Information on EC 1.1.1.173 - L-rhamnose 1-dehydrogenase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.1.173
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RECOMMENDED NAME
GeneOntology No.
L-rhamnose 1-dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-rhamnofuranose + NAD+ = L-rhamno-1,4-lactone + NADH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
-
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redox reaction
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-
-
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reduction
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Fructose and mannose metabolism
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L-rhamnose degradation II
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L-rhamnose degradation III
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Microbial metabolism in diverse environments
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degradation of hexoses
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SYSTEMATIC NAME
IUBMB Comments
L-rhamnofuranose:NAD+ 1-oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
52227-67-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain NBRC0083, gene LRA1
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Manually annotated by BRENDA team
strain NBRC0083, gene LRA1
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Manually annotated by BRENDA team
strain NRC5568, gene RHA1
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-lyxose + NAD+
? + NADH
show the reaction diagram
L-mannose + NAD+
? + NADH
show the reaction diagram
L-rhamnose + NAD+
L-rhamno-1,4-lactone + NADH
show the reaction diagram
L-rhamnose + NADP+
L-rhamno-1,4-lactone + NADPH
show the reaction diagram
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-
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-rhamnose + NAD+
L-rhamno-1,4-lactone + NADH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
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about 30fold less activity than with NAD+
additional information
-
no activity with NADPH
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
p-mercuribenzoate
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-
additional information
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D-glucose suppresses the enzyme
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
enzyme transcription in Pichia stipitis is induced during growth on L-rhamnose but not on other carbon sources
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5
L-Lyxose
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pH 8.0, 30°C, recombinant enzyme
25
L-Mannose
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pH 8.0, 30°C, recombinant enzyme
0.2 - 9.35
L-rhamnose
additional information
additional information
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substrate specificity, overview
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1510 - 2860
L-rhamnose
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.02
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after induction by growth on L-rhamnose
0.6
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purified recombinant enzyme
1.11
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substrate L-rhamnose, cosubstrate NADP+, pH 9.5, 50°C
1.22
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substrate L-mannose, cosubstrate NAD+, pH 9.5, 50°C
22
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substrate L-lyxose, cosubstrate NAD+, pH 9.5, 50°C
29.9
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purified native enzyme
33
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substrate L-rhamnose, cosubstrate NAD+, pH 9.5, 50°C
50.4
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purified recombinant enzyme
53.3
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purified recombinant enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000
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x * 25000, SDS-PAGE
30000
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x * 30000, recombinant enzyme, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme 42.8fold by anion exchange chromatography, ultrafiltration, gel filtration, hydroxyapatite chromatography, and again gel filtration, recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
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recombinant enzyme in analytical amounts from Saccharomyces cerevisiae by anion exchange chromatography, and native and SDS-PAGE, tagging of the enzyme strongly affects its activity, overview
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recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene LRA1, DNA and amino acid sequence determination and analysis, genetic organization, functional expression of His-tagged enzyme in Escherichia coli
gene RHA1, DNA and amino acid sequence determination and analysis, expression in Saccharomyces cereviaie
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