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(R)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
r
(R)-2-pentanol + NAD+
2-pentanone + NADH + H+
-
-
-
r
(S)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
r
(S)-2-pentanol + NAD+
2-pentanone + NADH + H+
-
-
-
r
1-butanol + NAD+
butanal + NADH + H+
-
-
-
r
1-heptanol + NAD+
1-heptanal + NADH + H+
-
-
-
r
1-hexanol + NAD+
1-hexanal + NADH + H+
-
-
-
r
1-pentanol + NAD+
1-pentanal + NADH + H+
-
-
-
r
1-propanol + NAD+
propanal + NADH + H+
-
-
-
r
2-(3,5-dimethylphenyl)propanal + NADH + H+
(S)-2-(3,5-dimethylphenyl)propanol + NAD+
18 h, 99% yield, 90% enantiomeric excess
-
-
?
2-(3-benzoylphenyl)propanal + NADH + H+
(S)-2-(3-benzoylphenyl)propanol + NAD+
18 h, 85% yield, 95% enantiomeric excess
-
-
?
2-(3-fluorobiphenyl-4-yl)propanal + NADH + H+
(S)-2-(3-(fluoro)biphenyl-4-yl)propanol + NAD+
18 h, 77% yield, 97% enantiomeric excess
-
-
?
2-(3-phenoxyphenyl)propanal + NADH + H+
(S)-2-((3-phenoxy)phenyl)propanol + NAD+
18 h, 85% yield, 95% enantiomeric excess
-
-
?
2-(4-isobutylphenyl)propanal + NADH + H+
(S)-2-(4-isobutylphenyl)propanol + NAD+
18 h, 92% yield, 99% enantiomeric excess
-
-
?
2-(4-trifluoromethylphenyl)propanal + NADH + H+
(S)-2-(4-trifluoromethyl)phenylpropanol + NAD+
18 h, 55% yield, 98% enantiomeric excess
-
-
?
2-(6-methoxynaphthalen-2-yl)propanal + NADH + H+
(S)-2-(6-methoxynaphthalen-2-yl)propan-1-ol + NAD+
18 h, 96% yield, 98% enantiomeric excess
-
-
?
2-(naphthalen-1-yl)propanal + NADH + H+
(S)-2-(naphthalen-1-yl)propanol + NAD+
18 h, 90% yield, 80% enantiomeric excess
-
-
?
2-(naphthalen-2-yl)propanal + NADH + H+
(S)-2-(naphthalen-2-yl)propanol + NAD+
18 h, 57% yield, 94% enantiomeric excess
-
-
?
2-ethoxyethanol + NAD+
2-ethoxyacetaldehyde + NADH + H+
-
-
-
r
2-phenylpropanal + NADH + H+
(S)-2-phenylpropanol + NAD+
18 h, 74% yield, 98% enantiomeric excess
-
-
?
2-[3-(2-phenyl-1,3-dioxolan-2-yl)phenyl]propanal + NADH + H+
(S)-2-(3-(2-phenyl-1,3-dioxolan-2-yl)phenyl)propanol + NAD+
18 h, 95% yield, 61% enantiomeric excess
-
-
?
3-pentanol + NAD+
3-pentanone + NADH + H+
-
-
-
r
4-methoxybenzyl alcohol + NAD+
4-methoxybenzaldehyde + NADH + H+
-
-
-
r
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
r
cyclohexanol + NAD+
cyclohexanone + NADH + H+
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
isobutyraldehyde + NADH + H+
isobutanol + NAD+
-
-
-
r
n-butanol + NAD+
butyraldehyde + NADH + H+
-
-
-
r
pentan-3-ol + NAD+
3-pentanone + NADH + H+
-
-
-
r
trans-cinnamaldehyde + NADH + H+
cinnamyl alcohol + NAD+
-
-
-
r
1-propanol + NAD+
propanal + NADH + H+
-
-
-
-
?
2-methylcyclohexanol + NAD+
(+)-2-methylcyclohexanone + NADH + H+
-
-
-
-
r
2-propanol + NAD+
2-propanone + NADH + H+
-
-
-
-
?
3-bromobenzyl alcohol + NAD+
3-bromobenzaldehyde + NADH + H+
-
-
-
-
?
3-bromobenzylalcohol + NAD+
3-bromobenzaldehyde + NADH + H+
-
-
-
-
?
3-methoxybenzyl alcohol + NAD+
3-methoxybenzaldehyde + NADH + H+
-
-
-
-
?
3-methoxybenzylalcohol + NAD+
3-methoxybenzaldehyde + NADH + H+
-
-
-
-
?
3-methylbutan-2-one + NADH
3-methylbutan-2-ol + NAD+
-
-
-
-
?
3-methylbutan-2-one + NADH + H+
3-methylbutan-2-ol + NAD+
-
-
-
-
?
3-methylcyclohexanol + NAD+
3-methylcyclohexanone + NADH
-
-
-
-
r
3-methylcyclohexanone + NADH
3-methylcyclohexanol + NAD+
-
-
-
-
r
4-bromobenzyl alcohol + NAD+
4-bromobenzaldehyde + NADH + H+
-
-
-
-
?
4-bromobenzylalcohol + NAD+
4-bromobenzaldehyde + NADH + H+
-
-
-
-
?
4-carboxybenzaldehyde + NADH + H+
4-carboxybenzyl alcohol + NAD+
-
-
-
-
?
4-carboxybenzaldehyde + NADH + H+
4-carboxybenzylalcohol + NAD+
-
-
-
-
?
4-methoxybenzyl alcohol + NAD+
4-methoxybenzaldehyde + NADH + H+
-
-
-
-
r
4-methoxybenzylalcohol + NAD+
4-methoxybenzaldehyde + NADH + H+
-
-
-
-
r
4-nitrobenzaldehyde + NADH + H+
4-nitrobenzyl alcohol + NAD+
-
-
-
-
?
4-nitrobenzaldehyde + NADH + H+
4-nitrobenzylalcohol + NAD+
-
-
-
-
?
acetone + NADH
isopropanol + NAD+
-
-
-
r
anisaldehyde + NADH + H+
anisic alcohol + NAD+
-
-
-
-
?
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
benzylalcohol + NAD+
benzaldehyde + NADH + H+
butan-2-ol + NAD+
butan-2-one + NADH
-
-
-
-
?
butan-2-one + NADH
butan-2-ol + NAD+
-
-
-
-
r
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butyraldehyde + NADH + H+
n-butanol + NAD+
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH + H+
-
-
-
-
?
cyclopetanone + NADH + H+
cyclopentanol + NAD+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
iso-propanol + NAD+
isopropanal + NADH + H+
-
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
-
-
-
r
n-propanol + NAD+
propanal + NADH + H+
-
-
-
?
pentan-2-ol + NAD+
2-pentanone + NADH
-
-
-
-
?
pentan-3-ol + NAD+
3-pentanone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propanol + NAD+
propionaldehyde + NADH + H+
-
-
-
-
?
additional information
?
-
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
r
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
r
benzylalcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
r
benzylalcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
additional information
?
-
the enzyme undergoes a substantial conformational change in the apo-holo transition, accompanied by loop movements at the domain interface
-
-
?
additional information
?
-
-
the enzyme undergoes a substantial conformational change in the apo-holo transition, accompanied by loop movements at the domain interface
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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0.41 - 37.4
(R)-2-butanol
0.22 - 11.7
(R)-2-pentanol
0.012 - 29.8
(S)-2-butanol
0.07 - 8.8
(S)-2-pentanol
0.25
2-ethoxyethanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.27
3-Pentanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.07 - 1.3
4-methoxybenzyl alcohol
0.12 - 3.8
benzyl alcohol
0.07 - 0.27
Butyraldehyde
0.26 - 0.76
Isobutyraldehyde
0.009 - 0.23
trans-cinnamaldehyde
0.25
(+/-)-3-methylcyclohexanone
-
-
0.021
2-Methylcyclohexanol
-
-
0.14 - 0.9
3-bromobenzyl alcohol
0.14
3-bromobenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
1.2 - 3.3
3-Methoxybenzyl alcohol
1.2
3-methoxybenzylalcohol
7.5
3-methylbutan-2-one
-
-
0.12 - 0.4
4-bromobenzyl alcohol
0.12
4-bromobenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.28
4-carboxybenzaldehyde
0.024 - 0.86
4-methoxybenzaldehyde
0.23 - 1.4
4-methoxybenzyl alcohol
1.3
4-methoxybenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.0025 - 0.09
Anisaldehyde
2.4
iso-propanol
2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C
0.19
n-Propanol
2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C
0.41
(R)-2-butanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
37.4
(R)-2-butanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.22
(R)-2-pentanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
11.7
(R)-2-pentanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.012
(S)-2-butanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
29.8
(S)-2-butanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.07
(S)-2-pentanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
8.8
(S)-2-pentanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.08
1-butanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.6
1-butanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.038
1-Heptanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.21
1-Heptanol
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.67
1-Heptanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.035
1-Hexanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.04
1-Hexanol
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.88
1-Hexanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.027
1-Pentanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.26
1-Pentanol
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.9
1-Pentanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.24
1-propanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
6.5
1-propanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.07
4-methoxybenzyl alcohol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.12
4-methoxybenzyl alcohol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.3
4-methoxybenzyl alcohol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.03
benzaldehyde
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.07
benzaldehyde
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.28
benzaldehyde
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.12
benzyl alcohol
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.26
benzyl alcohol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
3.8
benzyl alcohol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.07
Butyraldehyde
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.13
Butyraldehyde
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.27
Butyraldehyde
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.03
Cyclohexanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
4.5
Cyclohexanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
4.6
ethanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
66
ethanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.26
Isobutyraldehyde
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.33
Isobutyraldehyde
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.76
Isobutyraldehyde
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.5
NAD+
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
4
NAD+
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
50
NAD+
Km above 50 mM, mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.008
NADH
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.01
NADH
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.75
NADH
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.009
trans-cinnamaldehyde
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.09
trans-cinnamaldehyde
mutant enzyme W95L,, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.23
trans-cinnamaldehyde
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.33
1-propanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.33
1-propanol
-
native enzyme, at 55°C
0.6
1-propanol
-
wild type enzyme, at 65°C
1.7
1-propanol
-
mutant enzyme N249Y, at 65°C
2.4
1-propanol
-
carboxymethylated enzyme, at 55°C
0.6
2-propanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.6
2-propanol
-
native enzyme, at 55°C
53
2-propanol
-
carboxymethylated enzyme, at 55°C
0.14
3-bromobenzyl alcohol
-
native enzyme, at 55°C
0.9
3-bromobenzyl alcohol
-
carboxymethylated enzyme, at 55°C
1.2
3-Methoxybenzyl alcohol
-
native enzyme, at 55°C
3.3
3-Methoxybenzyl alcohol
-
carboxymethylated enzyme, at 55°C
1.2
3-methoxybenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
1.2
3-methoxybenzylalcohol
-
in the presence of 5 mM benzylalcohol, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.12
4-bromobenzyl alcohol
-
native enzyme, at 55°C
0.4
4-bromobenzyl alcohol
-
carboxymethylated enzyme, at 55°C
0.28
4-carboxybenzaldehyde
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
0.28
4-carboxybenzaldehyde
-
native enzyme, at 55°C
0.024
4-methoxybenzaldehyde
-
wild type enzyme, at 65°C
0.14
4-methoxybenzaldehyde
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
0.14
4-methoxybenzaldehyde
-
native enzyme, at 55°C
0.86
4-methoxybenzaldehyde
-
mutant enzyme N249Y, at 65°C
0.23
4-methoxybenzyl alcohol
-
wild type enzyme, at 65°C
1.24
4-methoxybenzyl alcohol
-
mutant enzyme N249Y, at 65°C
1.3
4-methoxybenzyl alcohol
-
native enzyme, at 55°C
1.4
4-methoxybenzyl alcohol
-
carboxymethylated enzyme, at 55°C
0.0025
Anisaldehyde
-
-
0.03
benzaldehyde
-
wild type enzyme, at 65°C
0.2
benzaldehyde
-
carboxymethylated enzyme, at 55°C
0.26
benzaldehyde
-
mutant enzyme N249Y, at 65°C
0.3
benzaldehyde
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
0.3
benzaldehyde
-
native enzyme, at 55°C
0.013
benzyl alcohol
-
-
0.26
benzyl alcohol
-
wild type enzyme, at 65°C
0.8
benzyl alcohol
-
native enzyme, at 55°C
1.5
benzyl alcohol
-
mutant enzyme N249Y, at 65°C
2
benzyl alcohol
-
carboxymethylated enzyme, at 55°C
0.44
benzylalcohol
2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C
0.8
benzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.03
Cyclohexanol
-
wild type enzyme, at 65°C
0.045
Cyclohexanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.045
Cyclohexanol
-
native enzyme, at 55°C
0.23
Cyclohexanol
-
carboxymethylated enzyme, at 55°C
1.9
Cyclohexanol
-
mutant enzyme N249Y, at 65°C
0.26
ethanol
-
-
0.7
ethanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.7
ethanol
-
native enzyme, at 55°C
1.33
ethanol
2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C
184
ethanol
-
carboxymethylated enzyme, at 55°C
0.2
NAD+
-
in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.2
NAD+
-
native enzyme, at 55°C
0.5
NAD+
-
carboxymethylated enzyme, at 55°C
0.5
NAD+
-
wild type enzyme, at 65°C, in the presence of benzyl alcohol
12.4
NAD+
-
mutant enzyme N249Y, at 65°C, in the presence of benzyl alcohol
0.013
NADH
-
wild type enzyme, at 65°C, in the presence of benzaldehyde
0.03
NADH
-
in 50 mM Tris-HCl (pH 7.5) containing 0.25 mM benzaldehyde, at 65°C
0.03
NADH
-
native enzyme, at 55°C
0.04
NADH
-
carboxymethylated enzyme, at 55°C
0.21
NADH
-
mutant enzyme N249Y, at 65°C, in the presence of benzaldehyde
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1.6
2-ethoxyethanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.5
3-Pentanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.37 - 16.8
4-methoxybenzyl alcohol
0.36 - 19.7
Butyraldehyde
0.82 - 29
Isobutyraldehyde
0.21 - 18.9
trans-cinnamaldehyde
1.2 - 2
3-bromobenzyl alcohol
1.2
3-bromobenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
1.4 - 16
3-Methoxybenzyl alcohol
1.2 - 1.4
3-methoxybenzylalcohol
7.72
3-methyl-cyclohexanol
-
-
1.22
3-methylbutan-2-one
-
-
2.17
3-methylcyclohexanone
-
-
1.1 - 3.5
4-bromobenzyl alcohol
1.1
4-bromobenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.7
4-carboxybenzaldehyde
0.6 - 20.5
4-methoxybenzaldehyde
1.5 - 25.5
4-methoxybenzyl alcohol
1.5
4-methoxybenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
1.4 - 21.7
benzyl alcohol
1.4
benzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
1
(R)-2-butanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.7
(R)-2-butanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.1
(R)-2-pentanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.9
(R)-2-pentanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.2
(S)-2-butanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
4.8
(S)-2-butanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
2.6
(S)-2-pentanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
6.7
(S)-2-pentanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
3.1
1-butanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
15.1
1-butanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.51
1-Heptanol
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.6
1-Heptanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
11.9
1-Heptanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.42
1-Hexanol
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
2
1-Hexanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
13.3
1-Hexanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.53
1-Pentanol
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
2.5
1-Pentanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
16.3
1-Pentanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
2.5
1-propanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
15.7
1-propanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.37
4-methoxybenzyl alcohol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
2.9
4-methoxybenzyl alcohol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
16.8
4-methoxybenzyl alcohol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.36
benzaldehyde
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
11.3
benzaldehyde
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
13.1
benzaldehyde
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.35
benzyl alcohol
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
3.6
benzyl alcohol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
20
benzyl alcohol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.36
Butyraldehyde
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
19.7
Butyraldehyde
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
19.7
Butyraldehyde
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.2
Cyclohexanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
12.4
Cyclohexanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
3.1
ethanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
4.1
ethanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.82
Isobutyraldehyde
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
18.1
Isobutyraldehyde
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
29
Isobutyraldehyde
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.52
NAD+
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
3.4
NAD+
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
21
NAD+
Km above 50 mM, mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.38
NADH
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
10.3
NADH
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
34.8
NADH
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.21
trans-cinnamaldehyde
mutant enzyme W95L,, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
2.1
trans-cinnamaldehyde
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
18.9
trans-cinnamaldehyde
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.2
1-propanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
1.2
1-propanol
-
native enzyme, at 55°C
2.2
1-propanol
-
carboxymethylated enzyme, at 55°C
2.6
1-propanol
-
wild type enzyme, at 65°C
16.6
1-propanol
-
mutant enzyme N249Y, at 65°C
0.25
2-propanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.25
2-propanol
-
native enzyme, at 55°C
0.45
2-propanol
-
carboxymethylated enzyme, at 55°C
1.2
3-bromobenzyl alcohol
-
native enzyme, at 55°C
2
3-bromobenzyl alcohol
-
carboxymethylated enzyme, at 55°C
1.4
3-Methoxybenzyl alcohol
-
native enzyme, at 55°C
16
3-Methoxybenzyl alcohol
-
carboxymethylated enzyme, at 55°C
1.2
3-methoxybenzylalcohol
-
in the presence of 5 mM benzylalcohol, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
1.4
3-methoxybenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
1.1
4-bromobenzyl alcohol
-
native enzyme, at 55°C
3.5
4-bromobenzyl alcohol
-
carboxymethylated enzyme, at 55°C
0.7
4-carboxybenzaldehyde
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
0.7
4-carboxybenzaldehyde
-
native enzyme, at 55°C
0.6
4-methoxybenzaldehyde
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
0.6
4-methoxybenzaldehyde
-
native enzyme, at 55°C
3.3
4-methoxybenzaldehyde
-
wild type enzyme, at 65°C
20.5
4-methoxybenzaldehyde
-
mutant enzyme N249Y, at 65°C
1.5
4-methoxybenzyl alcohol
-
native enzyme, at 55°C
3.3
4-methoxybenzyl alcohol
-
wild type enzyme, at 65°C
20.5
4-methoxybenzyl alcohol
-
carboxymethylated enzyme, at 55°C
25.5
4-methoxybenzyl alcohol
-
mutant enzyme N249Y, at 65°C
4.6
4-nitrobenzaldehyde
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
4.6
4-nitrobenzaldehyde
-
native enzyme, at 55°C
1.8
benzaldehyde
-
carboxymethylated enzyme, at 55°C
3.5
benzaldehyde
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
3.5
benzaldehyde
-
native enzyme, at 55°C
11.3
benzaldehyde
-
wild type enzyme, at 65°C
25.5
benzaldehyde
-
mutant enzyme N249Y, at 65°C
1.4
benzyl alcohol
-
native enzyme, at 55°C
3.6
benzyl alcohol
-
wild type enzyme, at 65°C
14.5
benzyl alcohol
-
carboxymethylated enzyme, at 55°C
21.7
benzyl alcohol
-
mutant enzyme N249Y, at 65°C
0.65
Cyclohexanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.65
Cyclohexanol
-
native enzyme, at 55°C
1.2
Cyclohexanol
-
carboxymethylated enzyme, at 55°C
1.2
Cyclohexanol
-
wild type enzyme, at 65°C
15.9
Cyclohexanol
-
mutant enzyme N249Y, at 65°C
0.5
ethanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.5
ethanol
-
native enzyme, at 55°C
0.85
ethanol
-
carboxymethylated enzyme, at 55°C
1.4
NAD+
-
in 0.1 M glycine-NaOH, pH 9.2, at 65°C
1.4
NAD+
-
native enzyme, at 55°C
3.4
NAD+
-
wild type enzyme, at 65°C, in the presence of benzyl alcohol
18.5
NAD+
-
carboxymethylated enzyme, at 55°C
50
NAD+
-
mutant enzyme N249Y, at 65°C, in the presence of benzyl alcohol
2
NADH
-
carboxymethylated enzyme, at 55°C
4.5
NADH
-
in 50 mM Tris-HCl (pH 7.5) containing 0.25 mM benzaldehyde, at 65°C
4.5
NADH
-
native enzyme, at 55°C
10.3
NADH
-
wild type enzyme, at 65°C, in the presence of benzaldehyde
25.3
NADH
-
mutant enzyme N249Y, at 65°C, in the presence of benzaldehyde
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Thermostable NAD(+)-dependent alcohol dehydrogenase from Sulfolobus solfataricus: gene and protein sequence determination and relationship to other alcohol dehydrogenases
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Saccharolobus solfataricus
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Crystal structure of a ternary complex of the alcohol dehydrogenase from Sulfolobus solfataricus
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Saccharolobus solfataricus (P39462), Saccharolobus solfataricus
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Acta Crystallogr. Sect. D
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Saccharolobus solfataricus
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Saccharolobus solfataricus, Saccharolobus solfataricus DSM 1617
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Asn249Tyr substitution at the coenzyme binding domain activates Sulfolobus solfataricus alcohol dehydrogenase and increases its thermal stability.
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Saccharolobus solfataricus
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