Information on EC 1.1.1.23 - histidinol dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.1.1.23
-
RECOMMENDED NAME
GeneOntology No.
histidinol dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-histidinol + 2 NAD+ + H2O = L-histidine + 2 NADH + 3 H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
-
histidine metabolism
-
-
Histidine metabolism
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
L-histidinol:NAD+ oxidoreductase
Also oxidizes L-histidinal. The Neurospora enzyme also catalyses the reactions of EC 3.5.4.19 (phosphoribosyl-AMP cyclohydrolase) and EC 3.6.1.31 (phosphoribosyl-ATP diphosphatase).
CAS REGISTRY NUMBER
COMMENTARY hide
9028-27-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Arthrobacter histidinolovorans
-
-
-
Manually annotated by BRENDA team
asparagus shoots
-
-
Manually annotated by BRENDA team
; obtained by transformation
-
-
Manually annotated by BRENDA team
obtained by transformation
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
spring cabbage
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
cucumber shoots
-
-
Manually annotated by BRENDA team
lettuce shoots
-
-
Manually annotated by BRENDA team
gene hisD
I6Y6Z1
UniProt
Manually annotated by BRENDA team
gene hisD
I6Y6Z1
UniProt
Manually annotated by BRENDA team
rose cell culture
-
-
Manually annotated by BRENDA team
eggplant shoots
-
-
Manually annotated by BRENDA team
wheat germ
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
enzyme inhibition leads to inhibition of intracellular bacterial growth in macrophage infection of the facultative intracellular pathogen
metabolism
physiological function
-
the enzyme is essential for intramacrophagic replication
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-histidinal + NAD+
L-histidine + NADH
show the reaction diagram
L-histidinol + 2 NAD+ + H2O
L-histidine + 2 NADH + 3 H+
show the reaction diagram
L-histidinol + NAD+
L-histidinal + NADH + H+
show the reaction diagram
L-histidinol + NAD+
L-histidine + NADH
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-histidinal + NAD+
L-histidine + NADH
show the reaction diagram
L-histidinol + 2 NAD+ + H2O
L-histidine + 2 NADH + 3 H+
show the reaction diagram
L-histidinol + NAD+
L-histidinal + NADH + H+
show the reaction diagram
-
enzyme is responsible for catalysis of the terminal step in L-histidine biosynthesis, being performed in 2 sequential reactions via the intermediate L-histidinal
-
-
ir
L-histidinol + NAD+
L-histidine + NADH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
I6Y6Z1
can partially substitute for Zn2+
Cd2+
-
0.02-0.2 mM, 75% increase
Mg2+
I6Y6Z1
can partially substitute for Zn2+
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2S)-2-amino-3-(1H-imidazol-4-yl)-N'-(naphthalen-2-ylsulfonyl)propanehydrazide
-
-
(2S)-2-amino-3-(1H-imidazol-4-yl)-N'-[(4-methylphenyl)sulfonyl]propanehydrazide
-
-
(2S)-2-amino-N'-(biphenyl-4-ylsulfonyl)-3-(1H-imidazol-4-yl)propanehydrazide
-
-
(2S)-2-amino-N'-[(4-bromophenyl)sulfonyl]-3-(1H-imidazol-4-yl)propanehydrazide
-
-
(3S)-3-amino-1-(2,3,4,5,6-pentafluorophenyloxy)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
-
-
(3S)-3-amino-1-(2,3,4,5,6-pentafluorophenylthio)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
-
-
(3S)-3-amino-1-(4-bromophenoxy)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
-
-
(3S)-3-amino-1-(4-bromophenylthio)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
-
-
(3S)-3-amino-1-(4-fluorophenoxy)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
-
-
(3S)-3-amino-1-(4-fluorophenylthio)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
-
-
(3S)-3-amino-1-hydroxy-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
-
-
(3S)-3-amino-1-thio-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
-
-
(3S)-3-amino-1-[4-(benzyloxy)phenyl]-4-(1H-imidazol-4-yl)butan-2-one
-
-
(3S)-3-amino-1-[4-(biphenyl-4-ylethynyl)phenyl]-4-(1H-imidazol-4-yl)butan-2-one
-
-
(3S)-3-amino-1-[4-(biphenyl-4-ylmethoxy)phenyl]-4-(1H-imidazol-4-yl)butan-2-one
-
-
(3S)-3-amino-1-[4-[(4-bromobenzyl)oxy]phenyl]-4-(1H-imidazol-4-yl)butan-2-one
-
-
(3S)-3-amino-1-[4-[(4-butylbenzyl)oxy]phenyl]-4-(1H-imidazol-4-yl)butan-2-one
-
50% inhibition of pathogen growth in minimal medium at 0.5 mM
(3S)-3-amino-1-[4-[(4-tert-butylphenyl)ethynyl]phenyl]-4-(1H-imidazol-4-yl)butan-2-one
-
30% inhibition of pathogen growth in minimal medium at 0.5 mM
(3S)-3-amino-1-[4-[(E)-2-(biphenyl-4-yl)ethenyl]phenyl]-4-(1H-imidazol-4-yl)butan-2-one
-
-
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(4-[(E)-2-[4-(trifluoromethyl)phenyl]ethenyl]phenyl)butan-2-one
-
-
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(4-[[4-(naphthalen-2-yl)benzyl]oxy]phenyl)butan-2-one
-
complete inhibition of pathogen growth in minimal medium at 0.5 mM
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(4-[[4-(trifluoromethyl)benzyl]oxy]phenyl)butan-2-one
-
70% inhibition of pathogen growth in minimal medium at 0.5 mM
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(naphthalen-2-yloxy)butan-2-one dihydrochloride
-
-
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(naphthalen-2-ylthio)butan-2-one dihydrochloride
-
-
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(p-tolyloxy)butan-2-one dihydrochloride
-
-
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(phenyloxy)butan-2-one dihydrochloride
-
-
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(phenylthio)butan-2-one dihydrochloride
-
-
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(tolylthio)butan-2-one dihydrochloride
-
-
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-(2-phenylethyl)phenyl]butan-2-one
-
-
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-(phenylethynyl)phenyl]butan-2-one
-
complete inhibition of pathogen growth in minimal medium at 0.5 mM
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(1E)-3-phenylprop-1-en-1-yl]phenyl]butan-2-one
-
-
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(4-methoxybenzyl)oxy]phenyl]butan-2-one
-
30% inhibition of pathogen growth in minimal medium at 0.5 mM
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(4-methylbenzyl)oxy]phenyl]butan-2-one
-
30% inhibition of pathogen growth in minimal medium at 0.5 mM
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(4-methylphenyl)ethynyl]phenyl]butan-2-one
-
-
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(4-pentylphenyl)ethynyl]phenyl]butan-2-one
-
30% inhibition of pathogen growth in minimal medium at 0.5 mM
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(4-phenoxybenzyl)oxy]phenyl]butan-2-one
-
70% inhibition of pathogen growth in minimal medium at 0.5 mM
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(E)-2-(4-methylphenyl)ethenyl]phenyl]butan-2-one
-
complete inhibition of pathogen growth in minimal medium at 0.5 mM
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(E)-2-phenylethenyl]phenyl]butan-2-one
-
-
(7R)-1,3-diiodo-5-oxo-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylic acid
-
weaker inhibitor
(S)-3-amino-1-(4-(benzyloxy)phenyl)-4-(1H-imidazol-4-yl)butan-2-one
-
-
(S)-3-amino-1-(4-bromophenyl)-4-(1H-imidazol-4-yl)butan-2-one
(S)-3-amino-1-(4-chlorophenyl)-4-(1H-imidazol-4-yl)butan-2-one
(S)-3-amino-1-(4-fluorophenyl)-4-(1H-imidazol-4-yl)butan-2-one
-
-
(S)-3-amino-4-(1H-imidazol-4-yl)-1-(4-phenoxyphenyl)butan-2-one
-
-
(S)-3-amino-4-(1H-imidazol-4-yl)-1-(naphthalen-2-yl)butan-2-one
(S)-3-amino-4-(1H-imidazol-4-yl)-1-p-tolylbutan-2-one
-
-
1,10-phenanthroline
I6Y6Z1
54% inhibition at 1 mM, 96% at 5 mM
1,3-diiodo-5-oxo-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylic acid
-
3-(1H-imidazol-4-yl)propanoic acid
-
5-oxo-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylic acid
i.e. RJ-278
7,8-dihydroimidazo[1,5-c]pyrimidin-5(6H)-one
7-chloro-4-nitro-2,1,3-benzoxadiazole
-
0.1 mM, pseudo first order inactivation, binds to Cys-116 in active site
Ba2+
-
0.5 mM, 35% inhibition
Borate
Arthrobacter histidinolovorans
-
10 mM, 90% inhibition
imidazole-4-carboxylic acid
-
most active inhibitor
methyl (7R)-1-chloro-5-oxo-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylate
-
weaker inhibitor
methyl 1-chloro-5-oxo-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylate
-
methyl 1-methyl-N-(phenoxycarbonyl)histidinate
-
methyl 3-(1H-imidazol-4-yl)propanoate
-
-
methyl N-(ethoxycarbonyl)-1-methyl-L-histidinate
-
-
methyl N-(methoxycarbonyl)-3-methyl-L-histidinate
-
-
methyl N-(methoxycarbonyl)histidinate
-
N-(4-dimethylamino-3,5-dinitrophenyl)-maleimide
Ni2+
-
0.5 mM, 54% inhibition
p-chloromercuribenzoate
pyridoxal 5'-phosphate
-
100 mM, 62.5% inhibition, concentration dependent, almost completely reversible
Urea
-
2.2 mM, 50% inhibition, reversible
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
I6Y6Z1
118% activity at 0.1 mM, 135% at 1 mM, slightly inhibitory above 10 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0027 - 0.0037
L-histidinal
0.0049 - 0.048
L-Histidinol
0.0072 - 1.4
NAD+
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.15 - 9.34
L-histidinal
1.1 - 35
L-Histidinol
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.14
(2S)-2-amino-3-(1H-imidazol-4-yl)-N'-(naphthalen-2-ylsulfonyl)propanehydrazide
Brucella suis
-
pH 9.2, 30C
0.16
(2S)-2-amino-3-(1H-imidazol-4-yl)-N'-[(4-methylphenyl)sulfonyl]propanehydrazide
Brucella suis
-
pH 9.2, 30C
0.025
(2S)-2-amino-N'-(biphenyl-4-ylsulfonyl)-3-(1H-imidazol-4-yl)propanehydrazide
Brucella suis
-
pH 9.2, 30C
0.07
(2S)-2-amino-N'-[(4-bromophenyl)sulfonyl]-3-(1H-imidazol-4-yl)propanehydrazide
Brucella suis
-
pH 9.2, 30C
0.0028
(3S)-3-amino-1-(2,3,4,5,6-pentafluorophenyloxy)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30C, recombinant His6-tagged enzyme
0.0192
(3S)-3-amino-1-(2,3,4,5,6-pentafluorophenylthio)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30C, recombinant His6-tagged enzyme
0.0016
(3S)-3-amino-1-(4-bromophenoxy)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30C, recombinant His6-tagged enzyme
0.0028
(3S)-3-amino-1-(4-bromophenylthio)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30C, recombinant His6-tagged enzyme
0.0047
(3S)-3-amino-1-(4-fluorophenoxy)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30C, recombinant His6-tagged enzyme
0.0031
(3S)-3-amino-1-(4-fluorophenylthio)-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30C, recombinant His6-tagged enzyme
0.000024
(3S)-3-amino-1-hydroxy-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30C, recombinant His6-tagged enzyme
0.0021
(3S)-3-amino-1-thio-4-(1H-imidazol-4-yl)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30C, recombinant His6-tagged enzyme
3
(3S)-3-amino-1-[4-(benzyloxy)phenyl]-4-(1H-imidazol-4-yl)butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
40
(3S)-3-amino-1-[4-[(4-butylbenzyl)oxy]phenyl]-4-(1H-imidazol-4-yl)butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
65
(3S)-3-amino-1-[4-[(4-tert-butylphenyl)ethynyl]phenyl]-4-(1H-imidazol-4-yl)butan-2-one
30
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(4-[[4-(naphthalen-2-yl)benzyl]oxy]phenyl)butan-2-one
0.00011
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(naphthalen-2-yloxy)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30C, recombinant His6-tagged enzyme
0.0033
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(naphthalen-2-ylthio)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30C, recombinant His6-tagged enzyme
0.00021
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(p-tolyloxy)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30C, recombinant His6-tagged enzyme
0.0037
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(phenyloxy)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30C, recombinant His6-tagged enzyme
0.0071
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(phenylthio)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30C, recombinant His6-tagged enzyme
0.0395
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-(tolylthio)butan-2-one dihydrochloride
Brucella sp.
-
pH 9.2, 30C, recombinant His6-tagged enzyme
13
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-(2-phenylethyl)phenyl]butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
3
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-(phenylethynyl)phenyl]butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
30
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(1E)-3-phenylprop-1-en-1-yl]phenyl]butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
20
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(4-methoxybenzyl)oxy]phenyl]butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
30
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(4-methylbenzyl)oxy]phenyl]butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
40
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(4-methylphenyl)ethynyl]phenyl]butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
8.5
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(4-pentylphenyl)ethynyl]phenyl]butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
70
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(4-phenoxybenzyl)oxy]phenyl]butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
40
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(E)-2-(4-methylphenyl)ethenyl]phenyl]butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
25
(3S)-3-amino-4-(1H-imidazol-4-yl)-1-[4-[(E)-2-phenylethenyl]phenyl]butan-2-one
Brucella suis
-
pH and temperature not specified in the publication
0.0587
(7R)-1,3-diiodo-5-oxo-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylic acid
Geotrichum candidum
-
-
0.000003
(S)-3-amino-1-(4-(benzyloxy)phenyl)-4-(1H-imidazol-4-yl)butan-2-one
Brucella suis
-
pH 9.2, 30C
0.000006
(S)-3-amino-1-(4-bromophenyl)-4-(1H-imidazol-4-yl)butan-2-one
Brucella suis
-
pH 9.2, 30C
0.000012
(S)-3-amino-1-(4-chlorophenyl)-4-(1H-imidazol-4-yl)butan-2-one
Brucella suis
-
pH 9.2, 30C
0.000015
(S)-3-amino-1-(4-fluorophenyl)-4-(1H-imidazol-4-yl)butan-2-one
Brucella suis
-
pH 9.2, 30C
0.000016
(S)-3-amino-4-(1H-imidazol-4-yl)-1-(4-phenoxyphenyl)butan-2-one
Brucella suis
-
pH 9.2, 30C
0.0000145
(S)-3-amino-4-(1H-imidazol-4-yl)-1-(naphthalen-2-yl)butan-2-one
Brucella suis
-
pH 9.2, 30C
0.000015
(S)-3-amino-4-(1H-imidazol-4-yl)-1-p-tolylbutan-2-one
Brucella suis
-
pH 9.2, 30C
0.0587
1,3-diiodo-5-oxo-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylic acid
Geotrichum candidum
A1BPP9
pH not specified in the publication, temperature not specified in the publication
0.00317
3-(1H-imidazol-4-yl)propanoic acid
Geotrichum candidum
A1BPP9
pH not specified in the publication, temperature not specified in the publication
0.00356
5-oxo-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylic acid
Geotrichum candidum
A1BPP9
pH not specified in the publication, temperature not specified in the publication
0.0242
7,8-dihydroimidazo[1,5-c]pyrimidin-5(6H)-one
0.0064
imidazole-4-carboxylic acid
Geotrichum candidum
-
-
0.1222
methyl (7R)-1-chloro-5-oxo-5,6,7,8-tetrahydroimidazo[1,5-c]pyrimidine-7-carboxylate
0.0293
methyl 1-methyl-N-(phenoxycarbonyl)histidinate
Geotrichum candidum
A1BPP9
pH not specified in the publication, temperature not specified in the publication
0.0052
methyl 3-(1H-imidazol-4-yl)propanoate
0.0293
methyl N-(ethoxycarbonyl)-1-methyl-L-histidinate
Geotrichum candidum
-
-
0.0106
methyl N-(methoxycarbonyl)-3-methyl-L-histidinate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0003
-
enriched extracts
0.0004
0.0009
-
enriched extracts
0.0016
-
enriched extracts
0.0021
-
enriched extracts
0.0061
-
enriched extracts
0.0062
-
enriched extracts
1.2
I6Y6Z1
purified recombinant enzyme, pH 7.2, 25C
10
-
recombinant enzym
additional information
-
the activity of the enzyme in different tissues at different pH values, development of a determination method for quantification of L-histidinol and L-histidine using isocratic HPLC, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2
I6Y6Z1
assay at
8.6
-
crude extracts
8.7
Arthrobacter histidinolovorans
-
partly purified enzyme
additional information
-
the activity of the enzyme in different tissues is differently regulated with respect to the pH value, overview
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 12
-
-
additional information
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
-
highest activity
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
-
very low activity
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
additional information
-
activity in the organs in descending order: liver, kidney, pancreas, spleen, skeletal muscle, small intestine
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Brucella suis biovar 1 (strain 1330)
Brucella suis biovar 1 (strain 1330)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
91000
-
gel filtration
100000
-
recombinant enzyme expressed in SF9 cells, native PAGE
101800
I6Y6Z1
gel filtration, recombinant enzyme
103000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
15.4 mg/ml purified recombinant detagged enzyme alone or complexed with L-histidinol, L-histamine, or L-histidine or Zn2+ and NAD+, in 20 mM Tris-HCl, pH 7.5, 0.2 M NaCl, 5 mM DTT, 1 mM ligand, hanging drop vapour diffusion method, 18C, 0.002 ml protein solution with 0.004 ml reservoir solution, containing 20% w/v PEG 3350, 7% v/v glycerol, 0.1 M imidazole-malic acid, pH 5.5, 0.2 M ammonium sulfate, 2 weeks, macroseeding for larger crystals, transfer to sodium acetate, pH 5.5, to eliminate the inhibiting imidazole, X-ray diffraction structure determination and analysis at 2.2 A resolution, modelling
1.6-2.0 mM ammonium sulfate solution at 4C, pH 6.5, space group C2, monoclinic
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.2 - 10
-
rapid loss of activity above pH 10
286329
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65
-
almost no loss of activity after 5 min at pH 7.5, histidinol, 2 mM and MnCl2, 1 mM, protect enzyme from heat inactivation
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0C, 0.05 M Tris-maleate, pH 6.5, 0.1 mM MnCl2, 3 months, 45%
-
4C, 1 week, 10%
-
4C, 20% glycerol, 1 week, 0%
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
55C, 40% ammonium sulfate, Sephadex G-50, DEAE-cellulose, crystallization
60C, pH 5 treatment, ammonium sulfate precipitation, gel filtration, DEAE-cellulose, gel filtration
-
ammonium sulfate precipitation, DEAE-chromatography, L-histidinol-Sepharose 4B affinity chromatography
-
ammonium sulfate precipitation, heating, affinity chromatography, preparative gel
-
enzyme expressed in SF9 cells, cell homogenate, Mono Q FPLC
-
heating, pH 4.8, ammonium sulfate, DEAE-cellulose
-
recombinant enzyme from Escherichia coli strain BL21(DE3) 40fold to homogeneity by anion exchange chromatography, ultrafiltration, gel filtration, and another step of anion exchange chromatography
I6Y6Z1
recombinant enzyme fusion protein
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography
-
recombinant protein
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Spodoptera frugiperda, SF9 cells
expression in Escherichia coli
-
gene hisD, expression as glutathione S-transferase fusion protein with a thrombin cleavage site between tag and enzyme
gene hisD, overexpression in Escherichia coli strain BL21(DE3)
I6Y6Z1
recombinant expression of the His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C112A
-
conserved Cys-112 is not involved in catalysis, Cys-112 corresponds to Cys-116 in Salmonella typhimurium
H261N
-
mutation causes complete loss of Zn and activity
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
-
histidinol dehydrogenase is a suitable target for not only for Geotrichum candidum but also for related species, wherby compounds identified can serve as a valuable lead for development of novel non-classical antifungal therapy, notably against strain like Candida rapidly becoming resistant to conventional therapy
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