Information on EC 1.1.1.181 - cholest-5-ene-3beta,7alpha-diol 3beta-dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Euarchontoglires

EC NUMBER
COMMENTARY
1.1.1.181
-
RECOMMENDED NAME
GeneOntology No.
cholest-5-ene-3beta,7alpha-diol 3beta-dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cholest-5-ene-3beta,7alpha-diol + NAD+ = 7alpha-hydroxycholest-4-en-3-one + NADH + H+
show the reaction diagram
Highly specific for 3beta-hydroxy-C27-steroids with DELTA5-double bond
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
bile acid biosynthesis, neutral pathway
-
-
bile acid biosynthesis, neutral pathway
-
-
Metabolic pathways
-
-
Primary bile acid biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
cholest-5-ene-3beta,7alpha-diol:NAD+ 3-oxidoreductase
Highly specific for 3beta-hydroxy-C27-steroids with Delta5-double bond.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3beta-hydroxy-DELTA5-C27-steroid oxidoreductase
-
-
-
-
5-cholestene-3beta,7alpha-diol 3beta-dehydrogenase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
56626-16-5
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
cholest-5-ene-3beta,7alpha-diol + NAD+
7alpha-hydroxycholest-4-en-3-one + NADH
show the reaction diagram
-
highly specific
-
r
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cholest-5-ene-3beta,7alpha-diol + NAD+
7alpha-hydroxycholest-4-en-3-one + NADH
show the reaction diagram
-
highly specific
-
r
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NAD+
-
can not be replaced by NADP+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
FMN
-
30% inhibition at 0.1 mM
Quinacrine
-
slight inhibition at 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.045
cholest-5-ene-3beta,7alpha-diol
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7 - 7.5
-
in potassium phosphate buffer
9
-
in Tris-HCl buffer
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
46000
-
gel filtration
286129
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
-
1 * 46000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity, chromatography techniques
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
molecular analysis of 15 patients with neonatal cholestasis reveals 12 different mutations in HSD3B7 gene, 10 of them leading to complete inactivation of enzyme
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
-
molecular analysis of 15 patients with neonatal cholestasis reveals 12 different mutations in HSD3B7 gene, 10 of them leading to complete inactivation of enzyme