Information on EC 1.1.1.250 - D-arabinitol 2-dehydrogenase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.1.250
-
RECOMMENDED NAME
GeneOntology No.
D-arabinitol 2-dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-arabinitol + NAD+ = D-ribulose + NADH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
reduction
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
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Pentose and glucuronate interconversions
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-
SYSTEMATIC NAME
IUBMB Comments
D-arabinitol:NAD+ 2-oxidoreductase (D-ribulose-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
336883-93-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
gene tm0297
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-arabinitol + NAD+
D-ribulose + NADH + H+
show the reaction diagram
D-ribulose + NADH
D-arabinitol + NAD+
show the reaction diagram
D-xylitol + NAD+
D-xylulose + NADH + H+
show the reaction diagram
-
-
-
-
r
D-xylulose + NADH
?
show the reaction diagram
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1% of the activity with D-ribulose
-
-
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Galactitol + NAD+
?
show the reaction diagram
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1.6% of the activity with D-arabinitol
-
-
-
L-Xylulose + NADH
?
show the reaction diagram
-
3% of the activity with D-ribulose
-
-
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Xylitol + NAD+
?
show the reaction diagram
-
5% of the activity with D-arabinitol
-
-
-
additional information
?
-
-
the enzyme exclusively catalyzed the NAD(H)-dependent oxidoreduction of D-arabitol, D-xylitol, D-ribulose, or D-xylulose
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-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-ribulose + NADH
D-arabinitol + NAD+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
-
twofold activation at 60 mM, contributes to the overall stability of the protein and ise not required for catalytic activity
additional information
-
no effect on the activity by CaCl2 or NaCl
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.6 - 39.8
D-arabinitol
7.2 - 13.9
D-ribulose
20 - 21.7
D-xylitol
0.059 - 0.12
NAD+
0.027 - 0.031
NADH
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11 - 18.7
D-arabinitol
165.6 - 193.2
D-ribulose
11.2 - 16.6
D-xylitol
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.5 - 7.2
D-arabinitol
12 - 27
D-ribulose
0.56 - 0.74
D-xylitol
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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reduction reaction
8.5
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oxidation reaction
10 - 11
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oxidation of D-arabinitol
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 9.5
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40% and 10% of maximal activity at pH 5.5 and pH 8.5, no activity below pH 4.5 and above pH 9.5
8 - 11
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pH 8.0: about 40% of maximal activity, pH 8.5: about 60% of maximal activity, pH 10-11: maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50 - 85
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inactive below 50C, optimal activity at 85C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
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Thermotoga maritima cannot use D-arabitol as a sole carbon source. Optimal growth temperature of Thermotoga maritima is 80C
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
164000
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native PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 31000, SDS-PAGE
homohexamer
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6 * 27600, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11
-
unstable above
9566
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
85
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rapid inactivation of the recombinant Strep-tagged enzyme, while the detagged enzyme retains over 90% activity after 90 min at 85C
additional information
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the addition of the Strep-tag led to a destabilization of tmArDH resulting in the loss of thermostability
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Mg2+ enhances the stability
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80C, stable
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli and Saccharomyces cerevisiae
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gene tm0297, located in an operon with tm0298 encoding a mannitol dehydrogenase, phylogenetic analysis, expression of the Strep-tagged enzyme in Escherichia coli
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
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the specificity of the enzyme makes it useful for the development of a simple and specific method for the measurement of D-arabinitol, a metabolite of the pathogenic Candida spp. which has been described as a marker for disseminated candidiasis