihydrocortisone + NADPH + H+
?
show the reaction diagram
-
-
-
-
r
5beta-dihydrocortisosterone + NADPH + H+
?
show the reaction diagram
-
-
-
-
r
5beta-dihydrotestosterone + NADPH + H+
3beta,17beta-dihydroxy-5beta-androstane + NADP+
show the reaction diagram
-
-
-
-
r
5beta-hydroxy-5beta cholanic acid + NADP+
3-oxo-5-beta-cholanic acid + NADPH + H+
show the reaction diagram
-
-
-
-
r
5beta-pregnan-3,20-dione + NADPH + H+
5beta-pregnane-3beta-ol-20-one + NADP+
show the reaction diagram
-
-
-
-
r
5beta-pregnane-20-ol-3-one + NADPH + H+
5beta-pregnane-3beta,20-diol + NADP+
show the reaction diagram
-
-
-
-
r
5beta-pregnane-21-ol-3,20-dione + NADPH + H+
5beta-pregnane-3beta,21-diol-20-one + NADP+
show the reaction diagram
-
-
-
-
r
5beta-pregnane-3beta,20alpha-diol + NADP+
5beta-pregnane-20alpha-ol-3-one + NADPH + H+
show the reaction diagram
-
-
-
-
r
5beta-pregnane-3beta,20beta-diol + NADP+
5beta-pregnane-20beta-ol-3-one + NADPH + H+
show the reaction diagram
-
-
-
-
r
5beta-pregnane-3beta,21-diol-20-one + NADP+
5beta-pregnane-21-ol-3,20-dione + NADPH + H+
show the reaction diagram
-
-
-
-
r
5beta-pregnane-3beta-ol-20-one + NADP+
5beta-pregnan-3,20-dione + NADPH + H+
show the reaction diagram
-
-
-
-
r
androstenedione + NAD(P)H
?
show the reaction diagram
-
-
-
-
?
avenastenone + NADPH
avenasterol + NADP+
show the reaction diagram
-
more than 72% compared with cholest-7-en-3-one, DELTA7-cholestenone
-
?
cholest-7-en-3-one + NADPH
3beta-hydroxy-cholest-7-ene + NADP+
show the reaction diagram
-
DELTA7-cholestenone, best substrate
DELTA7-cholesterol
ir
cholestanone + NADPH
cholestanol + NADP+
show the reaction diagram
-
57% compared with cholest-7-en-3-one, DELTA7-cholestenone
-
?
cycloartenone + NADPH
cycloartenol + NADP+
show the reaction diagram
-
7% compared with cholest-7-en-3-one, DELTA7-cholestenone
-
ir
cycloeucalenone + NADPH + H+
cycloeucalenol + NADP+
show the reaction diagram
cyclolaudenone + NADPH
cyclolaudenol + NADP+
show the reaction diagram
-
10% compared with cholest-7-en-3-one, DELTA7-cholestenone
-
?
dehydroepiandrosterone + NADH + H+
5-androstene-3,17-dione + NAD+
show the reaction diagram
-
-
-
-
?
dehydrolithocholic acid + NADPH + H+
?
show the reaction diagram
-
-
-
-
r
DELTA7-chondrillastenone + NADPH
DELTA7-chondrillasterol + NADP+
show the reaction diagram
farnesol + NADP+
farnesal + NADPH + H+
show the reaction diagram
-
-
-
-
?
fecosterone + NADPH + H+
fecosterol + NADP+
show the reaction diagram
-
-
-
-
?
geranylgeraniol + NADP+
geranylgeranial + NADPH + H+
show the reaction diagram
-
-
-
-
?
isolithocholic acid + NADP+
? + NADPH + H+
show the reaction diagram
-
-
-
-
r
methylglyoxal + NADP+
? + NADPH + H+
show the reaction diagram
-
-
-
-
?
obtusifolione + NADPH
obtusifoliol + NADP+
show the reaction diagram
pregnenolone + NADH + H+
progesterone + NAD+
show the reaction diagram
-
-
-
-
?
progesterone + NADPH
pregn-4-ene-20alpha-ol-3-one + NADP+
show the reaction diagram
-
i.e. pregn-4-ene-3,20-dione
-
-
?
pyridine-3-aldehyde + NADP+
pyridin-3-ylmethanol + NADPH + H+
show the reaction diagram
-
-
-
-
?
spinastenone + NADPH
spinasterol + NADP+
show the reaction diagram
-
32% compared with cholest-7-en-3-one, DELTA7-cholestenone
-
?
zymosterone + NADPH
zymosterol + NADP+
show the reaction diagram
[7alpha,17alpha]-17-hydroxy-7-methyl-19-norpregn-5(10)-en-20-yn-3-one + NADPH
[7alpha,17alpha]-17-hydroxy-7-methyl-19-norpregn-5(10)-en-20-yn-3-ol + NADP+
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(3)H-dehydroepiandrosterone + NAD(P)+
?
show the reaction diagram
-
-
-
-
?
2 17beta-hydroxy-5alpha-androstan-3-one + 2 NADPH + 2 H+
5alpha-androstane-3beta,17beta-diol + 5alpha-androstane-3alpha,17beta-diol + 2 NADP+
show the reaction diagram
Q04828
AKR1cs are a source of beta-tetrahydrosteroids. This is of physiological significance because the formation of 3beta-diol in contrast to 3alpha-diol is virtually irreversible, the 3beta-diol is a pro-apoptotic ligand for estrogen receptor beta, and 3beta-tetrahydrosteroids act as gamma-aminobutyric acid type A receptor antagonists
-
-
?
5alpha-dihydrotestosterone + NADPH
3alpha-androstanediol + NADP+
show the reaction diagram
-
in prostate cells AKR1C2 acts as a 3-ketosteroid reductase to eliminate 5alpha-dihydrotestosterone and prevents activation of androgen receptor. AKR1C2 does not act as an oxidase due to either potent product inhibition by NADPH or because it cannot surmount the oxidative 17beta-hydroxysteroid dehydrogenase present. AKR1C2 is not a source of 5alpha-dihydrotestosterone in PC-3 cells
-
-
?
androstenedione + NAD(P)H
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
-
-
additional information
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(Z)-2-(4-methoxyphenylimino)-7-hydroxy-N-(pyridin-2-yl)-2H-chromene-3-carboxamide
-
noncompetitive in the reduction reaction, competitive in the oxidation reaction
3-(4-hydroxy-2-methoxyphenyl)acrylic acid 3-(3-hydroxyphenyl)propyl ester
-
uncompetitive in the reduction reaction, competitive in the oxidation reaction
5beta-cholanic acid-3alpha,7alpha-diol
-
-
acetonitrile
-
-
bisdemethoxycurcumin
-
-
diethylstilbestrol
-
-
dimethyl sulfoxide
-
-
Diphenic acid
-
competitive in the oxidation reaction
Epalrestat
-
-
ethanol
-
-
fenhexamid
-
Erg27 mutations causing amino acid substitutions in or near the transmembrane domain strongly decrease the affinity of fenhexamid for 3-ketoreductase. Sterol 3-ketoreductase sensitivity to fenhexamid in the various fungal strains, overview
Flufenamic acid
Ionic detergents
-
inactivation by strong solutions of ionic detergents, ethylene glycol minimizes inactivation
-
itraconazole
minalrestat
-
competitive in the oxidation reaction
oleanolic acid
-
competitive in the oxidation reaction
propan-2-ol
-
-
Tolrestat
-
competitive in the oxidation reaction
trilostane
zopolrestat
-
-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytosolic Z protein
-
fatty acid-binding protein, membrane-bound enzyme, twofold to threefold activation, stimulatory activity is lost after solubilization of microsomal enzyme. Stimulation reversed by titration of Z-protein with either fatty acids or anti-Z-protein immunoglobulin. Stimulation not restored by incorporating partially purified reductase into an artificial phospholipid membrane
-
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0043
17beta-hydroxy-5alpha-androstan-3-one
-
pH 7.0, 37C, spectrophotometric analysis, formation of 3beta-androstanediol or 3alpha-androstanediol
0.085
24-methylenecycloartanone
-
-
0.162
24-methylenelophenone
-
-
0.078 - 0.236
4alpha-methyl-5alpha-cholest-7-en-3-one
0.0035 - 0.039
5beta-dihydrotestosterone
0.0023 - 0.05
5beta-pregnane-21-ol-3,20-dione
0.22
cholestanone
-
-
0.067
cycloartenone
-
-
0.22
cycloeucalenone
-
-
0.1
cyclolaudenone
-
-
0.42
DELTA7-cholestenone
-
-
0.245
DELTA7-chondrillastenone
-
-
-
0.18 - 0.253
methylglyoxal
0.00012 - 0.004
NADPH
0.26
obtusifolione
-
-
0.011 - 0.048
Pyridine-3-aldehyde
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.003 - 0.052
17beta-hydroxy-5alpha-androstan-3-one
0.02 - 0.113
5beta-dihydrotestosterone
0.023 - 0.092
5beta-pregnane-21-ol-3,20-dione
0.3 - 0.58
methylglyoxal
0.73
NADPH
Oryctolagus cuniculus
-
pH 7.4, 25C, wild-type enzyme
0.6 - 1.2
Pyridine-3-aldehyde
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0001 - 0.00256
trilostane
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000008
(Z)-2-(4-methoxyphenylimino)-7-hydroxy-N-(pyridin-2-yl)-2H-chromene-3-carboxamide
Oryctolagus cuniculus
-
pH 7.4, 25C, wild-type enzyme
0.00006
3-(4-hydroxy-2-methoxyphenyl)acrylic acid 3-(3-hydroxyphenyl)propyl ester
Oryctolagus cuniculus
-
pH 7.4, 25C, wild-type enzyme
0.0017
AL1567
Oryctolagus cuniculus
-
pH 7.4, 25C, wild-type enzyme
0.0006
bisdemethoxycurcumin
Oryctolagus cuniculus
-
pH 7.4, 25C, wild-type enzyme
0.0028
diethylstilbestrol
Oryctolagus cuniculus
-
pH 7.4, 25C, wild-type enzyme
0.26
Diphenic acid
Oryctolagus cuniculus
-
pH 7.4, 25C, wild-type enzyme
0.0015
Epalrestat
Oryctolagus cuniculus
-
pH 7.4, 25C, wild-type enzyme
0.000043
minalrestat
Oryctolagus cuniculus
-
pH 7.4, 25C, wild-type enzyme
0.0004
oleanolic acid
Oryctolagus cuniculus
-
pH 7.4, 25C, wild-type enzyme
0.0015
quercetin
Oryctolagus cuniculus
-
pH 7.4, 25C, wild-type enzyme
0.0053
Sorbinil
Oryctolagus cuniculus
-
pH 7.4, 25C, wild-type enzyme
0.00086
sulindac
Oryctolagus cuniculus
-
pH 7.4, 25C, wild-type enzyme
0.00016
Tolrestat
Oryctolagus cuniculus
-
pH 7.4, 25C, wild-type enzyme
0.00023
trilostane
Rattus norvegicus
-
IC50: 0.00023 mM
0.0012
zopolrestat
Oryctolagus cuniculus
-
pH 7.4, 25C, wild-type enzyme
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0014
-
partially purified enzyme
0.0039
-
with substrate 5alpha-dihydrotestosterone, formation of the 3alpha-hydroxysteroid
0.0161
-
with substrate 5alpha-dihydrotestosterone, formation of the 3beta-hydroxysteroid
0.0312
-
with substrate tibolone
0.0432
-
with substrate progesterone
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
assay at
7.5
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27
-
assay at
30
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
selective expression of 3beta-HSD1
Manually annotated by BRENDA team
-
of the trigeminal nerve, dorsal root ganglia, cranial ganglia
Manually annotated by BRENDA team
-
primary, time-dependent reduction of tibolone into 3beta- and 3alpha-hydroxytibolone was observed again in a 4:1 ratio
Manually annotated by BRENDA team
-
selective expression of 3beta-HSD1
Manually annotated by BRENDA team
-
AKR1C2 is not a source of 5alpha-dihydrotestosterone in PC-3 cells
Manually annotated by BRENDA team
-
selective expression of 3beta-HSD1
Manually annotated by BRENDA team
-
in primary cultures of epithelial cells, high levels of AKR1C2 transcripts are detected in prostate cancer, but not in cell from normal prostate. In prostate cells AKR1C2 acts as a 3-ketosteroid reductase to eliminate 5alpha-dihydrotestosterone and prevents activation of androgen receptor
Manually annotated by BRENDA team
-
in primary cultures of epithelial cells, high levels of AKR1C2 transcripts are detected in prostate cancer, but not in cell from normal prostate
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
-
native enzyme, gel filtration
additional information
-
chromatography on Sepharose 2B column shows ketoreductase activity eluted as high-molecular-weight complex
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
2 h at 25C: 84% of activity remained, 8 h at 25C: 74-76% of activity remained
additional information
-
-
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme extreme labile
-
enzyme stable in presence of high concentrations of detergents
-
ethylene glycol stabilizes
-
stable against subtilisin VII, no attendent loss of 3-ketoreductase activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme from lungs by ammonium sufate fractionation, gel filtration, and anion exchange chromatography, recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography and ultrafiltration
-
recombinant wild-type and mutant enzymes from Sf9 insect cells by ultracentrifugation
-
solubilization with Lubrol-WX in the presence of cholic acid, and partial purification
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
3-ketosteroid reductase activity of the human 3(alpha-beta)-hydroxysteroid epimerase, stable expression
-
DNA and amino acid sequence determination and analysis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) pLysS
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expression of HSD17B7 and truncated HSD17B7 (shortened by 127 amino acids at the C terminus) as glutathione-S-transferase fusion protein in Escherichia coli. Erg27p-deficient yeast strain complements the 3-ketosteroid reductase deficiency of the cells and restores growth on sterol-deficient medium
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expression of HSD17B7 as a glutathione-S-transferase fusion protein in Escherichia coli. Erg27p-deficient yeast strain complements the 3-ketosteroid reductase deficiency of the cells and restores growth on sterol-deficient medium
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expression of wild-type and mutant enzymes in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system
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isoform type III of 3beta-hydroxysteroid dehydrogenase/DELTA5-DELTA4 isomerase with almost exclusive 3-ketosteroid reductase activity, cDNA expression
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overexpression in Escherichia coli
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quantitative expression analysis of AKR1C isozymes
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D61N
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site-directed mutagenesis, mutation in isozyme 3beta-HSD1
D61V
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site-directed mutagenesis, mutation in isozyme 3beta-HSD1
DELTA214-341
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inactive mutant enzyme
E192A
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site-directed mutagenesis, mutation in isozyme 3beta-HSD1
P195R
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site-directed mutagenesis, mutation in isozyme 3beta-HSD2
R195P
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site-directed mutagenesis, mutation in isozyme 3beta-HSD1
T8A
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site-directed mutagenesis, mutation in isozyme 3beta-HSD1
F303Q
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site-directed mutagenesis, the enzyme shows reduced 3-ketoreductase activity compared to the wild-type enzyme
F303Q/M304S
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site-directed mutagenesis, the enzyme shows reduced 3-ketoreductase activity compared to the wild-type enzyme. The double mutation impairs the affinity and catalytic efficiency, although it did not affect the stereospecific reduction of the two 3-ketosteroids into the corresponding 3beta-hydroxysteroids
L116F
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site-directed mutagenesis
M304S
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site-directed mutagenesis, the enzyme shows reduced 3-ketoreductase activity compared to the wild-type enzyme. The M304S mutation causes a 4fold increase in the Km value for pyridine-3-aldehyde
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
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enzyme can be used for reconstitution of 4-methyl sterol demethylations of cholesterol biosynthesis from lanosterol
medicine
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the selective inhibition of human 3beta-HSD1 in breast tumors represents a potential treatment for hormone-sensitive breast cancer