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Information on EC 1.1.1.153 - sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming) and Organism(s) Mus musculus and UniProt Accession Q64105

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IUBMB Comments
This enzyme catalyses the final step in the de novo synthesis of tetrahydrobiopterin from GTP. The enzyme, which is found in higher animals and some fungi and bacteria, produces the erythro form of tetrahydrobiopterin. cf. EC 1.1.1.325, sepiapterin reductase (L-threo-7,8-dihydrobiopterin forming).
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This record set is specific for:
Mus musculus
UNIPROT: Q64105
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
akr1b1, sepiapterin reductase, mdspr, mdspi16 protein, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
AKR1B1
-
-
reductase, sepiapterin
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
L-erythro-7,8-dihydrobiopterin:NADP+ oxidoreductase
This enzyme catalyses the final step in the de novo synthesis of tetrahydrobiopterin from GTP. The enzyme, which is found in higher animals and some fungi and bacteria, produces the erythro form of tetrahydrobiopterin. cf. EC 1.1.1.325, sepiapterin reductase (L-threo-7,8-dihydrobiopterin forming).
CAS REGISTRY NUMBER
COMMENTARY hide
9059-48-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
show the reaction diagram
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
show the reaction diagram
6-pyruvoyl tetrahydropterin + NADPH + H+
1'-oxo-2'-L-hydroxypropyl-tetrahydropterin + NADP+
show the reaction diagram
-
-
-
-
?
6-pyruvoyl-5,6,7,8-tetrahydropterin + NADPH
tetrahydrobiopterin + NADP+
show the reaction diagram
-
-
-
-
r
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
show the reaction diagram
additional information
?
-
-
final step in the synthesis of tetrahydrobiopterin
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
show the reaction diagram
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
show the reaction diagram
6-pyruvoyl-5,6,7,8-tetrahydropterin + NADPH
tetrahydrobiopterin + NADP+
show the reaction diagram
-
-
-
-
r
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
show the reaction diagram
-
-
-
-
r
additional information
?
-
-
final step in the synthesis of tetrahydrobiopterin
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N-acetylserotonin
-
N-chloroserotonin
strong inhibitor
N-methoxyacetylserotonin
strong competitive inhibitor
oxaloacetate
-
sulfasalazine
-
N-acetylserotonin
-
activity is completely inhibited by the addition of 0.5 mM N-acetylserotonin
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
-
not in leukocytes and ascites tumor cells
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
enzyme inhibition reduces pain hypersensitivity in a mouse model of joint inflammation
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SPRE_MOUSE
261
0
27883
Swiss-Prot
other Location (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
-
SDS-PAGE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
vapour diffusion technique
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
construction of enzyme knockout mice, the SPR null mice show partial lethality and highly reduced tetrahydrobiopterin content, e.g. less than 10% of wild-type levels in the brain, and monoamine contents. Spr-null mice exhibit severe monoamine deficiencies and a tremor-like phenotype after weaning, pteridine contents in the tissues of Spr mutant mice, phenotype, overview
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, desalted extracts, several weeks without significant loss of activity
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant protein of Escherichia coli
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant protein expressed in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Katoh, S.; Arai, Y.; Taketani, T.; Yamada, S.
Sepiapterin reductase in blood of various animals and of leukemic rats
Biochim. Biophys. Acta
370
378-388
1974
Canis lupus familiaris, Cavia porcellus, Gallus gallus, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus, Rattus norvegicus Donryu, Rattus norvegicus Sprague-Dawley, Sus scrofa
Manually annotated by BRENDA team
Kerler, F.; Hultner, L.; Ziegler, I.; Katzenmaier, G.; Bacher, A.
Analysis of the tetrahydrobiopterin synthesizing system during maturation of murine reticulocytes
J. Cell. Physiol.
142
268-271
1990
Mus musculus
Manually annotated by BRENDA team
Auerbach, G.; Herrmann, A.; Gutlich, M.; Fischer, M.; Jacob, U.; Bacher, A.; Huber, R.
The 1.25 A crystal structure of sepiapterin reductase reveals its binding mode to pterins and brain neurotransmitters
EMBO J.
16
7219-7230
1997
Mus musculus (Q64105), Mus musculus
Manually annotated by BRENDA team
Ikemoto, K.; Suzuki, T.; Ichinose, H.; Ohye, T.; Nishimura, A.; Nishi, K.; Nagatsu, I.; Nagatsu, T.
Localization of sepiapterin reductase in the human brain
Brain Res.
954
237-246
2002
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Takazawa, C.; Fujimoto, K.; Homma, D.; Sumi-Ichinose, C.; Nomura, T.; Ichinose, H.; Katoh, S.
A brain-specific decrease of the tyrosine hydroxylase protein in sepiapterin reductase-null mice - as a mouse model for Parkinsons disease
Biochem. Biophys. Res. Commun.
367
787-792
2008
Mus musculus
Manually annotated by BRENDA team
Hirakawa, H.; Sawada, H.; Yamahama, Y.; Takikawa, S.; Shintaku, H.; Hara, A.; Mase, K.; Kondo, T.; Iino, T.
Expression analysis of the aldo-keto reductases involved in the novel biosynthetic pathway of tetrahydrobiopterin in human and mouse tissues
J. Biochem.
146
51-60
2009
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Fujita, M.; Scheffer, D.D.L.; Turnes, B.L.; Cronin, S.J.F.; Latremoliere, A.; Costigan, M.; Woolf, C.J.; Latini, A.; Andrews, N.A.
Sepiapterin reductase inhibition selectively reduces inflammatory joint pain and increases urinary sepiapterin
Arthritis Rheumatol.
72
57-66
2020
Mus musculus (Q64105), Mus musculus
Manually annotated by BRENDA team