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Information on EC 1.1.1.189 - prostaglandin-E2 9-reductase and Organism(s) Sus scrofa and UniProt Accession Q28960
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1.1.1.189 prostaglandin-E2 9-reductaseIUBMB Comments
Reduces prostaglandin E2 to prostaglandin F2alpha. A number of other 9-oxo- and 15-oxo-prostaglandin derivatives can also be reduced to the corresponding hydroxy compounds. May be identical with EC 1.1.1.197 15-hydroxyprostaglandin dehydrogenase (NADP+).
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Word Map
- 1.1.1.189
-
pgf2alpha
- luteal
- indomethacin
-
corpora
-
pseudopregnancy
-
luteolysis
-
13,14-dihydro-15-keto
-
cyclooxygenases
- furosemide
- medicine
The taxonomic range for the selected organisms is: Sus scrofa
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Synonyms
pge2-9-ketoreductase, pge2-9-k, pge 9-ketoreductase, prostaglandin e2 9-ketoreductase, pge2 9-ketoreductase, 9-kpr, prostaglandin-e2 9-reductase, pge2-9-or, prostaglandin 9-ketoreductase/carbonyl reductase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
20-alpha-HSD
-
-
-
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20-alpha-hydroxysteroid dehydrogenase
-
-
-
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9-keto-prostaglandin E2 reductase
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-
-
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9-ketoprostaglandin reductase
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-
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PGE2 9-ketoreductase
PGE2-9-ketoreductase
-
-
-
-
PGE2-9-OR
-
-
-
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prostaglandin 9-ketoreductase
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-
-
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prostaglandin E 9-ketoreductase
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-
-
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prostaglandin E2-9-oxoreductase
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-
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reductase, 15-hydroxy9-oxoprostaglandin
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-
-
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additional information
-
enzyme is a member of the AKR aldo-keto reductase superfamily
PGE2 9-ketoreductase
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-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ = (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate + NADPH + H+
enzyme is identical to carbonyl reductase
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
redox reaction
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-
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oxidation
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reduction
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-
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SYSTEMATIC NAME
IUBMB Comments
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate:NADP+ 9-oxidoreductase
Reduces prostaglandin E2 to prostaglandin F2alpha. A number of other 9-oxo- and 15-oxo-prostaglandin derivatives can also be reduced to the corresponding hydroxy compounds. May be identical with EC 1.1.1.197 15-hydroxyprostaglandin dehydrogenase (NADP+).
CAS REGISTRY NUMBER
COMMENTARY
42613-35-4
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate + NAD(P)+
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H
-
-
-
-
ir
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate + NADPH + H+
-
enzyme is pro(S) stereo selective with regard to hydrogen transfer from NADPH
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-
r
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
(5Z,13E)-9alpha,11alpha-dihydroxy-15-oxoprosta-5,13-dienoate + NADPH + H+
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-
-
-
ir
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADP+
(5Z,13E)-9alpha-hydroxy-11,15-dioxoprosta-5,13-dienoate + NADPH
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-
-
-
ir
prostaglandin H2 + NAD(P)H + H+
prostaglandin F2alpha + NAD(P)+
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-
product is involved in bronchial, vascular, and arterial smooth muscle contraction
-
r
additional information
?
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-
broad substrate specificity, enzyme shows 15-hydroxy dehydrogenase activity in presence of NADP+ or NAD+
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
prostaglandin H2 + NAD(P)H + H+
prostaglandin F2alpha + NAD(P)+
-
-
product is involved in bronchial, vascular, and arterial smooth muscle contraction
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r
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
peri- and postimplantation conceptus. Prostaglandin H synthase and prostaglandin E synthase are down-regulated, and prostaglandin G synthase, prostaglandin F synthase and carbonyl reductase/prostaglandin 9-reductase are down-regulated in conceptuses during trophoblasic elongation. After initiation of implantation, expression of prostaglandin G synthase, prostaglandin F synthase and carbonyl reductase/prostaglandin 9-reductase in conceptuses increases and remains higher until days 24-25 of pregnancy
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high expression of conceptus and endometrial terminal CBR1 after initiation of blastocyst attachment suggest its involvement in early placentation
at days 12-14 of pregnancy and the estrous cycle, the expression of prostaglandin E2 synthase, prostaglandin F synthase, and carbonyl reductase/prostaglandin 9-ketoreductase genes and prostaglandin E2 synthase/prostaglandin F synthase ratio are significantly higher in corpora lutea of the pregnant gilts compared to the corpora lutea from the parallel ovaries of the cyclic gilts. There is no difference in expression of prostaglandin E2 synthase, prostaglandin F synthase, and carbonyl reductase/prostaglandin 9-ketoreductase genes and prostaglandin E2 synthase/prostaglandin F synthase ratio between corpora lutea ipsi- and contralateral to the uterine horn with the developing embryos
expression of CBR1 mRNA and protein is constant during the cycle and pregnancy
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). CBR1_PIG
289
0
31693
Swiss-Prot
Mitochondrion (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
32000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
peri- and postimplantation conceptus. Prostaglandin H synthase and prostaglandin E synthase are down-regulated, and prostaglandin G synthase, prostaglandin F synthase and carbonyl reductase/prostaglandin 9-reductase are down-regulated in conceptuses during trophoblasic elongation. After initiation of implantation, expression of prostaglandin G synthase, prostaglandin F synthase and carbonyl reductase/prostaglandin 9-reductase in conceptuses increases and remains higher until days 24-25 of pregnancy
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Schieber, A.; Frank, R.W.; Ghisla, S.
Purification and properties of prostaglandin 9-ketoreductase from pig and human kidney. Identity with human carbonyl reductase
Eur. J. Biochem.
206
491-502
1992
Homo sapiens, Sus scrofa
Schieber, A.; Ghisla, S.
Prostaglandin 9-ketoreductase from pig and human kidney: purification, properties and identity with human carbonyl reductase
Eicosanoids
5
S37-38
1992
Homo sapiens, Sus scrofa
Watanabe, K.
Prostaglandin F synthase
Prostaglandins
68-69
401-407
2002
Bos taurus, Gallus gallus, Homo sapiens, Oryctolagus cuniculus, Sus scrofa
Waclawik, A.; Ziecik, A.J.
Differential expression of prostaglandin (PG) synthesis enzymes in conceptus during peri-implantation period and endometrial expression of carbonyl reductase/PG 9-ketoreductase in the pig
J. Endocrinol.
194
499-510
2007
Sus scrofa (Q28960)
Wasielak, M.; Glowacz, M.; Kaminska, K.; Waclawik, A.; Bogacki, M.
The influence of embryo presence on prostaglandins synthesis and prostaglandin E2 and F2alpha content in corpora lutea during periimplantation period in the pig
Mol. Reprod. Dev.
75
1208-1216
2008
Sus scrofa (Q28960)
Waclawik, A.; Jabbour, H.N.; Blitek, A.; Ziecik, A.J.
Estradiol-17beta, prostaglandin E2 (PGE2), and the PGE2 receptor are involved in PGE2 positive feedback loop in the porcine endometrium
Endocrinology
150
3823-3832
2009
Sus scrofa (Q28960)
Ziecik, A.J.; Waclawik, A.; Bogacki, M.
Conceptus signals for establishment and maintenance of pregnancy in pigs - lipid signaling system
Exp. Clin. Endocrinol. Diabetes
116
443-449
2008
Sus scrofa
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