Information on EC 1.1.1.127 - 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.127
-
RECOMMENDED NAME
GeneOntology No.
2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-dehydro-3-deoxy-D-gluconate + NAD+ = (4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH + H+
show the reaction diagram
The enzyme from Pseudomas acts equally well on NAD+ or NADP+, while that from Erwinia chrysanthemi and E. coli is more specific for NAD+
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
-
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redox reaction
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-
-
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reduction
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Pentose and glucuronate interconversions
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4-deoxy-L-threo-hex-4-enopyranuronate degradation
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3,6-anhydro-alpha-L-galactopyranose degradation
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SYSTEMATIC NAME
IUBMB Comments
2-dehydro-3-deoxy-D-gluconate:NAD+ 5-oxidoreductase
The enzyme from Pseudomonas acts equally well on NAD+ or NADP+, while that from Erwinia chrysanthemi and Escherichia coli is more specific for NAD+.
CAS REGISTRY NUMBER
COMMENTARY hide
37250-56-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,5-didehydro-3-deoxy-D-gluconate + NADH + H+
2-dehydro-3-deoxy-D-gluconate + NAD+
show the reaction diagram
-
-
-
?
2,5-didehydro-3-deoxy-D-gluconate + NADPH + H+
2-dehydro-3-deoxy-D-gluconate + NADP+
show the reaction diagram
-
-
-
?
2-dehydro-3-deoxy-D-gluconate + NAD+
(4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
show the reaction diagram
pyruvate + NAD+
? + NADH
show the reaction diagram
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10% of the activity compared to 2-dehydro-3-deoxy-D-gluconate
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2,5-didehydro-3-deoxy-D-gluconate + NADH + H+
2-dehydro-3-deoxy-D-gluconate + NAD+
show the reaction diagram
A0A093RP61
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-
-
?
2,5-didehydro-3-deoxy-D-gluconate + NADPH + H+
2-dehydro-3-deoxy-D-gluconate + NADP+
show the reaction diagram
A0A093RP61
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-
-
?
2-dehydro-3-deoxy-D-gluconate + NAD+
(4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
NADPH
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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20% activation at 1 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
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72% inhibition at 0.01 mM
ATP
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30% inhibition for both reduction and oxidation reaction at 1 mM
p-chloromercuribenzoate
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50% inhibition at 0.02 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.34
(4S)-4,6-dihydroxy-2,5-dioxohexanoate
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7.7
2-dehydro-3-deoxy-D-gluconate
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0.0021 - 0.03
NADH
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.5
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reduction reaction
10
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oxidation reaction
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
in complex with NAD+, hanging drop vapor diffusion method, using 5% (w/v) polyethylene glycol 4000, and 10% (v/v) isopropanol
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
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stable for 15 h
40
the enzyme shows more than 70% activity at 40C for 5 min
46
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half-life: 10 min, NADH increases thermal stability
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
1 mM NAD+ or NADP+ increase stability
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 2 mM DTT, 6 months
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0-3C, 1 mM NAD+ or NADP+, 50-67% retaining activity after 5 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity, 2step chromatography
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Toyopearl DEAE-650 M column chromatography, Toyopearl butyl-650 M column chromatography, Q-Sepharose column chromatography, and Superdex 200 pg gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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