Information on EC 1.1.1.23 - histidinol dehydrogenase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.1.23
-
RECOMMENDED NAME
GeneOntology No.
histidinol dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-histidinol + 2 NAD+ + H2O = L-histidine + 2 NADH + 3 H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
histidine metabolism
-
-
Histidine metabolism
-
-
Metabolic pathways
-
-
Biosynthesis of secondary metabolites
-
-
SYSTEMATIC NAME
IUBMB Comments
L-histidinol:NAD+ oxidoreductase
Also oxidizes L-histidinal. The Neurospora enzyme also catalyses the reactions of EC 3.5.4.19 (phosphoribosyl-AMP cyclohydrolase) and EC 3.6.1.31 (phosphoribosyl-ATP diphosphatase).
CAS REGISTRY NUMBER
COMMENTARY hide
9028-27-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
asparagus shoots
-
-
Manually annotated by BRENDA team
; obtained by transformation
-
-
Manually annotated by BRENDA team
obtained by transformation
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-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
spring cabbage
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
biovar 1
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
cucumber shoots
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-
Manually annotated by BRENDA team
lettuce shoots
-
-
Manually annotated by BRENDA team
rose cell culture
-
-
Manually annotated by BRENDA team
eggplant shoots
-
-
Manually annotated by BRENDA team
wheat germ
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-histidinal + NAD+
L-histidine + NADH
show the reaction diagram
L-histidinol + 2 NAD+ + H2O
L-histidine + 2 NADH + 3 H+
show the reaction diagram
L-histidinol + NAD+
L-histidinal + NADH + H+
show the reaction diagram
L-histidinol + NAD+
L-histidine + NADH
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-histidinal + NAD+
L-histidine + NADH
show the reaction diagram
L-histidinol + 2 NAD+ + H2O
L-histidine + 2 NADH + 3 H+
show the reaction diagram
L-histidinol + NAD+
L-histidinal + NADH + H+
show the reaction diagram
-
enzyme is responsible for catalysis of the terminal step in L-histidine biosynthesis, being performed in 2 sequential reactions via the intermediate L-histidinal
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-
ir
L-histidinol + NAD+
L-histidine + NADH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
I6Y6Z1
can partially substitute for Zn2+
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2S)-2-amino-3-(1H-imidazol-4-yl)-N'-(naphthalen-2-ylsulfonyl)propanehydrazide
-
-
(2S)-2-amino-3-(1H-imidazol-4-yl)-N'-[(4-methylphenyl)sulfonyl]propanehydrazide
-
-
(2S)-2-amino-N'-(biphenyl-4-ylsulfonyl)-3-(1H-imidazol-4-yl)propanehydrazide
(2S)-2-amino-N'-[(4-bromophenyl)sulfonyl]-3-(1H-imidazol-4-yl)propanehydrazide
-
-
(3S)-3-amino-1-(2,3,4,5,6-pentafluorophenyloxy)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
-
-
(3S)-3-amino-1-(2,3,4,5,6-pentafluorophenylthio)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
-
-
(3S)-3-amino-1-(2,4-difluorophenyl)-4-(1H-imidazol-4-yl)butan-2-one
-
-
(3S)-3-amino-1-(4-bromophenoxy)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
-
-
(3S)-3-amino-1-(4-bromophenyl)-4-(1H-imidazol-4-yl)butan-2-one
-
-
(3S)-3-amino-1-(4-bromophenylthio)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
-
-
(3S)-3-amino-1-(4-chlorophenyl)-4-(1H-imidazol-4-yl)butan-2-one
-
-
(3S)-3-amino-1-(4-fluorophenoxy)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
-
-
(3S)-3-amino-1-(4-fluorophenylthio)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
-
-
(3S)-3-amino-1-(4-hydroxyphenyl)-4-(1H-imidazol-4-yl)butan-2-one
-
(3S)-3-amino-1-([1,1'-biphenyl]-4-yl)-4-(1H-imidazol-4-yl)butan-2-one
-
-
(3S)-3-amino-1-hydroxy-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
-
-
(3S)-3-amino-1-thio-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
-
-
(3S)-3-amino-1-[4-(benzyloxy)phenyl]-4-(1H-imidazol-4-yl)butan-2-one
(3S)-3-amino-1-[4-(biphenyl-4-ylethynyl)phenyl]-4-(1H-imidazol-4-yl)butan-2-one
-
-
(3S)-3-amino-1-[4-(biphenyl-4-ylmethoxy)phenyl]-4-(1H-imidazol-4-yl)butan-2-one
-
-
(3S)-3-amino-1-[4-[(4-bromobenzyl)oxy]phenyl]-4-(1H-imidazol-4-yl)butan-2-one
-
-
(3S)-3-amino-1-[4-[(4-butylbenzyl)oxy]phenyl]-4-(1H-imidazol-4-yl)butan-2-one
-
50% inhibition of pathogen growth in minimal medium at 0.5 mM
(3S)-3-amino-1-[4-[(4-tert-butylphenyl)ethynyl]phenyl]-4-(1H-imidazol-4-yl)butan-2-one
-
30% inhibition of pathogen growth in minimal medium at 0.5 mM
(3S)-3-amino-1-[4-[(E)-2-(biphenyl-4-yl)ethenyl]phenyl]-4-(1H-imidazol-4-yl)butan-2-one
-
-
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(4-methylphenyl)butan-2-one
-
-
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(4-[(E)-2-[4-(trifluoromethyl)phenyl]ethenyl]phenyl)butan-2-one
-
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(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(4-[[4-(naphthalen-2-yl)benzyl]oxy]phenyl)butan-2-one
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complete inhibition of pathogen growth in minimal medium at 0.5 mM
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(4-[[4-(trifluoromethyl)benzyl]oxy]phenyl)butan-2-one
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70% inhibition of pathogen growth in minimal medium at 0.5 mM
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(naphthalen-2-yloxy)butan-2-one dihydrochloride
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(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(naphthalen-2-ylthio)butan-2-one dihydrochloride
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(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(p-tolyloxy)butan-2-one dihydrochloride
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(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(phenyloxy)butan-2-one dihydrochloride
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(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(phenylthio)butan-2-one dihydrochloride
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(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(tolylthio)butan-2-one dihydrochloride
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(3S)-3-amino-4-(1H-imidazol-4-yl)-1-phenylbutan-2-one
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(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-(2-phenylethyl)phenyl]butan-2-one
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(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-(7-phenylhept-1-yn-1-yl)phenyl]butan-2-one
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(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-(phenylethynyl)phenyl]butan-2-one
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complete inhibition of pathogen growth in minimal medium at 0.5 mM
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(1E)-3-phenylprop-1-en-1-yl]phenyl]butan-2-one
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-
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(4-methoxybenzyl)oxy]phenyl]butan-2-one
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30% inhibition of pathogen growth in minimal medium at 0.5 mM
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(4-methylbenzyl)oxy]phenyl]butan-2-one
-
30% inhibition of pathogen growth in minimal medium at 0.5 mM
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(4-methylphenyl)ethynyl]phenyl]butan-2-one
-
-
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(4-pentylphenyl)ethynyl]phenyl]butan-2-one
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30% inhibition of pathogen growth in minimal medium at 0.5 mM
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(4-phenoxybenzyl)oxy]phenyl]butan-2-one
-
70% inhibition of pathogen growth in minimal medium at 0.5 mM
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(E)-2-(4-methylphenyl)ethenyl]phenyl]butan-2-one
-
complete inhibition of pathogen growth in minimal medium at 0.5 mM
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(E)-2-phenylethenyl]phenyl]butan-2-one
-
-
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[4-(phenylethynyl)benzyl]phenyl]butan-2-one
(3S)-3-amino-4-(1H-imidazol-4-yl)butan-2-one
-
(7R)-1,3-diiodo-5-oxo-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylic acid
-
weaker inhibitor
(7S)-7-[(2,4-difluorophenyl)acetyl]-7,8-dihydroimidazo[1,5-c]pyrimidin-5(6H)-one
-
-
(7S)-7-[(4-bromophenyl)acetyl]-7,8-dihydroimidazo[1,5-c]pyrimidin-5(6H)-one
-
-
(7S)-7-[(4-chlorophenyl)acetyl]-7,8-dihydroimidazo[1,5-c]pyrimidin-5(6H)-one
-
-
(7S)-7-[(4-methylphenyl)acetyl]-7,8-dihydroimidazo[1,5-c]pyrimidin-5(6H)-one
-
-
(7S)-7-[([1,1'-biphenyl]-4-yl)acetyl]-7,8-dihydroimidazo[1,5-c]pyrimidin-5(6H)-one
-
-
(7S)-7-[[4-(7-phenylhept-1-yn-1-yl)phenyl]acetyl]-7,8-dihydroimidazo[1,5-c]pyrimidin-5(6H)-one
-
-
(8S)-8-[2-(biphenyl-4-yl)ethanethioyl]-7,8-dihydroimidazo[1,5-c]pyrimidine-5(6H)-thione
-
-
(S)-3-amino-1-(4-(benzyloxy)phenyl)-4-(1H-imidazol-4-yl)butan-2-one
-
-
(S)-3-amino-1-(4-bromophenyl)-4-(1H-imidazol-4-yl)butan-2-one
(S)-3-amino-1-(4-chlorophenyl)-4-(1H-imidazol-4-yl)butan-2-one
(S)-3-amino-1-(4-fluorophenyl)-4-(1H-imidazol-4-yl)butan-2-one
-
-
(S)-3-amino-4-(1H-imidazol-4-yl)-1-(4-phenoxyphenyl)butan-2-one
-
-
(S)-3-amino-4-(1H-imidazol-4-yl)-1-(naphthalen-2-yl)butan-2-one
(S)-3-amino-4-(1H-imidazol-4-yl)-1-p-tolylbutan-2-one
-
-
1,10-phenanthroline
I6Y6Z1
54% inhibition at 1 mM, 96% at 5 mM
1,3-diiodo-5-oxo-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylic acid
-
2-(2,4-difluorophenyl)-1-[(7S)-5-sulfanylidene-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidin-7-yl]ethan-1-one
-
-
2-(4-bromophenyl)-1-[(7S)-5-sulfanylidene-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidin-7-yl]ethan-1-one
-
-
2-(4-chlorophenyl)-1-[(7S)-5-sulfanylidene-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidin-7-yl]ethan-1-one
-
-
2-(4-methylphenyl)-1-[(7S)-5-sulfanylidene-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidin-7-yl]ethan-1-one
-
-
2-([1,1'-biphenyl]-4-yl)-1-[(7S)-5-sulfanylidene-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidin-7-yl]ethan-1-one
-
-
2-amino-3-(1H-imidazol-4-yl)-N'-[(E)-(4-methoxyphenyl)methylidene]propanehydrazide
-
-
2-amino-3-(1H-imidazol-4-yl)-N'-[(E)-(4-methylphenyl)methylidene]propanehydrazide
-
-
2-amino-3-(1H-imidazol-4-yl)-N'-[(E)-(4-nitrophenyl)methylidene]propanehydrazide
-
-
2-amino-3-(1H-imidazol-4-yl)-N'-[(E)-(4-phenoxyphenyl)methylidene]propanehydrazide
-
-
2-amino-3-(1H-imidazol-4-yl)-N'-[(E)-(naphthalen-2-yl)methylidene]propanehydrazide
-
-
2-amino-3-(1H-imidazol-4-yl)-N'-[(E)-phenylmethylidene]propanehydrazide (non-preferred name)
-
-
2-amino-N'-[(E)-(2-hydroxyphenyl)methylidene]-3-(1H-imidazol-4-yl)propanehydrazide
-
-
2-amino-N'-[(E)-(4-bromophenyl)methylidene]-3-(1H-imidazol-4-yl)propanehydrazide
-
-
2-amino-N'-[(E)-(4-chlorophenyl)methylidene]-3-(1H-imidazol-4-yl)propanehydrazide
-
-
2-amino-N'-[(E)-(4-fluorophenyl)methylidene]-3-(1H-imidazol-4-yl)propanehydrazide
-
-
2-amino-N'-[(E)-[4-(dimethylamino)phenyl]methylidene]-3-(1H-imidazol-4-yl)propanehydrazide
-
-
2-[4-(7-phenylhept-1-yn-1-yl)phenyl]-1-[(7S)-5-sulfanylidene-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidin-7-yl]ethan-1-one
-
-
3-(1H-imidazol-4-yl)propanoic acid
-
5-oxo-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylic acid
i.e. RJ-278
7,8-dihydroimidazo[1,5-c]pyrimidin-5(6H)-one
7-chloro-4-nitro-2,1,3-benzoxadiazole
-
0.1 mM, pseudo first order inactivation, binds to Cys-116 in active site
Ba2+
-
0.5 mM, 35% inhibition
Borate
-
10 mM, 90% inhibition
HgCl2
imidazole-4-carboxylic acid
-
most active inhibitor
methyl (7R)-1-chloro-5-oxo-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylate
-
weaker inhibitor
methyl (7S)-5-oxo-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylate
-
-
methyl (7S)-5-sulfanylidene-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylate
-
-
methyl 1-chloro-5-oxo-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylate
-
methyl 1-methyl-N-(phenoxycarbonyl)histidinate
-
methyl 3-(1H-imidazol-4-yl)propanoate
-
-
methyl N-(ethoxycarbonyl)-1-methyl-L-histidinate
-
-
methyl N-(methoxycarbonyl)-3-methyl-L-histidinate
-
-
methyl N-(methoxycarbonyl)histidinate
-
N-(4-dimethylamino-3,5-dinitrophenyl)-maleimide
Ni2+
-
0.5 mM, 54% inhibition
p-chloromercuribenzoate
pyridoxal 5'-phosphate
-
100 mM, 62.5% inhibition, concentration dependent, almost completely reversible
Urea
-
2.2 mM, 50% inhibition, reversible
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
I6Y6Z1
118% activity at 0.1 mM, 135% at 1 mM, slightly inhibitory above 10 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0027 - 0.0037
L-histidinal
0.0027 - 0.048
L-Histidinol
0.0027 - 1.4
NAD+
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.15 - 9.34
L-histidinal
1.1 - 35
L-Histidinol
0.227 - 1.361
NAD+
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
54950 - 71800
L-Histidinol
68.78 - 469
NAD+
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0032
2-amino-3-(1H-imidazol-4-yl)-N'-[(E)-(4-phenoxyphenyl)methylidene]propanehydrazide
-
pH 7.2, 25°C
0.00047
2-amino-3-(1H-imidazol-4-yl)-N'-[(E)-(naphthalen-2-yl)methylidene]propanehydrazide
-
pH 7.2, 25°C
0.00064
2-amino-N'-[(E)-(4-chlorophenyl)methylidene]-3-(1H-imidazol-4-yl)propanehydrazide
-
pH 7.2, 25°C
0.013
2-amino-N'-[(E)-(4-fluorophenyl)methylidene]-3-(1H-imidazol-4-yl)propanehydrazide
-
pH 7.2, 25°C
additional information
additional information
-
inhibition kinetics of hydrozone derivative inhibitors with enzyme MtHisD
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.14
(2S)-2-amino-3-(1H-imidazol-4-yl)-N'-(naphthalen-2-ylsulfonyl)propanehydrazide
Brucella suis
-
pH 9.2, 30°C
0.16
(2S)-2-amino-3-(1H-imidazol-4-yl)-N'-[(4-methylphenyl)sulfonyl]propanehydrazide
Brucella suis
-
pH 9.2, 30°C
0.025
(2S)-2-amino-N'-(biphenyl-4-ylsulfonyl)-3-(1H-imidazol-4-yl)propanehydrazide
0.07
(2S)-2-amino-N'-[(4-bromophenyl)sulfonyl]-3-(1H-imidazol-4-yl)propanehydrazide
Brucella suis
-
pH 9.2, 30°C
0.0028
(3S)-3-amino-1-(2,3,4,5,6-pentafluorophenyloxy)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30°C, recombinant His6-tagged enzyme
0.0192
(3S)-3-amino-1-(2,3,4,5,6-pentafluorophenylthio)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30°C, recombinant His6-tagged enzyme
0.01
(3S)-3-amino-1-(2,4-difluorophenyl)-4-(1H-imidazol-4-yl)butan-2-one
Brucella suis
-
at 30°C in the presence of 0.05 mM histidinol, 2 mM NAD+ and 0.5 mM MnCl2 in 150 mM sodium glycine buffer at pH 9.19
0.0016
(3S)-3-amino-1-(4-bromophenoxy)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30°C, recombinant His6-tagged enzyme
0.006
(3S)-3-amino-1-(4-bromophenyl)-4-(1H-imidazol-4-yl)butan-2-one
Brucella suis
-
at 30°C in the presence of 0.05 mM histidinol, 2 mM NAD+ and 0.5 mM MnCl2 in 150 mM sodium glycine buffer at pH 9.16
0.0028
(3S)-3-amino-1-(4-bromophenylthio)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30°C, recombinant His6-tagged enzyme
0.012
(3S)-3-amino-1-(4-chlorophenyl)-4-(1H-imidazol-4-yl)butan-2-one
Brucella suis
-
at 30°C in the presence of 0.05 mM histidinol, 2 mM NAD+ and 0.5 mM MnCl2 in 150 mM sodium glycine buffer at pH 9.17
0.0047
(3S)-3-amino-1-(4-fluorophenoxy)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30°C, recombinant His6-tagged enzyme
0.0031
(3S)-3-amino-1-(4-fluorophenylthio)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30°C, recombinant His6-tagged enzyme
0.00004
(3S)-3-amino-1-(4-hydroxyphenyl)-4-(1H-imidazol-4-yl)butan-2-one
Brassica oleracea
P24226
pH and temperature not specified in the publication
0.012
(3S)-3-amino-1-([1,1'-biphenyl]-4-yl)-4-(1H-imidazol-4-yl)butan-2-one
Brucella suis
-
at 30°C in the presence of 0.05 mM histidinol, 2 mM NAD+ and 0.5 mM MnCl2 in 150 mM sodium glycine buffer at pH 9.20
0.000024
(3S)-3-amino-1-hydroxy-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30°C, recombinant His6-tagged enzyme
0.0021
(3S)-3-amino-1-thio-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30°C, recombinant His6-tagged enzyme
0.000003 - 3
(3S)-3-amino-1-[4-(benzyloxy)phenyl]-4-(1H-imidazol-4-yl)butan-2-one
40
(3S)-3-amino-1-[4-[(4-butylbenzyl)oxy]phenyl]-4-(1H-imidazol-4-yl)butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
65
(3S)-3-amino-1-[4-[(4-tert-butylphenyl)ethynyl]phenyl]-4-(1H-imidazol-4-yl)butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
0.015
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(4-methylphenyl)butan-2-one
Brucella suis
-
at 30°C in the presence of 0.05 mM histidinol, 2 mM NAD+ and 0.5 mM MnCl2 in 150 mM sodium glycine buffer at pH 9.18
65
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(4-[(E)-2-[4-(trifluoromethyl)phenyl]ethenyl]phenyl)butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
30
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(4-[[4-(naphthalen-2-yl)benzyl]oxy]phenyl)butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
30
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(4-[[4-(trifluoromethyl)benzyl]oxy]phenyl)butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
0.00011
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(naphthalen-2-yloxy)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30°C, recombinant His6-tagged enzyme
0.0033
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(naphthalen-2-ylthio)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30°C, recombinant His6-tagged enzyme
0.00021
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(p-tolyloxy)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30°C, recombinant His6-tagged enzyme
0.0037
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(phenyloxy)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30°C, recombinant His6-tagged enzyme
0.0071
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(phenylthio)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30°C, recombinant His6-tagged enzyme
0.0395
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(tolylthio)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30°C, recombinant His6-tagged enzyme
0.001
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-phenylbutan-2-one
Escherichia coli
-
pH and temperature not specified in the publication
13
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-(2-phenylethyl)phenyl]butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
0.009
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-(7-phenylhept-1-yn-1-yl)phenyl]butan-2-one
Brucella suis
-
at 30°C in the presence of 0.05 mM histidinol, 2 mM NAD+ and 0.5 mM MnCl2 in 150 mM sodium glycine buffer at pH 9.21
3
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-(phenylethynyl)phenyl]butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
30
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(1E)-3-phenylprop-1-en-1-yl]phenyl]butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
20
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(4-methoxybenzyl)oxy]phenyl]butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
30
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(4-methylbenzyl)oxy]phenyl]butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
40
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(4-methylphenyl)ethynyl]phenyl]butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
8.5
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(4-pentylphenyl)ethynyl]phenyl]butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
70
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(4-phenoxybenzyl)oxy]phenyl]butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
40
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(E)-2-(4-methylphenyl)ethenyl]phenyl]butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
25
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(E)-2-phenylethenyl]phenyl]butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
0.000003 - 0.00007
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[4-(phenylethynyl)benzyl]phenyl]butan-2-one
0.005
(3S)-3-amino-4-(1H-imidazol-4-yl)butan-2-one
Salmonella enterica subsp. enterica serovar Typhimurium
P10370
pH and temperature not specified in the publication
0.0587
(7R)-1,3-diiodo-5-oxo-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylic acid
Geotrichum candidum
-
-
0.143
(7S)-7-[(2,4-difluorophenyl)acetyl]-7,8-dihydroimidazo[1,5-c]pyrimidin-5(6H)-one
Brucella suis
-
at 30°C in the presence of 0.05 mM histidinol, 2 mM NAD+ and 0.5 mM MnCl2 in 150 mM sodium glycine buffer at pH 9.6
0.098
(7S)-7-[(4-bromophenyl)acetyl]-7,8-dihydroimidazo[1,5-c]pyrimidin-5(6H)-one
Brucella suis
-
at 30°C in the presence of 0.05 mM histidinol, 2 mM NAD+ and 0.5 mM MnCl2 in 150 mM sodium glycine buffer at pH 9.3
0.092
(7S)-7-[(4-chlorophenyl)acetyl]-7,8-dihydroimidazo[1,5-c]pyrimidin-5(6H)-one
Brucella suis
-
at 30°C in the presence of 0.05 mM histidinol, 2 mM NAD+ and 0.5 mM MnCl2 in 150 mM sodium glycine buffer at pH 9.4
0.113
(7S)-7-[(4-methylphenyl)acetyl]-7,8-dihydroimidazo[1,5-c]pyrimidin-5(6H)-one
Brucella suis
-
at 30°C in the presence of 0.05 mM histidinol, 2 mM NAD+ and 0.5 mM MnCl2 in 150 mM sodium glycine buffer at pH 9.5
0.086
(7S)-7-[([1,1'-biphenyl]-4-yl)acetyl]-7,8-dihydroimidazo[1,5-c]pyrimidin-5(6H)-one
Brucella suis
-
at 30°C in the presence of 0.05 mM histidinol, 2 mM NAD+ and 0.5 mM MnCl2 in 150 mM sodium glycine buffer at pH 9.7
0.175
(7S)-7-[[4-(7-phenylhept-1-yn-1-yl)phenyl]acetyl]-7,8-dihydroimidazo[1,5-c]pyrimidin-5(6H)-one
Brucella suis
-
at 30°C in the presence of 0.05 mM histidinol, 2 mM NAD+ and 0.5 mM MnCl2 in 150 mM sodium glycine buffer at pH 9.8
0.005
(8S)-8-[2-(biphenyl-4-yl)ethanethioyl]-7,8-dihydroimidazo[1,5-c]pyrimidine-5(6H)-thione
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.000003
(S)-3-amino-1-(4-(benzyloxy)phenyl)-4-(1H-imidazol-4-yl)butan-2-one
Brucella suis
-
pH 9.2, 30°C
0.000006
(S)-3-amino-1-(4-bromophenyl)-4-(1H-imidazol-4-yl)butan-2-one
Brucella suis
-
pH 9.2, 30°C
0.000012
(S)-3-amino-1-(4-chlorophenyl)-4-(1H-imidazol-4-yl)butan-2-one
Brucella suis
-
pH 9.2, 30°C
0.000015
(S)-3-amino-1-(4-fluorophenyl)-4-(1H-imidazol-4-yl)butan-2-one
Brucella suis
-
pH 9.2, 30°C
0.000016
(S)-3-amino-4-(1H-imidazol-4-yl)-1-(4-phenoxyphenyl)butan-2-one
Brucella suis
-
pH 9.2, 30°C
0.0000145
(S)-3-amino-4-(1H-imidazol-4-yl)-1-(naphthalen-2-yl)butan-2-one
Brucella suis
-
pH 9.2, 30°C
0.000015
(S)-3-amino-4-(1H-imidazol-4-yl)-1-p-tolylbutan-2-one
Brucella suis
-
pH 9.2, 30°C
0.0587
1,3-diiodo-5-oxo-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylic acid
Geotrichum candidum
A1BPP9
pH not specified in the publication, temperature not specified in the publication
0.095
2-(2,4-difluorophenyl)-1-[(7S)-5-sulfanylidene-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidin-7-yl]ethan-1-one
Brucella suis
-
at 30°C in the presence of 0.05 mM histidinol, 2 mM NAD+ and 0.5 mM MnCl2 in 150 mM sodium glycine buffer at pH 9.13
0.105
2-(4-bromophenyl)-1-[(7S)-5-sulfanylidene-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidin-7-yl]ethan-1-one
Brucella suis
-
at 30°C in the presence of 0.05 mM histidinol, 2 mM NAD+ and 0.5 mM MnCl2 in 150 mM sodium glycine buffer at pH 9.10
0.074
2-(4-chlorophenyl)-1-[(7S)-5-sulfanylidene-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidin-7-yl]ethan-1-one
Brucella suis
-
at 30°C in the presence of 0.05 mM histidinol, 2 mM NAD+ and 0.5 mM MnCl2 in 150 mM sodium glycine buffer at pH 9.11
0.095
2-(4-methylphenyl)-1-[(7S)-5-sulfanylidene-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidin-7-yl]ethan-1-one
Brucella suis
-
at 30°C in the presence of 0.05 mM histidinol, 2 mM NAD+ and 0.5 mM MnCl2 in 150 mM sodium glycine buffer at pH 9.12
0.005
2-([1,1'-biphenyl]-4-yl)-1-[(7S)-5-sulfanylidene-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidin-7-yl]ethan-1-one
Brucella suis
-
at 30°C in the presence of 0.05 mM histidinol, 2 mM NAD+ and 0.5 mM MnCl2 in 150 mM sodium glycine buffer at pH 9.14
0.005
2-amino-3-(1H-imidazol-4-yl)-N'-[(E)-(4-methoxyphenyl)methylidene]propanehydrazide
Mycobacterium tuberculosis
-
pH 7.2, 25°C
0.0055
2-amino-3-(1H-imidazol-4-yl)-N'-[(E)-(4-methylphenyl)methylidene]propanehydrazide
Mycobacterium tuberculosis
-
pH 7.2, 25°C
0.0025
2-amino-3-(1H-imidazol-4-yl)-N'-[(E)-(4-nitrophenyl)methylidene]propanehydrazide
Mycobacterium tuberculosis
-
pH 7.2, 25°C
0.0098
2-amino-3-(1H-imidazol-4-yl)-N'-[(E)-(4-phenoxyphenyl)methylidene]propanehydrazide
Mycobacterium tuberculosis
-
pH 7.2, 25°C
0.0011
2-amino-3-(1H-imidazol-4-yl)-N'-[(E)-(naphthalen-2-yl)methylidene]propanehydrazide
Mycobacterium tuberculosis
-
pH 7.2, 25°C
0.029
2-amino-3-(1H-imidazol-4-yl)-N'-[(E)-phenylmethylidene]propanehydrazide (non-preferred name)
Mycobacterium tuberculosis
-
pH 7.2, 25°C
0.027
2-amino-N'-[(E)-(2-hydroxyphenyl)methylidene]-3-(1H-imidazol-4-yl)propanehydrazide
Mycobacterium tuberculosis
-
pH 7.2, 25°C
0.0077
2-amino-N'-[(E)-(4-bromophenyl)methylidene]-3-(1H-imidazol-4-yl)propanehydrazide
Mycobacterium tuberculosis
-
pH 7.2, 25°C
0.0024
2-amino-N'-[(E)-(4-chlorophenyl)methylidene]-3-(1H-imidazol-4-yl)propanehydrazide
Mycobacterium tuberculosis
-
pH 7.2, 25°C
0.0024
2-amino-N'-[(E)-(4-fluorophenyl)methylidene]-3-(1H-imidazol-4-yl)propanehydrazide
Mycobacterium tuberculosis
-
pH 7.2, 25°C
0.0091
2-amino-N'-[(E)-[4-(dimethylamino)phenyl]methylidene]-3-(1H-imidazol-4-yl)propanehydrazide
Mycobacterium tuberculosis
-
pH 7.2, 25°C
0.322
2-[4-(7-phenylhept-1-yn-1-yl)phenyl]-1-[(7S)-5-sulfanylidene-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidin-7-yl]ethan-1-one
Brucella suis
-
at 30°C in the presence of 0.05 mM histidinol, 2 mM NAD+ and 0.5 mM MnCl2 in 150 mM sodium glycine buffer at pH 9.15
0.00317
3-(1H-imidazol-4-yl)propanoic acid
Geotrichum candidum
A1BPP9
pH not specified in the publication, temperature not specified in the publication
0.00356
5-oxo-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylic acid
Geotrichum candidum
A1BPP9
pH not specified in the publication, temperature not specified in the publication
0.0242
7,8-dihydroimidazo[1,5-c]pyrimidin-5(6H)-one
0.0064
imidazole-4-carboxylic acid
Geotrichum candidum
-
-
0.1222
methyl (7R)-1-chloro-5-oxo-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylate
Geotrichum candidum
-
-
0.532
methyl (7S)-5-oxo-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylate
Brucella suis
-
at 30°C in the presence of 0.05 mM histidinol, 2 mM NAD+ and 0.5 mM MnCl2 in 150 mM sodium glycine buffer at pH 9.2
0.018
methyl (7S)-5-sulfanylidene-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylate
Brucella suis
-
at 30°C in the presence of 0.05 mM histidinol, 2 mM NAD+ and 0.5 mM MnCl2 in 150 mM sodium glycine buffer at pH 9.9
0.1222
methyl 1-chloro-5-oxo-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylate
Geotrichum candidum
A1BPP9
pH not specified in the publication, temperature not specified in the publication
0.0293
methyl 1-methyl-N-(phenoxycarbonyl)histidinate
Geotrichum candidum
A1BPP9
pH not specified in the publication, temperature not specified in the publication
0.0052
methyl 3-(1H-imidazol-4-yl)propanoate
0.0293
methyl N-(ethoxycarbonyl)-1-methyl-L-histidinate
Geotrichum candidum
-
-
0.0106
methyl N-(methoxycarbonyl)-3-methyl-L-histidinate
Geotrichum candidum
-
-
0.0106
methyl N-(methoxycarbonyl)histidinate
Geotrichum candidum
A1BPP9
pH not specified in the publication, temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0003
-
enriched extracts
0.0004
0.0009
-
enriched extracts
0.0016
-
enriched extracts
0.0021
-
enriched extracts
0.0061
-
enriched extracts
0.0062
-
enriched extracts
1.2
I6Y6Z1
purified recombinant enzyme, pH 7.2, 25°C
10
-
recombinant enzym
additional information
-
the activity of the enzyme in different tissues at different pH values, development of a determination method for quantification of L-histidinol and L-histidine using isocratic HPLC, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2 - 9
-
assay at
7.2
I6Y6Z1
assay at
8.6
-
crude extracts
8.7
-
partly purified enzyme
additional information
-
the activity of the enzyme in different tissues is differently regulated with respect to the pH value, overview
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 12
-
-
additional information
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 40
-
assay at
30
-
assay at
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
-
highest activity
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
-
very low activity
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
additional information
-
activity in the organs in descending order: liver, kidney, pancreas, spleen, skeletal muscle, small intestine
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43000
-
alpha,alpha, 2 * 43000, SDS-PAGE
45348
I6Y6Z1
2 * 45378, sequence calculation, 2 * 45348, recombinant enzyme, mass spectrometry
45378
I6Y6Z1
2 * 45378, sequence calculation, 2 * 45348, recombinant enzyme, mass spectrometry
91000
-
gel filtration
100000
-
recombinant enzyme expressed in SF9 cells, native PAGE
101800
I6Y6Z1
gel filtration, recombinant enzyme
103000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant detagged enzyme mutant C366S, in apoform or bound to inhibitor (3S)-3-amino-1-[4-(benzyloxy)phenyl]-4-(1H-imidazol-4-yl)butan-2-one, hanging drop vapor diffusion method, protein solution containing 50 mM Tris-HCl, 150 mM NaCl, and 1 mM DTT, as well as 1 mM ZnCl2 and 20 mM inhibitor 1, is mixed with reservoir solution containing 25% PEG 3350, 0.1 M HEPES pH 8, 0.25 M NaCl, 20°C, equilibration against 1 ml of reservoir solution, method optimzation, X-ray diffraction structure determination and analysis at 1.90 A resolution, molecular replacement and modelling
15.4 mg/ml purified recombinant detagged enzyme alone or complexed with L-histidinol, L-histamine, or L-histidine or Zn2+ and NAD+, in 20 mM Tris-HCl, pH 7.5, 0.2 M NaCl, 5 mM DTT, 1 mM ligand, hanging drop vapour diffusion method, 18°C, 0.002 ml protein solution with 0.004 ml reservoir solution, containing 20% w/v PEG 3350, 7% v/v glycerol, 0.1 M imidazole-malic acid, pH 5.5, 0.2 M ammonium sulfate, 2 weeks, macroseeding for larger crystals, transfer to sodium acetate, pH 5.5, to eliminate the inhibiting imidazole, X-ray diffraction structure determination and analysis at 2.2 A resolution, modelling
-
crystal structure determination of HDH in its native state and with several substrates and Zn2+. the NAD+ molecule is crystallized with L-histidinol into the active site. In the apo structure, the Zn2+ coordination is tetrahedral, while it is octahedral in the inhibitor/enzyme complex
-
only a C366S mutant allows crystallization to proceed, probably forbidding oxidation/reduction of the native enzyme at this position, molecular replacement using the structure of the Escherichia coli enzyme. In the apo structure, the Zn2+ coordination is tetrahedral, while it is octahedral in the inhibitor/enzyme complex
-
1.6-2.0 mM ammonium sulfate solution at 4°C, pH 6.5, space group C2, monoclinic
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.2 - 10
-
rapid loss of activity above pH 10
286329
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65
-
almost no loss of activity after 5 min at pH 7.5, histidinol, 2 mM and MnCl2, 1 mM, protect enzyme from heat inactivation
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C, 0.05 M Tris-maleate, pH 6.5, 0.1 mM MnCl2, 3 months, 45%
-
4°C, 1 week, 10%
-
4°C, 20% glycerol, 1 week, 0%
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
55°C, 40% ammonium sulfate, Sephadex G-50, DEAE-cellulose, crystallization
60°C, pH 5 treatment, ammonium sulfate precipitation, gel filtration, DEAE-cellulose, gel filtration
-
ammonium sulfate precipitation, DEAE-chromatography, L-histidinol-Sepharose 4B affinity chromatography
-
ammonium sulfate precipitation, heating, affinity chromatography, preparative gel
-
enzyme expressed in SF9 cells, cell homogenate, Mono Q FPLC
-
heating, pH 4.8, ammonium sulfate, DEAE-cellulose
-
recombinant enzyme by anion exchange chromatography and gel filtration
-
recombinant enzyme from Escherichia coli strain BL21(DE3) 40fold to homogeneity by anion exchange chromatography, ultrafiltration, gel filtration, and another step of anion exchange chromatography
I6Y6Z1
recombinant enzyme fusion protein
-
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by glutathione affinity chromatography, tag cleavage, and gel filtration, and recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and gel filtration
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography
-
recombinant protein
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Spodoptera frugiperda, SF9 cells
expression in Escherichia coli
-
gene hisD, expression as glutathione S-transferase fusion protein with a thrombin cleavage site between tag and enzyme
-
gene hisD, overexpression in Escherichia coli strain BL21(DE3)
I6Y6Z1
gene hisD, overexpression of His-tagged HDH in Escherichia coli
gene hisD, recombinant overexpression
-
recombinant expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS, recombinant expressiom of His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
recombinant expression of the His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C112A
-
conserved Cys-112 is not involved in catalysis, Cys-112 corresponds to Cys-116 in Salmonella typhimurium
H261N
-
mutation causes complete loss of Zn and activity
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
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