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Information on EC 1.1.1.121 - aldose 1-dehydrogenase (NAD+)

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EC Tree
IUBMB Comments
Acts on D-glucose, 2-deoxy- and 6-deoxy-D-glucose, D-galactose, 6-deoxy-D-galactose, 2-deoxy-L-arabinose and D-xylose.
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This record set is specific for:
UNIPROT: Q5SLC4
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Word Map
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
aldose dehydrogenase, ta0754, aldohexose dehydrogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aldose dehydrogenase
-
-
-
-
dehydrogenase, D-aldohexose
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
D-aldose:NAD+ 1-oxidoreductase
Acts on D-glucose, 2-deoxy- and 6-deoxy-D-glucose, D-galactose, 6-deoxy-D-galactose, 2-deoxy-L-arabinose and D-xylose.
CAS REGISTRY NUMBER
COMMENTARY hide
9076-61-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-indanol + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
(R)-tetralol + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
(S)-(+)-1,2,3,4-tetrahydro-1-naphthol + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
(S)-(+)-1-indanol + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
(S)-indanol + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
(S)-tetralol + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
1-acenaphthenol + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
2-cyclohexen-1-ol + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
2-deoxy-D-galactose + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
2-deoxy-D-glucose + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
4-chromanol + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
6-deoxy-D-glucose
? + NADH + H+
show the reaction diagram
-
-
-
?
alpha-tetralone + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
?
cellobiose + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
cis-benzene dihydrodiol + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
D-fucose + NAD+
D-fucono-delta-lactone + NADH + H+
show the reaction diagram
-
-
-
?
D-galactosamine + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
D-galactose + NAD+
D-galactono-gamma-lactone + NADH + H+
show the reaction diagram
-
-
-
?
D-glucosamine + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
D-glucose + NAD+
D-glucono-delta-lactone + NADH + H+
show the reaction diagram
-
-
-
?
D-talose + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
D-xylose + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
glucono-delta-lactone + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
?
isatin + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
?
L-arabinose + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
L-fucose + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
lactose + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
phenyl-1,2-propanedione + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose
2,3-benzofuran
uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose
Hexestrol
uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose
indole
uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.17
(S)-tetralol
-
0.14
1-Acenaphthenol
-
3.7
2-Cyclohexen-1-ol
-
33
2-deoxy-D-galactose
-
300
2-deoxy-D-glucose
-
178
6-deoxy-D-glucose
-
1.9
alpha-tetralone
-
27
cis-benzene dihydrodiol
-
2 - 3
D-galactosamine
-
139
D-glucosamine
-
2
phenyl-1,2-propanedione
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.167
(R)-indanol
-
0.075
(R)-tetralol
-
138.7
(S)-indanol
-
161.7
(S)-tetralol
-
433.3
1-Acenaphthenol
-
3.5
2-Cyclohexen-1-ol
-
12.8
2-deoxy-D-galactose
-
6.93
2-deoxy-D-glucose
-
6.35
6-deoxy-D-glucose
-
3.9
alpha-tetralone
-
7.383
cis-benzene dihydrodiol
-
0.3117
D-galactosamine
-
0.6217
D-glucosamine
-
16.83
phenyl-1,2-propanedione
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.025
1,10-phenanthroline
-
0.042
2,3-benzofuran
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.18
1,10-phenanthroline
Thermus thermophilus HB8
-
0.2
2,3-benzofuran
Thermus thermophilus HB8
-
1.2
catechol
Thermus thermophilus HB8
-
0.063
Hexestrol
Thermus thermophilus HB8
-
1.9
indazole
Thermus thermophilus HB8
-
0.48
indole
Thermus thermophilus HB8
-
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q5SLC4_THET8
Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
256
0
26961
TrEMBL
Secretory Pathway (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
gel filtration
27000
4 * 27000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
4 * 27000, SDS-PAGE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by oil-microbatch method, at 1.65 A resolution. TAD follows an ordered sequential mechanism in which the coenzyme binds first
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D242N
mutation results in a large decrease in thermostability of TAD without dissociation into dimer or monomer
F141A
mutation results in decrease in thermostability of TAD without dissociation into dimer or monomer
F249A
mutation results in decrease in thermostability of TAD without dissociation into dimer or monomer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
85
approximately 40% of the activity is inactivated by the incubation of the enzyme solution (0.3 mg/ml in 10 mM potassium phosphate, pH 7.0) for 15 min at 95°C
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by sonication, heating at 70°C for 10 min, ion-exchange chromatography, gel filtration and ultrafiltration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
into vector pET-11a and overexpressed in Escherichia coli BL21 (DE3) cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Asada, Y.; Endo, S.; Inoue, Y.; Mamiya, H.; Hara, A.; Kunishima, N.; Matsunaga, T.
Biochemical and structural characterization of a short-chain dehydrogenase/reductase of Thermus thermophilus HB8: a hyperthermostable aldose-1-dehydrogenase with broad substrate specificity
Chem. Biol. Interact.
178
117-126
2009
Thermus thermophilus HB8 (Q5SLC4), Thermus thermophilus HB8
Manually annotated by BRENDA team