6.5.1.B3: 2'-5' RNA ligase (GTP-activated)
This is an abbreviated version!
For detailed information about 2'-5' RNA ligase (GTP-activated), go to the full flat file.
Word Map on EC 6.5.1.B3
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6.5.1.B3
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byproduct
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8-hydroxyoctanoic
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arachidonic
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e-cadherin
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pyrococcus
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cox-catalyzed
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histone
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procaspase
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archaeon
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safer
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4-week
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heat-stable
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bxpc-3
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intensified
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four-week
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gemcitabine
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furiosus
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anti-tumor
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hyperthermophilic
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desaturases
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pro-apoptotic
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transwell
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cyclooxygenase-2
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parp
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2-series
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delta-6
- 6.5.1.B3
-
byproduct
-
8-hydroxyoctanoic
-
arachidonic
-
e-cadherin
-
pyrococcus
-
cox-catalyzed
- histone
-
procaspase
- archaeon
-
safer
-
4-week
-
heat-stable
-
bxpc-3
-
intensified
-
four-week
- gemcitabine
- furiosus
-
anti-tumor
-
hyperthermophilic
-
desaturases
-
pro-apoptotic
-
transwell
- cyclooxygenase-2
- parp
-
2-series
-
delta-6
Reaction
Synonyms
GTP-dependent RNA ligase, PF0027, PH0099
ECTree
6.5.1.B3 2'-5' RNA ligase (GTP-activated)Advanced search results
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results (Substrates Products
Substrates Products on EC 6.5.1.B3 - 2'-5' RNA ligase (GTP-activated)
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end
a mature tRNA molecule containing a 2'-5'-phosphodiester bond
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end
a mature tRNA molecule containing a 2'-5'-phosphodiester bond
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?
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end
a mature tRNA molecule containing a 2'-5'-phosphodiester bond
synthetic tRNA halves bearing 2',3'-cyclic phosphate and 5'-OH termini are substrates for the ligation reaction in vitro. The activity is dependent on GTP. The GTP structure is important but GTP hydrolysis is not required for the reaction, and GTPgammaS enhances the tRNA ligation activity. GTP is specific and indispensable for the tRNA ligation reaction. ATP, CTP, UTP, dATP, and dGTP cannot substitute for GTP. Neither GDP nor GMP enhance the ligation activity. The enzyme cleaves a 2',3'-cyclic phosphate group to produce a 2'-phosphate group at the 3' terminus of an RNA oligomer
the ligation product is unstable during in vitro reaction
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?
