6.5.1.8: 3'-phosphate/5'-hydroxy nucleic acid ligase
This is an abbreviated version!
For detailed information about 3'-phosphate/5'-hydroxy nucleic acid ligase, go to the full flat file.
Reaction
Synonyms
3'-P RNL, MXAN_0280, MXAN_4982, RNA-splicing ligase, rtcB, RtcB RNA ligase, Rtcb-1, RtcB1, Trl1, tRNA splicing ligase, tRNA-splicing ligase
ECTree
6.5.1.8 3'-phosphate/5'-hydroxy nucleic acid ligaseAdvanced search results
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results (Crystallization
Crystallization on EC 6.5.1.8 - 3'-phosphate/5'-hydroxy nucleic acid ligase
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CRYSTALLIZATION (Commentary) 
ORGANISM
UNIPROT
LITERATURE
in complex with GDP and Mg2+, sitting drop vapor diffusion method, using 0.2 M sodium dihydrogen phosphate, 20% (w/v) PEG3350
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three structures of RtcB complexes that capture snapshots along the entire guanylylation pathway. RtcB in complex with Mn(II) and GTP analogue guanosine 5'-(alpha-thio)-triphosphate shows that Mn1 is poised to stabilize the pentavalent transition state of guanylylation while a second manganese ion (Mn2) is coordinated to a nonbridging oxygen of the gamma-phosphoryl group. The diphosphate leaving group of 5'-(alpha-thio)-triphosphate is oriented apically to His404 with the epsilon nitrogen poised for in-line attack on the alpha phosphorus atom. The structure of RtcB in complex with 5'-(alpha-thio)-triphosphate also reveals the network of hydrogen bonds that recognize GTP and shows significant conformational changes accompanying the binding of the cofactor. A structure of the enzymic histidine-GMP intermediate depicts the end of the guanylylation pathway
crystal structures of RtcB in complex with Mn2+ alone and together with a covalently bound GMP, to 1.6 A and 2.3 A resolution, respectively. The RtcB/Mn2+ structure shows two Mn2+ ions at the active site, and an array of sulfate ions nearby that indicate the binding sites of the RNA phosphate backbone. The structure of the RtcB-GMP/Mn2+ complex reveals the detailed geometry of guanylylation of histidine 404. The enzyme's substrate-induced GTP binding site and the putative reactive RNA ends are in the vicinity of the binuclear Mn2+ active center
