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6.5.1.7: DNA ligase (ATP, ADP or GTP)

This is an abbreviated version!
For detailed information about DNA ligase (ATP, ADP or GTP), go to the full flat file.

Reaction

(deoxyribonucleotide)n-3'-hydroxyl
+
5'-(5'-diphosphoadenosine)-(deoxyribonucleotide)m
=
(deoxyribonucleotide)n+m
+
AMP

Synonyms

Hbu DNA ligase, multiple cofactor-dependent DNA ligase, Szi DNA ligase

ECTree

     6 Ligases
         6.5 Forming phosphoric-ester bonds
             6.5.1 Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)
                6.5.1.7 DNA ligase (ATP, ADP or GTP)

Temperature Stability

Temperature Stability on EC 6.5.1.7 - DNA ligase (ATP, ADP or GTP)

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
75
-
the enzyme reaches its maximum activity when incubated at 75°C for 1 h and gradually loses its activity thereafter
94
-
2.3 h, about 50% loss of activity
95
-
half-life: 2.6 h
99
-
half-life: 1.7 h