Information on EC 1.1.1.264 - L-idonate 5-dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.1.1.264
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RECOMMENDED NAME
GeneOntology No.
L-idonate 5-dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-idonate + NAD(P)+ = 5-dehydro-D-gluconate + NAD(P)H + H+
show the reaction diagram
The enzyme from E. coli cannot oxidize D-gluconate to form 5-dehydrogluconate, a reaction that is catalysed by EC 1.1.1.69, gluconate 5-dehydrogenase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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reduction
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-idonate degradation
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ketogluconate metabolism
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SYSTEMATIC NAME
IUBMB Comments
L-idonate:NAD(P)+ oxidoreductase
The enzyme from the bacterium Escherichia coli is specific for 5-dehydro-D-gluconate. cf. EC 1.1.1.366, L-idonate 5-dehydrogenase (NAD+).
CAS REGISTRY NUMBER
COMMENTARY hide
211737-68-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
K-12 strains, idnD gene
Uniprot
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain no. 125
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Manually annotated by BRENDA team
Gluconobacter gluconicus
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-
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Manually annotated by BRENDA team
no activity in Ampelopsis aconitifolia
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
cv. Cabernet Sauvignon
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-keto-D-fructose + NADP+
? + NADPH
show the reaction diagram
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low activity
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?
D-gluconate + NADP+
? + NADPH
show the reaction diagram
-
-
-
r
L-idonate + NAD(P)+
5-dehydrogluconate + NAD(P)H
show the reaction diagram
specific oxidation of L-idonate with NAD+ as cofactor, involved in the degradation of L-idonate to form D-gluconate
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r
L-idonate + NADP+
5-dehydro-D-gluconate + NADPH
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-idonate + NAD(P)+
5-dehydrogluconate + NAD(P)H
show the reaction diagram
P39346
specific oxidation of L-idonate with NAD+ as cofactor, involved in the degradation of L-idonate to form D-gluconate
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r
L-idonate + NADP+
5-dehydro-D-gluconate + NADPH
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
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slight activation at 1 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
p-chloromercuribenzoate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
12.5
5-Dehydro-D-gluconate
pH 8.5, 30°C, recombinant enzyme
0.9 - 1.1
5-Keto-D-gluconate
20
D-gluconate
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1 - 2.2
L-Idonate
0.02
NADP+
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0.006
NADPH
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.04
with NADPH as cofactor
0.05
with NADH as cofactor
additional information
in vivo activity, feeding experiments, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
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5-keto-D-gluconate reduction
6.5 - 7.4
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L-idonate formation
10
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D-gluconate oxidation
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000
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4 * 25000, SDS-PAGE
110000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
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4 * 25000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
55% ammonium sulfate precipitation
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 6
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80% of maximal activity within
285823
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
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complete inactivation within 5 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
highly unstable, 75% loss of activity within a few days, stabilizing agents during purification: D-gluconate, 5-keto-D-gluconate
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, loss of activity within 1 week
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
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recombinant enzyme from Escherichia coli strain BL21(DE3)
to homogeneity, crystalline, chromatography techniques
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene 1029130, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
overexpression in Escherichia coli
Show AA Sequence (310 entries)
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